16 Sep 2016 Postdoctoral fellow at the Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, (Director: Prof.

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1 Curriculum vitae Name Holger Winkels, Ph.D. Office address Division of Inflammation Biology La Jolla Institute for Allergy & Immunology 9420 Athena Circle Drive, La Jolla, CA, OCRID Place of birth Geilenkirchen, Germany Date of birth 02 Oct Citizenship German Languages German (native) English (proficient) Latin (Latinum) Dutch (basic) Core capabilities Expertise in cardiovascular and immunological research obtained during extensive work at international renown institutes (IPEK, LMU, Munich, Germany; Department of Medical Biochemistry, AMC, Amsterdam, The Netherlands: La Jolla Institute for Allergy and Immunology, La Jolla, USA) contributing substantially to progress in the field of cardiovascular research. Broad knowledge and application of mouse models used in atherosclerosis and immunology related research further complemented by expertise in flow cytometry, mass cytometry, single-cell RNA-sequencing, cell culture, and various imaging techniques (CLSM, TPLSM, STED). Education 16 Sep 2016 Postdoctoral fellow at the Division of Inflammation Biology, La Jolla now Institute for Allergy and Immunology, (Director: Prof. Klaus Ley); 10 Jan 2017 PhD Disputation (Dr.rer.nat.) in medical sciences ( magna cum laude ) 01 July 2011 Ph.D. student at the Institute for Cardiovascular Prevention (IPEK), 31 July 2016 Ludwig-Maximilian-University (LMU) Munich, (Director Prof C. Weber) Topic: The role of the co-stimulatory CD27/CD70 axis in atherosclerosis Supervisors: Prof. Dr. Alexander Faussner Prof. Dr. Esther Lutgens, MD Dr. rer. nat. Norbert Gerdes 01 May 2012 Guest researcher at the Department for Medical Biochemistry, Academic 31 Jan 2016 Medical Center (AMC) Amsterdam, The Netherlands

2 01 Aug Research visit at Department of Immunology, Harvard Medical 17 Dec School, Boston, USA (Director: Prof. Ulrich von Andrian); Title: In vivo imaging of signal transduction during the induction of an adaptive immune response Diploma thesis (Biology) Institute for Molecular Cardiovascular Research (IMCAR), RWTH Aachen, (Director: Prof. C. Weber); Title: T cell homeostasis in atherosclerosis Studies of Biology at Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Germany, mark: very good Honors 10 July 2018 Invitation as candidate for end-round of the associate professorship (W2 Professur) of cardiovascular immunology, Technical University Munich, Germany 7 June 2018 Article about my scientific background in the category Meet the first authors, Circulation Research, 2018;122: June 2018 The cover of the June 8, 2018, Volume 122, Issue 12 of Circulation research was taken from a publication figure of Winkels et al Circ Res June 2018 Editorial comment to Winkels et al Circ Res 2018, Cochain et al Circ Res 2018, by Ruth Williams in the June 8, 2018, Volume 122, Issue 12 of Circulation Research 7 June 2018 Editorial comment to Winkels et al Circ Res 2018, Cochain et al Circ Res 2018, by Hanrui Zhang, Nancy R. Zhang, Mingyao Li, and Muredach P. Reilly in the June 8, 2018, Volume 122, Issue 12 of Circulation Research 27 May 2018 Article about Winkels et al Circ Res 2018 by Gomes M. Scientists identify new leukocyte populations linked to atherosclerosis, 27 April 2018 Organizer of AYOXXA s LUNARIS Protein Multiplexing Seminar La Jolla Institute for Allergy and Immunology, CA, USA 18 April 2018 Invitation to the cardiovascular lecture series of the University of Cologne, Department of Cardiology 10 April 2018 Research highlight comment to Winkels et al Circ Res 2018, Cochain et al Circ Res 2018, by Fernandez-Ruiz I. Redefining leukocytes in atherosclerosis, Nature Reviews Cardiology, 5, 319 (2018) doi: /s April 2018 Online and print article about my scientific background and my persona in the category Up and Coming in the La Jolla Institute magazine ImmuneMatters, Spring Issue February 2018 Organizer of Miltenyi s Seminar Introducing REAlease technology La Jolla Institute for Allergy and Immunology, CA, USA 22 Nov 2017 Editorial comment to Winkels H. et al, EHJ 2017, 38(48): by Stein S., Matter C. Protective role of the co-stimulator CD27 receptor and regulatory T cells un early atherosclerosis EHJ, 38(48):

3 19 Oct 2017 Invitation as candidate for end-round of the Neven DuMont associate professorship (W2 Professur) of experimental cardiology of aging, University of Cologne, Germany 18 June 2017 Elected as new chair of the Gordon Research Seminar for Atherosclerosis June 2017 Discussion Leader, Session: Lipids, Macrophages, and Inflammation Gordon Research Seminar Atherosclerosis, Newry, Maine, USA 21 April 2017 Invitation to the Invitation to UCSD Cardiovascular Science Conference (AVB) Series, San Diego, California, USA April 2016 Invitation and participation to the Cardiovascular Research Workshop at Bayer March 2016 The cover of the March 2016, Volume 73, Issue 5 of Cellular and Molecular Life Sciences was taken from a publication figure of Spitz et al, CMLS. Awards 08 March year DFG fellowship Unraveling Treg Plasticity in Atherosclerosis GZ WI 4811/1-1 3 Jan 2018 SPARK Award La Jolla Institute for Allergy and Immunology (25000 Dollar) 6 Dec 2017 Travel Fellowship Next Generation Immunology February 2018, Rehovot, Israel 30 Nov 2017 Spot Award La Jolla Institute for Allergy and Immunology 14 May 2017 AAI Immunology 2017 Trainee Abstract Award 29 May 2016 Young Investigator Fellowship Award, EAS Innsbruck June 2015 Travel Award Gordon Research Conference Atherosclerosis 25 July 2013 DZHK Trainee Program Travel Support 22 March 2013 Travel Grant Boehringer Ingelheim Foundation Memberships 2018 present Member: German Society for Atherosclerosis (DGAF) 2017 present Member: American Heart Association 2017 present Member: La Jolla Institute for Immunology and Allergy Postdoc Association, representative of the Ley lab 2017 present Member: The American Association of Immunologists 2016 present Member: European Atherosclerosis Society (EAS) 2012 present Member: German Society for Immunology (DGFI) Student representative Integrated Research Training Group (IRTG) Collaborative Research Center (CRC) 1054 Control and plasticity of cell fate decisions in the immune system Member: CRC1054 Control and plasticity of cell-fate decisions in the immune system Scientific activities and experience

4 2012 present Reviewer for original articles at PloS One, Atherosclerosis, Thrombosis and Hemostasis, Circulation Research, Basic Research in Cardiology, Cardiovascular Research, Neurological Research, International Journal of Cardiology, JEM, Journal of Leukocyte Biology, JCI Co-applicant and supervisor of animal ethical application GZ Participation in following courses: Modern Biochemistry of Cardiovascular disease Light Microscopy of Small Arterial Vessels Flow cytometric immunophenotyping Laboratory animal course according to FELASA B guidelines Teaching and Supervision Co-supervision of a Masters student (Charlotte Spitz), LMU Munich Co-supervision of a Medical student (Maiwand Ahmadsei), LMU Munich Teaching of technical assistants (5), international laboratory visitors (3), and medical students (2) in cardiovascular and immunological research related methods. Selected oral presentation End-round of the recruitment symposium for the associate professorship (W2 Professur) of cardiovascular immunology, Technical University Munich, Germany, 10 th July 2018 Title: The immune cell atlas in mouse atherosclerosis La Jolla Institute In-house Seminar Series, CA, USA 24 th May 2018 Title: The immune cell landscape in atherosclerosis new players on the field Vascular Discovery: From Genes to Medicine Scientific Sessions 2018 (ATVB), San Francisco, CA, USA, May 2018 Title Comprehensive Assessment of Immune Cells in Mouse and Human Atherosclerosis by Single-cell RNA-sequencing and Mass Cytometry University of Cologne, Department of Cardiology, Germany, 18 th April 2018 Title: Antigen-specific CD4 T cells and Vaccination in Atherosclerosis End-round of the recruitment symposium for the Neven DuMont associate professorship (W2 Professur) of experimental cardiology of aging, University of Cologne, Germany, 19 th October 2017 Title: Adaptive and Innate Immune Cells in Atherosclerosis Gordon Research Conference Atherosclerosis, Newry, Maine, USA, June 2017 Title: Leukocyte Inventory In Atherosclerotic Mouse Aortas Immunology 2017, Washington, D.C., USA, May 2017

5 Title: CD27 co-stimulation increases regulatory T cell survival and limits progression of atherosclerosis Invitation to UCSD Cardiovascular Science Conference (AVB) Series, San Diego, California, USA, 21 April 2017 Title: The role of the co-stimulatory CD27/CD70 dyad in atherosclerosis 84 th EAS Congress, Innsbruck, Austria, 29 May-1 June 2016 Title: CD27 co-stimulation fosters regulatory T cell survival and ameliorated progression of atherosclerosis Gordon Research Conference Atherosclerosis, Newry, Maine, USA, June 2015 Title: Co-stimulation via CD27 increases frequency of regulatory T cells and ameliorates atherogenesis in hyperlipidemic mice Gordon Research Seminar Atherosclerosis, Newry, Maine, USA, June 2015 Title: Co-stimulation via CD27 increases frequency of regulatory T cells and ameliorates atherogenesis in hyperlipidemic mice Selected poster presentation Immunology 2018, Austin, Tx, USA, 4-8 May 2018 Title: Next generation analysis of inflammatory infiltrates in atherosclerosis by mass cytometry and single cell RNA-sequencing Next Generation Immunology Symposium, Rehovot, Israel, 12 Feb 2018 Title: Next generation analysis of inflammatory infiltrates in atherosclerosis by mass cytometry and single cell RNA-sequencing Gordon Research Conference Atherosclerosis, Newry, Maine, USA, June 2017 Title: Leukocyte Inventory In Atherosclerotic Mouse Atherosclerotic Mouse Aortas Immunology 2017, Washington, D.C:, USA, May 2017 Title: CD27 co-stimulation increases regulatory T cell survival and limits progression of atherosclerosis Cardiovascular Research Bayer, Heiligenhaus, Germany April, 2016 Title: Deficiency of the co-stimulatory molecule CD27 impairs Treg survival and exacerbates atherosclerosis Gordon Research Conference Atherosclerosis, Newry, Maine, USA, June 2015 Title: Co-stimulation via CD27 increases frequency of regulatory T cells and ameliorates atherogenesis in hyperlipidemic mice 17 th International Symposium on Atherosclerosis, Amsterdam, The Netherlands, May 2015

6 Title: Deficiency of the co-stimulatory molecule CD27 impairs Treg survival and exacerbates atherosclerosis ATVB/PVD Arteriosclerosis, Thrombosis and Vascular Biology/Peripheral Vascular Disease, San Francisco, California, USA, 7-9 May 2015 Title: Deficiency of the co-stimulatory molecule CD27 impairs Treg development and exacerbates atherosclerosis Midwinter conference, Advances in Immunobiology, Seefeld, Austria, January, Title: Deficiency of the co-stimulatory molecule CD27 impairs Treg survival and exacerbates atherosclerosis Gordon Research Conference Atherosclerosis, Stowe, Vermont, USA, June 2013 Title: Pharmacological inhibition of CD70 hampers progression of atherosclerosis World Immune Regulation Meeting (WIRM) VI, Davos, Switzerland, March 2012 Title: T cell homeostasis in atherosclerosis Published original Articles Kobijama K, Vassallo M, Mitzi J, Winkels H, Kimura T, Miller J, Wolf D, Ley. K. A clinically applicable adjuvant for an atherosclerosis vaccine. European Journal of Immunology. June 2018; doi: /eji Winkels H*, Ehinger E*, Vassallo M, Buscher K, Dinh H, Kobijama K, Hamers AJA, Cochain C, Vafadarnejad E, Saliba AE, Zernecke A, Bala PA, Ghosh AK, Michel NA, Hoppe N, Hilgendorf I, Zirlik A, Hedrick C, Ley K, Wolf D. Atlas of the Immune Cell Repertoire in Mouse Atherosclerosis Defined by Single-Cell RNA-Sequencing and Mass Cytometry. Circulation research. 2018;122: doi: /CIRCRESAHA *, These authors contributed equally. Cochain C, Vafadarnejad E, Arampatzi P, Jaroslav P, Winkels H, Ley K, Wolf D, Saliba AE, Zernecke A. Single-cell RNA-seq reveals the transcriptional landscape and heterogeneity of aortic macrophages in murine atherosclerosis. Circulation Research. 2018;122: doi: /CIRCRESAHA Kimura T*, Kobiyama K*, Winkels H*, Tse K, Miller J, Vassallo M, Wolf D, Ryden C, Dileepan T, Jenkins M, James EA, Kwok WW, Hanna DB, Kaplan RC, Strickler H, Durkin H, Kassaye S, Karim R, Tien P, Landay A, Gange S, Signey J, Sette A, Ley K. Regulatory CD4 + T cells recognizing MHC-II-restricted peptide epitoes of apolipoprotein B. Circulation doi: /CIRCULATIONAHA *These authors contributed equally. Kusters P, Seijkens T, Beckers L, Lievens D, Winkels H, de Waard V, Duijvestain A, Liljeqvist ML, Roy J, Daugherty A, Newby A, Gerdes N, Lutgens E. CD40L deficiency

7 protects against anurysm formation. Arteriosclerosis, Thrombosis, and Vascular Biology. 2018;38: doi: /ATVBAHA Wolf D, Michel NA, Blankenbach H, Wiedemann A, Buscher K, Hohmann JD, Lim B, Baeuml M, Marki A, Mauler M, Duerschmied D, Fan Z, Winkels H, Sidler D, Diehl P, Zajonic D, Hilgendorf I, Stachon P, Marchini T, Willecke F, Schell M, Sommer B, von zur Muhlen C, Reinoehl J, Gerhardt T, Plow EF, Yakubenko V, Libby P, Bode C, Ley K, Peter K, Zirlik A. A ligand-specfifc blockade oft he integrin Mac-1 (Cd11b/CD18) slectively targets pathologic inflammation while maintaimning protective host-defense. Nature Communications Feb 6;9(1):525. doi: /s Kusters P, Smeets E, Legein B, Seijkens T, Winkels H, Gijbels M, Buerger C, Barthels C, Biessen E, Weber C, Brocker T, Gerdes N, Lutgens E. Constitutive CD40-signaling in Dendritic Cells limits Atherosclerosis by provoking inflammatory bowel Disease and ensuing Cholesterol Malabsorption. The American Journal of Pathology Dec;187(12): doi: /j.ajpath Winkels H, Meiler S, Smeets E, Lievens D, Engel D, Spitz C, Buerger C, Beckers L, Dandl A, Reim S, Ahmadsei M, Hartwig H, Holdt LM, Hristov M, Megens RTA, Schmitt MM, Biessen EA, Borst J, Faussner A, Weber C, Lutgens E, Gerdes N. CD27 costimulation increases frequency of regulatory T cells and reduces atherosclerosis in hyperlipidemic mice. European Heart Journal Dec 21;38(48): doi: /eurheartj/ehx517 Winkels H*, Meiler S*, Smeets E*, Lievens D, Engel D, Spitz C, Buerger C, Rinne P, Beckers L, Dandl A, Reim S, Ahmadsei M, Van den Bossche J, Megens RTA, Schmitt MM, de Winther M, Biessen EA, Borst J, Faussner A, Weber C, Lutgens E, Gerdes N. CD70 deficiency impairs macrophage cholesterol uptake and aggravates atherosclerosis by inducing a hypo-active macrophage phenotype. Thrombosis and Haemostasis Jan 5;117(1): doi: /TH *These authors contributed equally. Jung C, Lichtenauer M, Strodthoff D, Winkels H, Wernly B, Buerger C, Kamchybekov U, Lutgens E, Figulla HR, Gerdes N. Alterations in systemic levels of Th1, Th2, and Th17 cytokines in overweight adolescents and obese mice. Pediatric Diabetes Sep 6. doi: /pedi Meiler S, Smeets E, Winkels H, Shami A, Pascutti FM, Nolte M, Beckers L, Weber C, Gerdes N, Lutgens E. Constitutive GITR Activation Reduces Atherosclerosis by Promoting Regulatory CD4+ T cell responses. Arteriosclerosis, Thrombosis, and Vascular Biology Jul 21. doi: /ATVBAHA Gerdes N, Seijkens T, Lievens D, Kuijpers MJE, Winkels H, Projahn D, Hartwig H, Beckers L, Megens RTA, Boon L, Noelle RJ, Soehnlein O, Heemskerk JWM, Weber C, Lutgens E. Platelet CD40 exacerbates atherosclerosis by transcellular activation of endothelial cells and leukocytes. Arteriosclerosis, Thrombosis, and Vascular Biology. Mar;36(3): Jan 28 Doi: /ATVBAHA

8 Chatzigeorgiou A, Seijkens T, Zarzycka B, Engel D, Poggi M, van den Berg S, van den Berg S, Soehnlein O, Winkels H, Beckers L, Lievens D, Driessen A, Kusters P, Biessen E, Garcia-Martin R, Klotzsche-von Ameln A, Gijbels M, Noelle R, Boon L, Hackeng T, Schulte KM, Xu A, Vriend G, Nabuurs S, Chung KJ, Willems van Dijk K, Rensen PC, Gerdes N, de Winther M, Block NL, Schally AV, Weber C, Bornstein SR, Nicolaes G, Chavakis T, Lutgens E. Blocking CD40-TRAF6 signaling is a therapeutic target in obesity-associated insulin resistance. Proceedings of the National Academy of Sciences of the United States of America Feb 18;111(7): doi: /pnas Lievens D, Habets KL, Robertson AK, Laouar Y, Winkels H, Rademakers T, Beckers L, Wijnands E, Boon L, Mosaheb M, Ait-Oufella H, Mallat Z, Flavell RA, Rudling M, Binder CJ, Gerdes N, Biessen EA, Weber C, Daemen MJ, Kuiper J, Lutgens E. Abrogated transforming growth factor beta receptor II (TGFβRII) signalling in dendritic cells promotes immune reactivity of T cells resulting in enhanced atherosclerosis. European Heart Journal. (2012), doi: /eurheartj/ehs106 Review Articles Winkels H, Ehinger E, Ghosesh Y, Wolf D, Ley K. Atherosclerosis in the single cell era Current Opinion in Lipidology. Accepted Pfeiler S, Winkels H, Kelm M, Gerdes N. IL-1 family cytokines in cardiovascular disease. Cytokine. Nov 30. pii: S (17) doi: /j.cyto Ley K, Gerdes N, Winkels H. How Costimulatory and Coinhibitory Pathways Shape Atherosclerosis. Arteriosclerosis, Thrombosis, and Vascular Biology. Mar; 2017; ATVBAHA doi.org/ /atvbaha Spitz C, Winkels H, Buerger C, Weber C, Lutgens E, Hansson GK, Gerdes N. Regulatory T cells in atherosclerosis: Essential immune regulatory function and therapeutic potential. Cellular and Molecular Life Sciences. Mar; (5): doi: /s Letters, editorials Winkels H, Ley K. Natural Killer cells at ease Atherosclerosis is not affected by genetic depletion or hyperactivation of Natural Killer cells. Circulation Research Jan 5;122(1):6-7. doi: /CIRCRESAHA Winkels H, Weber C, Lutgens E, Gerdes N. Atherosclerosis: cell biology and lipoproteins focus on inkt cells and CD40/CD40L in atherosclerosis and metabolic disorders. Current Opinion in Lipidology 2014 Oct;25(5): doi: /MOL

9 Gerdes N, Winkels H, Weber C. Atherosclerosis: cell biology and lipoproteins focus on anti-inflammatory mechanisms as therapeutic options. Current Opinion in Lipidology 2013 Apr;24(2): doi: /MOL.0b013e32835ec608