HxSil C18, 4.6 x 150mm, 5um (P/N 79868) 1. Oxybenzone 2. Octocrylene 3. Octyl Methoxycinnamate 4. Avobenzone (Parsol 1789) 5.

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1 Page 1 of 5 U S A HPLC Application Index Ordering Information Contact HPLC Support Silica-based columns for general separations: Separation of closely related PAH's Better retention of poorly retained compounds Separation of neutral, acidic and basic compounds without tailing (tailing factor of less than 2.0). Product Features Two particle sizes: 3, 5, 7, 10 and 12-20um. Five column internal diameters: 2.1 to 21.5 mm. Analytical and semiprep/preparative guard columns in 316 stainless steel. What Types of Samples Can HxSil C8, C18 Columns Separate? Sunscreens in Lip Balm 1. Oxybenzone 2. Octocrylene 3. Octyl Methoxycinnamate 4. Avobenzone (Parsol 1789) 5. Octyl Salicylate Conditions: 85:15 Methanol : Water. Injection: 5 µl Detection: UV at 308nm. Sunscreen Compounds 1. 2-Hydroxy-4-methoxybenzophenone 2. 2-Ethylhexyl trans-4-methoxycinnamate 3. 2-Ethylhexylsalicylate Conditions:7:1 Acetonitrile : Water. Injection: 10µL Detection: UV at 254nm. Diphenhydramine in Itch Relief Gel 1. Diphenhydramine 10ppm Conditions:9:1 Methanol : 20mM Potassium Hydrogen Phosphate ph 7.0. Injection: 5µL Detection: UV at 254nm.

2 Page 2 of 5 Diphenhydramine in Itch Relief Gel In Figure 11 the antihistamine diphenhydramine is separated from other components present in the gel in just over 2 minutes. Easy sample preparation is characterized by water dilution, and filtration. The compound of interest (diphenhydramine) is an amine base. Meclizine in Pills 1. Meclizine 1mg/mL Conditions: 9:1 Methanol : 20mM Potassium Hydrogen Phosphate ph 7.0. Isocratic.

3 Page 3 of 5 Meclizine in Motion Sickness Pills The antiemetic, meclizine is separated from excipients in the motion sickness pills. This amine base drug exhibits excellent symmetry (Tailing Factor of 1.061) on a Hamilton HxSil C-18 column. Tetracycline by USP Method HxSil C18, 4.6 x 250mm, 5um (P/N 79869) 1. Tetracycline 0.5mg/mL Conditions: 680mL 0.1N Ammonium Oxalate, 270mL Dimethylformamide, 50mL 0.2M Dibasic Ammonium Phosphate ph 7.6. Injection: 10 µl Detection: UV at 280 nm USP Methods The Hamilton HxSil C-18 column meets the requirements of a USP L1 column and the HxSil C-8 column meets the requirements for an L7 column. The application above illustrates the analysis of tetracycline and the two applications below demonstrate the separation of oxytetracycline and tetracycline using a HxSil C-8 "L7" column and folic acid from methotrexate on a HxSil C-18 "L1" column. Oxytetracycline and Tetracycline HxSil C8, 4.6 x 150mm, 5um (P/N 79102) 1. Oxytetracycline 0.5mg/mL 2. Tetracycline 0.5mg/mL Conditions: 680mL 0.1N Ammonium Oxalate, 270mL Dimethylformamide, 50mL 0.2 M Dibasic Ammonium Phosphate ph 7.6. Injection: 20 ul Detection: UV at 280nm. Folic Acid and Methotrexate by USP Method 1. Folic Acid 100ug/mL 2. Methotrexate 100ug/mL Conditions: 9: M Sodium Phosphate, Dibasic, 0.037M Citric Acid ph 6.0 : Acetonitrile. Isocratic. Flow: 1.2 ml/min. Injection: 10 µl

4 Page 4 of 5 Detection: UV at 302nm. Antibiotics HxSil C8, 4.6 x 150mm (P/N 79102) 1.. Ampicillin 2. Oxacillin 3. Dicloxacillin Conditions: 3:1 0.05M Potassium Phosphate ph 7 : Acetonitrile. Isocratic. Injection: 5µL Small Molecules 1. Uracil 10ppm 2. Caffeine 10ppm 3. Phenol 10ppm 4. Toluene 10ppm 5. Butylbenzene 100ppm 6. Triphenylene 10ppm 7. Amylbenzene 100ppm 8. Terphenyl 10ppm Conditions: 7:3 Acetonitrile : Water. Injection: 50 µl

5 Page 5 of 5 ORDERING INFORMATION HxSil C8 Reversed Phase HPLC Columns C8 C18 I.D. x Length 3 µm 5 µm 3µm 5 µm 2.1 x 33 mm x 50 mm x 75mm x 100mm x 150 mm x 33 mm x 50 mm x 100 mm x 150 mm x 250 mm x 250 mm x 250 mm x 250 mm HxSil C8, C18 Guard Columns C8 C18 Analytical Starter Kits (1 holder, 2 cartridges) Analytical Replacement Cartridges (5/pk) Semiprep/Preparative Starter Kit (1 holder, 1 cartridge) Semiprep/Preparative Replacement Cartridges (2/pk) Guard Column Technical Information Column Care and Storage About Hamilton Contact Quick Order Service News & Promos Copyright Hamilton Company. All Rights Reserved. Terms of Use. Privacy Statement.