Paper-based skin patch for the diagnostic screening of cystic fibrosis

Transcription

1 Electronic Supplementry Mteril (ESI) for ChemComm. This journl is The Royl Society of Chemistry 2015 Supplementry informtion Pper-bsed skin ptch for the dignostic screening of cystic fibrosis Xun Mu,* Xiolei Xin, Chengyn Fn, Xue Li, b Xinlun Tin,* b Ki-Feng Xu b nd Zhi Zheng* 1 Institute of Bsic Medicl Sciences Chinese Acdemy of Medicl Sciences School of Bsic Medicine Peking Union Medicl College, 5 Dongdn Sntio, , Beijing, Chin. Tel: ; E-mil: mux@ibms.pumc.edu.cn; Fx/Tel: ; E-mil: zhizheng10@yhoo.com; 2 Deprtment of Respirtory Medicine, Peking Union Medicl College Hospitl, Chinese Acdemy of Medicl Sciences nd Peking Union Medicl College, , Beijing, Chin. Fx/Tel: ; E-mil: xinlun_t@sin.com. Mterils nd Instruments DE 81 Ion exchnge pper nd Chr 1 pper (Whtmn, GE, US); 1624W Tegderm nd Scotch tpe (3M, US); Pilocrpine Nitrte (meets USP test specifictions), Sodium Chloride, Sodium L- lctte, nd Sodium Bicrbonte (Sigm-Aldrich, US); ph test pper with rnge of 1 to 14 (Hngzhou Specil Pper Co., Ltd, Chin); GC-YS-107 Wter Sensitive Ink (Gocolor S.Z. Chemicl Co., Ltd, Chin); MK40 Softbox for providing controlled light source (Shentu, Chin); ABL850 Anlyzer (Rdiometer, Dnmrk); Hole Punchers with the size of 2 mm, 4 mm nd 5 mm; Smsung GT-I9300; Cnon Powershot A700.

2 Figure S1. The cross-sectionl schemtics nd imges of three sections of the pper-bsed skin ptch. The imges lso show prt of holes on the dhesive tpe. Figure S2. The schemtics show the rnge of the ph test pper. The blue rrow indictes the color chnge of the section 2 of the skin ptch. Figure S3. The men intensity of yellow color (in CMYK) of the section 1 of three skin ptches in the controlled light box. The color intensity shows high consistency with CV of 0.5%, demonstrting controlled light source. The dsh line indictes the verge intensity.

3 Figure S4. The different operting procedures of stndrd dignostic methods nd the pper-bsed skin ptch. The stndrd methods hve multiple steps involving lenghy collection nd troublesome liquid trnsfering. On the contrry, the skin ptch hs stremlined nd integrted steps, showing more efficiency nd less risk of evpertion errors. Figure S5. Triplicte experiments of detecting chloride solution (1.5 µl, 100 mm) on the skin ptch. The coefficient vrition is bout 9.6%. Figure S6. The cholorimetric results of mesuring volume of differet nion solutions (1.5µL, 10 mm) on the section 3 of the skin ptch. Becuse the hydrochromic ink is specificlly triggered by wter, the mesured volume seems not to be influnced by nions. The coefficient vrition is bout 13.8% tht is similr to the overll vritions of color (mgent) detection.

4 Tble S1. Technicl comprison of the skin ptch nd the reference methods Pper-bsed Skin Ptch Reference methods Gibson-Cooke Wescor Mcroduct QPIT Swet-Chek system Anlyticl Trgets Anions Chloride Anions Detection mechnism Colorimetric Coulometry Conductivity Overll Coefficient of Assy Time (min) Vrition ~ 15% < 5% < 2 % b Coefficient of Determintion c NA Dynmic Rnge 0 to 140 mm (equivlent NCl) Stimultion 5 Collection Detection ~ 5 30 to 900 mm 0 to 150 mmol/l (equivlent NCl) b 38 to 95 d 38 to 71 d 22 d ; 20 d ; Cost (US $) ~ 0.62 f 17 e 32 e Product dt sheet of ABL 850L; b Product dt sheet of Swet-Chek Anlyzer; c Stndrd chloride solutions in Fig. 2. d J Peditr (Rio J), 2010, 86(2): ; e Clin. Biochem., 2009, 42: ; f Detils in Tble S2; Tble S2. Shelf life nd mteril cost of the skin ptch Shelf life Quntity Cost For one skin ptch (US $) Quntity Cost (US $) Tegderm 1624W 3 yers One piece 0.5 One piece 0.5 Hydrochromic > 12 months ink below 30 C 25 ml µl Anion exchnge One-sixth NA 400 pieces Pper piece Adhesive Tpe NA 32.9 meter mm Chr 1 pper NA 100 meter mm ph test Pper (1- One-third NA 80 pieces ) piece Negligible Sum 1 yer ~ 0.62 Product dt sheets.

5 Tble S 3. Clinicl comprison of the skin ptch nd the reference method Ptients Age Gender Skin ptch (80 mm cutoff) Clinicl Dignosis QPIT (60 mm cutoff) Dignostic Accurcy A 16 Femle Non-CF Non-CF Consistency B 31 Femle Non-CF Non-CF Consistency C 40 Femle Non-CF Non-CF Consistency D 29 Mle Non-CF Non-CF Consistency E 14 Mle Non-CF Non-CF Consistency F 39 Femle Non-CF Non-CF Consistency G 11 Mle CF CF Consistency H 10 Femle CF CF Consistency Respirology, 2015, 20, 312. Tble S4. Recovery of the instrumentl nlyzer for detecting chloride Chloride Concentrion (mm) Recovery (%) 0 b ABL 850L; b Not detecble