Nina R. Derby 11/12/2013 CURRICULUM VITAE

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1 CURRICULUM VITAE Nina Rafterman Derby Population Council Center for Biomedical Research 1230 York Avenue New York, NY Tel: PERSONAL DATA Born: November 22, 1978; New York, New York Citizenship: U.S.A. EDUCATION 2007 Doctor of Philosophy in Pathobiology University of Washington, Seattle, WA 2000 Bachelor of Arts degree in Biochemistry cum laude, high honors in biochemistry Smith College, Northampton, MA RESEARCH EXPERIENCE Senior Research Investigator (January 2012 present) Laboratory of Dr. Melissa Robbiani, Population Council Center for Biomedical Research, New York, NY - Evaluate the impact of poly(i:c) on dendritic cell (DC) and T cell biology and HIV/SIV infection in vitro and in vivo. - Oversee in vivo macaque studies of microbicide intravaginal rings and gels to prevent HIV infection - Supervise a research investigator and two research technicians and manage their projects (vaginal ring and gel microbicides, small molecule inhibitors of HIV, in vitro HSV-2 studies) - Increased leadership responsibility includes: greater role in intravaginal ring and gel microbicide study design, analysis, and manuscript preparation; assistance with grant writing; and presentation at meetings. Research Investigator (September 2008 December 2011) Laboratory of Dr. Melissa Robbiani, Population Council Center for Biomedical Research, New York, NY. - Investigated how poly(i:c) impacts DC biology and the implications for HIV infection of DCs and transmission to T cells using both in vitro and in vivo models. - Oversaw pre-clinical in vivo studies of microbicide intravaginal rings - Managed research technicians, contributed to grant writing, prepared manuscripts for microbicide studies, and presented at meetings. Postdoctoral Fellow (September 2007 August 2008) Laboratory of Dr. Melissa Robbiani, Population Council Center for Biomedical Research, New York, NY. - Studied how TLR ligation affects dendritic cell biology with implications for HIV infection of DCs and transmission of the virus from DCs to T cells. Investigated the mechanism by which the TLR3 ligand poly(i:c) blocked HIV replication, specifically the role of type-i IFNs and the innate intracellular HIV resistance factor, APOBEC3G. Postdoctoral Fellow (June 2007 September 2007) Laboratory of Dr. Julie Overbaugh, Fred Hutchinson Cancer Research Center, Seattle, WA. - Helped complete a study on a cohort of HIV positive female sex workers investigating how the neutralizing antibody response to HIV infection impacts the susceptibility to re-infection with a different HIV strain. This study has significant implications for the potential success or failure of antibody-based HIV vaccines. Page 1 of 5

2 Predoctoral Student ( ). Laboratory of Dr. Leonidas Stamatatos, Seattle Biomedical Research Institute, Seattle, WA. - Characterized antibody responses elicited in animals immunized with HIV Envelope (Env) proteins. Investigated how broadly reactive neutralizing antibodies develop in a macaque model of HIV infection. Research objectives included the preparation and characterization of Env proteins; design of assays to characterize the epitope specificity of antibodies in immunized and infected macaques; generation and characterization of monoclonal antibodies in immunized mice; and examination of the ability of vaccine-elicited antibodies to protect macaques from virus challenge. Research Technician ( ). Laboratories of Dr. Rick Rogers and Dr. Maria Rupnick, Department of Environmental Health, Harvard School of Public Health, and Department of Surgical Research, Children s Hospital, Boston, MA. - Studied the role of vascular remodeling and angiogenesis in the regulation of adipose tissue growth in mice as a model for tumor growth using confocal and scanning electron microscopy. - Examined particle deposition in the lungs of neonatal, juvenile, and adult rats by aerosol exposure and confocal microscopy to understand whether neonates were more susceptible than adults to alveolar particle deposition. Bachelor of Arts in Biochemistry Thesis ( ). Laboratory of Dr. Dany Adams, Departments of Biochemistry and Molecular and Cell Biology, Smith College, Northampton, MA. - Investigated the role of microtubules in cadherin-mediated cell-cell adhesion in embryonic zebrafish in vivo using microinjection, immunocytochemistry, and confocal microscopy. ACADEMIC AWARDS AND FELLOWSHIPS 2005 AIDS Vaccines Conference Student Scholarship 2005 Keystone HIV Vaccines Conference Student Scholarship 2004 Keystone HIV Vaccines Conference Student Scholarship 2004 NIH Training Grant AI renewal 2003 NIH Training Grant AI (University of Washington Department of Pathobiology) ARCS (Achievement Rewards for College Scientists) Predoctoral Fellowship Dean s List, First Group Scholar T.J. Watson Memorial College Scholarship 1999 Howard Hughes Undergraduate Summer Research Fellowship American Heart Association Undergraduate Research Fellowship 1997 National Science Foundation Research Experience for Undergraduates Fellowship TALKS 2013 A potent combination microbicide gel inhibits SHIV-RT, HSV-2, and HPV infections in vivo STD and AIDS World Congress, Vienna, Austria Characterization of the epitope specificities and neutralization properties of antibodies elicited in response to the HIV-1 envelope during immunization and infection AIDS and STD Research Symposium. University of Washington Center for AIDS and STD, Seattle, Washington Immunization with modified gp140s elicits neutralizing antibodies with narrow specificity that target distinct epitopes from those targeted by broadly neutralizing sera. AIDS Vaccines 2005 Conference, Montreal, Quebec, Canada. PUBLICATIONS 1. Kenney, J., Rodriguez, A., Kizima, L., Seidor, S., Menon, R., Jean-Pierre, N., Pugach, P., Levendosky, K., Derby, N., Gettie, A., Blanchard, J., Piatak, M., Lifson, J.D., Paglini, M.G., Zydowsky, T.M., Robbiani, M., Fernandez-Romero, J.A A potential non-arv Page 2 of 5

3 microbicide a modified zinc acetate gel is safe and effective against SHIV-RT and HSV-2 infection in vivo. Antimicrob. Agents Chemother. 57: Derby, N., Zydowsky, T., Robbiani, M In search of the optimal delivery method for anti-hiv microbicides: are intravaginal rings the way forward? Expert Rev. Anti Infect. Ther. 11: Teleshova, N., Derby, N., Martinelli, E., Pugach, P., Calenda, C., Robbiani, M Simian immunodeficiency virus interactions with macaque dendritic cells. Adv Exp Med Biol. 2013;762: *Singer, R., *Mawson, P., *Derby, N., Rodriguez, A., Kizima, L., Kenney, J., Aravantinou, M., Gettie, A., Blanchard, J., Piatak, M. Jr., Lifson, J.D., Fernández-Romero, J.A., **Robbiani, M., **Zydowsky, T.M An intravaginal ring that releases the non-nucleoside reverse transcriptase inhibitor MIV-150 prevents SHIV transmission in macaques. Sci Transl Med. 4:150. *Authors contributed equally. **Equal senior authors. 5. *Aravantinou, M., *Singer, R., *Derby, N., Calenda, G., Mawson, P., Abraham, C.J., Menon, R., Seidor, S., Goldman, D., Kenney, J., Villegas, G., Gettie, A., Blanchard, J., Lifson, J.D., Piatak, M., Fernández-Romero, J.A., Zydowsky, T.M., Teleshova, N., Robbiani, M The non-nucleoside reverse transcriptase inhibitor MIV-160 delivered from an intravaginal ring, but not from a carrageenan gel, protects against simian/human immunodeficiency virus-rt infection. AIDS Res Hum Retroviruses. *Authors contributed equally. 6. *Kenney, J., *Singer, R., *Derby, N., Aravantinou, M., Abraham, C.J., Menon, R., Seidor, S., Zhang, S., Gettie, A., Blanchard, J., Piatak, M. Jr., Lifson, J.D., Fernández-Romero, J.A., Zydowsky, T.M., Robbiani, M A single dose of a MIV-150/zinc acetate gel provides 24 h of protection against vaginal simian human immunodeficiency virus reverse transcriptase infection, with more limited protection rectally 8-24h after gel use. AIDS Res Hum Retroviruses. *Authors contributed equally. 7. Derby, N., Martinelli, E., Robbiani, M Myeloid dendritic cells in HIV-1 infection. Curr. Opin. HIV AIDS. 6: *Singer, R., *Derby, N., Rodriguez, A., Kizima, L., Kenney, J., Aravantinou, M., Chudolij, A., Gettie, A., Blanchard, J., Lifson, J.D., Piatak, M. Jr., Fernández-Romero, J.A., Zydowsky, T.M., Robbiani, M , The non-nucleoside reverse transcriptase inhibitor MIV-150 in carrageenan gel prevents rectal transmission of simian human immunodeficiency virus infection in macaques. J. Virol. 85:5504. *Authors contributed equally. 9. *Trapp, S., *Derby, N.R., Singer, R., Shaw, A., Williams, V.G., Turville, S.G., Bess Jr., J.W., Lifson, J.D., Robbiani, M The double-stranded RNA analog poly(i:c) inhibits HIV amplification in dendritic cells via type I IFN-mediated expression of active APOBEC3G. J. Virol. 83:884. * Authors contributed equally. 10. Blish, C.A., Dogan, O.C., Derby, N.R., Nguyen, M.A., Chohan, B., Richardson, B., Overbaugh, J Human immunodeficiency virus type 1 superinfection occurs despite relatively robust neutralizing antibody responses. J. Virol. 82: Derby, N.R., Gray, S., Wayner, E., Campogan, D., Vlachogiannis, G., Kraft, Z., Barnett, S.W., Srivastava, I.K., Stamatatos, L Isolation and characterization of monoclonal antibodies elicited by trimeric HIV-1 Env gp140 proteins. Virology. 366: *Kraft, Z., *Derby, N.R., McCaffrey, R.A., Niec, R., Blay, W., Haigwood, N.L., Moysi, E., Saunders, C.J., Wrin, T., Petropoulos, C.J., McElrath, J.M., Stamatatos, L Macaques infected with a CCR5-tropic Simian/Human Immunodeficiency Virus develop broadly reactive anti-hiv neutralizing antibodies. J. Virol. 81:6402. *Authors contributed equally. Page 3 of 5

4 13. Derby, N.R., Kraft, Z., Kan, E., Crooks, E.T., Barnett, S.W., Srivastava, I.K., Binley, J.M., Stamatatos, L.S Antibody responses elicited in macaques immunized with Human Immunodeficiency Virus type 1 (HIV-1) SF162-derived gp140 Envelope immunogens: comparison with those elicited during homologous Simian/Human Immunodeficiency Virus SHIV SF162P4 and heterologous HIV-1 infection. J. Virol. 80: Burke, B., Derby, N.R., Kraft, Z., Saunders, C.J., Llewellyn, N., Zharkikh, I., Vojtech, L., Zhu, T., Srivastava, I.K., Barnett, S.W., Stamatatos, L Viral evolution in macaques coinfected with CCR5- and CXCR4-tropic SHIVs in the presence or absence of vaccineelicited anti-ccr5 SHIV neutralizing antibodies. Virology. 355:138. ABSTRACTS AND THESES 1. Rodriguez, A., Mizenina, O., Kizima, L., Levendosky, K., Kleinbeck, K., Derby, N., Robbiani, M., Herold, B., Zydowsky, T., Fernandez Romero, J.A In vitro and in vivo evaluation of carrageenan-based formulations to prevent HPV acquisition. Poster STD and AIDS World Congress. Vienna, Austria. 2. Kenney, J., Derby, N., Aravantinou, M., Rana, S., Lifson, J.D., Piatak, Jr., M., Gettie, A., Blanchard, J., Robbiani, M. Poster STD and AIDS World Congress. Vienna, Austria. 3. *Singer, R., *Mawson, P., *Derby, N., Rodriguez, A., Kizima, L., Kenney, J., Aravantinou, M., Gettie, A., Blanchard, J., Piatak, M. Jr., Lifson, J.D., Fernández-Romero, J.A., Robbiani, M., Zydowsky, T.M An intravaginal ring (IVR) that releases the NNRTI MIV-150 prevents SHIV transmission in macaques. Oral. International Microbicides 2012 Conference. Sydney, Australia. *Authors contributed equally. 4. *Kenney, J., *Singer, R., *Derby, N., Gettie, A., Blanchard, J., Lifson, J.D., Piatak, M. Jr., Fernández-Romero, J.A., Zydowsky, T., Robbiani, M A single dose of the prototype MIV-150/ZA/CG gel provides 24 hours of protection against vaginal SHIV-RT infection, with limited protection rectally 8-24 hours after gel use. Short Oral. International Microbicides 2012 Conference. Sydney, Australia. *Authors contributed equally. 5. *Aravantinou, M., *Singer, R., *Derby, N., Calenda, G., Mawson, P., Abraham, C., Menon, R., Seidor, S., Kenney, J., Villegas, G., Gettie, A., Blanchard, J., Piatak, M. Jr., Lifson, J.D., Fernández-Romero, J.A., Zydowsky, T.M., Teleshova, N., Robbiani, M Prevention of vaginal SHIV-RT infection of macaques by the NNRTI MIV-160 formulated in a ring vs. a carrageenan gel. Oral. International Microbicides 2012 Conference. Sydney, Australia. *Authors contributed equally. 6. Derby, N., Singer, R., Tharinger, H., Gettie, A., Blanchard, J., Lifson, J.D., Piatak, M. Jr., Slazar, A., Robbiani, M Impact of poly(ic) on DC activation and HIV/SIV replication in vitro and in vivo. Poster Conference on Retroviruses and Opportunistic Infections. Seattle, Washington. 7. Derby, N., Trapp, S., Singer, R., Shaw, A., Williams, V.G., Turville, S.G., Bess, Jr., J.W., Lifson, J.D., Robbiani, M Double-stranded RNA analog poly(i:c) inhibits HIV amplification in dendritic cells via type I IFN-mediated activation of APOBEC3G. Poster. Keystone Dendritic Cells/Immune Pattern Recognition 2009 Conference, Banff, Alberta, Canada. 8. Derby, N Designing immunogens to elicit broadly reactive neutralizing antibodies to the HIV Envelope. Ph.D. Dissertation. Department of Pathobiology, University of Washington, Seattle, Washington. Page 4 of 5

5 9. Derby, N., Wayner, E., Campogan, D., Barnett, S.W., Srivastava, I.K., Kraft, Z., Stamatatos, L Characterization of monoclonal antibodies from mice immunized with HIV envelope immunogens. Poster. Keystone HIV Vaccines 2005 Conference, Banff, Alberta, Canada. 10. Derby, N.R., McCaffrey, R., Saunders, C.J., Stamatatos, L Design and characterization of gp140 immunogens derived from the SF162 and V2 Envelopes lacking the V3 loop. Poster. Keystone HIV Vaccines 2004 Conference, Whistler, British Columbia, Canada. 11. Derby, N.R., McCaffrey, R., Saunders, C.J., Stamatatos, L Generation and structural characterization of novel gp140 immunogens derived from the SF162 and V2 Envelopes lacking the crown of the V3 loop. Poster. Non-Human Primate Models for AIDS 2003 Conference, Seattle, Washington. 12. McCaffrey, R., Saunders, C.J., Derby, N.R., Stamatatos, L Role of clade B and clade A Envelope glycosylation in HIV-1 tropism, transmission, and neutralization susceptibility. Poster. Non-Human Primate Models for AIDS 2002 Conference, Seattle, Washington. 13. Derby, N.R., Rupnick, M.A., Lai, J., Roberts, M., Dikie, R., Nayak, R., Rogers, R.A Pericyte distribution and microvessel architecture in adipose tissue from normal (C57BL/6) and leptin-deficient (ob/ob) mice. Poster. New England Society for Microscopy 2001 Meeting, Woods Hole, Massachusetts. 14. Derby, N.R Microtubules are necessary for cadherin-mediated cell-cell adhesion in Danio rerio in vivo. Undergraduate Thesis. Smith College, Northampton, Massachusetts. PATENTS 2002 McCaffrey, R.A., Derby, N.R., Stamatatos, L. Altering the immunogenicity of the HIV envelope by introducing specific amino acid deletions within the third hypervariable region (V3 loop) of the HIV envelope glycoprotein. University of Washington, Seattle, Washington. PROFESSIONAL ORGANIZATIONS AND COMMITTEES 2006-present Member, AAAS (American Association for the Advancement of Science) 2000-present Member, Sigma Xi Page 5 of 5