Phases Available Description Applications Additional Notes RCM-Monosaccharide (L19 packing)*

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1 Carbohydrate and Organic Acid Analysis Excellent resolution Wide range of selectivities Excellent column-to-column reproducibility Recommended alternative to Bio-Rad, Supelco Supelcogel and Waters Sugar-Pak at cost-effective prices (see page ) columns contain sulfonated styrene-divinylbenzene spheres in and 8 % cross-link forms as well as various ionic forms, including calcium, sodium, hydrogen, potassium, lead, and silver. So all you have to do is select the column with the appropriate selectivity. If you are not completely satisfied with the performance of any column, as compared to a competing product of the same size and phase, simply return the column with your comparative data within days for a FULL REFUND. Find the Column For Your Application Phases Available Description Applications Additional Notes RCM-Monosaccharide (L9 packing)* CALCIUM ionic form Monosaccharides and sugar alcohols from sweeteners and corn and cane sugars Di, tri, and tetra saccharides Our most commonly used column type Easy regeneration with calcium nitrate solutions RHM-Monosaccharide (L packing)* RAM-Carbohydrate RSO-Oligosaccharide RNO-Oligosaccharide RPM-Monosaccharide (L packing)* RNM-Carbohydrate (L8 packing)* ROA-Organic Acid (L packing)* RFQ-Fast Acid RKP-Potassium RCU-USP Sugar Alcohols (L9 packing)* HYDROGEN ionic form SILVER ionic form % cross-linked resin SILVER ionic form % cross-linked resin SODIUM ionic form LEAD ionic form SODIUM ionic form HYDROGEN ionic form HYDROGEN ionic form POTASSIUM ionic form CALCIUM ionic form * United States Pharmacopeia (USP) Monosaccharides in combination with organic acids, fatty acids, alcohols, ketones, neutral compounds, or inorganic salts Selectivity complimentary to other column types High resolution of oligosaccharides up to 8 degrees of polymerization (Dp) High resolution of oligosaccharides Monosaccharides and sugar alcohol analysis. Cellobiose, glucose, xylose, arabinose, and mannose & other cellulose products For matrices which contain high concentration of inorganic sodium, Example-molasses Organic acids alone or in combination with carbohydrates, alcohols, fatty acids, or neutral compounds; Ao sugars Rapid screening of fruit quality; ethanol, acetic acid, glycerol, & standard alcohol mixtures Analysis of glyphosate For sugar analysis according to the USP procedures Versatile column, generally run with a mobile phase of deionized water Guard column is recommended to protect the ionic integrity of the matrix Easily regenerated to the original ionic strength Selectivity can be altered by changing the ph as well as the type of dilute eral acid used as the mobile phase Analytes are routinely chromatographed under utes Sorbitol and mannitol can be resolved using simple isocratic conditions HPLC

2 Bioethanol Fermentation Monitoring Easy quantitation of ethanol fermentation broth components Monitors starches, sugars, organic acids, and ethanol in one run Reliable lactic acid and acetic acid monitoring Increase throughput by reducing run times % with mm column length Monitoring the key reaction components throughout the fermentation process is crucial for maximizing ethanol recovery. ROA is uniquely suited for the separation and analysis of simple and complex sugars, organic acids, and ethanol within a fermentation broth sample. With results easily obtained through an isocratic run, ROA is instrumental in helping you to accurately detere what critical steps need to be taken to ensure the maximum yield is achieved during your fermentation run. ROA has the ability to achieve excellent base line separation between Dp and Dp+, which have proven to be a challenge within the bioethanol industry. It is this great base line separation that affords scientists the opportunity to utilize a shorter column dimension. By using the x.8 mm ROA column, you are able to decrease the run time by % when compared to the average run time on a x.8 mm column. Great Resolution of Dp+ and Dp! Reduce run times by % with mm column length HPLC 8 App ID 8 App ID 8 8 Part No.: H-8-K Mobile Phase:. N Sulfuric C Temperature: C System: Shimadzu Proence LC-A System Sample:. Dp+. Lactic Acid. Dp. Glycerol. Maltose. Acetic Acid. Glucose 8. Ethanol Dimensions: x.8 mm Part No.: F-8-K Mobile Phase:. N Sulfuric C Temperature: C System: Shimadzu Proence LC-A System Sample:. Dp+. Lactic Acid. Dp. Glycerol. Maltose. Acetic Acid. Glucose 8. Ethanol Shorten GC Fuel Quality Testing Zebron ZB-Bioethanol GC column can shorten your quality test down to utes! (See p. 8). Extend Column Lifetime Protect the HPLC column from the intrusion of the metal ions by using Phenex Syringe Filters and SecurityGuard. The filters and SecurityGuard guard cartridge system work by trapping metal ions, such as calcium, magnesium, and iron, which can damage the column and cause it to lose or change separation efficiency. (See pp. and ). 8

3 Organic Acids Part No.: H-8-K Mobile Phase:. N Sulfuric Acid Flow Rate:. ml/ Detection: nm Temperature: C Sample:. Oxalic. Citric. Tartaric. Succinic. Formic. Acetic Saccharides Part No.: H--K Detection: Rl Temperature: 8 C Sample:. Melezitose. Maltose. Glucose. Mannose. Fructose. Ribitol 8 HPLC App ID App ID App ID App ID Apple Juice Part No.: H--K Temperature: C Sample:. Sucrose. Glucose. Fructose Carbohydrates Column: RKP-Potassium Part No.: H--K Flow Rate:. ml/ Temperature: 8 C Sample:. Maltotriose. Maltose. Glucose Oligosaccharides Column: RSO-Oligosaccharide Dimensions: x mm Part No.: P--N Flow Rate:. ml/ Detection: Rl Temperature: C Sample: Malto-Oligosaccharides as shown Dp Saccharides Column: RPM-Monosaccharide Part No.: H--K Temperature: C Sample:. Stachyose. Maltose. Glucose. Xylose. Galactose. Fructose. Meso-Erythritol 8. Mannitol 9. Salicin. Xylitol. Sorbitol Dp 8 Dp Dp 9 Dp Dp9 Dp8 Dp Dp Dp Dp Dp Dp Dp App ID App ID 8 Dp refers to Degree of polymerization 9

4 Fermentation Broth Part No.: H--K Flow Rate:. ml/ Temperature: 8 C Sample:. Sucrose. Glucose. Galactose. Fructose App ID 98, 988, 989 Mannitol and Sorbitol Column: RPM-Monosaccharide Dimensions: x.8 mm Part No.: D--K Temperature: 8 C Sample:. Mannitol. Sorbitol App ID 8 HPLC Carbohydrates Column: RNM-Carbohydrate Part No.: H--K Temperature: C Sample:. Stachyose. Cellobiose. Glucose. Fructose. Arabinose. Ribose. Salicin ( % solution of each) Carboxylic Acids Part No.: H-8-K +. % Trifluoroacetic Acid Flow Rate:. ml/ Temperature: C Sample:. Acetylene Carboxylic Acid. Maleic Acid. Succinic Acid. Fumaric Acid App ID App ID 8 Food Softeners Part No.: H--K Flow Rate:. ml/ Temperature: C Sample:. Glycerol. Methoxypolyethylene Glycol. Triethylene Glycol. Sorbitol. Urea Ao Sugars Part No.: H-8-K Mobile Phase: % Phosphoric Acid Temperature: Ambient Sample:. Glucose. N-Acetylglucosae. N-Acetylgalactosae App ID 8 App ID....

5 vs. Bio-Rad guarantees satisfaction when using HPLC columns. As illustrated below, offers advantages that enhance chromatographic results, increase throughput, and simplify quantitation. Easier, Accurate Quantitation Due to improved peak shape Saccharides HPX-8C Temperature: 8 C Sample:. Melezitose. Maltose. Glucose. Mannose. Fructose. Ribitol Orange Juice HPX-8C Temperature: 8 C Sample:. Sucrose. Glucose. Fructose 8 8 Superior Peak Shape RCM HPX-8C App ID 9 App ID Difficult Quantitation RCM HPX-8C App ID 98 App ID 98 HPLC Longer Column Lifetime Due to lower backpressures Aliphatic Acids HPX-8H Mobile Phase:. N H SO Flow Rate:. ml/ Detection: nm Temperature: C Sample:. Oxalic Acid. Citric Acid. Tartaric Acid. Succinic Acid. Formic Acid. Acetic Acid Baseline Separation of Critical Sample Components Due to improved resolution Sugars HPX-8C Temperature: 8 C Sample:. Sucrose. Glucose. Galactose. Fructose. Sorbitol 8 8 Bar Bar ROA HPX-8H App ID 9 App ID Baseline Resolution RCM HPX-8C App ID 9 App ID 98 Comparative separations may not be representative of all applications.

6 Specifications and Operating Recommendations RCM Monosaccharide RSO Oligosaccharide RNO Oligosaccharide RNM Carbohydrate RAM Carbohydrate Part Number H--K P--N P--N H--K H--K Ionic Form Calcium Silver Sodium Sodium Silver Standard Dimensions x.8 mm x mm x mm x.8 mm x.8 mm Matrix Sulfonated Styrene Divinyl Benzene Cross Linking 8 % % % 8 % 8 % Particle Size 8 μm μm μm 8 μm 8 μm Min. Efficiency (p/m) based,,, on last peak Typical Pressure Max. Pressure,,, Max. Flow Rate (ml/). (see pressure).... Max. Temperature ( C) Typical Mobile Phase Water Water Water Water Water ph Range Neutral Neutral Neutral Neutral Neutral Guard Column Part No. B--K R--N R--N B--K B--K Cleaning, Regeneration and Storage Organic Modifiers (Max) % Methanol, IPA, EtOH, Acetonitrile Inorganic Modifiers % CaSO, Ca(NO ), CaCl % Silver Nitrate % Sodium Salts % Sodium Salts % Silver Nitrate Avoid Acids, Bases, Non-Calcium Salts/ Metal Ions, > % Organic Acids, Bases, Non-Silver Salts/ Metal Ions, > % Organic Acids, Bases, Non-Sodium Salts/ Metal Ions, > % Organic Acids, Bases, Non-Sodium Salts/ Metal Ions, > % Organic Acids, Bases, Non-Silver Salts/ Metal Ions, > % Organic HPLC Cleaning Solvent % Water % Water % Water % Water % Water Flow Rate(mL/)..... Temperature ( C) 8 Duration (hrs) Regeneration Solvent. M Ca(NO ). M AgNO. M NaNO. M NaNO. M AgNO Flow Rate (ml/)..... Temperature ( C) Duration (hrs) Ship/Storage Solvent Water Water Water Water Water RPM Monosaccharide RHM Monosaccharide ROA Organic Acid RFQ Fast Acid RCU Sugar Alcohols Part Number H--K H--K H-8-K D--K G--D Ionic Form Lead Hydrogen Hydrogen Hydrogen Calcium Standard Dimensions x.8 mm x.8 mm x.8 mm x.8 mm x. mm Matrix Sulfonated Styrene Divinyl Benzene Cross Linking 8 % 8 % 8 % 8 % 8 % Particle Size 8 μm 8 μm 8 μm 8 μm 8 μm Min. Efficiency (p/m) based,,, (Acetic Acid),, on last peak Typical Pressure Max. Pressure,,,,, Max. Flow Rate (ml/)..... Max. Temperature ( C) Typical Mobile Phase Water Water. N H SO. N H SO Water ph Range Neutral Neutral Guard Column Part No. B--K B--K B-8-K B--K A--D Cleaning, Regeneration and Storage Organic Modifiers (Max) % Methanol, IPA, EtOH, Acetonitrite Inorganic Modifiers % Lead Nitrate % HNO, H PO % HNO, H PO % HNO, H PO % CaSO, Ca(NO ), CaCl Avoid Acids, Bases, Non-Calcium Acids, Bases, Non-Lead Salts/ Acids, Bases, Salts/ Acids, Bases, Salts, Metal Ions, Acids, Bases, Salts, Metal Ions, Salts, or Metal Ions, Metal Ions, > % Organic Metal Ions, > % Organic ph >, > % Organic ph >, > % Organic > % Organic Cleaning Solvent % Water % Water % Water % Water % Water Flow Rate(mL/)..... Temperature ( C) Duration (hrs) Regeneration Solvent. M Pb(NO ). M H SO. M H SO. M H SO. M Ca(NO ) Flow Rate (ml/)..... Temperature ( C) Duration (hrs) Ship/Storage Solvent Water Water. N H SO. N H SO Water

7 Retention Times for Some Carbohydrates and Sugar Alcohols Counter Ion Analyte RAM Ag + RCM Ca + RNM Na + RHM H + RPM Pb + Adonitol (Ribitol) D-Altrose D-(-)-Arabinose..... D-(+)-Cellobiose D-(+)-Digitoxose Dulcitol..... Meso-Erythritol D-(-)- Fructose..... L-(-)-Fucose D-(+)-Galactose Gentiobiose D-(+)-Glucose Inositol Isomaltose Lactose Lactulose D- Lyxose D- Maltose Maltotriose Maltulose D- Mannitol D-(+)-Mannose Melibiose D-(+)-Melezitose * 9.9 D-(+)-Raffinose *.8 L-(+)-Rhamnose D-(-)-Ribose Salicin D-Sorbitol Stachyose Sucrose *. Trehalose Xylitol D-(+)-Xylose * Partial hydrolysis results. Conditions: (degassed) Temperature: 8 C Column Cross Reference Chart If you are not completely satisfied with the performance of any column, as compared to a competing product of the same size and phase, simply return the column with your comparative data within days for a FULL REFUND. Bio-Rad Supelco Supelcogel Waters Sugar-Pak RCM-Monosaccharide HPX-8C Supelcogel Ca Sugar-Pak -9 RHM-Monosaccharide HPX-8H Supelcogel C-H & H - RPM-Monosaccaride HPX-8P Supelcogel Pb -98 RNM-Carbohydrate HPX-8N - RSO-Oligosaccharide HPX-A Supelcogel Ag & Ag -9 ROA-Organic Acid HPX-8H Supelcogel C-H & H - RFQ-Fast Acid Fast Acid - RKP-Potassium HPX-8K Supelcogel K - RCU-USP Sugar Alcohols Sugar Alcohols -9 HPLC Ordering Information SecurityGuard Analytical Cartridges require universal holder Part No.: KJO-8 Columns Guards SecurityGuard Cartridges (mm) Description Part No. Cross Linkage Ionic Form Size (mm) Price Part No. Size (mm) Price x. /pk RCM-Monosaccharide H--K 8 % Calcium x.8 B--K x.8 AJ-9 RHM-Monosaccharide H--K 8 % Hydrogen x.8 B--K x.8 AJ-9 RAM-Carbohydrate H--K 8 % Silver x.8 AJ-9 RSO-Oligosaccharide P--N % Silver x. R--N x. RNO-Oligosaccharide P--N % Sodium x. R--N x. RPM-Monosaccharide (for USP procedure) H--K D--K 8 % 8 % Lead Lead x.8 x.8 B--K B--K x.8 x.8 AJ-9 AJ-9 RNM-Carbohydrate H--K 8 % Sodium x.8 B--K x.8 ROA-Organic Acid H-8-K 8 % Hydrogen x.8 B-8-K x.8 AJ-9 ROA-Organic Acid F-8-K 8 % Hydrogen x.8 B-8-K x.8 AJ-9 ROA-Organic Acid G-8-E 8 % Hydrogen x. AJ-9 RKP-Potassium H--K 8 % Potassium x.8 RFQ-Fast Acid D--K 8 % Hydrogen x.8 B--K x.8 AJ-9 RCU-USP Sugar Alcohols G--D 8 % Calcium x. A--D x. AJ-9 for ID:.-8. mm For Column Heaters, see p. For our full line of Column Performance Check Standards, see pp. -