CURRICULUM VITAE PERSONAL DETAILS:

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1 CURRICULUM VITAE PERSONAL DETAILS: NAME: Theerapong Krajaejun SEX: Male NATIONALLITY: Thai MARITAL STATUS: Single POSITION: Assistant Professor, ADDRESS: Department of Pathology, Faculty of Medicine-Ramathibodi Hospital, Mahidol University, Bangkok, 10400, Thailand. Address: EDUCATIONS: 1999 Doctor of Medicine Faculty of Medicine-Ramathibodi Hospital, Mahidol University, Bangkok, Thailand Thai Board of Clinical Pathology Department of Pathology, Faculty of Medicine-Ramathibodi Hospital, Mahidol University, Bangkok, Thailand Postdoctoral fellow University of Wisconsin-Madison, USA APPOINTMENT AND AFFLILIATION: 1999-present Instructor Department of Pathology Faculty of Medicine Ramathibodi Hospital Mahidol University, Thailand HONORS AND AWARDS: (1) The A.G. Ellis Award for the best research presentation, the 13th National Congress of Pathology, Rachavitee Hospital, Bangkok, Thailand (Mar 2001). (2) The best research presentation award, the 2001 Annual Meeting of Lerdsin Hospital, Bangkok, Thailand (Jun 2001). (3) The best pathology research presentation award, the 2001 Annual Meetings of Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand (May 2001).

2 - 2 - (4) The American Society for Microbiology-National Academy of Science (USA) Travel Award for a poster presentation at the 10th International Congress of Mycology, and for visiting the Institut Pasteur (Host: Dr. Anawaj Sakuntabhai), Paris, France (July-Aug 2002). (5) The 2002 UNESCO-American Society for Microbiology Travel Award for a research training on Pythium insidiosum at Michigan State University (Host: Dr. Leonel Mendoza), Michigan, USA (Sep-Oct 2002). (6) A contributor of the 2002 Imaging for Infectious Diseases at the 40th Annual Meeting of the Infectious Disease Society of America, Chicago, USA (Oct 2002). (7) The outstanding research presentation award, the 2003 Annual Meeting of the Infectious Disease Society of Thailand, Thailand (Oct 2003). (8) The 9th WPCCID young investigator award, the 9th Western Pacific Congress on Chemotherapy and Infectious Diseases, Bangkok, Thailand (Dec 2004). (9) The Ellison Medical Foundation-International Nutrition Foundation Fellowship toward a postdoctoral training at University of Wisconsin-Madison (Supervisor: Dr. Bruce S. Klein), Wisconsin, USA (2003-6). (10) The 2006/2007 Oomycete Molecular Genetics Fellowship Award (Sponsor: the Oomycete Molecular Genetics Research Collaboration Network and the National Science Foundation- USA) for a research training (transformation techniques for Pythium species) at Wageningen University (Host: Dr. Francine Govers), The Netherlands (will be in Apr-May 2007). RESEARCH FIELDS OF INTEREST: Clinical, cellular, and molecular mycology Microbial pathogenesis and host responses Host-pathogen interaction RESEARCH FUNDS: 2001, 3 Ramathibodi Foundation Young Faculty Supporting Grants (PI) Ramathibodi Research Grants (ID /02) (PI) Thai Research Fund (TRG ) (PI) Siriraj Research Grant for Faculty Development (Co-PI)

3 - 3 - RESEARCH WORKS: (1) Krajaejun T, Kunakorn M, Niemhom S, Chongtrakool P and Pracharktam R. Development and Evaluation of an In-House Enzyme-Linked Immunosorbent Assay for Early Diagnosis and Monitoring of Human Pythiosis. Clin Diagn Lab Immunol 2002; 9: (The 2002 Impact Factor, 1.654) (2) Krajaejun T, Pracharktam R, Wongwaisayawan S, Rochanawutinon M, Kunakorn M, and Kunavisarut S. Ocular pythiosis: is it under-diagnosed? Am J Ophthalmol 2004; 137: (The 2004 Impact Factor, 2.332) (3) Krajaejun T, Kunakorn M, Pracharktam R, Chongtrakool P, Sathapatayavongs B, Chaiprasert A, Vanittanakom N, Chindamporn A, and Mootsikapun P. Identification of a Novel 74-kDa Immunodominant Antigen of Pythium insidiosum Recognized by Sera from Human Patients with Pythiosis. J Clin Microbiol 2006; 44: (The 2005 Impact Factor, 3.537) (4) Krajaejun T, Sathapatayavongs B, Pracharktam R, Nitiyanant P, Leelachaikul P, Wanachiwanawin W, Chaiprasert A, Assanasen P, Saipetch M, Mootsikapun P, Chetchotisakd P, Lekhakula A, Mitarnun W, Kalnauwakul S, Supparatpinyo K, Chaiwarith R, Chiewchanvit S, Tananuvat N, Srisiri S, Suankratay C, Kulwichit W, Wongsaisuwan M, and Somkaew S. Clinical and Epidemiological Analyses of Human Pythiosis in Thailand. Clin Infect Dis 2006; 43: (The 2005 Impact Factor, 6.510) (5) Pannanusorn S, Chaiprasert A, Prariyachatigul C, Krajaejun T, Vanittanakom N, Chindamporn A, Sathapatayavongs B, and Wanachiwanawin W. RAPD Typing and Phylogeny of Pythium insidiosum Clinical Isolates in Thailand (Submitted for publication). (6) Krajaejun T, Gauthier GM, Rappleye CA, Sullivan TD, and Klein BS. Development and Application of a Green Fluorescent Protein Sentinel System for Identification of RNA Interference in Blastomyces dermatitidis Illuminates the Role of Septin in Morphogenesis and Sporulation. Eukaryo Cell 2007; 6: (7) Krajaejun T, Sathapatayavongs B, Chaiprasert A, Srimuang S. Do you Know Human Pythiosis? J Infect Dis Antimicrob Agents 2008; 25: (8) Krajaejun T, Imkhieo S, Intaramat A and Ratanabanangkoon K. Development of an Immunochromatographic Test for Rapid Serodiagnosis of Human Pythiosis. (Submitted)

4 - 4 - (9) Chaiprasert A*, Krajaejun T*, Pannanusorn S, Prariyachatigul C, Wanachiwanawin W, Chindamporn A, Vanittanakom N, and Sathapatayavongs B. Genetic diversity of Pythium insidiosum Thai Isolates using rdna internal transcribed spacers sequences (Submitted) * These authors contributed equally to this work (10) Jindayok T, Piromsontikorn S, Srimuang S, Khupulsup K and Krajaejun T. An In- House Hemagglutination Test for Rapid Serodiagnosis and Treatment Monitoring of Human Pythiosis. (Manuscript in preparation) (11) Krajaejun T, Chongtrakool P and Angkananukul K. Effect of Temperature on Growth of the Human Pathogen Pythium insidiosum. (Manuscript in preparation) SCIENTIFIC PRESENTATIONS AND CONGRESS ATTENDANCES: (1) The 11th International Congress of Immunology, Stockholm, Sweden (Jul 2001). (2) The 10th International Congress of Mycology, Paris, France (Jul 2002). (3) The 40th Annual Meeting of the Infectious Disease Society of America, Chicago, USA (Oct 2002). (4) The 6th South Pacific Congress, Gold Coast, Australia (Oct 2003). (5) The symposium Fungal Pathogenesis: Bridging the Gap between the Bedside and the Bench, University of Wisconsin-Madison, USA (Sep 2004). (6) The 9th Western Pacific Congress on Chemotherapy and Infectious diseases, Bangkok, Thailand (Dec 2004). (7) The 2005 and 2006 Immunology Research Symposium, University of Wisconsin-Madison, USA (Jun 2005 and Jun 2006). (8) The First American Society for Microbiology Conference on Dimorphic Fungal Pathogens, Denver, USA (Mar 2006). (9) The 4th Chicago Area Mycology and Parasites Meeting, Chicago, USA (Apr 2006). (10) The First Annual Medical Microbiology and Immunology Research Retreat, University of Wisconsin-Madison, USA (Jun 2006). CURRENT RESEARCH PROJECTS: 1. Comparison of humoral immune response of vaccinated and unvaccinated pythiosis patients by immunoblotting technique

5 Identification and purification of immunodominant antigen of Pythium insidiosum for structural and functional studies, and its application in immunotherapy and diagnostic tests (immunoperoxidase staining, latex agglutination, ELISA) 3. Clinical Epidemiology of human pythiosis in Thailand: Multicentric Retrospective Study 4. Molecular epidemiology of Pythium insidiosum with clinical correlation PUBLICATIONS: (เร ยงจากป จจ บ น อด ต) 1. Keeratijarut A, Lohnoo T, Yingyong W, Sriwanichrak K, Krajaejun T.A peptide ELISA to detect antibodies against Pythium insidiosum based on predicted antigenic determinants of exo-1,3-beta-glucanase.southeast Asian J Trop Med Public Health Jul 4;44(4): Chareonsirisuthigul T, Khositnithikul R, Intaramat A, Inkomlue R, Sriwanichrak K, Piromsontikorn S, Kitiwanwanich S, Lowhnoo T, Yingyong W, Chaiprasert A, Banyong R, Ratanabanangkoon K, Brandhorst TT, Krajaejun T.Performance comparison of immunodiffusion, enzyme-linked immunosorbent assay, immunochromatography and hemagglutination for serodiagnosis of human pythiosis.diagn Microbiol Infect Dis May;76(1): Krajaejun T, Lowhnoo T, Yingyong W, Rujirawat T, Fucharoen S, Strobel GA.In vitro antimicrobial activity of volatile organic compounds from Muscodor crispans against the pathogenic oomycete Pythium insidiosum.southeast Asian J Trop Med Public Health Nov;43(6): Krajaejun T, Khositnithikul R, Lerksuthirat T, Lowhnoo T, Rujirawat T, Petchthong T, Yingyong W, Suriyaphol P, Smittipat N, Juthayothin T, Phuntumart V, Sullivan TD. Expressed sequence tags reveal genetic diversity and putative virulence factors of the pathogenic oomycete Pythium insidiosum. Fungal Biol Jul;115(7): Epub 2011 May Kammarnjesadakul P, Palaga T, Sritunyalucksana K, Mendoza L, Krajaejun T, Vanittanakom N, Tongchusak S, Denduangboripant J, Chindamporn A. Phylogenetic analysis of Pythium

6 - 6 - insidiosum Thai strains using cytochrome oxidase II (COX II) DNA coding sequences and internal transcribed spacer regions (ITS).Med Mycol Apr;49(3): Wüthrich M, Krajaejun T, Shearn-Bochsler V, Bass C, Filutowicz HI, Legendre AM, Klein BS. Safety, tolerability, and immunogenicity of a recombinant, genetically-engineered, liveattenuated vaccine against canine blastomycosis. Clin Vaccine Immunol May;18(5): Epub 2011 Mar Krajaejun T, Wüthrich M, Gauthier GM, Warner TF, Sullivan TD, Klein BS. Discordant influence of Blastomyces dermatitidis yeast-phase-specific gene BYS1 on morphogenesis and virulence. Infect Immun Jun;78(6): Epub 2010 Apr Krajaejun T, Keeratijarut A, Sriwanichrak K, Lowhnoo T, Rujirawat T, Petchthong T, Yingyong W, Kalambaheti T, Smittipat N, Juthayothin T, Sullivan TD. The 74-kilodalton immunodominant antigen of the pathogenic oomycete Pythium insidiosum is a putative exo-1,3-beta-glucanase. Clin Vaccine Immunol Aug;17(8): Epub 2010 Mar Kammarnjesadakul P, Palaga T, Sritunyalucksana K, Mendoza L, Krajaejun T, Vanittanakom N, Tongchusak S, Denduangboripant J, Chindamporn A. Phylogenetic analysis of Pythium insidiosum Thai strains using cytochrome oxidase II (COX II) DNA coding sequences and internal transcribed spacer regions (ITS). Med Mycol Sep 6. [Epub ahead of print] 10. Lévesque CA, Brouwer H, Cano L, Hamilton JP, Holt C, Huitema E, Raffaele S, Robideau GP, Thines M, Win J, Zerillo MM, Beakes GW, Boore JL, Busam D, Dumas B, Ferriera S, Fuerstenberg SI, Gachon CM, Gaulin E, Govers F, Grenville-Briggs L, Horner N, Hostetler J, Jiang RH, Johnson J, Krajaejun T, Lin H, Meijer HJ, Moore B, Morris P, Phuntmart V, Puiu D, Shetty J, Stajich JE, Tripathy S, Wawra S, van West P, Whitty BR, Coutinho PM, Henrissat B, Martin F, Thomas PD, Tyler BM, De Vries RP, Kamoun S, Yandell M, Tisserat N, Buell CR. Genome sequence of the necrotrophic plant pathogen Pythium ultimum reveals original

7 - 7 - pathogenicity mechanisms and effector repertoire.genome Biol. 2010;11(7):R73. Epub 2010 Jul Jindayok T, Piromsontikorn S, Srimuang S, Khupulsup K, Krajaejun T. Hemagglutination test for rapid serodiagnosis of human pythiosis. Clin Vaccine Immunol Jul;16(7): Krajaejun T, Imkhieo S, Intaramat A, Ratanabanangkoon K. Development of an immunochromatographic test for rapid serodiagnosis of human pythiosis. Clin Vaccine Immunol.2009 Apr;16(4): Krajaejun T, Sathapatayavongs B, Chaiprasert A, Srimuang S. Do you Know Human Pythiosis? J Infect Dis Antimicrob Agents 2008; 25: Srisuda Pannanusorn, Angkana Chaiprasert, Chularut Prariyachatigul, Theerapong Krajaejun, Nongnuch Vanittanakom, Ariya Chindamporn, Wanchai Wanachiwanawin, Boonmee Satapatayavong. Random amplified polymorphic DNA typing and phylogeny of Pythium insidiosum clinical isolates in Thailand. Southeast Asian J Trop Med Public Health 2007 Mar; 38(2): T Krajaejun, G M Gauthier, C A Rappleye, T D Sullivan, B S Klein. The development and application of a GFP sentinel system for the identification of RNA interference in Blastomyces dermatitidis illuminates the role of septin in morphogenesis and sporulation. Eukaryol Cell 2007 May Krajajun T, Sathapatayavongs B, Pracharktam R, Nitiyanant P, leelachaikul P, Wanachiwanawin W, Chaiprasert A, Assanasen P, Saipetch M, Mootsikapun P, Chelchotisakd P, Lekhakula A, Mitarnun W, Kalnauwakul S, Supparatpinyo K, Chaiwarith R, Chiewchavit S, Tananuvat N, Srisiri S, Suankratay C, Kulwichit W, Wongsaisuwan M, and Somkaew S. Clinical and Epidemiological Analyses of Human Phthiosis in Thailand. Clin Infect Dis 2006 Sep;43: Krajajun T, Kunakorn M, Pracharktam R, Chongtrakool P, Sathapatayavongs B, Chaiprasert A, Vanittanakom N, Chindamporn A, and Mootsikapun P. Identification of a Novel 74-kDa Immunodominant Antigen of Pythium insidiosum Recognized by Sera from Human Patients with Pythiosis. J Clin Microbiol 2006 May;44:

8 Krajajun T, Pracharktam R, Wongwaisayawan S, Rochanawutinon M, Kuankorn M, and Kunavisarut S. Ocular pythiosis: is it under-diagnosed? Am J Ophthalmol 2004 Feb;137(2): Krajajun T, Kunakorn M, Niemhom S, Chongtrakool P and Pracharktam R. Development and Evaluation of an In-House Enzyme-Linked Immunosorbent Assay for Early Diagnosis and Monitoring of Human Pythiosis. Clin Diagn Lab Immunol 2002 Mar;9(2): Krajaejun, T, E. Chansue, and R. Pracharktam A Devastating Ocular Infection Caused by Pythium insidiosum: A Case Report. Abstract book: The 40th Annual Meeting of Infectious Disease Society of America (IDSA), Chicago, Illinois-USA. 21. Krajaejun, T, R. Pracharktam, B. Sathapatayavongs, P. Nitiyanant, S. Tanrattanakorn, A. Chaiprasert, W. Wanachiwanawin, W. Manuskiatti, M. Saipetch, P. Chetchotisakd, P. Mootsikapun, S. Srisiri, C. Suankratai, L. Bhoopat, K. Supparatpinyo, W. Mitarnun, A. Lekhakula, and M. Vongsaisuwon Current Human Pythiosis in Thailand. Abstract Book: The 10th Congress of Mycology, Paris-France. 22. Krajaejun, T, M. Kunakorn, B. Sathapatayavongs, and R. Pracharktam Review of Human Pythiosis in Ramathibodi Hospital. Abstract Book: The 27th Annual Meeting of the Infectious Disease Association of Thailand, Ayuthaya- Thailand. 23. Krajaejun, T, M. Kunakorn, S. Niemhom, P. Chongtrakool, and R. Pracharktam Development and Evaluation of an In-House Enzyme-Linked Immunosorbent Assay for Early Diagnosis and Monitoring of Human Pythiosis. Clin. Diagn. Lab. Immunol. 9: