, , 2. ment of Physiology, Henan Medical U niversity, Zhengzhou

Size: px

Start display at page:

Download ", , 2. ment of Physiology, Henan Medical U niversity, Zhengzhou"

Drusilla McGee
5 years ago
Views:

73 (, 00083, ) :,,, ; : () 30min,, 0 ;, ; 4h ; (), ; : ;,, ; ; ; ; IMPROVED SCORING METHOD FOR ARTHRITIC FL EXION PAIN TEST IN FRE UND S ADJUVANT MONOARTHRITIS MODEL IN THE RAT L IU HongXiang, XION G

1 73 (, 00083, ) :,,, ; : () 30min,, 0 ;, ; 4h ; (), ; : ;,, ; ; ; ; IMPROVED SCORING METHOD FOR ARTHRITIC FL EXION PAIN TEST IN FRE UND S ADJUVANT MONOARTHRITIS MODEL IN THE RAT L IU HongXiang, XION G Liang, L UO Fei, HAN JiSheng Neuroscience Research Center, Beijing Medical University, Beijing 00083, Depart ment of Physiology, Henan Medical U niversity, Zhengzhou Abstract Aim : Squeaking response has long been regarded as a specific manifestation of the perception of pain in animals and used as the end point of scores of arthritic flex ion pain test in rats with ajuvantinduced arthritis. However, we found that the squeak ing response of rats was not as stable as the legwithdrawal response and showed a larger individual variation. In t he present st udy, we systemically observed t he stability of t he legwit hdrawal scores of art hritic flexion pain test in adjuvantinduced monoart hritic rat s and their sensitivity to analgesic drugs. Results : In normal rats when the ankle joints were bent gently, very few legwit hdrawal responses occured. This score increased sig nificantly in inflamed joint s. The legwit hdrawal scores in inflammed legs kept stable for several hours in a single experiment. Morp hine and indomet hacin produced a dosede pendent reduction of the scores. The results showed that the legwithdrawal response DA China Academic Journal Electronic Publishing House. All rights reserved.

2 74 can also be used as a reliable index for the arthritic pain. However, comparative study of the results obtained by squeaking alone, legwithdrawal alone and the total scores of squeaking and legwithdrawal revealed that it seems more reliable to use the total scores as the end point of arthritic flexion pain. Keywords Squeaking ; Legwithdrawal ; Arthritic flexion pain test ; Monoarthritis ; morphine ; indomethacin ; rat cm, cm,, ml,, :,, / /, (Morphine Hydrochloride), [6 ],, mg/ ml ( Indomethacin) Sigma,, 5 %NaHCO3, g/ ml,. CFA 0 % (300mg/ kg,i. p. ),,75 %, Wistar, 50, 80g 46,,, 5mm, 0. 03ml (CFA) 3. 9, 0,, 30min (). ( ), 5s (CFA) :(),5 (ml 0. 85ml 0. 5ml ; Arlacel A, Gibco ) () 0, (0mg/ ml, 05 ), 00 () 8 ml, ( 6cm 0. 6mm), China Academic Journal Electronic Publishing House. All rights reserved.

3 75 4.,,, P < 0. 05,., () 4 5cm,,ml/ 0 CFA 00g ,, 0. 5,, ml/ 00g, , : Kruskal 4 Wallis ; ( n > 30),, (ANOVA) Duncan s ( P > 0. 05) 4h Table Legwithdra wal scores of arthritic flexion pain test for the ankle joints within 4 hours after putting the rat in the holder mode of flexion Normal side Plantar flexion Inflamed side Normal side Dorsal flexion Inflamed side n ( ) Time (hours after fixation) : 3 P < 0. 0 Note :numerals in this table are legwithdrawal scores of arthritic flexion pain test, mean SE. ankle joint 3 3 P < 0. 0 compared with normal () %34. 6 %,,5 : ; , 9 ; , 50 % ; ; 8. 3 %46. % (3) 0., , 70 CFA China Academic Journal Electronic Publishing House. All rights reserved.

4 76 4, 68 NaHCO 3 ) 3 30min,, ( KruskalWallis, P < 0. 05), 0. 5,,,,4,8mg/ kg,, ( 5 % Table Comparison among the squeaking, withdra wal and total scores of arthritic flexion pain test for the in flamed ankles in adjuvantinduced monoarthritic rats mode of flexion scoring 0 (59) (93) (94) ( ) Time in weeks after of CFA (n) 3 (9) 4 (79) 5 (89) 6 (50) 7 (63) 8 (49) 9 (63) Squeaking 0. 0 ( %) Withdrawal ( %) Plantar flexion Total 0. 9 ( %) Squeaking ( %) Withdrawal ( %) Dorsal flexion Total 0. 6 ( %) , 9 0. ( () 9 ) ( ), 5, 0., 8, ; (39 ),,5. 03,. 6, ,, () 3, 50 %, China Academic Journal Electronic Publishing House. All rights reserved.

5 77 37 %, ( 9 ) 50 %, 50 %) %, (, 3 Table 3 Influences of indomethacin and morphine on the legwithdra wal scores of arthitic flexion pain test for in flamed ankle joint in monoarthritic rats Indices Drugs Dose (mg/ kg) Maximal reduction produced by drugs Plantar flexion Dorsal flexion Indomethacin Morphine Indomethacin Morphine 5 %NaHCO NS %NaHCO P < 0. 05, P < 0. 00,, 3 P < 0. 05, P < 0. 00,compared with control # P < 0. 05,, # P < 0. 05,compared with the smallest dosage NS # ,,,,, 950 LaBelle Tislow, [7 ], [4,9 ],, : () [8 ],,, (),,, China Academic Journal Electronic Publishing House. All rights reserved.

6 78 of adjuvantinduced art hritis in t he rat : 30min, : possible relationship withchronic pain., 0 ; Behav Brain Res, 987, 4 9., 5 Braga PC, Dalsasso M, Bemini A, et al. ; Antinociceptive activity of toninelect rop hysiological correlates in a : rat chronic pain model. Neurosci Lett, 993, Calvino B, Couraud J Y, Besson J M. Pre, vaccination wit h diluted Freund adjuvant, prevent s t he develop ment of chronic pain, and t ransient release of cerebrospinal fluid in rats. Pain, 994, [ ] 7 Winter CA, Kling PJ, Tocco DJ, Tanabe, K. Analgesic activity of diflunisal in rat s [7, ] with hyperalgesia induced by Freund s ad, juvant. J Pharmacol Exp Ther, 979, , 8,,.,.,996, 38. LaBelle A, Tislow R. A method of evalu ating analgesics of t he antiart hralgic type in t he laboratory animal. J Pharmacol Exp Ther, 950, Kuzuna D, Kawai K. Evaluation of anal gesic agents in rats with adjuvant arthri tis. Chem Pharm Bull, 975, Capetola RJ, Shriver DA, Rosenthale M E. Activity of a new nonnarcotic anal gesic, suprofen, on pat hologicinduced pain in t he rat. Pharmacologist, 978, Calvino B, CreponBernard MO, LeBars D. Parallel clinical and behavioural st udies salmon calci substance P in adjuvantinduced art hritis 9,,,..,996, Randall LO and Selitto JJ. A method for measurement of analgesic activity on in flamed tissue. Arch Int Pharmacodyn, 957, Hirose K, J yoyama H. Measurement of arthritic pain and effects of analgesics in t he adjuvantt reated rat. J apan J Phar macol, 97, Winter CA and Flataker L. Reaction t hresholds to pressure in edematous hindpaws of rat s and responses to anal gesic drugs. J Pharmacol Exp Ther, 965, China Academic Journal Electronic Publishing House. All rights reserved.

Japan. J. Pharmacol. 34, (1984) 67

Japan. J. Pharmacol. 34, (1984) 67 Japan. J. Pharmacol. 34, 67-77 (1984) 67 Inhibition of Electrically-Induced Vocalization in Adjuvant Arthritic Rats as a Novel Method for Evaluating Analgesic Drugs Shigeru OKUYAMA and Hironaka AIHARA