AAFCO Proficiency Testing Program: 2016 Participation
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1 AAFCO Proficiency Testing Program: 206 Participation
2 Lamb Feed, Medicated Swine Feed, Medicated Poultry/Game Bird Feed Dairy Beef Feed Cattle Mineral Molasses Product, Dehydrated Cat Food, Dry Llama Feed Swine Feed, Medicated Goat Feed Poultry Feed, Medicated Lamb Feed, Medicated Cattle Feed Supplement Animal Feed Scheme # Labs
3 Proximates AFS Analyte Participation: Median Labs Per Sample Urea (%) Loss on Drying (%) Protein (%) Crude Fat (%) Crude Fiber (%) Ash (%) Total sugars (%) Urease (%) Fiber, Acid Detergent (%) Fiber, Neutral Detergent (%) Moisture (%) Loss on Drying, High Temp. (%) Starch (%) Fat, Acid hydrolysis (%) Fiber, total dietary TDF (%) Water activity (Units) Non Protein N (NPN) (%) Peroxide value (meq/kg)
4 Minerals AFS Analyte Participation: Median Labs Per Sample Aluminum (mg / kg (ppm)) Boron (mg / kg (ppm)) Calcium (%) Cobalt (mg / kg (ppm)) Copper (mg / kg (ppm)) Fluorine (mg / kg (ppm)) Iodine (mg / kg (ppm)) Iron (mg / kg (ppm)) Magnesium (%) Manganese (mg / kg (ppm)) Phosphorus (%) Potassium (%) Salt as chloride (%) Selenium (mg / kg (ppm)) Sodium (%) Sulfur (%) Zinc (mg / kg (ppm)) Molybdenum (mg / kg (ppm)) Barium (mg / kg (ppm)) Vanadium (mg / kg (ppm)) Chloride (%) Arsenic, total (mg / kg (ppm)) Cadmium (mg / kg (ppm)) Chromium (mg / kg (ppm)) Lead (mg / kg (ppm)) Mercury (µg / kg (ppb)) Nickel (mg / kg (ppm))
5 Vet Drugs Sub Scheme Amprolium (mg / kg (ppm)) Carbadox (mg / kg (ppm)) Chlortetracycline (mg / kg (ppm)) Decoquinate (mg / kg (ppm)) Ethoxyquin (mg / kg (ppm)) Hygromycin B (Units / kg) Lasalocid Sodium (mg / kg (ppm)) Melengestrol Acetate (mg / kg (ppm)) Monensin (mg / kg (ppm)) Nicarbazin (mg / kg (ppm)) Oxytetracycline (mg / kg (ppm)) Pyrantel Tartrate (mg / kg (ppm)) Salinomycin (mg / kg (ppm)) Tiamulin (mg / kg (ppm)) Tylosin (mg / kg (ppm)) Narasin (mg / kg (ppm)) Fenbendazole (mg / kg (ppm)) Ractopamine Hydrochloride (mg / kg (ppm)) Methoprene (mg /kg (ppm)) Carbadox, residual (µg / kg) Chlortetracycline, residual (µg / kg) Decoquinate, residual (µg / kg) Lasalocid sodium, residual (µg / kg) Melengestrol Acetate, residual (µg / kg) Monensin, residual (µg / kg) Oxytetracycline, residual (µg / kg) Pyrantel Tartrate, residual (µg / kg) Fenbendazole, residual (µg / kg) Ractopamine Hydrochloride, residual (µg / kg) AFS Analyte Participation: Median Labs Per Sample Values Submitted
6 AFS Analyte Participation: Median Labs Per Sample Vitamins Choline Chloride (mg / kg (ppm)) Niacin (mg / kg (ppm)) Pantothenic Acid (mg / kg (ppm)) Riboflavin (mg / kg (ppm)) Thiamine (mg / kg (ppm)) Vitamin A (KU / kg) Vitamin B2 (µg / kg (ppb)) Vitamin D3 (KU / kg) Vitamin E (IU/kg) Vitamin C, phosphorylated (mg / kg (ppm)) Pyridoxine (µg / g) Folic Acid (mg / kg (ppm)) Biotin (mg / kg (ppm))
7 Amino Acids AFS Analyte Participation: Median Labs Per Sample Alanine (%) Arginine (%) Aspartic (%) Cysteine/Cystine (%) Glutamic (%) Glycine (%) Histidine (%) Isoleucine (%) Leucine (%) L-Lysine (%) Methionine (%) Phenylalanine (%) Proline (%) Serine (%) Threonine (%) Tryptophan (%) Tyrosine (%) Valine (%) Taurine (%)
8 Sugars AFS Analyte Participation: Median Labs Per Sample Fructose (%) Galactose (%) Glucose (%) Lactose (%) Maltose (%) Sucrose (%) Raffinose (%) Stachyose (%)
9 Sample # Animal Feed Scheme 206 Z-Cut Homogeneity Screen Sample Name Pass Z-Cut %RSD % Protein # in Z-Cut 2062 Cattle Feed Supplement 0.00% Lamb Feed, Medicated 0.00% Poultry Feed, Medicated 0.5% Goat Feed 0.00% Swine Feed, Medicated 0.00% Llama Feed.6% Cat Food, Dry 0.52% Molasses Product, Dehydrated 3.4% Cattle Mineral.5% Dairy Beef Feed 0.38% Poultry/Game Bird Feed 0.00% Swine Feed, Medicated 0.00% Lamb Feed, Medicated - - -
10 # Labs Pet Food Scheme Participation
11 Proximates PFS Analyte Participation: Median Labs Per Sample Loss on Drying (%) 5 Protein (%) 55 Crude Fat (%) Crude Fiber (%) Ash (%) 42 Total sugars (%) 4 Fiber, Acid Detergent (%) 8 Fiber, Neutral Detergent (%) Moisture (%) 9 Loss on Drying, High Temp. (%) 25 Starch (%) 6 Fat, Acid hydrolysis (%) Fiber, total dietary TDF (%) Water activity (Units) Total Saturated Fatty Acids (% (w/w)) 4 30 The Unknown Scheme
12 Minerals Aluminum (mg / kg (ppm)) Boron (mg / kg (ppm)) Calcium (%) Cobalt (mg / kg (ppm)) Copper (mg / kg (ppm)) Iodine (mg / kg (ppm)) Iron (mg / kg (ppm)) Magnesium (%) Manganese (mg / kg (ppm)) Phosphorus (%) Potassium (%) Salt as chloride (%) Selenium (mg / kg (ppm)) Sodium (%) Sulfur (%) Zinc (mg / kg (ppm)) Molybdenum (mg / kg (ppm)) Barium (mg / kg (ppm)) Vanadium (mg / kg (ppm)) Chloride (%) Arsenic, total (mg / kg (ppm)) Cadmium (mg / kg (ppm)) Chromium (mg / kg (ppm)) Lead (mg / kg (ppm)) Mercury (µg / kg (ppb)) Nickel (mg / kg (ppm)) PFS Analyte Participation: Median Labs Per Sample Samples Barley Sample Name 2064 Tomato Pomace Lamb Meal Cheese Powder
13 NO VET DRUGS! But they are available in the DRW!!!
14 Vitamins PFS Analyte Participation: Median Labs Per Sample Choline Chloride (mg / kg (ppm)) Niacin (mg / kg (ppm)) Pantothenic Acid (mg / kg (ppm)) Riboflavin (mg / kg (ppm)) Thiamine (mg / kg (ppm)) Vitamin A (KU / kg) Vitamin B2 (µg / kg (ppb)) Vitamin D3 (KU / kg) Vitamin E (IU/kg) Pyridoxine (µg / g) Folic Acid (mg / kg (ppm)) Biotin (mg / kg (ppm))
15 Amino Acids PFS Analyte Participation: Median Labs Per Sample Alanine (%) Arginine (%) Aspartic (%) Cysteine/Cystine (%) Glutamic (%) Glycine (%) Histidine (%) Isoleucine (%) Leucine (%) L-Lysine (%) Methionine (%) Phenylalanine (%) Proline (%) Serine (%) Threonine (%) Tryptophan (%) Tyrosine (%) Valine (%) Taurine (%)
16 Sugars PFS Analyte Participation: Median Labs Per Sample 0 2 Fructose (%) Galactose (%) Glucose (%) Lactose (%) Maltose (%) Sucrose (%)
17 Pet Food Scheme Z-Cut Homogeneity Screen Sample # Sample Name Pass Z-Cut %RSD % Protein # in Z-Cut Barley 3.4% Tomato Pomace 0.5% Lamb Meal 0.00% Cheese Powder.38% Oat Flour - - -
18 Z-Score Indications in Animal Feed Scheme and Pet Food Scheme 206 to Date: 34,246 Tests Compliance 2 > Z > -2 Warning Actionable 3 < Z < % 4.6% 5.2%
19 Trilogy sources the base feeds. Mycotoxin Scheme Sample Engineering Materials thoroughly processed with regard to particle size and blending. 0,000 lb Masters Collection Carefully sourced feeds naturally contaminated with Mycotoxins. Incubated to raise concentration. Homogenous Samples for distribution. Certificate of Analysis and homogeneity data.
20 # Labs Mycotoxins Scheme Participation
21 Mycotoxin Analyte Participation All Labs Participating
22 Minerals Scheme Sample Engineering Base feed sourced from prior Check Samples. Minerals sourced separately. Able Laboratory Inc. Bob Kieffer Existing Minerals in base feed established during prior testing round. Added Minerals solubilized and sprayed over existing pre-ground base feed prior to blending and sample portioning. Spike concentrations calculated. Homogenous Samples for distribution.
23 # Labs Minerals Scheme Participation
24 Minerals Scheme Analyte Participation All Labs Participating
25 Method Codes Fatty Acids The Unknown Scheme Code Fatty Acid Groupings 54 Total Omega-3 Polyunsaturated Fatty Acids 56 Total Omega-6 Polyunsaturated Fatty Acids 58 Total Saturated Fatty Acids 62 Total Monounsaturated Fatty Acids 64 Total cis Monounsaturated Fatty Acids 66 Total Polyunsaturated Fatty Acids 68 Total cis Polyunsaturated Fatty Acids 0 Total Fat (equivalent to NLEA) 2 Total Fatty Acids Methods ##.0 Direct Methylation by Alkali Hydrolysis & GC ##.02 Direct Methylation by Acid-Alkali Hydrolysis & GC ##.99 Miscellaneous Code Individual Fatty Acids 0 Propionic Acid (3:0) 02 Butyric Acid (4:0) 03 Valeric Acid (5:0) 04 Caproic Acid (6:0) 06 Caprylic acid (8:0) 08 Capric acid (0:0) 0 Lauric Acid (2:0) 4 Myristic Acid (4:0 ) 6 Palmitic Acid (6:0) 8 Palmitoleic Acid (9c-6:) 20 Margaric acid (:0) 22 Stearic Acid (8:0) 24 Oleic Acid (9c-8:) 26 Linoleic Acid n-6 (8:2, all cis) 28 alpha-linolenic Acid n-3 (8:3, all cis) 30 Arachidic Acid (20:0) 32 Gondoic Acid (c-20:) 36 Arachidonic Acid n-6 (20:4, all cis) 38 Mead Acid n-9 (20:3, all cis) 40 Eicosapentaenoic Acid n-3 EPA (all cis) 42 Behenic Acid (22:0) 44 Erucic Acid (3c-22:) 46 Docosapentaenoic Acid n-3 DPA (all cis) 48 Lignoceric Acid (24:0) 50 Docosahexaenoic Acid n-3 DHA (all cis) 52 Nervonic Acid (24:) isomers
26 AAFCO Proficiency Testing Program Scheme Participation Animal Feed Scheme Proximates 3 % Minerals 44 % Vet Drugs % Vitamins 3 % Amino Acids 4 % Sugars % Pet Food Scheme Proximates 34 % Minerals 45 % Vitamins 4 % Amino Acids 5 % Sugars 2 % Mycotoxin Scheme Engineered Concentrations Minerals Scheme Engineered Concentrations
27 Questions?
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