Approach at lymph node pathology and ancillary techniques
|
|
- Belinda Day
- 5 years ago
- Views:
Transcription
1 Approach at lymph node pathology and ancillary techniques Hans Konrad Müller-Hermelink Institute of Pathology, University of Würzburg Würzburg, Germany
2 1000 km 400 km
3 Würzburg
4 Germany: population Würzburg: population
5
6 .contemporary lymphoma diagnosis integrates histology, immunophenotype, (molecular) genetic data as well as clinical features
7
8
9 Frequency of lymph node diseases in primary material Lymphadenitis % Metastasis % Lymphoma B-cell line: 65% T-cell line: 8% Hodgkin: 27% % Granulomatous lymphadenitis 357 3% Other infectious types of lymphadenitis 230 2% Autoimmune disease 28 <1%
10 Reactive Hyperplasias and Reaction Patterns in different compartments B-cell compartment Follicular hyperplasia Sinusoidal B-cell reaction Marginal zone reaction/ nodules Extrafollicular B-cell activation Plasmacytosis Progressive transformation of germinal centres T-cell compartment Nodular paracortical hyperplasia Diffuse paracortical hyperplasia CD 4 dominant DTH activation CD 8 dominant cytotoxic activation Sinus reactions Sinus histiocytosis Foreign body reaction Mastozytosis Sinus lymphocytosis Macrophage reactions Foreign body reactions Epitheloid cell reactions Purulent granulomas
11 T-zone CD5
12 Diffuse paracortical hyperplasia
13 Diffuse paracortical hyperplasia CD5
14 T-Zone
15 Dermatopathic Lymphadenitis CD1a
16 C CD 8 + cytotoxic hyperplassia in viral lymphadenitis CD 3 CD 8 CD3
17 CD 8 + cytotoxic hyperplassia in viral lymphadenitis Ki67
18 Extrafollicular response Follicular response 9113/01 EFH 15310
19 Naïve B-cell IgM/IgD surface receptor primary follicles mantle zone
20 Mantle zone: IgD FDC: CD23
21 Primary immunoresponse antigen proliferating IgM + cells formation of short-lived plasma cells short-lived plasma cell
22 Primary immunoresponse Brighenti A et al: Histopathology Jul;47(1):90-100
23 Ki-67
24 CD20 CD3
25 CD79a IgLC
26 Secondary immuneresponse antigen Plasma cell protective memory memory cell reactive memory Germinal center reaction Proliferation Affinity maturation by somatic hypermutation Immunglobulin gene class switch >98% of the cells die by apoptosis
27
28 Ki67 BCL2
29 Follicles Medullary cords Extrafoll. activation
30 r CD3 Donkey anti Rabbit m Ki67 Donkey anti Mouse Donkey anti Goat m CD79a Goat anti FITC
31 Germinal center reaction Extrafollicular reaction Bild intrafollicular icsat+ cells are CD79a+ IgL+ super 15310_01 Chef spezial Mantel, Keim, Aussen3_Ov4Ausse n CD79a gruen, Igl rot, Icsat blau.jpg CD79a IgL IRF-4
32 Germinal center reaction Extrafollicular reaction CD79a ICSAT KI67 Ki67 CD79a IRF-4
33 H1317/01 CD79a IgL CD138 germinal center reaction
34 Developmental pathways of the B-cell activation IgM + CD27 CD138 IgG + CD27 + CD138 +
35 antigen antigen plasma cell protective memory memory cell reactive memory short-lived plasma cell
36 B-cell development in lymph nodes plasma cell protective memory Ki67 CD20 CD79a PAX5 PU1 BCL2 CD10 BCL6 IRF-4 BLIMP-1 IgG CD27 CD138 memory cell reactive memory
37 Reactive Hyperplasias and Reaction Patterns in different compartments B-cell compartment Follicular hyperplasia Sinusoidal B-cell reaction Marginal zone reaction/ nodules Extrafollicular B-cell activation Plasmacytosis Progressive transformation of germinal centres T-cell compartment Nodular paracortical hyperplasia Diffuse paracortical hyperplasia CD 4 dominant DTH activation CD 8 dominant cytotoxic activation Sinus reactions Sinus histiocytosis Foreign body reaction Mastocytosis Sinus lymphocytosis Macrophage reactions Foreign body reactions Epitheloid cell reactions Purulent granulomas Plasmacytoid monocyte nodules
38 Sinusoidal B Cell Reaction HE
39 Sinusoidal B Cell Reaction HE
40 Sinusoidal B Cell Reaction Giemsa
41 Sinusoidal B Cell Reaction CD20
42 Sinusoidal B Cell Reaction CD5
43 Sinusoidal B Cell Reaction IRF-4 CD27
44 Sinusoidal B Cell Reaction Ki-67 BCL-2
45 Differenzierungsantigene Keimzentrumsreaktion Plasmazelle Protektives Gedächtnis Ki67 CD20 CD79a PAX5 PU1 BCL2 CD10 BCL6 IRF-4 BLIMP-1 IgG CD27 CD138 Memoryzelle Reaktives Gedächtnis
46 Monocytoid (Sinusoidal) B Cell Reaction Acute prefollicular B cell hyperplasia filling marginal and intermediate sinuses ( different from marginal zone B cell hyperplasia) Acute and subacute Infections: e.g. Toxoplasma gondii HIV EBV CMV Very unusual in malignant lymphoma with the exeption of very rare cases of monocytoid B Cell lymphoma
47 Piringer Lymphadenitis
48 H1645/01 Giemsa x20
49 Reactive Hyperplasias and Reaction Patterns in different compartments B-cell compartment Follicular hyperplasia Sinusoidal B-cell reaction Marginal zone reaction/ nodules Extrafollicular B-cell activation Plasmacytosis Progressive transformation of germinal centres T-cell compartment Nodular paracortical hyperplasia Diffuse paracortical hyperplasia CD 4 dominant DTH activation CD 8 dominant cytotoxic activation Sinus reactions ( medullary) Sinus histiocytosis Foreign body reaction Mastocytosis Sinus lymphocytosis Macrophage reactions Foreign body reactions Epitheloid cell reactions Purulent granulomas Plasmacytoid monocyte nodules
50 Plasmacytoid monocytes Cytokine producing monocyte/dendritic cell population ( IFN-a) in response to viral challenge Characteristic phenotype (CD68+, CD4+, CD56+/-, CD123+, BDCA2+) Seen in 16% of non-specific lymphadenitis cases, particularly prominent in Kikuchi lymphadenitis Related to CD 4+,CD56+ hematodermic neoplasia)
51 H1170/09 HE x40
52 H1170/09 Giemsa x40
53 H1170/09 CD68 x40
54 H8847/00 HE x40
55 H8847/00 CD68 x40
56 Reactive Hyperplasias and Reaction Patterns in different compartments B-cell compartment Follicular hyperplasia Sinusoidal B-cell reaction Marginal zone reaction/ nodules Extrafollicular B-cell activation Plasmacytosis Progressive transformation of germinal centres T-cell compartment Nodular paracortical hyperplasia Diffuse paracortical hyperplasia CD 4 dominant DTH activation CD 8 dominant cytotoxic activation Sinus reactions Sinus histiocytosis Foreign body reaction Mastozytosis Sinus lymphocytosis Macrophage reactions Foreign body reactions Epitheloid cell reactions Purulent granulomas
57 Human Marginal Zone B Cells IgM+,IgD+,CD27+,(CD21+, CD1c +) Prediversification ( hypermutation of BCR) occuring at early age, polyclonal, no selection on antigen priming, no memory (?) Reaction to TI antigen stimulation, but also to TD antigens ( in humanized SCID mice) Non-cognate interaction with Th cells may induce IgH switch Don t enter follicular reactions No AID ( human splenic marginal zone B cells) Unclear whether seperate naive B cell subset or specialized antigen expanded memory population ( e.g. in GALT) K.Willenbrock et al. Eur. J. Immunol. (2005) 35: St.G.Tangye, K.L.Good : J.Immunol (2007) 179: L.Moens, et al. :J.Immunol. (2008)181: ;D.Tarlington JEM (2008)205: S.Pillai, A. Cariappa:Nature Reviews Immunol (2009) 9: J.-C.Weill,S.Weller, C.-A.Reynaud : Annu.Rev.Immunol. (2009) 27:267-85
58 The human marginal zone contains heterogeneous B cell subpopulations; but man are not mice Fo B memory cell reactive memory plasma cell protective memory? Mz B? short-lived plasma cell
59 Marginal zone Reaction( hyperplasia) Marginal zone B cells are heterogeneous Marginal zone has been defined in the spleen, where the outer extrafollicular response is a prefollicular reaction mostly to TI antigens. Similar reactions and structural findings do exist in lymph nodes Better understanding necessary to define the normal counterparts of nodal and splenic MZBL
60 C3d receptor (CD21)
61 marginal zone
62 CD27 expression in B-Lymphocytes Marker for postfollicular cells Somatic hypermutations only in CD27 + B-cells Immunoglobulin isotype switch in CD27+ cells Immunoglobulin production more efficient Cord blood does not contain CD27+ B-cells Agematsu K: Eur J Immunol 1997;27:2075 Klein U: J Exp Med 1998;188:1679 Weller S: PNAS 2001;98: 1166 Nagumo H: Blood 2002;99:567
63 Successive stem cells and differentiation phases in normal B cell development Bone marrow Lymphoid tissue Bone marrow T, NK CD5 +? pluripotent hämopoetic lymphoid VDJ-rearrangement Stem cell Stem cell mature B cell memory B cell plasma cell germinal center extrafollicular activation 1 Heterogeneity 2 Specificity 3 Effectors 4 Defense Organ
64 Successive stem cells and differentiation phases in normal B cell development Bone marrow Lymphoid tissue Bone marrow T, NK CD5 +? Marginal zone B cell pluripotent hämopoetic lymphoid VDJ-rearrangement Stem cell Stem cell mature B cell memory B cell plasma cell germinal center extrafollicular activation 1 Heterogeneity 2 Specificity 3 Effectors 4 Defense Organ
65 Antigen Antigen Plasma Cell protective memory Memory B cell reactive memory B CLL B CLL Akute Lymphoid Leukemia Mantle Cell- Lymphoma Follicular Lymphoma Marginal Zone- B cell Lymphoma Plasmocytoma MM Diffuse Large B Cell Lymphoma Germinal Center B Cell Type Activated B Cell Type
66 Immunophenotypic analysis Detection of a clonal B- or T- cell population Detection of genetic alteration
67 Frequently used immunophenotypic markers Lineage specific Non lineage specific Tumor marker? B-cell T-cells Aberrant subcellular and tissue distribution? Abnormal constellations? PAX5 CD20 CD19 CD23 CD79a CD10 BCL6 MUM1 CD138 κ/λ IgM IgG IgD IgA CD3 CD2 CD5 CD7 CD4 CD8 TdT PD1 Perforin bf1 TdT CD30 Ki67 ALK1 CD56 CD15 BCL2 CyclinD1 EMA
68 Immunophenotypic analysis Flow cytometric analysis of the cell suspension Immunohistochemistry of the tissue section Peripheral blood, bone marrow, body fluids Flexibility Accurate quantitative analysis Simultaneous detection of several markers Topographic distribution of immunostained cell population Routine paraffin section Specimens with a small percentage of tumor cells (HD)
69 Chronic lymphocytic leukaemia CD20 Ki-67 CD5 CD23
70 Mantle cell lymphoma H&E Cyclin D1 CD5 CD23
71 Follicular lymphoma Ki67 BCL2
72 CD20 AE1/AE3
73 Anaplastic large cell lymphoma CD30 ALK1
74 NLPHL Classical HL CD30
75 NLPHL Classical HL CD20
76 A minimum of Immunehistochemistry for lymphoma classification?
77 CD20 / CD5 Distribution and pattern of B and T cell areas Basic structure of the lymph node IgD Follicle Parafollicular pulp Coexpression of CD5 in CLL and Mantle cell lymphoma Mantle zone Expression in Mantle cell lymphoma CD23 Follicular dendritic cells Marginal zone (inconsistent) Coexpression of CD23 in CLL
78 Ki-67 where the music plays Abnormal activities BCL-2 High proliferation in extrafollicular areas Low proliferation within the follicles Expression in all B cells outside of germinal centers Follicular expression in follikular lymphoma Does not distinguisish different types of indolent B cell lymphoma
79 CD30 Hodgkin lymphoma Anaplastic large cell lymphoma Weak expression also in plasma cells (intern control) CD15 Expression in granulocytes Hodgkin lymphoma
80 Immunehistochemistry reactive l.n. CD5 CD20 CD23 Ki-67 CD30 confirmation T cells B cells FDC(marginal zone) Follicle Variabel CLL % MCL + + (FDC remnants) B blasts Richter Syndrom e -40% - FL - + Follicles - - DLBCL (+) (Cyclin D1, p27, IgD) bcl-2 (CD10/ bcl-6) chl rosettes -/+ - Variabel + CD15 PTCL +/- - FDC (AILT) Variabel -/+?
81 Cave! IHC confirms or rejects diagnoses favoured by morphology CD20 CD5 + CD IgD + Ki67 <20% Ki67 variabel Cyclin D1+ B-CLL MCL Ki67 Ki67 >99% Bcl2 CD10+ Burkitt CD ALCL Hodgkin DLBCL CD23 FDC- FDC Ki % BCL2+ CD10+ Ki67 variabel DLBCL FL T cell lymphoma Recurrance after RituxiMab Extranodal Exclude follicular colonisation MZL
82 Immunehistochemistry CD79a Recurrences after Rituximab treatment Later differentiation antigen of B cells CD3 Comparison with CD5 e.g coexpression im B cells IgD L.n. architecture, mantle zone, NLPHL k/l Clonality, rarely characterizes lymphoma type Cyclin D1 Note intern control= endothelia CD10/ BCL-6 follicles BCL-2 Expression in all indolent B cell lymphoma Over expression in follicles of FL LMP-1 EBV in mononucleosis, Hodgkin lymphoma, PTLD etc. CD138 Plasma cells
83 Molecular studies Important help in the diagnosis of malignant lymphoma Morphological approach essential : no tumor diagnosis on molecular findings alone!!!! Diagnostic approach is correlated to treatment options ( e.g. CD20 and RituxiMab treatment)
84 Molecular studies B-cell clonality T-cell clonality IgH PCR Microdissection and IgH PCR Quantitative RT-PCR for kappa and lambda light chains Southern blot TCR PCR a.o. Southern blot Cyclin D1 overexpression Quantitative PCR Mutation analysis Infectious agents Chromosomal Translocations sequencing ( e.g. c-kit, p53, IgH) PCR ( e.g.mycobacteria, Chlamydia) Southern blot, PCR, RT-PCR, FISH
85 The Immunglobulin Receptor a fingerprint of individual clonal development and current activities of B-lymphocytes Clonality and clonal relationship Somatic hypermutation Ongoing mutations VH-family usage Antigen selection Minimal residual disease Idiotypic therapeutic targets
86 Analysis of Somatic Hypermutation of IgVH Genes in B-Cell Lymphoma Lymphoma Somatic Mutation Ongoing Mutations B-CLL + (50%) - B-PLL +??? MCL -/+ - FL MZBL, MALT ++ + MZBL, splenic ++/- - MZBL, nodal ++/- - HCL ++ - Plasmacytoma ++ -
87 Recurrent chromosomal translocations are very frequent in mature B-cell lymphoma Chromosomal translocation Gene function B-NHL subcategory frequency t(14;18)(q32;q21) and variants BCL2 anti-apoptotic protein FL DLBCL CLL 80-90% 20-30% <1% t(1;22)(q22;q11) FCGR2B low affinity Ig Fc receptor FL, DLBCL <1% t(8;14)(q24;q32) and variants MYC transcription factor BL DLBCL FL MM ~100% 5-10% <1% t(3;14)(q27;q32) and variants BCL6 transcriptional repressor DLBCL FL 40%?% t(11;14)(q13;q32) CCND1 G1 cyclin MCL MM >95% ~20% t(9;14)(p13;q32) PAX5 transcription factor variant SLL not established t(11;18)(q21;q21) API2/ MALT1 anti-apoptosis protein MALT ~30%* t(14;15)(q32;q11-13) BCL8 DLBCL ~4% t(1;14)(q21;q32) MUC1 cell surface receptors DLBCL <1% t(1;14)(p22;q32) BCL10 apoptosis regulatory protein MALT t(14;18)(q32;q21) MALT1 MALT ~20%* t(14;19)(q32;q13) BCL3 IκB C L L / S L L < 5 % t ( 4 ; 1 4 ) ( p 1 6 ; q 3 2 ) F G F R 3 / M M S E T M M % t ( 1 4 ; 1 6 ) ( q 3 2 ; q 2 3 ) c - m a f t r a n s c r i p t i o n f a c t o r M M % a n d v a r i a n t t ( 6 ; 1 4 ) ( p 2 1 ; q 3 2 ) C y c l i n D 3 C e l l c y c l e p r o t e i n M M 3-4 % t ( 6 ; 1 4 ) ( p 2 5 ; q 3 2 ) M U M 1 / I R F - 4 i n t e r f e r o n r e g u l a t o r y f a c t o r M M n o t e s t a b l i s h e d d e r 7 q 2 1 C D K 6 c e l l c y c l e k i n a s e s p l e n i c M Z L %
88 t(14;18) t(11;14) t(8:14)/ebl t(3;14)? t(8:14)/sbl t(4;14) t(11;14) Somatic Hypermutation Germinal Center Class Switching V(D)J recombination FDC T Plasma cell Naive B cell apoptosis apoptosis Memory B cell ALL MCL B-CLL(subtype) BL FL DLBCL (subset) MZL B-CLL (subset) DLBCL (subset) MM HCL? secondary genetic alterations: genomic gains,amplifications, deletions, mutations
89 Mantle cell lymphoma Cyclin D1 deregulation
90 Interphase Fluorescence In Situ Hybridization
91 Fluorescence in situ hybridization Relatively simple technique for targeted detection of genetic aberrations Non dividing or terminally differentiated cells, non vital cells, when dividing cells do not represent the malignant clone Provides information on single cell level
92 Commercially available probes
93 FISH on routinely processed paraffin tissue samples not restricted on the availability of fresh or frozen material
94 FISH on routinely processed paraffin tissue samples Nuclei preparation Paraffin sections Avoid cutting and overlapping artifacts easy for evaluation numerical aberrations, deletions, amplification complex patterns detection of the breakpoint in the histological context needs less patients material control on the presence of the tumor population in the sample
95 FISH patterns on routine paraffin tissue sections Intact nuclei 3-4µm Tissue section A B C B D E F G H Normal tissue Tumor tissue
96
97 FISH on paraffin sections - signal patterns
98 Cut off values for different Vysis probes on routine paraffin section Reactive samples Bcl2/IgH (%cells with aberrant pattern) MYC BAP (%cells with aberrant pattern) MALT1 BAP (%cells with aberrant pattern) IGH BAP (%cells with aberrant pattern) mean 11 0,5 4 9,4 SD 2 0,95 1,58 3,4 Cut off Bcl1/IgH (%cells with aberrant pattern)
99 FICTION combined ICH and FISH
100
101 CyclinD1
102 kappa
103 FICTION Kappa/CyclinD1 BAP Kappa/CyclinD1 Double immunofluorescence
104 How to integrate the FISH data into histopathology diagnosis? Genomic abnormality = malignancy Genomic abnormality = lymphoma type FL t(14;18)(q32;q21) + (85%) bcl2 protein + FL t(14;18)(q32;q21) bcl2 protein +/- DLBCL t(14;18)(q32;q21) + GCB expression profile Bcl2 +/- favorable prognosis t(14;18)(q32;q21) bcl2 protein + ABC expression profile poor prognosis
105 Interphase FISH analysis is preferable method for detection of the chromosomal translocations as many breakpoints are dispersed over the large genomic area Both practical and biological considerations are in favor of FISH segregation assay (BAP) in which two differently labeled probes segregate upon a break Introduction of reliable hybridization protocols for paraffin tissue allows the usage of FISH in routine pathology settings Things should be made as simple as possible, but not any simpler. Albert Einstein
106
Immunopathology of Lymphoma
Immunopathology of Lymphoma Noraidah Masir MBBCh, M.Med (Pathology), D.Phil. Department of Pathology Faculty of Medicine Universiti Kebangsaan Malaysia Lymphoma classification has been challenging to pathologists.
More informationMolecular Pathology of Lymphoma (Part 1) Rex K.H. Au-Yeung Department of Pathology, HKU
Molecular Pathology of Lymphoma (Part 1) Rex K.H. Au-Yeung Department of Pathology, HKU Lecture outline Time 10:00 11:00 11:15 12:10 12:20 13:15 Content Introduction to lymphoma Review of lymphocyte biology
More informationMethods used to diagnose lymphomas
Institut für Pathologie Institut für Pathologie Methods used to diagnose lymphomas Prof. Dr.Med. Leticia Quintanilla-Fend Molecular techniques NGS histology Cytology AS-PCR Sanger seq. MYC Immunohistochemistry
More informationContents. vii. Preface... Acknowledgments... v xiii
Contents Preface... Acknowledgments... v xiii SECTION I 1. Introduction... 3 Knowledge-Based Diagnosis... 4 Systematic Examination of the Lymph Node... 7 Cell Type Identification... 9 Cell Size and Cellularity...
More informationGENETIC MARKERS IN LYMPHOMA a practical overview. P. Heimann Dpt of Medical Genetics Erasme Hospital - Bordet Institute
GENETIC MARKERS IN LYMPHOMA a practical overview P. Heimann Dpt of Medical Genetics Erasme Hospital - Bordet Institute B and T cell monoclonalities Rearrangement of immunoglobin and TCR genes may help
More informationSmall B-cell (Histologically Low Grade) Lymphoma
Frequency of Lymphoid Neoplasms Small B-cell (Histologically Low Grade) Lymphoma Stephen Hamilton-Dutoit Institute of Pathology Aarhus University Hospital B-cell neoplasms 88% Diffuse large B-cell lymphoma
More informationDifferential diagnosis of hematolymphoid tumors composed of medium-sized cells. Brian Skinnider B.C. Cancer Agency, Vancouver General Hospital
Differential diagnosis of hematolymphoid tumors composed of medium-sized cells Brian Skinnider B.C. Cancer Agency, Vancouver General Hospital Lymphoma classification Lymphoma diagnosis starts with morphologic
More informationImmunohistochemical classification of haematolymphoid tumours. Stephen Hamilton-Dutoit Institute of Pathology Aarhus University Hospital
Immunohistochemical classification of haematolymphoid tumours Stephen Hamilton-Dutoit Institute of Pathology Aarhus University Hospital Malignant lymphoproliferative diseases What are they? Haematolymphoid
More informationFOLLICULARITY in LYMPHOMA
FOLLICULARITY in LYMPHOMA Reactive Follicular Hyperplasia Follicular Hyperplasia irregular follicles Follicular Hyperplasia dark and light zones Light Zone Dark Zone Follicular hyperplasia MIB1 Follicular
More information3/23/2017. Disclosure of Relevant Financial Relationships. Pitfalls in Immunohistochemistry in Hematopathology: CD20 and CD3 Can Let Me Down?!
Pitfalls in Immunohistochemistry in Hematopathology: CD20 and CD3 Can Let Me Down?! Judith A. Ferry Massachusetts General Hospital Disclosure of Relevant Financial Relationships USCAP requires that all
More information7 Omar Abu Reesh. Dr. Ahmad Mansour Dr. Ahmad Mansour
7 Omar Abu Reesh Dr. Ahmad Mansour Dr. Ahmad Mansour -Leukemia: neoplastic leukocytes circulating in the peripheral bloodstream. -Lymphoma: a neoplastic process in the lymph nodes, spleen or other lymphatic
More informationPathology #07. Hussein Al-Sa di. Dr. Sohaib Al-Khatib. Mature B-Cell Neoplasm. 0 P a g e
Pathology #07 Mature B-Cell Neoplasm Hussein Al-Sa di Dr. Sohaib Al-Khatib 0 P a g e Thursday 18/2/2016 Our lecture today (with the next 2 lectures) will be about lymphoid tumors This is a little bit long
More informationMany of the hematolymphoid disorders are derived
REVIEW ARTICLE Practical Immunohistochemistry in Hematopathology: A Review of Useful Antibodies for Diagnosis Ji Lu, MD and Karen L. Chang, MD Abstract: This review article offers some useful panels of
More information11/8/2018 DISCLOSURES. I have NO Conflicts of Interest to Disclose. UTILTY OF DETECTING PATTERNS
Bharat N. Nathwani, M.D. City of Hope Medical Center Professor, Director of Pathology Consultation Services, 1500 East Duarte Road, Duarte, California, 91010 DISCLOSURES -------------------------------------------------------
More informationAggressive B-cell Lymphomas Updated WHO classification Elias Campo
Aggressive B-cell Lymphomas Updated WHO classification Elias Campo Hospital Clinic, University of Barcelona Diffuse Large B-cell Lymphoma A Heterogeneous Category Subtypes with differing: Histology and
More informationPearls and pitfalls in interpretation of lymphoid lesions in needle biopsies
Pearls and pitfalls in interpretation of lymphoid lesions in needle biopsies Megan S. Lim MD PhD University of Pennsylvania October 8, 2018 Objectives To understand how the trend toward less invasive lymph
More informationNon-Hodgkin s Lymphomas Version
NCCN Clinical Practice Guidelines in Oncology (NCCN Guidelines ) Non-Hodgkin s Lymphomas Version 2.2015 NCCN.org Continue Use of Immunophenotyping/ Genetic Testing in Differential Diagnosis of Mature B-Cell
More informationClassification! Immunohistochemical classification of haematolymphoid tumours. Malignant lymphoproliferative diseases
Immunohistochemical classification of haematolymphoid tumours Haematolymphoid Neoplasias: Leukaemia vs Lymphoma C L O N A L M A L I G N A N C I E S Stephen Hamilton-Dutoit Institute of Pathology Aarhus
More informationA Practical Guide To Diagnose B-Cell Lymphomas on FNAs. Nancy P. Caraway, M.D.
A Practical Guide To Diagnose B-Cell Lymphomas on FNAs Nancy P. Caraway, M.D. Major Factors Impacting Dx Lymphomas on Small Bxs Classification systems Immunophenotyping by multiprobe flow cytometry and
More informationThe development of clonality testing for lymphomas in the Bristol Genetics Laboratory. Dr Paula Waits Bristol Genetics Laboratory
The development of clonality testing for lymphomas in the Bristol Genetics Laboratory Dr Paula Waits Bristol Genetics Laboratory Introduction The majority of lymphoid malignancies belong to the B cell
More informationNon-Hodgkin lymphomas (NHLs) Hodgkin lymphoma )HL)
Non-Hodgkin lymphomas (NHLs) Hodgkin lymphoma )HL) Lymphoid Neoplasms: 1- non-hodgkin lymphomas (NHLs) 2- Hodgkin lymphoma 3- plasma cell neoplasms Non-Hodgkin lymphomas (NHLs) Acute Lymphoblastic Leukemia/Lymphoma
More informationLymphoma Update: Lymphoma Update: What s Likely to be New in the New WHO. Patrick Treseler, MD, PhD University of California San Francisco
Lymphoma Update: What s Likely to be New in the New WHO Blood 127:2375; 2016 Patrick Treseler, MD, PhD University of California San Francisco Lymphoma Update: What IS New in the New WHO! Patrick Treseler,
More informationPhenoPath. Diagnoses you can count on B CELL NON-HODGKIN LYMPHOMA
PhenoPath Diagnoses you can count on B CELL NON-HODGKIN LYMPHOMA C urrent diagnosis of B cell non-hodgkin lymphoma (B-NHL) is based on the 2008 WHO Classification of Tumours of Haematopoietic and Lymphoid
More information88-year-old Female with Lymphadenopathy. Faizi Ali, MD
88-year-old Female with Lymphadenopathy Faizi Ali, MD Clinical History A 88-year-old caucasian female presented to our hospital with the complaints of nausea, vomiting,diarrhea, shortness of breath and
More informationHepatic Lymphoma Diagnosis An Algorithmic Approach
Hepatic Lymphoma Diagnosis An Algorithmic Approach Ryan M. Gill, M.D., Ph.D. University of California, San Francisco PLEASE TURN OFF YOUR CELL PHONES Disclosure of Relevant Financial Relationships USCAP
More informationPathology of the indolent B-cell lymphomas Elias Campo
Pathology of the indolent B-cell lymphomas Elias Campo Hospital Clinic, University of Barcelona Small B-cell lymphomas Antigen selection NAIVE -B LYMPHOCYTE MEMORY B-CELL MCL FL LPL MZL CLL Small cell
More informationChronic Lymphocytic Leukemia Mantle Cell Lymphoma Elias Campo
Chronic Lymphocytic Leukemia Mantle Cell Lymphoma Elias Campo Hospital Clinic, University of Barcelona Small B-cell lymphomas NAIVE -B LYMPHOCYTE MEMORY CELL CLL MCL FL MZL Small cell size Low proliferation
More information5003 Immunohistochemistry in hematopathology, what's in, what's out, what's useful
www.ascp.org/ascp2014 5003 Immunohistochemistry in hematopathology, what's in, what's out, what's useful Kathryn Rizzo, DO, PhD VIRGINIA COMMONWEALTH UNIVERSITY Department of Pathology School of Medicine
More informationThe spectrum of flow cytometry of the bone marrow
The spectrum of flow cytometry of the bone marrow Anna Porwit Lund University Faculty of Medicine Dept. of Clinical Sciences Div. Oncology and Pathology anna.porwit@med.lu.se Disclosure of speaker s interests
More informationDETERMINATION OF A LYMPHOID PROCESS
Chapter 2 Applications of Touch Preparation Cytology to Intraoperative Consultations: Lymph Nodes and Extranodal Tissues for Evaluation of Hematolymphoid Disorders INTRODUCTION As discussed in Chap. 1,
More informationLymphoma/CLL 101: Know your Subtype. Dr. David Macdonald Hematologist, The Ottawa Hospital
Lymphoma/CLL 101: Know your Subtype Dr. David Macdonald Hematologist, The Ottawa Hospital Function of the Lymph System Lymph Node Lymphocytes B-cells develop in the bone marrow and influence the immune
More informationThe next lymphoma classification Luca Mazzucchelli Istituto cantonale di patologia, Locarno
Evolution of classification The next classification Luca Mazzucchelli Istituto cantonale di patologia, Locarno The Lymphoma Forum of Excellence, Bellinzona, January 2011 Rappaport Lukes and Collins (immunophenotype)
More informationHEMATOPATHOLOGY DIAGNOSIS & SUBTYPING. Use of IHC. Use of Polymerase Chain Reaction (PCR) Use of Flow Cytometry
HEMATOPATHOLOGY DIAGNOSIS & SUBTYPING HEMATOPATHOLOGY DIAGNOSIS & SUBTYPING The 2008 WHO classification system for tumors of hematopoietic and lymphoid tissues specifies that various combinations of immunophenotypic
More informationThe Development of Lymphocytes: B Cell Development in the Bone Marrow & Peripheral Lymphoid Tissue Deborah A. Lebman, Ph.D.
The Development of Lymphocytes: B Cell Development in the Bone Marrow & Peripheral Lymphoid Tissue Deborah A. Lebman, Ph.D. OBJECTIVES 1. To understand how ordered Ig gene rearrangements lead to the development
More informationFollicular Lymphoma. ced3 APOPTOSIS. *In the nematode Caenorhabditis elegans 131 of the organism's 1031 cells die during development.
Harvard-MIT Division of Health Sciences and Technology HST.176: Cellular and Molecular Immunology Course Director: Dr. Shiv Pillai Follicular Lymphoma 1. Characterized by t(14:18) translocation 2. Ig heavy
More informationApproach to Core Biopsy Specimens
BDIAP 108th Symposium on Haematopathology Joint Meeting of the BDIAP and BLPG at-bristol, Anchor Road, Harbourside, Bristol BS1 5DB 15th - 17th May 2014 Approach to Core Biopsy Specimens Dr Stefan Dojcinov
More informationFrom Morphology to Molecular Pathology: A Practical Approach for Cytopathologists Part 1-Cytomorphology. Songlin Zhang, MD, PhD LSUHSC-Shreveport
From Morphology to Molecular Pathology: A Practical Approach for Cytopathologists Part 1-Cytomorphology Songlin Zhang, MD, PhD LSUHSC-Shreveport I have no Conflict of Interest. FNA on Lymphoproliferative
More informationExploring the Borderlands between Diffuse Large B-cell Lymphoma and Classical Hodgkin s Lymphoma
Exploring the Borderlands between Diffuse Large B-cell Lymphoma and Classical Hodgkin s Lymphoma Elaine S. Jaffe National Cancer Institute Bethesda, MD, USA On the Pathological Changes In Hodgkin s Disease
More informationAntigen-Independent B-Cell Development Bone Marrow
Antigen-Independent B-Cell Development Bone Marrow 1. DNA rearrangements establish the primary repertoire, creating diversity 2. Allelic exclusion ensures that each clone expresses a single antibody on
More informationDiagnosis of lymphoid neoplasms has been
Iranian Journal of Pathology (2007)2 (1), 1-61 Review Article Mehdi Nassiri Dep. of Pathology, University of Miami Miller School of Medicine, Miami, USA Abstract Correct diagnosis and classification of
More informationImmunobiology 7. The Humoral Immune Response
Janeway Murphy Travers Walport Immunobiology 7 Chapter 9 The Humoral Immune Response Copyright Garland Science 2008 Tim Worbs Institute of Immunology Hannover Medical School 1 The course of a typical antibody
More informationLow-grade B-cell lymphoma
Low-grade B-cell lymphoma Patho-Basic 11. September 2018 Stephan Dirnhofer Pathology Outline Definition LPL, MBL/CLL/SLL, MCL FL Subtypes & variants Diagnosis including Grading Transformation Summary Be
More informationLymphoma: What You Need to Know. Richard van der Jagt MD, FRCPC
Lymphoma: What You Need to Know Richard van der Jagt MD, FRCPC Overview Concepts, classification, biology Epidemiology Clinical presentation Diagnosis Staging Three important types of lymphoma Conceptualizing
More informationEQA SCHEME CIRCULATION 33 EDUCATIONAL SLIDES DR GRAEME SMITH MONKLANDS DGH
EQA SCHEME CIRCULATION 33 EDUCATIONAL SLIDES DR GRAEME SMITH MONKLANDS DGH CASE E1 M: 68 yrs Left destructive sinonasal lesion.?lymphoma?adenocarcinoma CD20 CD10 BCL6 MIB1 Answers Diffuse large B cell
More informationClassification of Hematologic Malignancies. Patricia Aoun MD MPH
Classification of Hematologic Malignancies Patricia Aoun MD MPH Objectives Know the basic principles of the current classification system for hematopoietic and lymphoid malignancies Understand the differences
More informationT cell lymphoma diagnostics and differential diagnosis to Hodgkin lymphoma
T cell lymphoma diagnostics and differential diagnosis to Hodgkin lymphoma Sylvia Hartmann Dr. Senckenberg Institute of Pathology Goethe University Frankfurt Overview Borderline ALCL classical HL Borderline
More informationCase 3. Ann T. Moriarty,MD
Case 3 Ann T. Moriarty,MD Case 3 59 year old male with asymptomatic cervical lymphadenopathy. These images are from a fine needle biopsy of a left cervical lymph node. Image 1 Papanicolaou Stained smear,100x.
More informationUse of MYC, BCL2 and BCL6 FISH for investigations of high grade B cell lymphoma
Use of MYC, BCL2 and BCL6 FISH for investigations of high grade B cell lymphoma Dr Anthony Bench Haematopathology and Oncology Diagnostic Service Cambrıdge Unıversıty Hospitals NHS Foundatıon Trust Cambridge
More informationLymphoid Neoplasms. Sylvie Freeman Department of Clinical Immunology, University of Birmingham
Lymphoid Neoplasms Sylvie Freeman Department of Clinical Immunology, University of Birmingham Incidence of Haematological Malignancies UK2001 (CRUK) Malignancy New Cases All Cancers 271,000 Leukaemia 6,760
More informationMECHANISMS OF B-CELL LYMPHOMA PATHOGENESIS
MECHANISMS OF B-CELL LYMPHOMA PATHOGENESIS Ralf Küppers Abstract Chromosomal translocations involving the immunoglobulin loci are a hallmark of many types of B-cell. Other factors, however, also have important
More informationAggressive B-Cell Lymphomas
Aggressive B-cell Lymphomas Aggressive B-Cell Lymphomas Stephen Hamilton Dutoit Institute of Pathology Aarhus Kommunehospital B-lymphoblastic lymphoma Diffuse large cell lymphoma, NOS T-cell / histiocyte-rich;
More informationCorrigenda. WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues (revised 4th edition): corrections made in second print run
Corrigenda WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues (revised 4th edition): corrections made in second print run In addition to corrections of minor typographical errors, corrections
More informationPrepared by: Dr.Mansour Al-Yazji
C L L CLL Prepared by: Abd El-Hakeem Abd El-Rahman Abu Naser Ahmed Khamis Abu Warda Ahmed Mohammed Abu Ghaben Bassel Ziad Abu Warda Nedal Mostafa El-Nahhal Dr.Mansour Al-Yazji LEUKEMIA Leukemia is a form
More informationIncidence. Bimodal age incidence 15-40, >55 years Childhood form (0-14) more common in developing countries M:F=1.5:1; in all subtypes except NS
Hodgkin Lymphoma Hodgkin Lymphoma 30% of all lymphomas Absolute incidence unchanged Arise in lymph node, cervical region Neoplastic tissues usually contain a small number of tumor cells Incidence Bimodal
More informationAggressive B-cell Lymphoma 2013
Aggressive B-cell Lymphoma 2013 Diffuse Large B-Cell Lymphoma Burkitt Lymphoblastic lymphoma Gray zone Intermediate DLBCL/HL Intermediate BL/DLBCL Diffuse Large B-cell lymphoma Common morphology: diffuse
More informationHIGH GRADE B-CELL LYMPHOMA DAVID NOLTE, MD (PGY-2) HUSSAM AL-KATEB, PHD, FACMG DEBORAH FUCHS, MD
HIGH GRADE B-CELL LYMPHOMA DAVID NOLTE, MD (PGY-2) HUSSAM AL-KATEB, PHD, FACMG DEBORAH FUCHS, MD OUTLINE High grade B-cell lymphoma with MYC and BCL2 and/or BCL6 rearrangements Patient presentation 2008/2016
More informationTest Utilization: Chronic Lymphocytic Leukemia
Test Utilization: Chronic Lymphocytic Leukemia Initial Evaluation Diagnostic Criteria Selection of Tests for Prognosis Response to Therapy Challenges Assessment for persistent disease Paul J. Kurtin, M.D.
More informationCritical Analysis and Diagnostic Usefulness of Limited Immunophenotyping of B-Cell Non-Hodgkin Lymphomas by Flow Cytometry
Hematopathology / FLOW CYTOMETRIC IMMUNOPHENOTYPING IN B-CELL NON-HODGKIN LYMPHOMA Critical Analysis and Diagnostic Usefulness of Limited Immunophenotyping of B-Cell Non-Hodgkin Lymphomas by Flow Cytometry
More informationFollicular Lymphoma: the WHO
Follicular Lymphoma: the WHO and the WHERE? Yuri Fedoriw, MD Associate Professor of Pathology and Laboratory Medicine Director of Hematopathology University of North Carolina Chapel Hill, NC Disclosure
More informationLYMPHOMAS an overview of some subtypes of NHLs
One of the confusing aspects of the lymphoid neoplasms concerns the use of the descriptive terms "leukemia" and "lymphoma." LYMPHOMAS an overview of some subtypes of NHLs Leukemia is used for lymphoid
More informationISIMM Tata Conference on Immunohistochemistry. Kolkata, India, January Immunohistochemistry. A cost effective approach to lymphoma diagnosis
ISIMM Tata Conference on Immunohistochemistry. Kolkata, India, January 2018 Immunohistochemistry A cost effective approach to lymphoma diagnosis Clive R. Taylor, M.D., Ph.D., Department of Pathology, Keck
More informationHODGKIN LYMPHOMA DR. ALEJANDRA ZARATE OSORNO HOSPITAL ESPAÑOL DE MEXICO
HODGKIN LYMPHOMA DR. ALEJANDRA ZARATE OSORNO HOSPITAL ESPAÑOL DE MEXICO HODGKIN LYMPHOMA CLASSIFICATION Lukes & Butler Rye WHO-2016 Linphocytic and/or histiocytic Nodular & diffuse Nodular Sclerosis Lymphocyte
More information11/2/2017. Immunodeficiencies. Joo Y. Song, MD Assistant Professor of Clinical Pathology. I have no financial disclosures.
I have no financial disclosures Joo Y. Song, MD Assistant Professor of Clinical Pathology City of Hope National Medical Center Immunodeficiencies Transplant Autoimmunity Drugs T-cell dysfunction (Age,
More informationDoes the proliferation fraction help identify mature B cell lymphomas with double- and triple-hit translocations?
Histopathology 2012, 61, 1214 1218. DOI: 10.1111/j.1365-2559.2012.04351.x SHORT REPORT Does the proliferation fraction help identify mature B cell lymphomas with double- and triple-hit translocations?
More informationSociety for Hematopathology 2017
Society for Hematopathology 2017 Session 2 Genetic Testing in the Diagnosis of Lymphoid Neoplasms Summary of the cases Session Chairs: Rebecca King and Miguel Piris September 7, 2017 Lymphoma diseases/variants
More information10/31/2017. Immunodeficiencies. Outline. Discuss EBV. Non-destructive Polymorphic Monomorphic Therapies Challenges
I have no financial disclosures Joo Y. Song, MD Assistant Professor of Clinical Pathology City of Hope National Medical Center Immunodeficiencies Outline Transplant Congenital Autoimmunity T-cell/immune
More informationB cell activation and antibody production. Abul K. Abbas UCSF
1 B cell activation and antibody production Abul K. Abbas UCSF 2 Lecture outline B cell activation; the role of helper T cells in antibody production Therapeutic targeting of B cells 3 Principles of humoral
More informationSession 5. Pre-malignant clonal hematopoietic proliferations. Chairs: Frank Kuo and Valentina Nardi
Session 5 Pre-malignant clonal hematopoietic proliferations Chairs: Frank Kuo and Valentina Nardi Pre-malignant clonal hematopoietic proliferations Clonal LYMPHOID proliferations: - Monoclonal gammopathy
More informationCase Report A case of EBV positive diffuse large B-cell lymphoma of the adolescent
Int J Clin Exp Med 2014;7(1):307-311 www.ijcem.com /ISSN:1940-5901/IJCEM1311029 Case Report A case of EBV positive diffuse large B-cell lymphoma of the adolescent Qilin Ao 2, Ying Wang 1, Sanpeng Xu 2,
More informationDaratumumab, a novel human CD38 monoclonal antibody for the treatment of B cell Non Hodgkin Lymphoma
Daratumumab, a novel human CD38 monoclonal antibody for the treatment of B cell Non Hodgkin Lymphoma Anna Vidal Department of Hemato Oncology IDIBAPS, Barcelona, Spain B cell lymphoid malignancies seen
More informationAggressive B-cell Lymphomas
Neoplastic Hematopathology Update 2018 Aggressive B-cell Lymphomas Raju K. Pillai City of Hope National Medical Center I do not have any disclosures Disclosures Outline New entities and changes in WHO
More informationDiagnostic Molecular Pathology of Lymphoid Neoplasms
Diagnostic Molecular Pathology of Lymphoid Neoplasms (Part II) Rational use of molecular testing in lymphomas Beirut, Lebanon Friday December 2, 2011: Hematopathology Session Adam Bagg University of Pennsylvania
More informationBurkitt lymphoma. Sporadic Endemic in Africa associated with EBV Translocations involving MYC gene on chromosome 8
Heme 8 Burkitt lymphoma Sporadic Endemic in Africa associated with EBV Translocations involving MYC gene on chromosome 8 Most common is t(8;14) Believed to be the fastest growing tumor in humans!!!! Morphology
More informationWHO 4th ED Classification of Mature B-cell Neoplasms
WHO 4th ED Classification of Mature B-cell Neoplasms Chronic lymphocytic leukemia /Small lymphocytic lymphoma B-cell prolymphocytic leukaemia Splenic marginal zone lymphoma Hairy cell leukemia Splenic
More informationAcquired Immunity 2. - Vaccines & Immunological Memory - Wataru Ise. WPI Immunology Frontier Research Center (IFReC) Osaka University.
Acquired Immunity 2 - Vaccines & Immunological Memory - Wataru Ise WPI Immunology Frontier Research Center (IFReC) Osaka University Outline 1. What is vaccine (vaccination)? 2. What is immunological memory?
More informationDefensive mechanisms include :
Acquired Immunity Defensive mechanisms include : 1) Innate immunity (Natural or Non specific) 2) Acquired immunity (Adaptive or Specific) Cell-mediated immunity Humoral immunity Two mechanisms 1) Humoral
More informationPatterns of Lymphoid Neoplasia in Peripheral Blood. Leon F. Baltrucki, M.D. Leon F. Baltrucki, M.D. Disclosure
Patterns of Lymphoid Neoplasia in Peripheral Blood Leon F. Baltrucki, M.D. Leon F. Baltrucki, M.D. Disclosure Dr Baltrucki has received an honorarium for his participation as a faculty presenter in this
More informationThe patient had a mild splenomegaly but no obvious lymph node enlargement. The consensus phenotype obtained from part one of the exercise was:
Case History An 86 year old male was admitted to hospital with chest infection. Haematological examination subsequently revealed the following: Hb- 11.0 g/dl; WBC- 67.1 x 10^9/l; PLT- 99 x10^9/l; RBC-
More informationDefined lymphoma entities in the current WHO classification
Defined lymphoma entities in the current WHO classification Luca Mazzucchelli Istituto cantonale di patologia, Locarno Bellinzona, January 29-31, 2016 Evolution of lymphoma classification Rappaport Lukes
More informationAdaptive Immunity: Humoral Immune Responses
MICR2209 Adaptive Immunity: Humoral Immune Responses Dr Allison Imrie 1 Synopsis: In this lecture we will review the different mechanisms which constitute the humoral immune response, and examine the antibody
More informationDevelopment of B and T lymphocytes
Development of B and T lymphocytes What will we discuss today? B-cell development T-cell development B- cell development overview Stem cell In periphery Pro-B cell Pre-B cell Immature B cell Mature B cell
More informationNEW ENTITIES IN AGGRESSIVE B CELL LYMPHOMA. Joon Seong Park, M.D. Dept. of Hematology-Oncology Ajou University School of Medicine
NEW ENTITIES IN AGGRESSIVE B CELL LYMPHOMA Joon Seong Park, M.D. Dept. of Hematology-Oncology Ajou University School of Medicine Historical background of Lymphoma classification Rappaport classification
More informationHigh grade B-cell lymphomas (HGBL): Altered terminology in the 2016 WHO Classification (Update of the 4 th Edition) and practical issues Xiao-Qiu Li,
High grade B-cell lymphomas (HGBL): Altered terminology in the 2016 WHO Classification (Update of the 4 th Edition) and practical issues Xiao-Qiu Li, M.D., Ph.D. Fudan University Shanghai Cancer Center
More informationAnaplastic Large Cell Lymphoma (of T cell lineage)
Anaplastic Large Cell Lymphoma (of T cell lineage) Definition T-cell lymphoma comprised of large cells with abundant cytoplasm and pleomorphic, often horseshoe-shaped nuclei CD30+ Most express cytotoxic
More informationMimics of Lymphoma in Routine Biopsies. Mixed follicular and paracortical hyperplasia. Types of Lymphoid Hyperplasia
Mimics of Lymphoma in Routine Biopsies Patrick Treseler, MD, PhD Professor of Pathology University of California San Francisco Types of Lymphoid Hyperplasia Follicular hyperplasia (B-cells) Paracortical
More information9/28/2017. Follicular Lymphoma and Nodal Marginal Zone Lymphoma. Follicular Lymphoma Definition. Low-Grade B-Cell Lymphomas in WHO Classification
and L. Jeffrey Medeiros, MD DISCLOSURES I do not have anything to disclose Low-Grade B-Cell Lymphomas in WHO Classification Lymphoma Type Frequency Follicular lymphoma 22.1 % Extranodal MALT-lymphoma 7.6
More informationCase year old male with abdominal lymphadenopathy Treated with 8 cycles of R-CHOP One year later B-symptoms and progressive disease
Codirectors Tsieh Sun, M.D., FASCP Francisco Vega, M.D., Ph.D. Department of Hematopathology UT MD Anderson Cancer Center Houston Texas There is no conflict of interest involved in the content and presentation
More informationLarge cell immunoblastic Diffuse histiocytic (DHL) Lymphoblastic lymphoma Diffuse lymphoblastic Small non cleaved cell Burkitt s Non- Burkitt s
Non Hodgkin s Lymphoma Introduction 6th most common cause of cancer death in United States. Increasing in incidence and mortality. Since 1970, the incidence of has almost doubled. Overview The types of
More informationVENTANA hematopathology solutions. Deliver diagnostic confidence
VENTANA hematopathology solutions Deliver diagnostic confidence 2 Hematopathology diagnostic solutions Contents VENTANA hematopathology assays 3 Detecting and subtyping hematological cancers 4 The importance
More informationMimics of Lymphoma in Routine Biopsies. I have nothing to disclose regarding the information to be reported in this talk.
Mimics of Lymphoma in Routine Biopsies Patrick Treseler, MD, PhD Professor of Pathology University of California San Francisco I have nothing to disclose regarding the information to be reported in this
More informationChange Summary - Form 2018 (R3) 1 of 12
Summary - Form 2018 (R3) 1 of 12 Form Question Number (r3) Type Description New Text Previous Text Today's date was removed 2018 N/A Today's Date Removed from Key Fields 2018 N/A HCT Type 2018 N/A Product
More informationAllergy and Immunology Review Corner: Chapter 1 of Immunology IV: Clinical Applications in Health and Disease, by Joseph A. Bellanti.
Allergy and Immunology Review Corner: Chapter 1 of Immunology IV: Clinical Applications in Health and Disease, by Joseph A. Bellanti. Chapter 1: Overview of Immunology Prepared by David Scott, MD, Scripps
More informationVENTANA hematopathology solutions Comprehensive aids for detecting and subtyping
VENTANA hematopathology solutions Comprehensive aids for detecting and subtyping 1 12/4/2015 9:47:24 AM 2 Hematopathology diagnostic solutions Contents VENTANA hematopathology assays 3 Detecting and subtyping
More informationGray Zones and Double Hits Distinguishing True Burkitt Lymphoma from Other High-Grade B-NHLs Burkitt Lymphoma Burkitt-Like Lymphoma DLBCL Patrick Tres
Gray Zones and Double Hits Distinguishing True Burkitt Lymphoma from Other High-Grade B-NHLs Burkitt Lymphoma Burkitt-Like Lymphoma DLBCL Patrick Treseler, MD, PhD University of California San Francisco
More informationLymph node cytopathology : A practical approach to lymphoproliferative disorders
Lymph node cytopathology : A practical approach to lymphoproliferative disorders Koray Ceyhan, M.D Department of Pathology Faculty of Medicine Ankara University Ankara, Turkey Diagnostic use of FNA in
More informationMolecular Diagnosis. Nucleic acid based testing in Oncology
Molecular Diagnosis Nucleic acid based testing in Oncology Objectives Describe uses of NAT in Oncology Diagnosis, Prediction, monitoring. Genetics Screening, presymptomatic testing, diagnostic testing,
More informationRecent advances in the genetics & biology of lymphoma
Recent advances in the genetics & biology of lymphoma Chris Bacon Northern Institute for Cancer Research Newcastle University & Newcastle Upon Tyne Hospitals NHS Foundation Trust Lymphoma Rate per 100,000
More informationNucleic Acid Testing - Oncology. Molecular Diagnosis. Gain/Loss of Nucleic Acid. Objectives. MYCN and Neuroblastoma. Molecular Diagnosis
Nucleic Acid Testing - Oncology Molecular Diagnosis Nucleic acid based testing in Oncology Gross alterations in DNA content of tumors (ploidy) Gain/Loss of nucleic acids Markers of Clonality Oncogene/Tumor
More informationPlasma cell myeloma (multiple myeloma)
Plasma cell myeloma (multiple myeloma) Common lymphoid neoplasm, present at old age (70 years average) Remember: plasma cells are terminally differentiated B-lymphocytes that produces antibodies. B-cells
More informationMonoclonal B-cell Lymphocytosis
Entity Centred Approach Lymphoma Classification: WHO and Beyond Clinically meaningful categories Dr Stefan Dojcinov University Hospital of Wales, Cardiff WHO UPDATE - NEW ENTITIES Early lesions lymphoma
More information