THE BEST WHITENING KNOWN
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1 THE BEST WHITENING KNOWN
2 In Vitro Evaluation of depigmenting activity on Melanocytes Coltures OLIVE ACTIVE HIDROXITIROSOL 4% vs. COMPETITORS Method: Cell cultures (melanocytes or protein) are treated with different brighteners: VitC, Arbutin, Kojic Acid and Olive Active Hidroxitirosol. Melanin Assay.9mg/ml Total Protein Assay.9 mg/ml
3 In Vitro Evaluation of depigmenting activity on Melanocytes Coltures OLIVE ACTIVE HIDROXITIROSOL 4% vs. COMPETITORS Method: Cell cultures (melanocytes or protein) are treated with different brighteners: VitC, Arbutin, Kojic Acid and Olive Active Hidroxitirosol. Melanin Assay.9mg/ml % of melanin inhibi-on % of melanin inhibi-on Concentra-on Control - Control + (Hydroquinone),145 67,7 OA Hidroxi-rosol 4%,9 37,8 % of melanin inhibi-on (per,9 mg/ml concentra-on) % of melanin Concentra-on inhibi-on Control -,9 Control + (Hydroquinone),9 42,2 OA Hidroxi-rosol 4%,9 37,8 % of protein inhibi-on % of protein Concentra-on inhibi-on Control - Control + (Hydroquinone),145 26,26 OA Hidroxi-rosol 4%,9 26,26 % of protein inhibi-on (per,9 mg/ml concentra-on) % of protein Concentra-on inhibi-on Control -,9 Control + (Hydroquinone),9 16,3 OA Hidroxi-rosol 4%,9 26, Control - Control + (Hydroquinone) OA Hidroxi-rosol 4% Total Protein Assay.9 mg/ml Control - Control + (Hydroquinone) OA Hidroxi-rosol 4%
4 In Vitro Evaluation of depigmenting activity on Melanocytes Coltures OLIVE ACTIVE HIDROXITIROSOL 4% vs. COMPETITORS Method: Cell cultures (melanocytes or protein) are treated with different brighteners: VitC, Arbutin, Kojic Acid and Olive Active Hidroxitirosol. Bright field microscope photographs of Melanocytes 4X Negative Control Vit C OA Hidroxitirosol 4% Arbutin Kojic Acid
5 In Vivo Evaluation of depigmenting activity on hyperpigmented spots OLIVE ACTIVE HIDROXITIROSOL 4% vs. VITAMIN C - 2 volunteers in which three skin spots are selected and treated with: 1. Untreated ( negative control) 2. OA HIDROXITIROSOL 4% at.2% 3. VITAMIN C 2% - Products applied twice a day during 56 days Melanin determination % of Melanin reduction t t14 t28 t56 Untreated - nega-ve control Cream with OA Hidroxi-rosol 4% at,2% Cream with Vitamin C at 2% Dt D14 Dt56 Olive Active Hidroxitirosol at.2% decreases melanin production by 18% after 56 days of treatment while Vitamin C at 2% decreases melanin production by 15%.
6 In Vivo Evaluation of depigmenting activity on hyperpigmented spots OLIVE ACTIVE HIDROXITIROSOL 4% vs. VITAMIN C Clinical evaluation: Hyperpigmented skin area visibility Evaluation criteria: - Absent = 1 - Slight = 2 - Fairly good = 3 - Good = 4 - Very Good = 5 OA Hidroxitirosol 4% at.2% Vitamin C at 2% t t14 t28 t56 t t14 t28 t56 Hyperpigmented skin area visibility decreases in the 75% of the volunteers after 56 days of use of a cream with OA Hidroxitirosol 4% at.2% (statistically significant) Hyperpigmented skin area visibility decreases in the 7% of the volunteers after 56 days of use of a cream with Vitamin C 2% (statistically significant)
7 In Vivo Evaluation of depigmenting activity on hyperpigmented spots OLIVE ACTIVE HIDROXITIROSOL 4% vs. VITAMIN C Clinical evaluation: Size and shape of the spots Evaluation criteria: - Absent = 1 - Slight = 2 - Fairly good = 3 - Good = 4 - Very Good = 5 OA Hidroxitirosol 4% at.2% Vitamin C at 2% t t14 t28 t56 t t14 t28 t56 Spots size and shape decreases in the 8% of the volunteers after 56 days of use of a cream with OA Hidroxitirosol 4% at.2% (statistically significant) Spots size and shape decreases in the 75% of the volunteers after 56 days of use of a cream with Vitamin C at 2% (statistically significant)
8 In Vivo Evaluation of depigmenting activity on hyperpigmented spots OLIVE ACTIVE HIDROXITIROSOL 4% vs. VITAMIN C Photographs of selected spots OA HIDROXITIROSOL 4% AT.2% Bio Basic Europe s.r.l. Bio Basic Europe s.r.l. t t56
9 In Vivo Evaluation of depigmenting activity on hyperpigmented spots OLIVE ACTIVE HIDROXITIROSOL 4% vs. VITAMIN C Photographs of selected spots Bio Basic Europe s.r.l. Bio Basic Europe s.r.l. OA HIDROXITIROSOL 4% AT.2% t t56
10 In Vivo Evaluation of depigmenting activity on hyperpigmented spots OLIVE ACTIVE HIDROXITIROSOL 4% vs. VITAMIN C Photographs of selected spots VITAMIN C AT 2% Bio Basic Europe s.r.l. Bio Basic Europe s.r.l. t t56
11 EG ACTIVE COSMETICS S.L. Avda Digonal 539, 4º1º 829 Barcelona (Spain) Tel: Fax: info@egacgvecosmegcs.com
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