Cardiovascular research solutions. Conveniently groundbreaking

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Sydney Sutton
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1 Cardiovascular research solutions Conveniently groundbreaking

2 The easy workflow to pure cardiac cell types Easily dissociate neonatal hearts for the recovery of all major cardiac cell types Gentle yet effective dissociation of cardiac tissue with no hands-on time Conserved cell surface epitopes Process to 6 neonatal hearts per hour using optimized protocols Neonatal Heart Dissociation Kit for the efficient digest of neonatal hearts. Extracted and washed neonatal hearts. Get pure neonatal cardiac cell populations using MCS Technology Premium recovery of cell populations Gentle isolation of viable and functional cells Most-cited cell separation technology Magnetic labeling of all non-cardiomyocytes with specific MCS Microeads. Neonatal hearts and enzymes are placed in a gentlemcs C Tube. Manual cell separation. Magnetically labeled cells are retained within the column placed in a MCS Separator while non-labeled cardiomyocytes are collected in the flow-through. For fully automated cell isolation use the automcs Pro Separator. Mechanical and enzymatic tissue dissociation on the gentlemcs Octo Dissociator with Heaters for the recovery of functional cardiac cells. Plated neonatal cardiomyocytes after cell isolation. Miltenyi iotec offers further solutions for each step of your cardiac research, including research on adult heart tissue and ES/iPS derived cardiac cells. See for yourself at

From tissue to pure cells with MCS Solutions Walkaway neonatal heart dissociation set it and forget it Neonatal Heart Dissociation Kit Process your neonatal heart specimens quickly, easily, and above

Obtain functional cardiomyocytes, cardiac fibroblasts, cardiac endothelial cells, and cardiac progenitor cells Conserve cell surface markers for subsequent cell isolation Combine with the gentlemcs

ased on proven MCS Technology, these kits enable efficient isolation of cardiac cells, including cardiomyocytes, cardiac fibroblasts, and cardiac endothelial cells from single-cell suspensions

3 From tissue to pure cells with MCS Solutions Walkaway neonatal heart dissociation set it and forget it Neonatal Heart Dissociation Kit Process your neonatal heart specimens quickly, easily, and above all, gently. Get excellent yields of viable, single-cell suspensions in just one hour. Obtain functional cardiomyocytes, cardiac fibroblasts, cardiac endothelial cells, and cardiac progenitor cells Conserve cell surface markers for subsequent cell isolation Combine with the gentlemcs Dissociator family for a walkaway, standardized solution Easy cell isolation with neonatal cardiac cell isolation kits Follow gentle heart dissociation with gentle cell isolation. ased on proven MCS Technology, these kits enable efficient isolation of cardiac cells, including cardiomyocytes, cardiac fibroblasts, and cardiac endothelial cells from single-cell suspensions generated on the gentlemcs Dissociators. Isolate cardiac cell populations with up to 98% purity in under an hour Get cardiomyocytes, cardiac fibroblasts, cardiac endothelial cells, and cardiac progenitor cells Increase your output with automated isolation protocols Cell count/heart Cell count/heart Viability Viability (%) Sensitive neonatal cardiomyocyte isolation The Neonatal Cardiomyocyte Isolation Kits enable untouched isolation of functional cardiomyocytes from dissociated neonatal mouse and rat hearts. ll non-cardiomyocytes are magnetically labeled using a MCS Microead cocktail, then retained within the MCS Column during separation, while the unlabeled cardiomyocytes are collected in the flow-through fraction. Figure : Neonatal hearts from mice and rat (P-P3) were dissociated using the gentlemcs Octo Dissociator with Heaters and the Neonatal Heart Dissociation Kit, resulting in high yields of single cells with excellent viability. gentlemcs Dissociators t the push of a button, get viable, single-cell suspensions or homogenates from up to 8 tissue samples, whether processed in parallel or sequentially. Work with neonatal heart dissociation programs optimized for the specific challenges faced by cardiovascular researchers, or enjoy the flexibility to easily set up your own programs. Percentage α-actinin-fitc ³ ² ¹ efore separation Forward scatter ³ ² ¹ Cardiomyocytes Purity Recovery Figure 2: The gentlemcs Dissociator family, including the gentlemcs Octo Dissociator, the gentlemcs Octo Dissociator with Heaters, and the gentlemcs Dissociator. Figure 3: Cardiomyocytes were isolated from dissociated neonatal mouse and rat hearts (P P3) using the respective Neonatal Cardiomyocyte Isolation Kit. Flow analysis data is exemplarily shown for rat cells (). The immunomagnetic isolation resulted in high yields of viable cardiomyocytes with purities of up to 98% ().

Pure cardiac cell populations in a heartbeat Efficiently isolate mouse or rat cardiac endothelial cells Using the respective

endothelial cells ready for your downstream applications.

immunofluorescent staining of mouse cells before () and after isolation ().

Work with pure cardiac fibroblasts See the difference when you use the Cardiac Fibroblast Isolation Kits to isolate viable

Cardiac fibroblasts are isolated in a two-step magnetic cell separation procedure, by depletion of all non-target cells

2-PC 8 6 4 2 ³ ² ¹ efore separation CD3-PE ¹ ² ³ ³ ² ¹ Endothelial cells ¹ ² ³ Percentage 8 6 4 2 C Purity D Recovery Purity

Purities of up to 96% were obtained using the Neonatal Cardiac Fibroblast Isolation Kit ().

Figure 6: Cardiac endothelial cells were isolated from dissociated neonatal mouse hearts (P P3) using the Neonatal Cardiac

4 Pure cardiac cell populations in a heartbeat Efficiently isolate mouse or rat cardiac endothelial cells Using the respective Cardiac Endothelial Cell Isolation Kit you efficiently remove unwanted cells from your sample, leaving viable cardiac endothelial cells ready for your downstream applications. Figure 4: Cardiomyocytes were successfully enriched using the Neonatal Cardiomyocyte Isolation Kit, as shown by immunofluorescent staining of mouse cells before () and after isolation (). Cells were stained for α-actinin (green) and vimentin (red). The blue stain (DPI) denotes the nuclei. Work with pure cardiac fibroblasts See the difference when you use the Cardiac Fibroblast Isolation Kits to isolate viable mouse and rat cardiac fibroblasts. Cardiac fibroblasts are isolated in a two-step magnetic cell separation procedure, by depletion of all non-target cells followed by the enrichment of neonatal cardiac fibroblasts. Percentage CD45/CD9.2-PC ³ ² ¹ efore separation CD3-PE ¹ ² ³ ³ ² ¹ Endothelial cells ¹ ² ³ Percentage C Purity D Recovery Purity Recovery C Figure 5: Cardiac fibroblasts were isolated from dissociated neonatal mouse and rat hearts (P P3). Purities of up to 96% were obtained using the Neonatal Cardiac Fibroblast Isolation Kit (). Immunofluorescent stainings of mouse cells were performed before () and after (C) isolation. Cells were stained for α-actinin (green) and vimentin (red). The blue stain (DPI) denotes the nuclei. Figure 6: Cardiac endothelial cells were isolated from dissociated neonatal mouse hearts (P P3) using the Neonatal Cardiac Endothelial Cell Isolation Kit, mouse (). The use of the Neonatal Cardiac Endothelial Cell Isoaltion Kits, mouse or rat results in high yields of viable endothelial cells with purities of up to 97% (). Isolated mouse endothelial cells showed their characteristic morphology in cell culture (C). Moreover, the functionality of enriched mouse endothelial cells was proven by the endocytosis of Dil-c-LDL (D).

5 Product list Product Description Order no. Sample preparation gentlemcs Dissociator gentlemcs Octo Dissociator gentlemcs Octo Dissociator with Heaters Neonatal Heart Dissociation Kit, mouse and rat Embryoid ody Dissociation Kit, human and mouse C Tubes, Content: 25 tubes C Tubes, Content: tubes enchtop instrument for the automated dissociation and homogenization of tissues. enchtop instrument for the automated dissociation or homogenization of up to eight tissue samples. enchtop instrument with integrated heating function for the automated dissociation or homogenization of up to eight tissue samples. Gentle and effective dissociation of mouse and rat neonatal hearts into single-cell suspensions. For 25 preparations. Preparation of single-cell suspensions from embryoid bodies. For 5 preparations. gentlemcs Tubes for the dissociation of tissues to obtain single-cell suspensions. gentlemcs Tubes for the dissociation of tissues to obtain single-cell suspensions M Tubes, Content: 25 tubes gentlemcs Tubes for the homogenization of tissues M Tubes, Content: tubes gentlemcs Tubes for the homogenization of tissues MCS Tissue Storage Solution Solution for tissue storage and shipping. 3-8 Cell separation Neonatal Cardiac Endothelial Cell Isolation Kit, mouse Kit for the isolation of neonatal endothelial cells from mouse heart Neonatal Cardiac Endothelial Cell Isolation Kit, rat Kit for the isolation of neonatal endothelial cells from rat heart Neonatal Cardiac Fibroblast Isolation Kit, mouse Kit for the isolation of neonatal fibroblasts from mouse heart Neonatal Cardiomyocyte Isolation Kit, mouse Kit for the untouched isolation of neonatal cardiomyocytes from mouse heart Neonatal Cardiac Fibroblast Isolation Kit, rat Kit for the isolation of neonatal fibroblasts from rat heart Neonatal Cardiomyocyte Isolation Kit, rat Cardiac Progenitor Cell Isolation Kit (Sca), mouse Kit for the untouched isolation of neonatal cardiomyocytes from rat heart. Kit for the isolation of cardiac progenitor cells from mouse heart tissue

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6 miltenyibiotec.com/cardiac Germany/ustria/ Switzerland Miltenyi iotec GmbH Friedrich-Ebert-Straße ergisch Gladbach Germany Phone Fax US/Canada Miltenyi iotec Inc. 233 Lindbergh Street uburn, C 9562, US Phone 8 FOR MCS Phone Fax macs@miltenyibiotec.com ustralia Miltenyi iotec ustralia Pty. Ltd. Unit 6, 2 Eden Park Drive Macquarie Park NSW 23 ustralia Phone Fax macs@miltenyibiotec.com.au enelux Miltenyi iotec.v. Schipholweg 68 H 236 XE Leiden The Netherlands macs@miltenyibiotec.nl Customer service The Netherlands Phone Fax 8 42 Customer service elgium Phone Fax Customer service Luxembourg Phone Fax China Miltenyi iotec Technology & Trading (Shanghai) Co., Ltd. Room 239 No. 39, Xianxia Road Changning District 25 Shanghai, P.R. China Phone Fax macs@miltenyibiotec.com.cn France Miltenyi iotec SS rue Mercoeur 75 Paris, France Phone Fax macs@miltenyibiotec.fr Italy Miltenyi iotec S.r.l. Via Persicetana, 2/D 42 Calderara di Reno (O) Italy Phone Fax macs@miltenyibiotec.it Japan Miltenyi iotec K.K. Nittsu-Eitai uilding 5F 6 Fuyuki, Koto-ku, Tokyo 35-4, Japan Phone Fax macs@miltenyibiotec.jp Nordics and altics Miltenyi iotec Norden Scheelevägen Lund Sweden macs@miltenyibiotec.se Customer service Sweden Phone 2 8 Fax Customer service Denmark Phone Fax Customer service Norway, Finland, Iceland, and altic countries Phone Fax Singapore Miltenyi iotec sia Pacific Pte Ltd. each Road #28-6 to 28-8 Shaw Tower Singapore 8972 Phone Fax macs@miltenyibiotec.com.sg Miltenyi iotec provides products and services worldwide. Visit to find your nearest Miltenyi iotec contact. South Korea Miltenyi iotec Korea Petra building 659 Yeoksam-dong Gangnam-gu Seoul, South Korea Phone Fax macs@miltenyibiotec.co.kr Spain Miltenyi iotec S.L. C/Luis uñuel 2 Ciudad de la Imagen Pozuelo de larcón (Madrid) Spain Phone Fax macs@miltenyibiotec.es United Kingdom Miltenyi iotec Ltd. lmac House, Church Lane isley, Surrey GU24 9DR, UK Phone Fax macs@miltenyibiotec.co.uk Unless otherwise specifically indicated, Miltenyi iotec products and services are for research use only and not for therapeutic or diagnostic use. automcs, gentlemcs, and MCS are registered trademarks or trademarks of Miltenyi iotec GmbH. Copyright 25 Miltenyi iotec GmbH. ll rights reserved

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