INCI: Leucojum Aestivum Bulb Extract
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1 INCI: Leucojum Aestivum Bulb Extract
2 INCI: Leucojum Aestivum Bulb Extract Slow down aging: DORMINs effect Boost natural skin defenses: enhance Super Oxide Dismutase (SOD) production Skin lightening: slowdown of melanocytes proliferation and melanin synthesis inhibition Anti-wrinkle: smoothen the skin and reduce wrinkles, relax muscle cells (Botox-like) *NEW* antiperspirant reduction in sweating (Botox-like)
3 SKIN AGING Skin extrinsic aging is the result of accumulated damage to skin due to several factors such as: UV and free radicals that both induce inflammation and damage the extracellular matrix including collagen and elastin fibers as well as the cell membranes; Muscle contractions subcutaneously create creases in the face: ruptures of the collagen and elastin fibers; Skin intrinsic aging: is the natural and programmed aging Hayflick limit theory is based on a finite number of cell replication; the higher the cell renewal, the shorter the cell life; Slowing down cell proliferation thus preserves the youth capital delaying the aging process This results in the appearance of wrinkles
4 DORMINs DORMINs are natural extracts of plants or plant organs in their dormant stage which are able to slow down cell proliferation: Maintain healthy, younger looking skin; Provide for skin protection; Prevent hyper proliferative cell disorders; Slowing down cell proliferation is one answer for anti-aging
5 INCI: Leucojum Aestivum Bulb Extract in vitro tests IBR-Snowflake slows down fibroblasts proliferation DORMIN-like dose dependent effect IBR-Snowflake slows down proliferation of normal human epidermal melanocytes (NHEM) and melanin synthesis lightens the skin IBR Snowflake significantly increases SOD (Super Oxide Dismutase) expression in keratinocytes (20% increase with 0.15% IBR-Snowflake); improving natural skin protection and reducing oxidative damages IBR-Snowflake at 1.5% effectively blocks muscle contraction in a muscle-nerve coculture for the duration of the study relaxes muscles like Botox
6 % Inhibition INCI: Leucojum Aestivum Bulb Extract DORMINs effect slowdown cell proliferation IBR-Snowflake inhibition of fibroblasts proliferation 90% 80% 70% 60% 50% 40% 30% 20% 10% 0% IBR-Snowflake Concetration (%)
7 INCI: Leucojum Aestivum Bulb Extract Skin whitening effect on melanocytes and melanogenesis Effect of IBR-Snowflake on the proliferation of normal human epidermal melanocytes (NHEM) and melanin synthesis Treatment IBR-Snowflake Kojic acid (positive control) Negative days Melanin synthesis MTT viability Melanin synthesis MTT viability Ccn. % % control % % control (%) inhibition inhibition
8 INCI: Leucojum Aestivum Bulb Extract Skin whitening effect on melanocytes and melanogenesis A concentration and time dependent effect on melanin synthesis in human normal epidermal melanocytes (NHEM) is observed with the IBR-Snowflake. The effect increases between day 3.5 and 7 in as low as 0.08% of the product to be 45% in day 3.5 and 70% inhibition in day 7. At the same time, an inhibitory effect on melanocytes proliferation is observed as well. The results serve as a strong support to the effect of IBR-Snowflake on skin whitening
9 INCI: Leucojum Aestivum Bulb Extract Effect on the natural antioxidant system Increase natural Super Oxide Dismutase (SOD) expression Naturally the skin eliminates oxidative stress through enzymes like SOD; IBR Snowflake increases significantly MnSOD in keratinocytes (*20% increase at 0.15% IBR- Snowflake); improving the natural skin protection and reducing oxidative damages * 0% 0.15% Mn SOD
10 INCI: Leucojum Aestivum Bulb Extract Effect on muscle contraction Test performed on co-culture of nerve and muscle cells, measuring the number of contractions compared to negative (culture media) and positive control (carisoprodol 5x10-3 M) Negative control Positive control IBR-Snowflake 1.5% T0 2h 6h 24h The IBR-Snowflake at 1.5% was effectively blocking muscle contraction in a co-culture of muscle-nerve for the duration of the study to be considered a long term effect.
11 INCI: Leucojum Aestivum Bulb Extract BOTOX LIKE AND SAFE HETCAM In vitro eye irritation Non-Irritant NRR Cytotoxicity Non-Cytotoxic Patch Test Cutaneous tolerance Non-Irritant Ames Test In vitro genotoxicity Non-Genotoxic HRIPT Skin sensitization Non Irritant, well tolerated by the skin and no allergic reaction IBR-Snowflake is demonstrated safe at least up to 15% recommended use level 0.5% 2%
12 INCI: Leucojum Aestivum Bulb Extract in vivo test - design CLINICAL EFFICACY AS ANTIPERSPIRANT Based on the precedent Botox-like anti wrinkle effect of IBR-Snowflake; and in analogy with the use of Botox as treatment against excessive sweating: antiperspirant effect was tested The study was a double-blind and intra-individual study. N=24, aged (average 30), all female, presenting strong perspiration in the armpit area defined as evaporation on both armpits 100mg/20min/armpit at C and 30-40%HR. 1% IBR-Snowflake cream was applied twice daily for 1 week, on the armpit area. Gravimetric measurement of sweating quantities was taken: cotton balls were weighed before and after being held under the armpit for a standardized period of time under standardized conditions of (sweat-inducing) temperature and humidity - the difference in weight corresponding to the amount of sweat released.
13 INCI: Leucojum Aestivum Bulb Extract in vivo test - results CLINICAL EFFICACY AS ANTIPERSPIRANT Based on the precedent Botox-like anti wrinkle effect of IBR-Snowflake; and in analogy with the use of Botox as treatment against excessive sweating: antiperspirant effect was tested 1% IBR-Snowflake cream The product containing 1% IBR-Snowflake provided an antiperspirant effect in comparison to initial state. This effect is characterized by a significant decrease of 36% of perspiration after seven days of repeated twice a day applications and 24 hours after single standardized application. The decrease of perspiration level of 212 mg of sweat on average on D8 (p=0.002). No relevant undesirable reaction was observed during the study.
14 INCI: Leucojum Aestivum Bulb Extract in vivo test - design CLINICAL EFFICACY STUDY-ANTI WRINKLE AND ANTI AGING Test done in France on 21 healthy female volunteers aged (median 60) Double blind placebo controlled Randomized hemi-face application IBR-Snowflake 2% concentration in a simple cream base, applied twice a day for 28 days Skin Image Analyser and PRIMOS measurements of the wrinkles
15 INCI: Leucojum Aestivum Bulb Extract in vivo results - after 28 days Number of micro relief furrows is decreased by 30%* and 86% of the panelists had a reduction; Number of medium depth wrinkles is decreased by 15%** and 62% of the panelists had a reduction; 0% -5% -10% -15% -20% -25% -30% Placebo Snowflake 2% furrows medium depth wrinkles *Results vs. placebo are highly significant p<0.05 **Results vs. placebo significant p<0.07
16 D0 INCI: Leucojum Aestivum Bulb Extract D0 D28 Improvement of the micro relief furrows and medium depth wrinkles with the use twice a day of a cream containing 2% IBR-Snowflake D28
17 INCI: Leucojum Aestivum Bulb Extract brings a new dimension to anti-aging and dryness: Slowdown aging: DORMINs effect Increase natural skin defense: enhance Super Oxide Dismutase (SOD) production Skin whitening: via slowdown of melanocytes proliferation and melanin synthesis inhibition Anti-wrinkle: smoothen the skin and reduces wrinkles, relaxes muscle cells (Botox-like) *NEW* antiperspirant reduction in sweating (Botox-like) USE IN: FACIAL CARE, BODY CARE, UNDER ARM
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