Fig. 39 FT-IR Analysis of column extract (F4) of B.zeylanica Fig. 40 FT-IR Analysis of column extract (F5) of B.zeylanica
Fig. 41 FT-IR Analysis of column extract (F1) of P.persica Fig. 42 FT-IR Analysis of column extract (F2) of P.persica
Fig. 43 FT-IR Analysis of column extract (F3) of P.persica Fig. 44 FT-IR Analysis of column extract (F5) of P.persica
Fig. 45 FT-IR Analysis of column extract (F1) of C.virgineus Fig. 46 FT-IR Analysis of column extract (F2) of C.virgineus
Fig. 47 1 HNMR Spectrum of column extract (F4) of B.zeylanica Expanded
Fig. 48 1 HNMR Spectrum of column extract (F5) of B.zeylanica Expanded
Fig. 49 1 HNMR Spectrum of column extract (F1) of P.persica Expanded
Fig. 50 1 HNMR Spectrum of column extract (F2) of P.persica Expanded
Fig. 51 1 HNMR Spectrum of column extract (F3) of P.persica Expanded
Fig. 52 1 HNMR Spectrum of column extract (F5) of P.persica Expanded
Fig. 53 1 HNMR Spectrum of column extract (F1) of C.virgineus Expanded
Fig. 54 1 HNMR Spectrum of column extract (F2) of C.virgineus Expanded
Fig. 55 Chromatogram of column extract (F4) of B.zeylanica by GC-MS Fig. 55 a Chromatogram Compounds in F4 fraction of B.zeylanica 100 69 á-d-mannofuranoside, farnesyl- HO C 21 H 36 O 6 50 41 81 93 HO O O OH OH 55 107121 27 135 161 175 189 204 221 0 20 50 80 110 140 170 200 230 260 290 320 350 380 (mainlib) á-d-mannofuranoside, farnesyl- Fig. 55 b 2-Piperidnone C 5 H 9 NO
Fig. 55 c 9-Octadecenamide, (z)- C18H35NO Fig. 55 d 1,2-Benzenedicarboxylic acid, diisooctyl ester C 24H 38O 4 Fig. 56 Chromatogram of column extract (F5) of B.zeylanica by GC-MS
Fig. 56 a Chromatogram Compounds in F5 fraction of B.zeylanica 100 50 43 55 69 73 81 91 95 109 121 133 149 177 193 238 0 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 (mainlib) 2,2-Dimethyl-6-methylene-1-[3,5-dihydroxy-1-pentenyl]cyclohexan-1-perhydrol Fig. 56 b 2,2-Dimethyl-6-methylene-1-[3,5-dihydroxy-1- pentenyl]cyclohexan-1-perhydrol 160 O OH 203 OH OH 221 C 14 H 24 O 4 Diethyl Phthalate C 12 H 14 O 4 Fig. 56 c 100 41 93 119 trans-à-bergamotene C15H24 50 39 55 69 77 91 107 53 29 43 67 51 81 133 161 89 147 189 0 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 (mainlib) trans-à-bergamotene
Fig. 57 Chromatogram of column extract (F1) of P.persica by GC-MS Chromatogram Compounds in F1 fraction of P.persica Fig. 57 a 7,8 Epoxylanostan-11-ol, 3-acetoxy C32H54O4 Fig. 57 b Ethanethiol, 2-phenoxy- C8H10OS
Fig. 58 Chromatogram of column extract (F2) of P.persica by GC-MS Fig. 58 a Chromatogram Compounds in F2 fraction of P.persica 9,12-Octadecadienoyl chloride, (Z,Z)- C18H31ClO Chromatogram of column extract (F3) of P.persica by GC-MS Fig. 59
Fig. 59 a Chromatogram Compounds in F3 fraction of P.persica Eugenol C10H12O2 Fig. 59 b 1,2-Benzenedicarboxylic acid, diisooctyl ester C24H38O4 Fig. 59 c Phthalic acid, bis (7-methyloctyl) ester C26H42O4
Chromatogram of column extract (F5) of P.persica by GC-MS Fig. 60 Chromatogram Fig. 60 a Compounds in F5 fraction of P.persica Azulene C10H8 Fig. 60 b 1-Octanol, 2,7-dimethyl- C10H22O
Fig. 60 c 2-Piperidinone C5H9NO Fig. 60 d Cholest-5-ene, 3-bromo-, (3.beta.)- C27H45Br Chromatogram of column extract (F1) of C.virgineus by GC-MS Fig. 61
Fig. 61 a Chromatogram Compounds in F1 fraction of C.virgineus Diethyl Phthalate C12H14O4 Fig. 61 b Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro- C7H10N2O2 Fig. 61 c 9-Dodecenoic acid, methyl ester, (E)- C13H24O2
Chromatogram of column extract (F2) of C.virgineus by GC-MS Fig. 62 Fig. 62 a Chromatogram Compounds in F2 fraction of C.virgineus Perfluoro-n-pentanoic acid C5HF9O2 Fig. 62 b Perfluorotributylamine C12F27N
Fig. 62 c Ethanol, 2-(hexadecyloxy)- C18H38O2
Fig. 63 HPLC chromatogram of isolated compound of B.zeylanica compared to the standard diethyl phthalate
Fig. 64 HPLC chromatogram of isolated compound of B.zeylanica compared to the standard oleic acid
Fig. 65 HPLC chromatogram of isolated compound of P.persica compared to the standard azulene
Fig. 66 HPLC chromatogram of isolated compound of P.persica compared to the standard eugenol
Fig. 67 HPLC chromatogram of isolated compound of P.persica compared to the standard oleic acid
Fig. 68 HPLC chromatogram of isolated compound of P.persica compared to the standard diethyl phthalate
Fig. 69 HPLC chromatogram of isolated compound of C.virgineus compared to the standard oleic acid
Fig. 70 HPLC chromatogram of isolated compound of C.virgineus compared to the standard diethyl phthalate
Table 1 Showing various activities of identified compounds by GC-MS in experimental animals B.zeylanica, P.persica and C.virgineus B.zeylanica (F 4 ) Benzene : Methanol S.No RT Name of the compound Peak Area % 1 2.32 Glycerin 20.48 Alcohol 2 4.30 2-Piperidinone 6.55 Alkaloid 3 9.19 Diethyl Phthalate 0.73 4 12.67 5 12.91 9-Hexadecenoic acid, methyl ester, (Z)- Hexadecanoic acid, methyl ester Compound Nature Plasticizer compound 1.83 Oleic acid ester 4.66 Palmitic acid ester * Activity 6 16.34 9-Octadecenamide, (Z)- 0.94 Amide compound 7 17.70 Undecanal, 2-methyl- 0.94 Aldehyde compound 8 18.04 Oleic Acid 0.73 Oleic acid 9 21.27 10 22.34 11 26.49 1,2-Benzenedicarboxylic acid, diisooctyl ester 3-Hexadecyloxycarbonyl- 5-(2-hydroxyethyl)-4- methylimidazolium ion á-d-mannofuranoside, farnesyl- 2.46 Plastici8zer compound 3.88 Nitrogen compound 18.12 Terpene compound 12 29.40 Cholesterol 16.82 Steroid Preservative Anti-inflammatory Anti fouling Anti-inflammatory, Antiandrogenic, Cancer preventive, Dermatitigenic, Hypocholesterolemic, 5-Alpha reductase inhibitor, Anemiagenic, Insectifuge, Flavor Antioxidant, Hypocholesterolemic Nematicide, Pesticide, Lubricant, Antiandrogenic, Flavor, Hemolytic 5-Alpha reductase inhibitor Anti-inflammatory, Antiandrogenic, Cancer preventive, Dermatitigenic, Hypocholesterolemic, 5-Alpha reductase inhibitor, Anemiagenic, Insectifuge, Flavor Anti fouling Anti-inflammatory Anticancer Antiasthma Anti-inflammatory
Table 2 B.zeylanica (F 5 ) Methanal RT Name of the compound Peak Area % Compound Nature 1 8.22 trans-à-bergamotene 2.35 Sesquiterpene 2 9.19 Diethyl Phthalate 4.70 Plasticizer compound 3 11.02 4 21.68 5 30.38 Phenol, 2-methyl-5- (1,2,2- trimethylcyclopentyl)-, (S)- 1,2- Benzenedicarboxylic acid, diisooctyl ester 2,2-Dimethyl-6- methylene-1-[3,5- dihydroxy-1- pentenyl]cyclohexan- 1-perhydrol 2.98 Phenolic compound 78.27 Plasticizer compound *Activity Anti-inflammatory Antifouling Anti-inflammatory Antioxidant Antifouling 5.78 Alcoholic compound Table 3 P.persica (F 1 ) Hexane : Chloroform S.No RT Name of the compound Peak Area % 1 16.00 7,8-Epoxylanostan-11-ol, 3-acetoxy- 4.40 2 19.36 2-Nonadecanone 2,4-dinitrophenylhydrazine 8.79 Compound Nature Alcoholic compound Nitrogen compound *Activity 3 2.34 Ethanethiol, 2-phenoxy- 16.78 Sulfur compound
Table 4 P.persica (F 2 ) Chloroform S.No RT Name of the compound Peak Area % Nature of compound *Activity 1 17.90 9,12-Octadecadienoyl chloride, (Z,Z)- 2.34 Chloride compound 2 20.17 3 29.40 9,12,15-Octadecatrienoic acid, methyl ester, (Z,Z,Z)- Cholest-5-en-3-ol (3á)-, carbonochloridate 1.52 Linoleic acid ester 72.31 Steroid Anti-inflammatory, Hypocholesterolemic, Cancer preventive Hepatoprotective Nematicide, Insectifuge Antihistaminic, Antieczemic Antiacne, 5-Alpha reductase inhibitor Antiandrogenic, Antiarthritic, Anticoronary, Insectifuge Antiasthma Anti-inflammatory Table 5 P.persica No RT Name of the compound Peak Area % 1 6.38 Eugenol 1.35 2 13.44 Dibutyl phthalate 1.40 3 21.51 4 25.36 1,2- Benzenedicarboxylic acid, diisooctyl ester Phthalic acid, bis(7- methyloctyl) ester 79.95 9.70 Compound Nature Phenolic compound Plasticizer compound Plasticizer compound Plasticizer compound (F 3 ) Benzene *Activity Analgesic, Anesthetic, Allergenic, Antibacterial, Anticonvulsant, Anti-inflammatory, Antioxidant, Antipyretic, Antisalmonella, Antistaphylococc, Antiseptic Antifouling Antifouling Antifouling
Table 6 P.persica (F 5 ) Methanol S.No RT Name of the compound Peak Area % Nature of compound 1 2.33 Glycerin 38.68 Alcoholic compound 2 4.32 2-Piperidinone 12.89 Alkaloid compound 3 5.54 Acetic acid, trifluoro-, tetradecyl ester *Activity Preservative Anti-inflammatory 4.88 Fluro compound 4 5.76 1-Octanol, 2,7-dimethyl- 5.57 Alcoholic compound 5 4.40 Azulene 0.55 Monoterpene 6 29.62 Cholest-5-ene, 3- bromo-, (3á)- 18.17 Steroid Anti-tumor, Analgesic, Antibacterial Antiinflammatory, Sedative, Fungicide, Hypocholesterolemic, Insecticide, Insectifuge Chemopreventive, Pesticide, Antiacne, Nematicide Anti-inflammatory Anticancer Diuretic Antiasthma
Table 7 C.virgineus (F 1 ) Hexane: Chloroform No RT Name of the compound 1 2.34 Dimethylaminomethylisopropyl-sulfide Peak Area % 4.75 Compound Nature Amino compound with sulfur 2 4.57 2-Piperidinone 0.39 Alkaloid 3 9.28 Diethyl Phthalate 3.17 4 11.38 Pyrrolo[1,2-a]pyrazine-1,4- dione, hexahydro- 5 13.12 Hexadecanoic acid, methyl ester 6 15.48 9-Dodecenoic acid, methyl ester, (E)- 7 17.79 5,8,11,14- Eicosatetraenoic acid, ethyl ester, (all-z)- 8 20.92 8,11,14-Eicosatrienoic acid, (Z,Z,Z)- 9 31.99 9,10-Secochola- 5,7,10(19)-trien-24-al, 3- hydroxy-, (3á,5Z,7E)- Plasticizer compound 0.59 Alkaloid 2.58 Palmitic acid ester 0.88 Oleic acid ester 4.68 1.62 Unsaturated fatty acid ester Unsaturated fatty acid ester 7.24 Steroid * Activity Anti-inflammatory Anti fouling Anti-inflammatory Antioxidant, Pesticide, Hypocholesterolemic, Nematicide, Lubricant, Antiandrogenic, Flavor, Hemolytic. Anti-inflammatory, Antiandrogenic Cancer preventive, Dermatitigenic Hypocholesterolemic, 5-Alpha reductase inhibitor, Anemiagenic Insectifuge, Flavor Anticholesterol Anticholesterol Anti-inflammatory Antiasthma Diuretic
Table 8 C.virgineus (F 2 ) Chloroform No RT Name of the compound Peak Area % Compound Nature * Activity 1 9.25 Diethyl Phthalate 14.74 Plasticizer compound Antifouling 2 12.99 Perfluorotributylamine 4.50 Amino compound 3 17.44 4 19.51 Perfluoro-n-pentanoic acid 7.19 Fluro compound Ethanol, 2- (hexadecyloxy)- 11.07 Alcoholic compound * Source: Dr.Duke's Phytochemical and Ethnobotanical Databases