Primers used for real time qpcr
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1 Supplementry Tble 1. Primers used for rel time qpcr Gene Accession number Forwrd/reverse primers Tgfα Tgfβ1 Hgf Cyclin A2 Cyclin B1 Cyclin D1 Cyclin E1 FoxM1 p21 Lrt Cyp26A1 CrbpI Rrβ Bcmo1 Bcmo2 NM_31199 NM_11577 NM_1427 NM_9828 NM_17231 NM_7631 NM_7633 NM_821 NM_7669 NM_23624 NM_7811 NM_11254 NM_11243 NM_21486 NM_ '-CCATCAGTCTACCCTTTGC 5'-GGAGAAGGTACACAGCG 5'-GGCTACCATGCCAACTT 5'-ACCCACGTAGTAGACGA 5'-AAATGAGAATGGTTCTTGGTG 5'-CTGGCCTCTTCTATGGCT 5'-GGCTGACACTCTTTCCG 5'-CTGGTAGCAAGAATTAGAGCAT 5'-AGCAAATATGAGGAGATGTACC 5'-CGACTTTAGATGCTCTACGGA 5'-TCCCAGACGTTCAGAACC 5'-AGGGCATCTGTAAATACACT 5'-TGCACCAGTTTGCTTATGTT 5'-CCGTGTCGTTGACATAGG 5'-CTTACTGCCCTTTCCTCG 5'-GAACACTATCAGAGAAACACCTAAT 5'-GGTTCCTTGCCACTTCTT 5'-GAGTCGGGATATTACGGTTG 5'-AGTTCAAGACTAGCCTGCTCA 5'-TACAAGCTGGCCTTCGAC 5'-AGAGCAATCAAGACAACAAGTTAG 5'-ATCGCAGGGTCTCCTTAAT 5'-AATGAGAGCTGAGGGTG 5'-GGTATGCGTTTCGGTCC 5'-CAAGTTCAAGTGGGAATATAGCAGA 5'-ACTGACTGACTCCACTGTT 5'-ATGAAATGTGGAGAAGACTTCTAT 5'-CTTTGTCCACGACGGAT 5'-AGGACCAGGGCTGTATTGTG 5'-CGCTGGCTGAAGAATAGGAC
2 Pprα Pprβ/δ Pprγ Cb1 Cb2 Pdk4 αsm Col1A1 18S NM_11144 NM_11145 NM_11146 NM_7726 NM_9924 NM_13743 NM_7392 NM_7742 5'-ACTGGATGACAGTGACATTTC 5'-CTCCTGCAACTTCTCAATGTAG 5'-GGGTCAGAAGCTAGACAGCGAG 5'-AGAAAGAACAGCTATGGTCCAGTTCC 5'-TCTCCAGCATTTCTGCTCCACAC 5'-CAGGTTCTACTTTGATCGCACTTTGGTAT 5'-CGTGTTCCACCGCAAAGATAGTC 5'-GTACCTGTCGATGGCCGT 5'-TGTGGGTCCTCTCAGCATTGATT 5'-CCAGCCCAGTAGGTAGTCGTT 5'-TCGAACTCTTCAAGAATGCC 5'-GGTCAGTAATCCTCAGAGGAACA 5'-TCTGGCACCACTCTTTCTATAAC 5'-TAGCCACATACATGGCGG 5'-CCTCAGAAGAACTGGTACATCA 5'-GGCCTCGGTGGACATTA 5'-CCATCCAATCGGTAGTAGCG 5'-GTAACCCGTTGAACCCCATT
3 4 Lrt -/- 3 ALT, U/L Supplementry Figure 1. Serum lnine minotrnsferse (ALT) ctivity for wild type nd Lrt -/- mice following shm opertion. Vlues re given s the men ± 1 S.D., n = 1 for ech time nd genotype.
4 Reltive mrna/18s Hgf b c b, c b, c c Lrt -/- c Reltive mrna/18s Tgfα b b b Lrt -/ Reltive mrna/18s Tgfβ Lrt -/ Supplementry Figure 2. Hgf, Tgfα nd Tgfβ mrna expression in the livers of wild type nd Lrt -/- mice following PHE. Vlues re given s the men ± 1 S.D., n = 1 for ech genotype. Vlues mrked with different letters (, b, c) re significntly different, P <.5.
5 .7.6 Lrt -/- Reltive mrna/18s Il-6 Tnfα Supplementry Figure 3. Il-6 nd Tnfα mrna expression in the livers of wild type nd Lrt -/- mice 12 h following PHE. Vlues re given s the men ± 1 S.D., n = 1 for ech genotype.
6 Totl Heptic ROH, µg/g PHE Lrt -/- PHE Shm Lrt -/- Shm Supplementry Figure 4. Heptic retinol levels in wild type nd Lrt -/- mice following PHE nd shm opertion. Vlues re given s the men ± 1 S.D., n = 1 for ech time nd genotype.
7 A. Lrt -/- hours 12 hours 72 hours B. Lrt -/- hours 12 hours 72 hours Supplementry Figure 5. Heptic triglyceride ccumultion in livers of wild type nd Lrt -/- mice following PHE. Pnel A. Representtive H&E stined liver sections from wild type nd Lrt -/- mice,, 12, nd 72 h fter PHE. Pnel B. Representtive oil red O stined liver sections from wild type nd Lrt -/- mice,, 12, nd 72 h fter PHE.
8 3 25 Shm Lrt -/- Shm Totl Heptic TG, mg/g Supplementry Figure 6. Heptic triglyceride concentrtions for wild type nd Lrt -/- mice following shm opertion. Vlues re given s the men ± 1 S.D., n = 1 for ech time nd genotype.
9 Absence of Heptic Retinoid Stores Diminished heptic ll-trns-retinoic cid concentrtions Acute Heptic Injury Direct (Erly) Effects Impired retinoic cidresponsive gene expression Lessened Hgf, Tgfα, Pdk4, p21, nd Pprb/d expression Less AEA synthesis Indirect (Lte) Effects Temporl dely in the rise in Cyclin D1, E1, A2, B1 nd FoxM1 expression Altered recovery of serum retinol concentrtion nd other retinoidrelted prmeters Impired prolifertive responses resulting in dely in liver weight regin within the first 48 hr following heptectomy Supplementry Figure 7. Summry of the effects identified in Lrt -/- mice which give rise to impired liver regenertion.
10 Blood glucose, mg/dl PHE Lrt -/- PHE Shm Lrt -/- Shm Supplementry Figure 8. Blood glucose levels in wild type nd Lrt -/- mice following PHE nd shm opertion. Vlues re given s the men ± 1 S.D., n = 1 for ech time nd genotype.
2018 American Diabetes Association. Published online at
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