Biotronic Top3. Per4izer the breakthrough in animal growth performance. Triple action for improved performance! Naturally ahead
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1 Biotronic Top3 Triple action for improved performance! Per4izer the breakthrough in animal growth performance Naturally ahead
2 CHALLENGES IN ANIMAL PRODUCTION high levels of productivity high-quality products bacterial challenge Salmonella, E. coli, Campylobacter, Clostridia, Streptococcus Feed Water Environment GI tract
3 ANTIBIOTICS VS NATURAL PRODUCTS reduction of common bacterial burden improvement of digestibility kill beneficial microflora no effect on feed hygiene drug-resistant bacteria Natural products as potential alternative to antibiotics
4 ACIDIFICATION Dietary supplementation by acidifiers: reduces ph and buffer capacity reduces pathogens in feed and GIT stimulates growth promotion Feed hygiene and GIT health is improved
5 ENHANCEMENT OF EFFICACY The efficacy of organic acids might be improved Phytochemicals Organic acids combining them with phytochemicals!
6 PHYTOCHEMICAL. DEFINITION A term phytochemical refers to compounds produced by plants which have protective or preventive properties. Phytochemicals are found in fruits, vegetables, beans, grains and other plants. There are more than 1,000 phytochemicals: Anti-oxidant: allyl sulfides, carotenoids, polyphenols Antibacterial: allicin, cinnamaldehyde Hormonal action: isoflavones (soy beans)
7 IMPROVING EFFICACY OF PRODUCTS Individual products Blends of organic acids & phytochemicals Blend of organic acids, phytochemical & permeabilizers
8 PERMEABILIZERS. DEFINITION Permeabilizers weaken the outer membrane (OM) of Gram-negative bacteria facilitate other antimicrobials to enter bacterial cells.
9 BACTERIA STRUCTURE Outer membrane of Gram-negative bacteria contains lipopolysaccharide layer which makes bacteria resistant to: hydrophobic antibiotics, detergents, bile salts, digestive enzymes.
10 BIOTRONIC PRODUCT LINE Biotronic Top3 Active ingredients: Biomin Per4izer Phytochemical Organic acids Sequential Release Medium
11 BLEND OF ORGANIC ACIDS Sequential Release Medium
12 ORGANIC ACIDS. DEFINITION. MODE OF ACTION H + H + dissociated undissociated H + R - RH H + ph reduction Antimicrobial effect
13 ANTIMICROBIAL EFFECT OF ORGANIC ACIDS ph reduction uses energy to restore ph Intestinal ph 5-6 ph 7 Disruption of DNA and protein synthesis Disruption of membranes Bacteria is not able to replicate (Booth, 1985 Stratford & Anslow, Russel & Diez-Gonzales, Roe et al., 1998 Krebs et al., 1983 Cherrington et al., 1983 Freese et al., 1973)
14 ANTIMICROBIAL EFFECT OF INDIVIDUAL ORGANIC ACIDS AGAINST SALMONELLA
15 ANTIMICROBIAL EFFECT OF INDIVIDUAL ORGANIC ACIDS AGAINST E. COLI
16 PHYTOCHEMICAL Sequential Release Medium
17 CINNAMALDEHYDE Cinnamaldehyde is the organic compound that gives cinnamon its flavor and odor. It occurs naturally in the bark of cinnamon trees and other species of the genus Cinnamomum. Cinnamaldehyde plays an essential role in the cell division reducing the proliferation of potentially harmful bacteria.
18 PROKARYOTIC CELL DIVISION Cinnamaldehyde Inhibit FtsZ protein and GTPase activity, and assembly into Z-ring. Bacteria does not divide! Domadia et al., 2007 Nature Reviews. Molecular cell biology FtsZ = filamentation temperature sensitive protein-z GTPase = guanosine 5-triphosphate
19 PER4IZER Sequential Release Medium
20 INNOVATION BIOMIN PER4IZER Biomin Per4izer is the unique permeabilizing substance identified to enhance the antimicrobial effect of the organic acids and the phytochemical against pathogenic bacteria. 1. Per4izer weakens gram-negative bacteria outer membrane 2. Per4izer facilitates entry of organic acids and phytochemical 3. Lethal injury of bacteria
21 IMPROVEMENT OF ANTIMICROBIAL ACTIVITY BY ADDING PER4IZER
22 IN VITRO TRIAL WITH BIOTRONIC TOP
23 CARRIER SEQUENTIAL RELEASE MEDIUM Biomin Per4izer Phytochemical Blend of organic acids Sequential Release Medium
24 CARRIER Vermiculite: phyllosilicate/sheet silicate clean to handle odorless mold resistant. Vermiculite exfoliated expands in a free flowing powder which is able to absorb different substances and serve as carrier.
25 TRIAL RESULTS IN VIVO BW vs NC +7%, vs PC +11% ADWG vs NC +8%, vs PC +16% FCR vs NC -3%, vs PC -1% BW vs NC +7%, ADWG vs NC +8% FCR vs NC -3%
26 EFFECT OF BIOTRONIC TOP3 ON GROWTH PERFORMANCE IN WEANED PIGS Species : weaned piglets (days 28) Duration: 56 d NC = no growth promoter Biotronic Top3 = 1 kg/t Negative control Biotronic Top3 Δ, % No. animals n = 30 n = 30 Initial body weight, kg / Final body weight, kg a b +6 Feed intake, g/animal Daily weight gain, g 481 a 517 b +8 FCR ROI = 11:1 Economical benefit 2.5 /pig a,b Means with different superscripts differ significantly; P<0.05
27 COMMERCIAL TRIAL, CRAILSHEIM, GERMANY Trial design Treatments: 1) Negative control (NC) no AGP or NGP 2) Treatment Biotronic Top3 1 kg/t 90 piglets weaned at 28 days of age/group Duration of the trial: 56 days Parameters: Growth performance (BW, ADWG, FCR)
28 COMMERCIAL TRIAL, CRAILSHEIM, GERMANY Results Body weight (kg), 84 days Average daily gain (g) 4.6% Control group 31.5 Biotronic Top3 group 4.4% Control group 454 Biotronic Top3 group Feed conversion ratio % ROI = 9.8: Control group 1.51 Biotronic Top3 group
29 SCIENTIFIC TRIAL, HUNAN UNI, CHINA Trial design Treatments: 1) Negative control (NC) no AGP or NGP 2) Positive control (PC) Colistin 100g/t + CTC 100g/t 3) Treatment (BTR Top3) Biotronic Top3 1 kg/t Parameters: Growth performance (BW, ADWG, FCR) ph value in feed and GIT Bacteria in GIT Histology of jejunum
30 SCIENTIFIC TRIAL, HUNAN UNI, CHINA Results on growth performance NC PC BTR Top3 No. animals n = 32 n = 32 n = 32 Δ, % NC vs BTR Top3 Δ, % PC vs BTR Top3 Initial BW d35, kg / / Final BW d91, kg 42.5 a 43.2 a 48.1 b FI, g/animal/day 1092 a 1112 a 1269 b DWG, g 580 a 578 ab 668 b FCR Mortality, no of animals 1 2 / a,b Means with different superscripts differ significantly; P<0.05
31 SCIENTIFIC TRIAL, HUNAN UNI, CHINA Results on ph reduction in feed and GIT NC PC BTR Top3 ph in feed Buffer capacity in feed, ml of HCl ph in stomach 4.28 a 4.30 a 3.67 b 0.6 ph in doudenum ph in jejunum ph in ileum ph in colon a,b Means with different superscripts differ significantly; P<0.05
32 SCIENTIFIC TRIAL, HUNAN UNI, CHINA Results on microbes and morphology of GIT Ileum, Log cfu/g NC PC BTR Top3 E. coli a b b Coliforms a b b Salmonella typhimurium a b ab Lactobacillus a a b Bifidobacteria a a b Histology of jejunum Villi length, µm a ab b Crypt depth,µm a,b Means with different superscripts differ significantly; P<0.05
33 CONTRIBUTION TO FEED HYGIENE Agar diffusion test The effect of Biotronic Top3 on the growth inhibition of mold and yeast was evaluated
34 RESULTS Strains Aspergillus fumigatus 1 Fusarium proliferatum Candida famata Candida glabrata Candida krusei Fusarium graminearum Monascus ruber Penicillium roqueforti Inhibition after 96h xxx x xx xx xx xxx xxx x 1 Excellent inhibition after 24h
35 PELLETING TEST Mixer: Bühler Paddelmixer: Mixing accuracy d = Mixing time dry (without fat added): 120 sec* Mixing time in total: 300 sec Pelletiser: Bühler Serial number Type: DPAA Die plate: diameter of the openings: 3.5 mm, length 55 mm Sampling: Samples taken were cooled on plastic plates before packaging Samples were stored at 4 C until further analysis
36 PELLETING TEST Sampling: 3 samples of the pre-mixtures in order to determine mixing accuracy 3 samples from the experimental mixture after the mixing process - without any additional treatment recovery of active ingredients After steam injection (and before the pelletising process) 3 samples were taken and cooled to room temperature before packaging indication of damaged caused by the steam injection (hydro thermal stability) 3 samples of feed pellets were taken and cooled to room temperature before packaging indication of damage caused by steam injection and subsequent pelletising process (hydro thermal-mechanical stability)
37 RECOVERY OF BIOTRONIC TOP3 (90 C) Flavouring agents Organic acids Control Steam heated Pelleted
38 How does Biotronic Top3 work with different antibiotics
39 Inhibition, % CHLORTETRACYCLINE E. coli O55:K59(B5) E. coli O128:H2 Salmonella enteridits Salmonella typhimurium Chlortetracycline Chlortetracycline + Biotronic Top3
40 Inhibition, % CARBADOX E. coli O55:K59(B5) E. coli O128:H2 Salmonella enteridits Salmonella typhimurium Carbadox Carbadox + Biotronic Top3
41 Inhibition, % COLISTIN E. coli O55:K59(B5) E. coli O128:H2 Salmonella enteridits Salmonella typhimurium Colistin Colistin + Biotronic Top3
42 CONCLUSION Biotronic Top3 has no adverse effects on antibiotics Biotronic Top3 has an additive inhibitory effect effect with some antibiotics on some bacterial strains Usage of Biotronic Top3 has no influence on recommended dosage of antibiotics!
43 CONCLUSIONS Dietary supplementation with Biotronic Top3 Improved growth performance Lowered ph and buffer capacity in feed Had an acidification effect in stomach Decreased pathogenic bacteria Spared beneficial bacteria Stimulated growth of villi Contributed to feed hygiene
44 APPLICATION Dose: 1 kg/t of feed for swine Swine: Pre-starter, starter, fattening Weaning diarrhea: for optimum results Biotronic Top3+ a conventional acidifier or probiotics..
45 Thank you for your attention!
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