Topics. Protein Bioinformatics ( ) How a Mass Spectrometers Measure Mass. Lecture 9: Quantitative Proteomics Tuesday, April 27, 2010
|
|
- Jade Lamb
- 6 years ago
- Views:
Transcription
1 Protein Bioinformatics (26.655) Lecture 9: Quantitative Proteomics Tuesday, April 27, 21 Robert N. Cole, Ph.D. Mass Spectrometry and Proteomics Facility Johns Hopkins School of Medicine 371 Broadway Research Bldg 733 N. Broadway St. Baltimore, MD 2125 Ph: (41) Topics Protein identification by mass spectrometry Relative quantification of proteins Applications How a Mass Spectrometers Measure Mass Ionization Ion Sorting Detection Ion Source Form Ions Inlet Solid, Liquid, or Vapor Mass Analyzer Sort by mass-to-charge ratio (m/z) Counts Ion Detector Spectrum m/z 1
2 Identifying Proteins by Mass Spectrometry Three Methods Bottom Up: Proteins identified from a few peptides Know cleavage site, peptide mass and sequence Do not know proteins actual size and modifications Problems: Sequence homology, seeing all peptides Top Down: Proteins identified from the intact protein Know protein s size and whether it is modified Sequence usually from ends of protein Problems: Size limitations, Internal sequences Middle Down: Proteins identified from large sections of protein Identifying Proteins from Peptide Sequence (Many proteins in solution, gel bands or spots) Nanospray Ionization Full MS Scan Parent Mass Selection Parent Mass Fragmentation Fragment Mass Scan HPLC Survey Scan Collision induced MS/MS or Tandem MS Scan Intensity Intensity m/z m/z Tandem MS Sequencing Peptides by Fragmentation b R 1 R 2 R 3 H + + H 3 N-CH-CO NH-CH-CO NH-CH-COOH y b-ion (b1) y-ion (y2) R 1 + H 3 N-CH-CO R 2 R 3 H + NH-CH-CO NH-CH-COOH 2
3 Survey (Full MS or MS1) Scan of Peptides Intensi ty Mass (m/z) MS/MS (MS2) Scan of Peptide Fragments showing Amino Acid Sequence Fragmentation of Ion ity Intens Mass (m/z) 2 15 b-ions T G P N L H G L F G R y-ions y y Fragmentation of Ion ity Intens 1 y3 y4 y7 5 y1 y y y y Mass (m/z) 3
4 Tandem MS (MS/MS) Uses Peptide Mass and Sequence Tag Need Only One Peptide Mass with >3 Amino Acids Masses in Sequence (at least two preferred) High Sample Complexity Tolerated Protein modifications identified and mapped to an amino acid High mass accuracy nice, but not required Search Engines for Protein Identification from MS Data Summary of Programs Proteome Software ExPASy expasy.proteome.org.au Free Programs ProteinProspector XProteo Prowl Mascot prospector.ucsf.edu xproteo.com:2698 prowl.rockefeller.edu rockefeller edu (Free < 3 ions) Open Source Programs OMSSA pubchem.ncbi.nlm.nih.gov/omssa/ X! Tandem Commerical Programs Mascot Sequest Spectrum Mill Proteolynx fields.scripps.edu/sequest/ Quantitative Proteomics Quantifying Individual Proteins in Complex Mixtures (Functional Proteomics) Quantifying a Proteome Reveals Changes in protein levels (biomarkers, pathways, binding partners) ) Changes in protein modifications (structure/function) Changes in subcellular localization (trafficking) Kinetics (protein turnover, modification dynamics) 4
5 Approach Non-Labeling Labeling Chemical Metabolic Quantitative Proteomics Methods Methods (Gel or MS based) Gel matching, Densitometry, Spectral Counting, Peak Intensity, Multiple Reaction Monitoring (MRM) Difference Gel Electrophoresis (DIGE) Isobaric Tags for Relative and Absolute Quantitation (itraq) Isotope-Coded Affinity Tags (ICAT) Enzymatic 18 O-Labeling Spiking Radiolabeling Stable Isotope Labeling of Amino Acids in Cell Culture (SILAC) Absolute Quantification (AQUA) Multiple Reaction Monitoring (MRM) Quantification Concatamers (QconCAT) Best Approach? There is NO one best approach. All approaches are: Complementary different separation techniques different sets of proteins identified Technically challenging sample preparation data acquisition data analysis Require fractionation to dig deeper into proteome limited by dynamic range Best Quantitative Proteomic Experiments Defined question (i.e. hypothesis driven) Defined system Independently measurable phenotype Sample preparation Reproducible Scalable Compatible buffer system 5
6 Typical Quantitative Proteomic Experiment Fractionation? (enrich for target proteins) Sample Preparation (cell, tissue, fluid) (controls, experimentals) Labeling? (multiplex samples) Resolution on a common platform (proteins or peptides) (gel or column) Protein Quantification (protein or peptide level) Protein Identification (e.g. Tandem MS) Bioinformatics (statistics, ontology, pathways) Validation (e.g. western blot, MRM, etc) Sample Preparation (Most Important Step!) Reproducibility Protein Amount and Complexity Buffer Composition Compatibility Sample Preparation Must Be Reproducible and Standardized! Biological Replicates Initial Protocol Standardized Protocol Reduce Sources of Variability: Technical: good < bad hands few < many steps Biological: cells < tissue < body fluids yeast < nematode < human 6
7 Protein Amount and Complexity Detect Low Abundance Proteins by Fractionating MW kd MWS BSA Top 6 proteins removed Total Serum % SDS-PAGE, SimplyBlue stain 3. pi 11. 1D versus 2D versus Quantitative Proteomics Methods Non-Labeling Methods No additions to analysis Separate analysis of Each sample Biological Variability Technical Variability (Sample Prep and MS analysis) 7
8 Non-Labeling Densitometry -1D Gel Sample 2 1 Slice Gel slice Name of protein number Polymeric immunoglobulin 22 receptor Transferrin Vanin B-glycoprotein 21 Complement component 5 hgc-1 (human G-CSFstimulated 21 clone-1) IgG Fc-binding protein Mac-2-binding protein 18-1-antichymotrypsin t i 18 Albumin Comparing protein bands on same gel Quantify by densitometry Often more than one protein per band Kristiansen et al. Molec Cell Proteomics 3: , 24. No Labeling Densitometry - 2D Gels pi MW Bacterial Strain #1 Bacterial Strain #2 Compare spots from different gels Quantify by relative spot volume Gel reproducibility and spot matching critical Gel warping for matching spots can warp out modification Often more than one protein per spot No Labeling MS Methods Spectral Counting Each sample separate LCMS/MS experiment Quantify each protein from: number of peptides from protein number of spectra for each peptide Compares multiple LCMS/MS experiments Reproducibility of LCMS/MS system critical 8
9 No Labeling MS Methods Peak Intensity Each sample separate LCMS/MS experiment Quantify each protein from: intensity of 3 most intense peptides averaged from 3 technical replicates 3D Peak Intensity Map m/z Time Compares multiple LCMS/MS experiments Annotate Spots Reproducibility of LCMS/MS system critical Quantitative Proteomics Methods Non-Labeling Methods No additions to analysis Separate analysis of Each sample Biological and Technical Variability Labeling Methods Typically adds steps to analysis Simultaneous analysis of Many samples (Multiplexing) Biological Variability, Reduces Technical Variability Cuts instrument time (data collection) by 5-75% Difference Gel Electrophoresis (DIGE) Control Protein Extract Label with fluor 1 Disease Protein Extract Label with fluor 2 Mix labeled proteins from both samples Separate proteins on one 2-D gel Excitation wavelength 1 Image gel Excitation wavelength 2 Protein View differences comparison in a single gel! Unlu et al. Electrophoresis 18: ,
10 Novel Phosphorylation of Myofilament Protein Correlates with Myocardial Stunning Yuan et al. Proteomics 6: , 26 Three Fluorescent Cy Dyes Cy2, Cy3, Cy5!-amino group of lysine Matched for Charge and MW Cy2 Cy 5 Cy 3 Third Cy Dye used as an Internal Standard d All possible protein spots overlaid on every gel. Simplifies gel to gel matching. Each spot has it s own internal standard spot for normalizing across gels. Reduces experimental variations. Accounts for differences in sample load. DIGE Analysis Pooled Samples labeled with Cy2 (on all gels to compare one gel to another) Control Protein Extract Label with Cy3 Disease Protein Extract Label with Cy5 Mix samples Separate proteins on one 2-D gel Excitation wavelength 1 Excitation wavelength 2 Quantify intact proteins Cells, tissue, fluids One additional step Reduced technical variability 1
11 Relative MS Quantitative Methods Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Control Treated 12 C-Arg 13 C-Arg Metabolic labeling Only for cell culture Mix Cells No added steps No technical variability Standard Deviations 5% Isolate proteins and digest with trypsin Peptide ID and quantify by tandem MS Quantify mass pairs Ong et al. J Proteome Res 2: , O-labeling Protocol Control Protein Extract Trypsin Experimental Protein Extract Trypsin + 18 O-water C-terminal Enzymatic labeling Mix labeled peptides Cells, tissue, fluids No added steps Peptide ID and quantify by tandem MS No MS technical variability Standard deviations 1-2% Loss of label to water C-terminal (adds 4 Da) Yao et al. Anal. Chem 73: , 21 Yao et al. J Proteome Res 2: , 23 11
12 itraq Tags (Isobaric Tag for Relative and Absolute Quantitation) Isobaric Tag (Total mass = 35) Eight possible tags Reporter Balance PRG Reporter Mass Charged Balance Mass Peptide Reactive Group (binds to amines) 184 Ross et al. Mol. Cell. Proteomics 3: , 24 Pierce et al Mol. Cell Proteomics 7: ,27 Neutral 8-plex itraq Workflow Samples: S1 S2 S3 S4 S5 S6 S7 S8 Digestion: S1 S2 S3 S4 S5 S6 S7 S8 Label Peptides S1 113 S2 S3 S4 S5 S6 S7 S Chemical labeling Skip 12 Cells, tissue, fluids Phe (F) Mix Labeled Peptides Immonium ion Adds steps No MS technical variability Fractionate Peptide Mixture on SCX Column Analyze SCX Fractions by LC-MS/MS Intensity Eight Different Tags 7 Compare b1eight Samples in One Experiment y1 Quantifying Proteins using itraq y y b-ions itraq-a S Y L D mshc I R y-ions b2 y8 +2 b y b3 y5 y4 y b y8-itraq m/z 6 b6 5 4 Eight reporter ions quantify same peptide from eight samples in one spectrum one spectrum S S S S S2 2 S S2 S Skip m/z 12
13 b m/z, amu m/z, amu Changes in Protein Levels (biomarkers and pathways) Phospholipid Growth Factor (S1P) on Pulmonary Endothelial Permeability S1P Resistance Normalized R Resistance = Permeability Lipid Rafts? Proteins involved? Time (min) Guo Y et al (27) Mol Cell Proteomics 6: itraq Revealed Proteins More Abundant in S1P Stimulated Lipid Rafts MRP Peptide Fragmentation Western Blots Confirm itraq MRP_Human GDVTAEEAAGASPAK (3+) y 1 y 3 Control S1P Sample 1 Sample 2 Control S1P Control S1P A. Anti-MARCKS Intensity, counts y 2+ 3 y 2 2+ b B. Anti-p-MARCKS b 1 y 2+ 4 y 6 b b 3 b b 5 4 b b b 2 2+ b 9 y 4 y 5 y 7 C. Anti-MRP D. Anti-Caveolin-1 b b 9 MRP is up-regulated using both methods Guo Y et al (27) Mol Cell Proteomics 6:
14 MRP sirna Attenuates S1P Stimulated Endothelial Barrier Enhancement sistance Normalized Res S1P S1P+Scrambled MRP sirna S1P+MRP sirna MRP sirna or Scrambled MAP sirna Time (min) Guo Y et al (27) Mol Cell Proteomics 6: Can more than 8 samples be analyzed using 8-plex itraq? Experimental Design Yes! Pool of all samples: Standard in all itraq experiments Repeat labeling of at least 1 sample in all itraq experiments Completely randomize labeling Expected Result itraq 1 - Sample 1 itraq 1 - Pool = itraq 2 - Sample 1 itraq 2 - Pool itraq 1 - Sample 2 itraq 1 - Pool = itraq 2 - Sample 2 itraq 2 - Pool 14
15 Ratios for All Proteins in Sample 1 or Sample 2 Relative to Pool are the Same in itraq 1 and itraq 2 Ratios itraq # Sample 2 B47 y =.981x R 2 =.8611 Sample 1 A1249 y =.8426x R 2 = itraq #1 Ratios Micronutrient Deficiencies and Health of Undernourished Child and Maternal Health Problems Nutritional Deficiencies Infant/ Child Poor growth Impaired development Disability Infection Chronic disease Childhood Death Mother Obstetric morbidity Infection/sepsis Anemia Death Photo: K West Micronutrient Status Vitamin A, zinc, iron, iodine, folate, others Serum Protein Profiles? 5 samples 75 itraq experiments Gates Foundation Grant, PI: Keith West, JHSPH Changes in Protein Modifications (structure/function) 15
16 Using itraq to Quantify Site Specific Auto-Phosphorylation of the EGF Receptor Kinase Tyr MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLS 51 LQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIP 11 LENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRF 151 SNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCW 21 GAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLV 251 CRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYV 31 VTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLS 351 INATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKE 41 ITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGL 451 RSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCK 51 ATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFV 551 ENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVM 61 GENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGM 651 VGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPN 71 QALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREA 751 TSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLD 81 YVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQH 851 VKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSY 91 GVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKC 951 WMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYR Tyr 998 Qiu et al. 29 Biochemistry 48: Experimental Design Expressed EGFR Kinase Incubated +/- ATP and +/- kinase inhibitors Isolated EGFR Kinase Resolved EGFR Kinase by SDS-PAGE In gel digest, itraq label, SCX, LCMS/MS -ATP +ATP +EGFR +SRC Inhibitor Inhibitor EGFR Kinase kda -1 kda - 75 kda Qiu et al. 29 Biochemistry 48: EGFR Top Hit >35 peptide IDs with at least 95% confidence Same amount of EGRF in all gel bands (Ratios relative to No ATP sample labeled with 113) +ATP +EGFR +SRC kinase kinase Inhibitor Inhibitor Rank Score %Cov Name Species 115: : : Epidermal growth factor receptor precursor - Homo sapiens (Human) HUMAN Keratin, type II cytoskeletal 1 - Homo sapiens (Human) HUMAN Exportin-2 - Homo sapiens (Human) HUMAN Exportin-4 - Homo sapiens (Human) HUMAN Exportin-1 - Homo sapiens (Human) HUMAN Exportin-T - Homo sapiens (Human) HUMAN Keratin, type I cytoskeletal 1 - Homo sapiens (Human) HUMAN Uncharacterized protein C14orf139 - Homo sapiens (Human) HUMAN Qiu et al. 29 Biochemistry 48:
17 Phosphotyrosines: Phosphothreonines: Phosphoserines: Increase with ATP or Inhibitor to another kinase, but NOT with an Inhibitor to EGFR Kinase. No change No change +EGFR +Scr +ATP Kinase Kinase Alone Inhibitor Inhibitor Confidence Sequence Phosphate Modification 115: : : ELVEPLTPSGEAPNQALLR LLGAEEKEYHAEGGK LLGAEEKEYHAEGGKVPIK LLGAEEKEYHAEGGKVPIK MHLPSPTDSNFYR MHLPSPTDSNFYR MHLPSPTDSNFYR MHLPSPTDSNFYR 7 MHLPSPTDSNFYR Qiu et al. 29 Biochemistry 48: Kinetics (protein turnover, modification dynamics) Which peptide is a better substrate? Expermental Design: Two time courses (one for each peptide) Two itraq experiments (one for each time course) Substrate and products labeled with same itraq tag at each time point reagent Different itraq label for different time points Wade Gibson Time (hr) itraq Label Mix all itraq labeled substrates and proteins from all time points Run one MS analysis (LCMS/MS) for each time course 17
18 1 9 8 Peptide Peptide Substrate Substrate Product 1a Product 2a Product 1b Product 2b itraq Label Time (hr) Software for Identifying and Quantifying Proteins Label Free Sieve MSQuant msquant.alwaysdata.net Labeling ProteinPilot Mascot Scaffold Q+ Protein Discoverer 18
Protein Bioinformatics ( )
Protein Bioinformatics (260.655) Lecture 9: Quantitative Proteomics Tuesday, April 27, 2010 Robert N. Cole, Ph.D. Mass Spectrometry and Proteomics Facility Johns Hopkins School of Medicine 371 Broadway
More informationMass Spectrometry. Mass spectrometer MALDI-TOF ESI/MS/MS. Basic components. Ionization source Mass analyzer Detector
Mass Spectrometry MALDI-TOF ESI/MS/MS Mass spectrometer Basic components Ionization source Mass analyzer Detector 1 Principles of Mass Spectrometry Proteins are separated by mass to charge ratio (limit
More informationQuantification with Proteome Discoverer. Bernard Delanghe
Quantification with Proteome Discoverer Bernard Delanghe Overview: Which approach to use? Proteome Discoverer Quantification Method What When to use Metabolic labeling SILAC Cell culture systems Small
More informationLearning Objectives. Overview of topics to be discussed 10/25/2013 HIGH RESOLUTION MASS SPECTROMETRY (HRMS) IN DISCOVERY PROTEOMICS
HIGH RESOLUTION MASS SPECTROMETRY (HRMS) IN DISCOVERY PROTEOMICS A clinical proteomics perspective Michael L. Merchant, PhD School of Medicine, University of Louisville Louisville, KY Learning Objectives
More informationMultiplex Protein Quantitation using itraq Reagents in a Gel-Based Workflow
Multiplex Protein Quantitation using itraq Reagents in a Gel-Based Workflow Purpose Described herein is a workflow that combines the isobaric tagging reagents, itraq Reagents, with the separation power
More informationLecture 3. Tandem MS & Protein Sequencing
Lecture 3 Tandem MS & Protein Sequencing Nancy Allbritton, M.D., Ph.D. Department of Physiology & Biophysics 824-9137 (office) nlallbri@uci.edu Office- Rm D349 Medical Science D Bldg. Tandem MS Steps:
More information2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry
Dr. Sanjeeva Srivastava 1. Fundamental of Mass Spectrometry Role of MS and basic concepts 2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry 2 1 MS basic concepts Mass spectrometry - technique
More informationSUNY UPSTATE MEDICAL UNIVERSITY PROTEOMICS CORE
SUNY UPSTATE MEDICAL UNIVERSITY PROTEOMICS CORE Location: SUNY Upstate Medical University Weiskotten Hall Addition, Room 4303 750 East Adams Street Syracuse, NY 13210 Contact Information: David Kakhniashvili,
More informationLC/MS/MS SOLUTIONS FOR LIPIDOMICS. Biomarker and Omics Solutions FOR DISCOVERY AND TARGETED LIPIDOMICS
LC/MS/MS SOLUTIONS FOR LIPIDOMICS Biomarker and Omics Solutions FOR DISCOVERY AND TARGETED LIPIDOMICS Lipids play a key role in many biological processes, such as the formation of cell membranes and signaling
More informationImprove Protein Analysis with the New, Mass Spectrometry- Compatible ProteasMAX Surfactant
Improve Protein Analysis with the New, Mass Spectrometry- Compatible Surfactant ABSTRACT Incomplete solubilization and digestion and poor peptide recovery are frequent limitations in protein sample preparation
More informationSupporting Information. Lysine Propionylation to Boost Proteome Sequence. Coverage and Enable a Silent SILAC Strategy for
Supporting Information Lysine Propionylation to Boost Proteome Sequence Coverage and Enable a Silent SILAC Strategy for Relative Protein Quantification Christoph U. Schräder 1, Shaun Moore 1,2, Aaron A.
More informationProteomics/Peptidomics
Proteomics/Peptidomics System biology tools and preclinical models for translational research in endometriosis, ESHRE Campus workshop, 4-5 September 2009 E. Waelkens Proteomics: What? Proteins Proteomics
More informationLECTURE-15. itraq Clinical Applications HANDOUT. Isobaric Tagging for Relative and Absolute quantitation (itraq) is a quantitative MS
LECTURE-15 itraq Clinical Applications HANDOUT PREAMBLE Isobaric Tagging for Relative and Absolute quantitation (itraq) is a quantitative MS based method for quantifying proteins subject to various different
More informationPTM Discovery Method for Automated Identification and Sequencing of Phosphopeptides Using the Q TRAP LC/MS/MS System
Application Note LC/MS PTM Discovery Method for Automated Identification and Sequencing of Phosphopeptides Using the Q TRAP LC/MS/MS System Purpose This application note describes an automated workflow
More informationQuantification by Mass Spectrometry
Quantification by Mass Spectrometry PC219 Lecture 5 30 April, 2010 sguan@cgl.ucsf.edu 1 Applications of Quantitative Proteomics Qualitative protein identification is NOT sufficient to describe a biological
More informationQuantitation of Protein Phosphorylation Using Multiple Reaction Monitoring
Quantitation of Protein Phosphorylation Using Multiple Reaction Monitoring Application Note Authors Ning Tang, Christine A. Miller and Keith Waddell Agilent Technologies, Inc. Santa Clara, CA USA This
More informationProteins: Proteomics & Protein-Protein Interactions Part I
Proteins: Proteomics & Protein-Protein Interactions Part I Jesse Rinehart, PhD Department of Cellular & Molecular Physiology Systems Biology Institute DNA RNA PROTEIN DNA RNA PROTEIN Proteins: Proteomics
More informationAn Introduction to the Use of itraq Reagents for Amino Acid Analysis
An Introduction to the Use of itraq Reagents for Amino Acid Analysis Lisa Sapp Clinical Research and Forensic Toxicology Product Manager Mass Spectrometry Systems Amino Acid Analysis Using itraq Reagents
More informationOne Gene, Many Proteins. Applications of Mass Spectrometry to Proteomics. Why Proteomics? Raghothama Chaerkady, Ph.D.
Applications of Mass Spectrometry to Proteomics Raghothama Chaerkady, Ph.D. McKusick-Nathans Institute of Genetic Medicine and the Department of Biological Chemistry Why Proteomics? One Gene, Many Proteins
More informationComparison of mass spectrometers performances
Comparison of mass spectrometers performances Instrument Mass Mass Sensitivity resolution accuracy Quadrupole 1 x 10 3 0.1 Da* 0.5-1.0 pmol DE-MALDI 2 x 10 4 20 ppm 1-10 fmol peptide 1-5 pmol protein Ion
More informationNature Biotechnology: doi: /nbt Supplementary Figure 1
Supplementary Figure 1 The timeline of the NGAG method for extraction of N-linked glycans and glycosite-containing peptides. The timeline can be changed based on the number of samples. Supplementary Figure
More informationIntroduction to Proteomics 1.0
Introduction to Proteomics 1.0 CMSP Workshop Pratik Jagtap Managing Director, CMSP Objectives Why are we here? For participants: Learn basics of MS-based proteomics Learn what s necessary for success using
More information4-Plex itraq Based Quantitative Proteomic Analysis Using an Agilent Accurate -Mass Q-TOF
4-Plex itraq Based Quantitative Proteomic Analysis Using an Agilent Accurate -Mass Q-TOF Application Note Authors H. C. Harsha, G. S. S. Kumar, and A. Pandey Institute of Bioinformatics Bangalore India
More informationBiological Mass spectrometry in Protein Chemistry
Biological Mass spectrometry in Protein Chemistry Tuula Nyman Institute of Biotechnology tuula.nyman@helsinki.fi MASS SPECTROMETRY is an analytical technique that identifies the chemical composition of
More informationREDOX PROTEOMICS. Roman Zubarev.
REDOX PROTEOMICS Roman Zubarev Roman.Zubarev@ki.se Physiological Chemistry I, Department for Medical Biochemistry & Biophysics, Karolinska Institutet, Stockholm What is (RedOx) Proteomics? Proteomics -
More informationRDP Cores Highlights: the CF Analytics Core. Facundo M. Fernández School of Chemistry and Biochemistry Georgia Institute of Technology
CF@LANTA RDP Cores Highlights: the CF Analytics Core Facundo M. Fernández School of Chemistry and Biochemistry Georgia Institute of Technology CF@LANTA RDP Center The CF@LANTA RDP Center at Emory University
More informationShotgun Proteomics MS/MS. Protein Mixture. proteolysis. Peptide Mixture. Time. Abundance. Abundance. m/z. Abundance. m/z 2. Abundance.
Abundance Abundance Abundance Abundance Abundance Shotgun Proteomics Protein Mixture 1 2 3 MS/MS proteolysis m/z 2 3 Time µlc m/z MS 1 m/z Peptide Mixture m/z Block Diagram of a Mass Spectrometer Sample
More informationMass Spectrometry and Proteomics - Lecture 4 - Matthias Trost Newcastle University
Mass Spectrometry and Proteomics - Lecture 4 - Matthias Trost Newcastle University matthias.trost@ncl.ac.uk previously Peptide fragmentation Hybrid instruments 117 The Building Blocks of Life DNA RNA Proteins
More informationNew Instruments and Services
New Instruments and Services Liwen Zhang Mass Spectrometry and Proteomics Facility The Ohio State University Summer Workshop 2016 Thermo Orbitrap Fusion http://planetorbitrap.com/orbitrap fusion Thermo
More informationCharacterization of Disulfide Linkages in Proteins by 193 nm Ultraviolet Photodissociation (UVPD) Mass Spectrometry. Supporting Information
Characterization of Disulfide Linkages in Proteins by 193 nm Ultraviolet Photodissociation (UVPD) Mass Spectrometry M. Montana Quick, Christopher M. Crittenden, Jake A. Rosenberg, and Jennifer S. Brodbelt
More informationApplications of HPLC-MALDI-TOF MS/MS Phosphoproteomic Analysis in Oncological Clinical Diagnostics
Current Proteomics, 2011, 8, 153-167 153 Applications of HPLC-MALDI-TOF MS/MS Phosphoproteomic Analysis in Oncological Clinical Diagnostics Courtney L. Haddock*,1, Barry Holtz 1, Neil Senzer 1,2,3,4 and
More informationDevelopment of a Human Cell-Free Expression System to Generate Stable-Isotope-Labeled Protein Standards for Quantitative Mass Spectrometry
Development of a Human Cell-Free Expression System to Generate Stable-Isotope-Labeled Protein Standards for Quantitative Mass Spectrometry Ryan D. omgarden 1, Derek aerenwald 2, Eric Hommema 1, Scott Peterman
More information4.2 RESULTS AND DISCUSSION
phosphorylated proteins on treatment with Erlotinib.This chapter describes the finding of the SILAC experiment. 4.2 RESULTS AND DISCUSSION This is the first global report of its kind using dual strategies
More information4th Multidimensional Chromatography Workshop Toronto (January, 2013) Herman C. Lam, Ph.D. Calibration & Validation Group
4th Multidimensional Chromatography Workshop Toronto (January, 2013) Herman C. Lam, Ph.D. Calibration & Validation Group MDLC for Shotgun Proteomics Introduction General concepts Advantages Challenges
More informationSequence Identification And Spatial Distribution of Rat Brain Tryptic Peptides Using MALDI Mass Spectrometric Imaging
Sequence Identification And Spatial Distribution of Rat Brain Tryptic Peptides Using MALDI Mass Spectrometric Imaging AB SCIEX MALDI TOF/TOF* Systems Patrick Pribil AB SCIEX, Canada MALDI mass spectrometric
More informationAutomating Mass Spectrometry-Based Quantitative Glycomics using Tandem Mass Tag (TMT) Reagents with SimGlycan
PREMIER Biosoft Automating Mass Spectrometry-Based Quantitative Glycomics using Tandem Mass Tag (TMT) Reagents with SimGlycan Ne uaca2-3galb1-4glc NAcb1 6 Gal NAca -Thr 3 Ne uaca2-3galb1 Ningombam Sanjib
More informationLOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES. Gilles Frache Materials Characterization Day October 14 th 2016
LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES Gilles Frache Materials Characterization Day October 14 th 2016 1 MOLECULAR ANALYSES Which focus? LOCALIZATION of molecules by Mass Spectrometry
More informationProtein Reports CPTAC Common Data Analysis Pipeline (CDAP)
Protein Reports CPTAC Common Data Analysis Pipeline (CDAP) v. 05/03/2016 Summary The purpose of this document is to describe the protein reports generated as part of the CPTAC Common Data Analysis Pipeline
More informationGlycosylation analysis of blood plasma proteins
Glycosylation analysis of blood plasma proteins Thesis booklet Eszter Tóth Doctoral School of Pharmaceutical Sciences Semmelweis University Supervisor: Károly Vékey DSc Official reviewers: Borbála Dalmadiné
More informationAgilent Protein In-Gel Tryptic Digestion Kit
Agilent 5188-2749 Protein In-Gel Tryptic Digestion Kit Agilent Protein In-Gel Tryptic Digestion Kit Instructions Kit Contents The Protein In-Gel Tryptic Digestion Kit includes sufficient reagents for approximately
More informationDr. Erin E. Chambers Waters Corporation. Presented by Dr. Diego Rodriguez Cabaleiro Waters Europe Waters Corporation 1
Development of an SPE-LC/MS/MS Assay for the Simultaneous Quantification of Amyloid Beta Peptides in Cerebrospinal Fluid in Support of Alzheimer s Research Dr. Erin E. Chambers Waters Corporation Presented
More informationProtein Identification and Phosphorylation Site Determination by de novo sequencing using PepFrag TM MALDI-Sequencing kit
Application Note Tel: +82-54-223-2463 Fax : +82-54-223-2460 http://www.genomine.com venture ldg 306 Pohang techno park Pohang, kyungbuk, Korea(ROK) Protein Identification and Phosphorylation Site Determination
More informationElisabeth Huff Lonergan, PhD Steven M. Lonergan, PhD Mark J. Anderson, PhD
Elisabeth Huff Lonergan, PhD Steven M. Lonergan, PhD Mark J. Anderson, PhD The complexity of tenderness. Differences in tenderness due to postmortem aging is difficult to predict and manage Structure is
More informationDevelopment of a Bioanalytical Method for Quantification of Amyloid Beta Peptides in Cerebrospinal Fluid
Development of a Bioanalytical Method for Quantification of Amyloid Beta Peptides in Cerebrospinal Fluid Joanne ( 乔安妮 ) Mather Senior Scientist Waters Corporation Data courtesy of Erin Chambers and Mary
More informationMass Spectrometry and Proteomics Xudong Yao
Mass Spectrometry and Proteomics Xudong Yao Dept of Chemistry University of Connecticut Storrs, CT April 19, 2005 Proteomics and -omics Roles of mass spectrometry Comparative proteomics Gel or non-gel
More informationMS/MS to Targeted Proteomics (MRM)
MS/MS to Targeted Proteomics (MRM) How it worked on the Human Lens Proteome Jayson Falkner PhD jay@singleorganism.com Genes Show Limited Value in Predicting Diseases With only a few exceptions, what the
More informationMass Spectrometry and Proteomics. Professor Xudong Yao Bioanalytical Chemistry Spring 2007
Mass Spectrometry and Proteomics Professor Xudong Yao Bioanalytical Chemistry Spring 2007 Proteomics and -omics Roles of mass spectrometry Comparative proteomics Chemical proteomics Protein, Proteome and
More informationO O H. Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION EXPERIMENTAL. LC /MS conditions
Simplifying Qual/Quan Analysis in Discovery DMPK using UPLC and Xevo TQ MS Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION The determination of the drug metabolism
More informationMass Spectrometry Infrastructure
Mass Spectrometry Infrastructure Todd Williams, Ph.D. Director KU Mass Spectrometry and Analytical Proteomics Laboratory Mass Spectrometry Lab B025 Malott Hall Mission The Mass Spectrometry and analytical
More informationMass Spectrometry. - Introduction - Ion sources & sample introduction - Mass analyzers - Basics of biomolecule MS - Applications
- Introduction - Ion sources & sample introduction - Mass analyzers - Basics of biomolecule MS - Applications Adapted from Mass Spectrometry in Biotechnology Gary Siuzdak,, Academic Press 1996 1 Introduction
More informationTechnical Note # TN-31 Redefining MALDI-TOF/TOF Performance
Bruker Daltonics Technical Note # TN-31 Redefining MALDI-TOF/TOF Performance The new ultraflextreme exceeds all current expectations of MALDI-TOF/TOF technology: A proprietary khz smartbeam-ii TM MALDI
More informationIn-Gel Tryptic Digestion Kit
INSTRUCTIONS In-Gel Tryptic Digestion Kit 3747 N. Meridian Road P.O. Box 117 Rockford, IL 61105 89871 1468.2 Number Description 89871 In-Gel Tryptic Digestion Kit, sufficient reagents for approximately
More informationNature Methods: doi: /nmeth.3177
Supplementary Figure 1 Characterization of LysargiNase, trypsin and LysN missed cleavages. (a) Proportion of peptides identified in LysargiNase and trypsin digests of MDA-MB-231 cell lysates carrying 0,
More informationMASS SPECTROMETRY IN METABOLOMICS
For personal use only. Please do not reuse or reproduce without the author s permission MASS SPECTRMETRY IN METABLMICS Pavel Aronov Stanford Mass Spectrometry Users Meeting August 21, 2008 rigin of Metabolomics
More informationRelative Quantitation of Human Polymorphonuclear Leukocyte Cell Membrane GPEtn Lipids
Relative Quantitation of Human Polymorphonuclear Leukocyte Cell Membrane GPEtn Lipids Using the QTRAP System with mtraq Reagents Karin A. Zemski-Berry 1, John M. Hevko 2, and Robert C. Murphy 1 1 Department
More informationComparison of Relative Quantification of Monoclonal Antibody N-glycans Using Fluorescence and MS Detection
Comparison of Relative Quantification of Monoclonal ntibody N-glycans Using Fluorescence and MS Detection pplication Note iotherapeutics & iologics uthors scar Potter and Gregory Staples gilent Technologies,
More informationSupplementary Materials for
www.sciencesignaling.org/cgi/content/full/8/398/rs12/dc1 Supplementary Materials for Quantitative phosphoproteomics reveals new roles for the protein phosphatase PP6 in mitotic cells Scott F. Rusin, Kate
More informationDouble charge of 33kD peak A1 A2 B1 B2 M2+ M/z. ABRF Proteomics Research Group - Qualitative Proteomics Study Identifier Number 14146
Abstract The 2008 ABRF Proteomics Research Group Study offers participants the chance to participate in an anonymous study to identify qualitative differences between two protein preparations. We used
More information5 Identification of Binding Partners of the Annexin A2 / P11 Complex by Chemical Cross-Linking
5 Identification of Binding Partners of the Annexin A2 / P11 Complex by Chemical Cross-Linking In the quest of the omics sciences for holistic schemes, the identification of binding partners of proteins
More informationJose Castro-Perez, Henry Shion, Kate Yu, John Shockcor, Emma Marsden-Edwards, Jeff Goshawk Waters Corporation, Milford, MA, U.S. and Manchester, UK
HIGH-THRUGHPUT REACTIVE METABLITE SCREEIG FR DICLFEAC BY UPLC AD XEV TQ MS WITH SCAWAVE Jose Castro-Perez, Henry Shion, Kate Yu, John Shockcor, Emma Marsden-Edwards, Jeff Goshawk Waters Corporation, Milford,
More informationGlycerolipid Analysis. LC/MS/MS Analytical Services
Glycerolipid Analysis LC/MS/MS Analytical Services Molecular Characterization and Quantitation of Glycerophospholipids in Commercial Lecithins by High Performance Liquid Chromatography with Mass Spectrometric
More informationFor reprints and correspondence:
APPLICATION REPORT SEPTEMBER 30, 2015 New Proteomic Workflows Combine Albumin Depletion and On- Bead Digestion, for Quantitative Cancer Serum Haiyan Zheng 1 ; Caifeng Zhao 1 ; Meiqian Qian 1 ; Swapan Roy
More informationMASS SPECTROMETRY BASED METABOLOMICS. Pavel Aronov. ABRF2010 Metabolomics Research Group March 21, 2010
MASS SPECTROMETRY BASED METABOLOMICS Pavel Aronov ABRF2010 Metabolomics Research Group March 21, 2010 Types of Experiments in Metabolomics targeted non targeted Number of analyzed metabolites is limited
More informationSMART Digest Kit Facilitating perfect digestion
Questions Answers SMART Digest Kit Facilitating perfect digestion The modern biopharmaceutical and protein research laboratory is tasked with providing high quality analytical results, often in high-throughput,
More informationfor the Identification of Phosphorylated Peptides
Application of a Data Dependent Neutral-Loss Experiment on the Finnigan LTQ for the Identification of Phosphorylated Peptides Gargi Choudhary Diane Cho Thermo Electron, San Jose, CA Abstracted from posters
More informationProteomics of body liquids as a source for potential methods for medical diagnostics Prof. Dr. Evgeny Nikolaev
Proteomics of body liquids as a source for potential methods for medical diagnostics Prof. Dr. Evgeny Nikolaev Institute for Biochemical Physics, Rus. Acad. Sci., Moscow, Russia. Institute for Energy Problems
More informationQuantitative Proteomics. Quantitative Proteomics
Quantitative Proteomics Liwen Zhang Mass Spectrometry and Proteomics Facility The Ohio State University Summer Workshop 216 Quantitative Proteomics Quantitation in proteomics has become a popular area
More informationMass spectra of peptides and proteins - and LC analysis of proteomes Stephen Barnes, PhD
Mass spectra of peptides and proteins - and LC analysis of proteomes Stephen Barnes, PhD 4-7117 sbarnes@uab.edu Overview A mass spectrum Electrospray MS Analysis of intact proteins Molecular weight calculations
More informationApplication Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform
Application Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform Abstract Targeted proteomics for biomarker verification/validation
More informationMetabolite identification in metabolomics: Metlin Database and interpretation of MSMS spectra
Metabolite identification in metabolomics: Metlin Database and interpretation of MSMS spectra Jeevan K. Prasain, PhD Department of Pharmacology and Toxicology, UAB jprasain@uab.edu Outline Introduction
More informationEnhancing Sequence Coverage in Proteomics Studies by Using a Combination of Proteolytic Enzymes
Enhancing Sequence Coverage in Proteomics Studies by Using a Combination of Proteolytic Enzymes Dominic Baeumlisberger 2, Christopher Kurz 3, Tabiwang N. Arrey, Marion Rohmer 2, Carola Schiller 3, Thomas
More informationNew Instruments and Services
New Instruments and Services http://planetorbitrap.com/orbitrap fusion Combining the best of quadrupole, Orbitrap, and ion trap mass analysis in a revolutionary Tribrid architecture, the Orbitrap Fusion
More informationMetabolite identification in metabolomics: Database and interpretation of MSMS spectra
Metabolite identification in metabolomics: Database and interpretation of MSMS spectra Jeevan K. Prasain, PhD Department of Pharmacology and Toxicology, UAB jprasain@uab.edu utline Introduction Putative
More informationLC-MS/MS for the quantification of Peptide biomarker and mixture of closely related Protein in formulation
EUROPEAN BIOANALYSIS FORUM Barcelona, November 14-16, 2012 LC-MS/MS for the quantification of Peptide biomarker and mixture of closely related Protein in formulation Luc-Alain SAVOY CONTENT Part I: SGS
More informationMass Spectrometry at the Laboratory of Food Chemistry. Edwin Bakx Laboratory of Food Chemistry Wageningen University
Mass Spectrometry at the Wageningen University Mass Spectrometry at the 3 UPLC/CE - ESI - Ion trap MS systems UPLC Thermo Acella with a Velos or VelosPro CE Beckman PA800 with a Thermo VelosPro 1 UPLC-
More informationFigure S6. A-J) Annotated UVPD mass spectra for top ten peptides found among the peptides identified by Byonic but not SEQUEST + Percolator.
Extending Proteome Coverage by Combining MS/MS Methods and a Modified Bioinformatics Platform adapted for Database Searching of Positive and Negative Polarity 193 nm Ultraviolet Photodissociation Mass
More informationNIH Public Access Author Manuscript J Proteome Res. Author manuscript; available in PMC 2014 July 05.
NIH Public Access Author Manuscript Published in final edited form as: J Proteome Res. 2013 July 5; 12(7): 3071 3086. doi:10.1021/pr3011588. Evaluation and Optimization of Mass Spectrometric Settings during
More informationMass-Spectrometric Analysis of Lipids (Lipidomics)
Mass-Spectrometric Analysis of Lipids (Lipidomics) 1. Identification 2. Quantification 3. Metabolism Why to do lipidomics? Biology: Functions of different lipids? Medicine: Diagnostics and Therapy Industry:
More informationA computational framework for discovery of glycoproteomic biomarkers
A computational framework for discovery of glycoproteomic biomarkers Haixu Tang, Anoop Mayampurath, Chuan-Yih Yu Indiana University, Bloomington Yehia Mechref, Erwang Song Texas Tech University 1 Goal:
More informationMSSimulator. Simulation of Mass Spectrometry Data. Chris Bielow, Stephan Aiche, Sandro Andreotti, Knut Reinert FU Berlin, Germany
Chris Bielow Algorithmic Bioinformatics, Institute for Computer Science MSSimulator Chris Bielow, Stephan Aiche, Sandro Andreotti, Knut Reinert FU Berlin, Germany Simulation of Mass Spectrometry Data Motivation
More informationUse of Derivatization for LC- MS/MS Analysis in the Clinical Lab
Use of Derivatization for LC- MS/MS Analysis in the Clinical Lab Asian Pacific Conference on Chromatography & Mass Spectrometry 2010 15 January 2010 Alan L. Rockwood and Mark M. Kushnir ARUP Laboratories,
More informationMALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH. December 19,
MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH December 19, 2014 1 The principle of MALDI imaging Spatially resolved mass spectra are recorded Each mass signal represents
More informationMore structural information with MS n
PRODUCT SPECIFICATIONS The LTQ XL linear ion trap mass spectrometer More structural information with MS n The LTQ XL linear ion trap mass spectrometer delivers more structural information faster and with
More informationSIEVE 2.1 Proteomics Example
SIEVE 2.1 Proteomics Example Software Overview What is SIEVE? SIEVE is Thermo Scientific s differential software solution. SIEVE will continue to enhance our current product for label-free differential
More informationWhat Impact will Proteomics, Including CSF Analysis, have on the Near-term Development of Biomarkers for Nervous System Diseases?
What Impact will Proteomics, Including CSF Analysis, have on the Near-term Development of Biomarkers for Nervous System Diseases? Daumier 1 Howard Schulman (howard.schulman@menlo.ppdi.com) Biomarker Discovery,
More informationThe use of mass spectrometry in lipidomics. Outlines
The use of mass spectrometry in lipidomics Jeevan Prasain jprasain@uab.edu 6-2612 utlines Brief introduction to lipidomics Analytical methodology: MS/MS structure elucidation of phospholipids Phospholipid
More informationDiscovery Metabolomics - Quantitative Profiling of the Metabolome using TripleTOF Technology
ANSWERS FOR SCIENCE. KNOWLEDGE FOR LIFE. Discovery Metabolomics - Quantitative Profiling of the Metabolome using TripleTOF Technology Baljit Ubhi Ph.D ASMS Baltimore, June 2014 What is Metabolomics? Also
More informationNew Developments in LC-IMS-MS Proteomic Measurements and Informatic Analyses
New Developments in LC-IMS-MS Proteomic Measurements and Informatic Analyses Erin Shammel Baker Kristin E. Burnum-Johnson, Xing Zhang, Cameron P. Casey, Yehia M. Ibrahim, Matthew E. Monroe, Tao Liu, Brendan
More informationSection 1 Proteins and Proteomics
Section 1 Proteins and Proteomics Learning Objectives At the end of this assignment, you should be able to: 1. Draw the chemical structure of an amino acid and small peptide. 2. Describe the difference
More informationMALDI-TOF. Introduction. Schematic and Theory of MALDI
MALDI-TOF Proteins and peptides have been characterized by high pressure liquid chromatography (HPLC) or SDS PAGE by generating peptide maps. These peptide maps have been used as fingerprints of protein
More informationTECHNICAL BULLETIN. R 2 GlcNAcβ1 4GlcNAcβ1 Asn
GlycoProfile II Enzymatic In-Solution N-Deglycosylation Kit Product Code PP0201 Storage Temperature 2 8 C TECHNICAL BULLETIN Product Description Glycosylation is one of the most common posttranslational
More informationDigitizing the Proteomes From Big Tissue Biobanks
Digitizing the Proteomes From Big Tissue Biobanks Analyzing 24 Proteomes Per Day by Microflow SWATH Acquisition and Spectronaut Pulsar Analysis Jan Muntel 1, Nick Morrice 2, Roland M. Bruderer 1, Lukas
More informationTypes of Modifications
Modifications 1 Types of Modifications Post-translational Phosphorylation, acetylation Artefacts Oxidation, acetylation Derivatisation Alkylation of cysteine, ICAT, SILAC Sequence variants Errors, SNP
More informationAdvantages of Ion Mobility Q-TOF for Characterization of Diverse Biological Molecules
Advantages of Ion Mobility Q-TOF for Characterization of Diverse Biological Molecules Add a New Dimension to your Research Capability with Agilent s New Drift Ion Mobility Q-TOF System Overview: 6560 IM
More informationApplying a Novel Glycan Tagging Reagent, RapiFluor-MS, and an Integrated UPLC-FLR/QTof MS System for Low Abundant N-Glycan Analysis
Applying a Novel Glycan Tagging Reagent, RapiFluor-MS, and an Integrated UPLC-FLR/QTof MS System for Low Abundant N-Glycan Analysis Ying Qing Yu Waters Corporation, Milford, MA, USA APPLICATION BENEFITS
More informationQuantification of PtdInsP 3 molecular species in cells and tissues by mass spectrometry
Nature Methods Quantification of PtdInsP 3 molecular species in cells and tissues by mass spectrometry Jonathan Clark, Karen E Anderson, Veronique Juvin, Trevor S Smith, Fredrik Karpe, Michael J Wakelam,
More informationSUPPORTING INFORMATION. Lysine Carbonylation is a Previously Unrecognized Contributor. to Peroxidase Activation of Cytochrome c by Chloramine-T
Electronic Supplementary Material (ESI) for Chemical Science. This journal is The Royal Society of Chemistry 2019 SUPPORTING INFORMATION Lysine Carbonylation is a Previously Unrecognized Contributor to
More informationMethods in Mass Spectrometry. Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University
Methods in Mass Spectrometry Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University Sample Engineering Chemistry Biology Life Science Medicine Industry IDF / Police Sample
More informationLatest Innovations in LC/MS/MS from Waters for Metabolism and Bioanalytical Applications
Latest Innovations in LC/MS/MS from Waters for Metabolism and Bioanalytical Applications Ignatius J. Kass Senior Field Marketing Manager Pharmaceutical MS Challenges in Pharmaceutical Sample Analysis Quantitative
More informationTrypsin Mass Spectrometry Grade
058PR-03 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Trypsin Mass Spectrometry Grade A Chemically Modified, TPCK treated, Affinity Purified
More information