Degradation Characteristics and Energy Value of Grains, Oil Seed Cakes and Agroindustrial By-Products

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1 Indian Journal of Animal Nutrition Degradation Characteristics and Energy Value of Grains, Oil Seed Cakes and Agroindustrial By-Products Sonali Prusty*, S.S. Kundu, Umesh Sontakke and P.A. Bala Dairy Cattle Nutrition Division, National Dairy Research Institute, Karnal , (Haryana), India ABSTRACT In the present study grains, oil seed cakes and agroindustrial by-products were analyzed for their carbohydrate (CHO) and protein fractions, rumen degradable (RDP) and rumen undegradable protein (RUP) and energy content. Among grains, soluble carbohydrate fraction i.e sugars and organic acids (CA) and soluble available fiber (CB 1 ) was highest in wheat (10.72%, 69.57%) whereas insoluble available fiber (CB 2 ) and unavailable fiber (CC) was highest in barley (27.52%) and pearl millet (7.59%), respectively. In oil seed cakes, CA ranged from (sesame cake) to 49.05% (mustard cake), CB 1 from (SFC) to 28.97% (karanj cake), CB2 from 6.67 (mustard cake) to 37.58% (jatropha cake) and CC from 6.17 (SBC) to 34.66% (CSC), respectively. Among agro-industrial by-products, guar churi had lowest (3.85%) and tamarind seed had highest (36.01%) unavailable carbohydrate. Among grains, non-protein nitrogen (PA) component ranged from 7.83 (maize) to 46.52% (barley) whereas instantly soluble (PB 1 ), readily degradable (PB 2 ), slowly degradable (PB 3 ) and indigestible (PC) proteins ranged from 1.46% (barley) to 16.5% (pearl millet), 26.02% (barley) to 62.98% (maize), 8.53% (pearl millet) to 21.72% (barley) and 3.91% (wheat) to 5.12% (pearl millet), respectively. Oil seed cakes had lower PC (1.07 to 4.2%) fraction. RUP (%CP) was lowest in gram churi (17.12%) and guar korma (18%) and highest in mango seed kernel (44.94%) whereas RDP (%CP) varied from 55.07% to 82.88% amongst the ingredients. The ME value ranged from 1.90 in jatropha cake to 3.16 Mcal/Kg in maize gluten meal (MGM). Keywords: Carbohydrate fractions, Concentrates, Protein fractions, RDP, RUP INTRODUCTION In ruminant animals, rumen microbes utilize different fractions of protein, non-fiber carbohydrate and structural carbohydrate (CHO) at different rates. When rate of protein degradation exceeds the rate of CHO fermentation, large quantity of N is lost as NH 3. On the other hand, when rate of CHO fermentation exceeds protein degradation rate, there is inefficient microbial protein production. Therefore, estimation of CHO and protein fractions in the feedstuffs as well as their degradation rates provides more precise information about the availability of nutrients to rumen microbes and the animal. Cornell net carbohydrate and protein (CNCP) system assumes that feedstuffs are composed of protein, carbohydrate, fat, ash, and water while protein and carbohydrate are further subdivided by chemical composition, physical characteristics, ruminal degradation and post ruminal digestibility characteristics. This system provides the basic *Corresponding author- world.sonalindri@gmail.com structure necessary to predict more accurately the ruminally degraded carbohydrate and nitrogen fractions and absorbed amino acids from microbial and feed protein. Few data are available (Jeya Prakash et al., 2003; Kamble et al., 2010 and Gupta et al., 2011) regarding the CNCP fractions of Indian feeds. So, in the current study carbohydrate and protein fractions and subsequently the rumen degradable (RDP) and undegradable CP (RUP) and energy values of feeds using prediction equations (NRC, 2001) based on their composition were determined. MATERIALS AND METHODS The samples viz. maize, pearl millet, barley, wheat, cotton seed cake (CSC), groundnut cake (GNC), mustard cake-deoiled (DOMC), mustard cake, jatropha cake, karanj cake, sesame cake, soybean meal, sunflower cake, rice polish, rice bran, maize gluten meal (MGM), deoiled rice bran (DORB), guar churi, maize bran, guar korma, gram churi, tamarind seed and 381

2 Prusty et al. mangoseed kernel were collected from local market of Karnal (Haryana), Directorate of Wheat Research (DWR), Karnal and other parts of India, dried in a hot air oven at 60ºC until constant weight, ground to pass through 1mm sieve using electrically operated Willey mill and stored in plastic sample bottles for further analysis. Apart CF and NFE, the proximate (AOAC, 2005) and fiber composition (Van Soest et al. 1991) of feed samples were analyzed. NDF was assayed without α-amylase and expressed exclusive of residual ash. ADF was also expressed exclusive of residual ash. Lignin was estimated by hydrolysis of acid detergent residue in 72% H 2 SO 4 for 3h (Van Soest et al. 1991). Starch was estimated as per the procedure applicable to grains, stock feeds, and cereals given in AOAC (2005). Neutral detergent insoluble protein (NDICP) and acid detergent insoluble protein (ADICP) were estimated by analyzing the CP content of the residual NDF and ADF (Licitra et al. 1996). Phosphate buffer soluble protein (SOLP) was estimated from the CP analysis of the residue obtained after treating 0.5g of sample in borate phosphate buffer. Similarly TCA precipitable protein was analyzed (Licitra et al. 1996). Carbohydrate fractions viz. soluble carbohydrates i.e sugars and organic acids (CA), soluble available fiber (CB 1 ), insoluble available fiber (CB 2 ) and unavailable fiber (CC) and protein fractions viz. non-protein nitrogen (PA), instantly soluble (PB 1 ), readily degradable (PB 2 ), slowly degradable (PB 3 ) and indigestible (PC) were calculated using equations given by Sniffen et al. (1992). Non structural CHO (NSC) was calculated as the difference of the sum of CB 2 and CC from total CHO. TDN and ME were calculated from chemical composition (CP, EE, NDF, NDICP, non fibrous carbohydrate (NFC)) based equations proposed by NRC (2001). The RDP and RUP were analyzed from the protein fractions as per NRC (2001). The fractional degradation rate of the three B fractions are B 1 ( %/h), B 2 (3-16 %/h) and B3 ( %/h) (NRC. 2001). The median value of the given range of Kd is used in the equation 1 and 2 to calculate the RDP and RUP of the feeds whereas the ruminal outflow rate (kp) is assumed to be 0.05h-1 for concentrates. RDP = A + B 1 [kdb 1 / (kdb 1 + kp)] + B 2 [kdb 2 / (kdb 2 + kp)] + B 3 [kdb 3 / (kdb 3 + kp)] (1) RUP = B 1 [kp / (kdb 1 + kp)] + B 2 [kp / (kdb 2 + kp)] + B 3 [kp / (kdb 3 + kp)] + C (2) RESULTS AND DISCUSSION Among grains, the highest OM was found in pearl millet (98.03%) and lowest in maize (97.87%). The CP content was maximum in wheat (15.77%) and minimum in barley (11.63%) whereas EE values ranged from 2.15% (wheat) to 6.25% (maize). CP values of maize, barley and wheat were similar to earlier reported values (Trivedi et al. 2007; Kamble et al. 2010). CP content of oil seed cakes ranged from 18.12% (jatropha cake) to 47.35% (SBM). Proximate composition of CSC, GNC, mustard cake and SBM agrees with the findings of Trivedi et al. (2007). Comparable CP content of GNC, mustard cake, and CSC has been reported earlier (Kamble et al. 2010). Among agroindustrial by-products, CP content was highest in MGM (62.12%) and the lowest in mangoseed kernel (3.75%) whereas the highest EE was recorded in rice bran (16.1%) and the lowest in maize bran (1.72%). Among grains NDICP (%CP) ranged from 13.65% (pearl millet grain) to 26.00% (barley grain) whereas ADICP (%CP) ranged from 3.91% (wheat) to 5.12% (pearl millet). Starch (%NSC) ranged from 74.19% (wheat) to 90.60% (maize). SOLP (%CP) varied from 18.29% (maize grain) to 47.98% (barley grain). Of the total soluble nitrogen (%SOLP) NPN portion was maximum in barley grain (96.96) and minimum in maize grain (42.84). SOLP (%CP), ADICP (%CP), NPN (%SOLP) and NDICP (%CP) in barley and maize grain were comparable to the findings of Sniffen et al. (1992) and starch (%NSC) of barley and maize grain agrees to the findings of Fox et al. (2003). Guevara-Mesa1 et al. (2011) reported similar fiber bound CP of SBM to present finding. MGM, maize, pearl millet, GNC and guar churi were highly energy rich with ME value exceeding 3 Mcal/Kg and TDN ranging from 77-80%. Jatropha (1.90 Mcal/Kg) and sesame cake (1.94 Mcal/Kg) had lowest ME value. Suksombat et al (2007) reported more or 382

3 Degradation characteristics and energy value of different feeds Table1. Chemical composition, fiber and protein fractions and energy value of feeds (% DM basis) Feed NDF ADF Lignin Starch, CP SOLP, NPN, NDICP, EE Ash TDN ME, %NSC %CP %SOLP %CP 1x Mcal/ kg Maize Pearl millet Barley Wheat Cotton seed cake Groundnut cake Mustard cake-deoiled Mustard cake Jatropha cake Karanj cake Sesame cake Soybean meal Sunflower cake Rice polish Rice bran Maize gluten meal Deoiled rice bran Guar churi Maize bran Guar korma Gram chuni Tamarind seed Mangoseed kernel

4 Prusty et al. less similar TDN1X value of soybean meal and rice bran present findings. Among grains, the highest CA and CB 1 fraction was observed in wheat (10.72% and 69.57%, respectively) whereas the lowest CA fraction was found in maize (4.08%) and that of CB 1 in barley grain (57.89%) (table 2). Barley had the highest (27.52%) and wheat had lowest (12.79 %) CB 2. Pearl millet and barley grain had the highest (7.59%) and lowest (6.22%) values for CC, respectively. Due to less CC fraction (<8% of total carbohydrate), most of the CHO derived from grains are digestible in the animal. Maize grain contained negligible amount of fraction C (0.6%) (Chen et al., 1999). Among energy sources highest CHO has been reported in barley grain by Trivedi et al. (2007). Almost similar pattern for CHO fractions of oil seed cakes has been reported by Trivedi et al. (2007). Among oil seed cakes, the highest CHO content was present in jatropha cake (71.70%) followed by karanj cake (67.11%) and sesame cake (63.77%). The fraction CA was the highest in mustard cake (49.05%) and lowest in sesame cake (23.68%). The maximum CB 1 fraction was observed in karanj cake (28.97%) and minimum in sunflower cake. (13.63%) while CB 2 level was the highest in jatropha cake (37.58%) and lowest in mustard cake (6.67%). Fraction C ranged from 6.17% (SBM) to 37.69% (sesame cake). Total CHO among agro-industrial by-products was the highest in mangoseed kernel (88.71%) followed by maize bran (87.42%). The highest CA was observed in guar korma (42.27%) and the lowest in maize bran (5.68%). The maximum value of CB1 was of tamarind seed (47.07%) and minimum of MGM (9.46%). CB 2 fraction was the lowest in tamarind seed (4.05%) and highest in mangoseed kernel (57.59 %). Among agro-industrial by-products, fraction CB 1 was highest in rice polish. Among grains, maize had the lowest PA (7.83) and highest PB 2 (62.98%) whereas barley had the highest PA (46.52) and lowest PB 2 (26.02%). Fraction PC was the highest in pearl millet (5.12%) and lowest in wheat (3.91%). The highest PA (46.52) and PB3 (21.72%) was found in barley. PB 1 (16.50%) was the highest in pearl millet. PA + PB 1 ranged from to 23.86% in grains. The PA + PB 1 and PB 2 fractions of grains were comparable to the findings of Sharma and Singh (1997). PB 3 +PC fraction of maize grain was in corroboration with the findings of Lanzas et al. (2007). The protein fractions of barley grain were supported by the findings of Fazaeli et al. (2012). In GNC, PB 2 (14.15%) was observed to be lower compared to other oil seed cakes. The level of PB 2 in mustard cake, sesame cake, DOMC and CSC was found to be 37.65, 42.05, and 63.05%, respectively. The PC fraction was very low (1.07 to 8.61) in oil seed cakes. The protein fractions of oil seed cakes are similar to previous findings (Gupta et al. 2011). The nitrogen fractions of GNC estimated by Singh et al. (2002) were different from the present finding which might be due to difference in variety, processing methods etc. Lower PC fraction reflects the fair availability of nitrogen to the animal from oil seed cakes. The PB fractions (PB 1, PB 2 and PB 3 ) of CSC corroborated with the findings of Guevara-Mesa et al. (2011). RUP (%CP) varied from 17.12% to 44.94% whereas, RDP (%CP) varied from 55.07% to 82.88%. The RDP (%DM) was lowest in mango seed kernel (2.07%) and guar korma (43.46%) and MGM (40.69%) had highest value for it whereas, RUP (%DM) was lowest in mango seed kernel (1.69%) and highest in MGM (21.43%). Similar RDP and RUP of rice bran and soybean meal were reported by Suksombat et al. (2007). CONCLUSION Maize grain, pearl millet had higher ME (3.1 Mcal/ kg) and guar churi and MGM had about 50% bypass protein. Agro industrial byproducts viz. rice polish, rice bran, MGM, DORB, guar churi and guar korma had good proportion of instantly soluble and degradable CHO. Their combination with the oil cakes viz. GNC, mustard cake, karanj cake and sunflower cake having higher PA+PB 1 fraction would result in efficient ration formulation. The grains were rich in CB fraction whereas cakes viz. CSC, GNC, DOMC, jatropha cake, sesame cake and soybean meal were rich in PB (true protein) fraction. 384

5 Degradation characteristics and energy value of different feeds Table2. Carbohydrate and protein fractions of the feeds as per Cornell Net Carbohydrate and Protein System and their rumen degradable and undegradable protein fractions Feed CA, CB 1, CB 2, CC, PA, PB 1, PB 2, PB 3, PC or RDP, RUP, %CHO %CP ADICP, % %DM %CP Maize Pearl millet Barley Wheat Cotton seed cake Groundnut cake Mustard cake-deoiled Mustard cake Jatropha cake Karanj cake Sesame cake Soybean meal Sunflower cake Rice polish Rice bran Maize gluten meal Deoiled rice bran Guar churi Maize bran Guar korma Gram churi Tamarind seed Mangoseed kernel

6 Prusty et al. REFERENCES AOAC, Official Method of Analysis. 18th ed. Association of Official Analytical Chemists. Chapter 9, 20-22, Virginia, USA. Chen, Y.K., Pell, N.A., Larry, E.C. and Schofield, P Rate and extent of digestion of the ethanol soluble and neutral detergent insoluble fraction of corn grain. J. Anim. Sci. 77: Fazaeli, H., Golmohammadi, H.A., Tabatabayee, S.N. and Asghari-Tabrizi, M Productivity and nutritive value of barley green fodder yield in hydroponic System. World Appl. Sci. J. 16: Fox, D.G., Tylutki, T.P., Tedeschi, L.O., Van Amburgh, M.E., Chase, L.E., Pell, A.N., Overton, T.R. and Russell, J.B The net carbohydrate and protein system for evaluating herd nutrition and nutrient excretion. CNCPS version Guevara-Mesa1, A.L., Miranda-Romero, L.A., Ramírez-Bribiesca1, J.E., González-Muñoz, S.S., Crosby-Galvan1, M.M., Hernández-Calva, L.M. and Del Razo-Rodrígue, O.E Protein fractions and in vitro fermentation of protein feeds for ruminants, Trop. Subtrop. Agroecosyst. 14: Gupta, A., Singh, S., Kundu, S.S. and Jha, N Evaluation of tropical feedstuffs for carbohydrate and protein fractions by CNCP system. Indian J. Anim. Sci. 81: Jeya Prakash R., Kundu, S.S. and Das, M.M Carbohydrate and nitrogen fraction of certain feeds and their utilization in growing buffaloes fed on total mixed ration, Indian J. Anim. Sci. 73: Kamble, A.B., Kundu, S.S., Shelke, S.K., Datt, C. and Jha, N Evaluation of concentrate feedstuffs for carbohydrate and protein fractions and in vitro methane production, Indian J. Anim. Nutr. 27: Lanzas, C., Sniffen, C.J., Seo, S., Tedeschi, L.O. and Fox, D.G A revised CNCPS feed carbohydrate fractionation scheme for formulating rations for ruminants. Anim. Feed Sci. Technol. 136: Licitra. G., Harnandez, T.M. and Van Soest, P.J Standardizations of procedures for nitrogen fractionation of ruminant feeds. Anim. Feed Sci. Technol. 57: National Research Council Nutrient Requirements of Dairy Cattle.7th Revised Edition, National Academy Press. Washington DC. Sharma, R. and Singh, B Nitrogen solubility and dietary protein fraction of various feed stuffs, Indian J. Anim. Nutr. 14: Singh, K.K., Das, M.M., Samanta, A.K., Kundu, S.S. and Sharma, S.D Evaluation of certain feed resources for carbohydrate and protein fractions and in situ digestion characteristics. Indian J. Anim. Sci. 72: Sniffen, C.J., O Connor, J.D., Van Soest, P.J., Fox, D.G. and Russel, J.B A net carbohydrate and protein system for evaluating cattle diets II carbohydrate and protein availability. J. Anim. Sci. 70: Suksombat, W., Lounglawan, P. and Noosen, P Energy and protein evaluation of five feedstuffs used in diet in which cassava pulp as main energy source for lactating dairy cows. Suranaree J. Sci. Technol. 14(1): Trivedi S., Mahanta, S.K. and Kundu, S.S Evaluation of protein rich feed resources with different levels of tannin for their in vitro utilization. Range Mgmt. Agroforestry. 28 (2b): Van Soest, P.J., Robertson, J.B. and Lewis, B.A Methods for dietary fiber, neutral detergent fiber and non starch polysaccharides in relation to Animal Nutrition. Carbohydrate methodology, metabolism and nutritional implications in dairy cattle. J. Dairy Sci. 74: Received on and Accepted on