Supplementary Table 1. Genes analysed for expression by angiogenesis gene-array. Gene symbol Gene name TaqMan Assay ID UniGene ID 18S rrna 18S ribosomal RNA Hs99999901_s1 Actb actin, beta Mm00607939_s1 Mm.328431, Mm.391967, Mm.469717 Actb actin, beta Mm02619580_g1 Mm.328431, Mm.391967, Mm.469717 Angpt1 angiopoietin 1 Mm01129232_m1 Mm.309336 Angpt2 angiopoietin 2 Mm00545822_m1 Mm.439874 Anpep alanyl (membrane) Mm00476227_m1 Mm.4487 aminopeptidase Bai1 brain-specific Mm00558144_m1 Mm.43133 angiogenesis inhibitor 1 Ccl11 chemokine (C-C motif) Mm00441238_m1 Mm.4686 ligand 11 Ccl2 chemokine (C-C motif) Mm99999056_m1 Mm.290320 ligand 2 Cdh5 cadherin 5 Mm03053719_s1 Mm.21767 Col18a1 collagen, type XVIII, Mm00487131_m1 Mm.4352 alpha 1 Col4a3 collagen, type IV, alpha Mm00483656_m1 Mm.389135 3 Csf3 colony stimulating factor Mm00438335_g1 Mm.1238 3 (granulocyte) Ctgf connective tissue growth Mm00515790_g1 Mm.393058 factor Cxcl1 chemokine (C-X-C Mm00433859_m1 Mm.21013 motif) ligand 1 Cxcl2 chemokine (C-X-C Mm00436450_m1 Mm.4979 motif) ligand 2 Cxcl5 chemokine (C-X-C Mm00436451_g1 Mm.4660 motif) ligand 5 Efna1 ephrin A1 Mm00438660_m1 Mm.15675 Efnb2 ephrin B2 Mm00438670_m1 Mm.209813 Egf epidermal growth factor Mm01316968_m1 Mm.252481 Eng endoglin Mm03023983_m1 Mm.225297 Epas1 endothelial PAS domain Mm00438717_m1 Mm.1415 protein 1 Ephb4 Eph receptor B4 Mm00438750_m1 Mm.34533 Ereg epiregulin Mm00514794_m1 Mm.4791 F2 coagulation factor II Mm00438843_m1 Mm.89048 Fgf1 fibroblast growth factor 1 Mm03053735_s1 Mm.241282 Fgf2 fibroblast growth factor 2 Mm00433287_m1 Mm.473689 Fgf6 fibroblast growth factor 6 Mm01183111_m1 Mm.3403 Fgfr3 fibroblast growth factor Mm03003505_s1 Mm.6904 receptor 3 Figf c-fos induced growth factor Mm00438963_m1 Mm.297978
Flt1 FMS-like tyrosine kinase Mm00438971_m1 Mm.389712 1 Fzd5 frizzled homolog 5 (Drosophila) Mm03053323_s1 Mm.150813, Mm.470210 Gapdh glyceraldehyde-3- Mm03302249_g1 Mm.304088 phosphate dehydrogenase Gna13 guanine nucleotide Mm00494667_m1 Mm.193925 binding protein, alpha 13 Gusb glucuronidase, beta Mm00446953_m1 Mm.3317 Gusb glucuronidase, beta Mm03003537_s1 Mm.3317 Hand2 heart and neural crest Mm00439247_m1 Mm.430844 derivatives expressed transcript 2 Hgf hepatocyte growth factor Mm00690363_m1 Mm.267078 Hif1a hypoxia inducible factor 1, alpha subunit Mm00468878_m1 Mm.3879, Mm.446610 Hprt1 hypoxanthine guanine Mm00446968_m1 Mm.299381 phosphoribosyl transferase 1 Hsp90ab1 heat shock protein 90 Mm00833431_g1 Mm.2180 alpha (cytosolic), class B member 1 Iapp islet amyloid polypeptide Mm00439403_m1 Mm.415 Ifng interferon gamma Mm99999071_m1 Mm.240327 Igf1 insulin-like growth factor Mm01233960_m1 Mm.268521 1 Il1b interleukin 1 beta Mm01336189_m1 Mm.222830 Il6 interleukin 6 Mm99999064_m1 Mm.1019 Itgav integrin alpha V Mm00434506_m1 Mm.227 Itgb3 integrin beta 3 Mm00443980_m1 Mm.87150 Jag1 jagged 1 Mm03053874_s1 Mm.22398 Kdr kinase insert domain Mm00440099_m1 Mm.285 protein receptor Lama5 laminin, alpha 5 Mm01222029_m1 Mm.4339 Lect1 leukocyte cell derived Mm00495291_m1 Mm.46561 chemotaxin 1 Lep leptin Mm00434759_m1 Mm.277072 Mapk14 mitogen-activated protein Mm00442497_m1 Mm.311337 kinase 14 Mdk midkine Mm00440279_m1 Mm.906 Mmp19 matrix metallopeptidase Mm00491300_m1 Mm.131266 19 Mmp2 matrix metallopeptidase Mm00439506_m1 Mm.29564 2 Mmp9 matrix metallopeptidase Mm00442991_m1 Mm.4406 9 Npr1 natriuretic peptide Mm00435324_m1 Mm.4627 receptor 1 Nrp1 neuropilin 1 Mm00435372_m1 Mm.271745, Mm.423531 Nrp2 neuropilin 2 Mm00803101_m1 Mm.266341 Pdgfa platelet derived growth Mm00833533_m1 Mm.2675
factor, alpha Pdx1 pancreatic and duodenal Mm00435565_m1 Mm.389714 homeobox 1 Pecam1 platelet/endothelial cell Mm00476702_m1 Mm.343951 adhesion molecule 1 Pgf placental growth factor Mm00435613_m1 Mm.4809 Plau plasminogen activator, Mm00447054_m1 Mm.4183 urokinase Plg plasminogen Mm00447087_m1 Mm.971 Plg plasminogen Mm01312967_m1 Mm.971 Ptgs1 prostaglandinendoperoxide Mm00477214_m1 Mm.275434 synthase 1 S1pr1 sphingosine-1-phosphate Mm00514644_m1 Mm.982 receptor 1 Serpinf1 serine (or cysteine) Mm00441270_m1 Mm.2044 peptidase inhibitor, clade F, member 1 Smad5 MAD homolog 5 Mm01341687_g1 Mm.272920 (Drosophila) Sphk1 sphingosine kinase 1 Mm00448841_g1 Mm.20944 Stab1 stabilin 1 Mm00460390_m1 Mm.220821 Tbp TATA box binding Mm00446973_m1 Mm.244820 protein Tbp TATA box binding Mm01277045_m1 Mm.244820 protein Tbx1 T-box 1 Mm00448948_m1 Mm.295194 Tbx4 T-box 4 Mm00550370_m1 Mm.275336 Tek endothelial-specific Mm00443242_m1 Mm.14313 receptor tyrosine kinase Tgfa transforming growth Mm00446231_m1 Mm.137222 factor alpha Tgfb1 transforming growth Mm03024053_m1 Mm.248380 factor, beta 1 Tgfb2 transforming growth Mm03024009_m1 Mm.18213 factor, beta 2 Tgfb3 transforming growth Mm01307950_m1 Mm.3992 factor, beta 3 Tgfbr1 transforming growth factor, beta receptor I Mm03024015_m1 Mm.197552, Mm.452145 Thbs1 thrombospondin 1 Mm01335418_m1 Mm.4159 Thbs2 thrombospondin 2 Mm00449036_m1 Mm.26688 Timp1 tissue inhibitor of Mm01341361_m1 Mm.8245 metalloproteinase 1 Timp2 tissue inhibitor of Mm00441825_m1 Mm.206505 metalloproteinase 2 Tmprss6 transmembrane serine Mm00551108_m1 Mm.34056 protease 6 Tnf tumor necrosis factor Mm00443258_m1 Mm.1293 Tnfaip2 tumor necrosis factor, Mm00447578_m1 Mm.255332 alpha-induced protein 2 Tnfsf12 tumor necrosis factor (ligand) superfamily, Mm00839900_m1 Mm.8983
member 12 Tymp thymidine phosphorylase Mm00460357_m1 Mm.287977 Vegfa vascular endothelial growth factor A Mm00437304_m1 Mm.282184, Mm.477156 Vegfb vascular endothelial Mm00442100_g1 Mm.15607 growth factor B Vegfc vascular endothelial growth factor C Mm00437310_m1 Mm.1402
Supplementary Table 2. Characteristics of islets for transplantation. Islet purity as assessed by dithizone (%) 1 day 4 days Intact Fragmented Intact 95±1 88±3* 100±0 Insulin release ng/10 islets x h Low glucose (1.67 mmol/l) High glucose (16.7 mmol/l) 15±3 34±6* 12±4 68±4 94±7* 72±6 All values are given as means ± SEM for 6-7 experiments in each group. * denotes P< 0.05 when compared to 1-day intact islets. Supplementary Figure 1. Representative images of Oil Red O staining of cryosections obtained from liver of high fat diet treated mice (positive control; A), and islet graft bearing liver (B), respectively. Arrow in B depicts islet. Scale bar is 100 µm.