Project-wise Reports of Sectorial Monitoring Committee
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1 Project-wise Reports of Sectorial Monitoring Committee 1. Project Title: Lipidomic Center (LIPIC) 2. Project Details: Nodal Lab Participating Labs Nodal Officer Taskforce Chairman Total Man Days Approved Cost Fund received Fund utilized CSIR-CFTRI NONE Dr Malathi Srinivasan Prof Ram Rajasekharan days INR Cr INR Cr - INR Cr 3. Periodic Key Recommendations of Task Force/RC/Monitoring Committee - Kindly refer to the Minutes of the Task force and RC meetings. S.No 1. Key Action taken on Key Proposed Recommendations of Recommendations Deliverables Task Force/RC/ Monitoring Committee Data mining of chia transcriptome Under way More lipid related genes to be characterized Revised Deliverable (approved by Task Force) Chia seed and oil products Completed High yielding variety High EPA yielding Portulaca Several Indian and exotic accessions were screened Use of chia seed mucilage as biopolymer Thin biofilms were created A biopolymer to increase shelf life of fruits Transgenics to see if delta 6 desaturase can convert ALA to stearidonic acid (SDA) Plasmid was procured from Rothamstead and tried in yeast system Chia plant that can synthesize SDA New SDA synthesizing plants were taken up for further studies 2. To make a comparative EPA, DHA levels in Leaf lipid with high study of indigenous and the neutral lipids of EPA, DHA exotic accessions of the leaves were Focus is currently on the polar lipids 1
2 Purslane for yield and quality (fatty acid profile) parameters To explore newer lipid yielding plants To biochemically characterize the ShMAGAT and ShOLEOSIN, and identify more lipid related genes/ enzymes assessed Lettuce, Bugglosoides etc in the pipeline Completed A moonlighting enzyme with dual functionality has been characterized Adaptation and acclimatization of Echium for commercial cultivation Completed Echium oil rich in SDA, no clearance for edible purpose Moved to edible SDA producing plants 4. Project Achievements: S. No. Deliverables Deliverables Achieved If not achieved, reasons thereof promised at start (as provided in the SFC document) 1. WP1- a fully Achieved only in 2016, as the NOT APPLICABLE established Lipidomics earlier proposal to have the Center at CSIR- center in CSIR-CIMAP RC CIMAP Research Bangalore was disallowed Center, Bangalore - and the facility had to be Aug 2013 created in CSIR-CFTRI, Mysore. The current LIPIDOMICS CENTER is at CSIR-CFTRI, Mysore 2. WP2 - Complete This was completed. A STED NOT APPLICABLE Instrumentation Leica microscope was facilities and procured and installed at standardized SOPs- CIMAP/CFTRI RC Bangalore Aug 2014 in 2013; an Agilent GC/MS and Sciex HRMS were installed at CFTRI in Staff and students were imparted training and the instruments were functional. 3. WP3 - Research Mostly done and still in NOT APPLICABLE project outcomes using progress. high end lipidomic Two new varieties of Chia approaches - March released and > farmer beneficiaries 15 publications in SCI indexed journals (list provided below) 2
3 Completion of the transcriptome of chia, portulaca, ocimum, lettuce and Buglossoides Several new theories on transcriptional regulation have been proposed for the lipid metabolism in yeast Extensive chemical characterization completed for the oils extracted from chia, basil and lettuce 5. Outcomes and outputs from the projects: a. Outcomes 1. Lead(s) identified: Number and names with TRL- Agrotechnology for oil seed lettuce and Buglossoides- TRL-5 Stearidonic acid rich oil - TRL -4 Omega 3 fatty acid rich oils for blending- TRL- 5 Capsules of ALA as Nutraceuticals - TRL- 4 Transcription factors in yeast and plant systems that regulate lipid metabolism - Not applicable 2. Technology/Product developed: Number and names with TRL level of Development 3. Technology/Product transferred: Number and names with name of licensee(s) (i) This program resulted in the formulation of agrotechnology for growing the oil seed crop Chia in India, which is originally from Mexico. It was decided to provide this technology for free to the farmers of the country along with 1 OOg of seeds to ensure they grow and get benefited from it. It was purely a societal intervention, resulting in more than 2000 farmer beneficiaries. (ii) Technologies towards making healthy icecreams and chocobars were developed. Omega- 3 fatty acid in the form of oil was added to make nutritious and healthy icecreams called Nutriice, while crispy seeds were incorporated into chocobars to make chocolates healthier. Both technologies have already been successfully transferred. 4. Facility created: Number and names with uniqueness & utility A state-of-the-art Lipid Research facility that can house about 50 scientists (Faculty & their research scholars and project assistants) with a well-equipped cell culture facility, common instrumentation facilities and inoculation rooms was created. A walk-in cold room and a walk-in plant growth chamber equipped with a high end Biolistic Gene gun were also created. Infrastructure like an SP8 confocal STED Leica microscope with a white light laser and live cell imaging, a first of its kind in the country, was procured under this project and is being extensively used; The High Resolution Mass spectrometer from ABSciex (now, Sciex) is the 3
4 one recommended especially for lipid analysis and the same was procured under this project. In India, these equipments are available only at CSIR-CFTRI to enable quality and specific lipid research. Similar such set ups are found at the Kansas Lipid research Center (KLRC) and at the National University of Singapore (NUS). 5. Sponsored projects obtained (with details)- NOT APPLICABLE 6. External Cash Flow (ECF) generated- NOT APPLICABLE 7. Societal impact created b. Outputs: As a societal endeavour, we had released the seed varieties to the farmers for free in October 2014 and provided them with the agro-technology to grow the same. More than 2000 farmers have benefited by this and have seen a steady rise in their income and economic status. 1. Periodic Reports Submitted- Annual reports to CSIR Headquarters and to CSIR-CFTRI 2. Publications i. Totalnumber - 12 ii. Cumulative IF (as per 2015 figures) iii. Average IF iv. Average IF/scientist- RR 3.112; MS 3.018; RVS Patents (or other forms of IP generated such as Copyrights, Designs etc.)- NONE 4. Human Resource generated i. PhD fellows (give PhDs completed & pursuing separately) - 5 completed; 7 ongoing ii. Project fellows -10 PAs hired through the project period, 5 currently on roll iii. Skills imparted - Including skill set developed for technical services/ consultancy/ training purposes Students have been trained to operate high end equipments like the Confocal Live cell imager, GCJMS and HRMS 5. External collaborations established s. No 1 Lead/T echnology/pr Salient TRL of the IP Status of Status of oduct (listed above Lead/ Lead/Technology/Pr DPR/Commercial Features of the in outcomes) Technology oduct Technical Report of Lead/Tech nolo /Product Lead/ Technology/ gy/ Product Product Agrotechnology for These are new 5 oil seed lettuce and crops that Buglossoides have not been systematically cultivated so far, and we have the technology to grow them 4
5 2 Stearidonic acid SDA is a better 4 rich oil precursor for EPA DHA conversion compared to ALA 3 Omega 3 fatty acid Hitherto 5 rich oils for cooking oils blending have never had omega 3 fatty acid. This will be the first time to introduce healthy omega 3 fatty acid into cooking oils 4 Capsules of ALA as This will be the 4 Nutraceuticals first plant based source of ALA in capsules 6. Sectoral Monitoring Committee Recommendations on Lead/Technology/Product (listed above): For each Lead/Technology/Product provide details separately: Criteria SMC Recommendations Name of Lead/Technology /Product The facility for lipid R & Dis created. Is the Lead/ Technology/Product worth taking up further? What is the further R&D efforts that need to be put by CSIRICSIR laboratories? Yes The following activities will be further continued: The development of transcriptome and lipidome data of other crops, viz., ocimum seed, lettuce seed, portulaca leaf and buglossoides seed to characterize genes in the plant lipid biosynthetic pathway The yeast data pertaining to several physiological conditions like ageing, micronutrient deficiency, mitochondrial dysfunction etc. with relation to their lipid profiles can be extrapolated to human conditions as well Global benchmarking of the The products that are being developed are for Indian society and Lead/Technology/Product made affordable. specifically wrt specifications and cost Publications in Journals of International repute 5
6 Is the Lead/Technology/Product No worthy of commissioning a DPR at this stage? What are the likely resources and time duration required for taking forward the identified Lead/ Technology/Product to the desired TRL? Potential stake holders who may be appropriate to partner technically as well as financially Suggestion for plausible road map towards further development of lead/technology/product for achieving desired TRL GO/NO GO further development Other suggestions, if any, related to benefits/usage/ commercialization Financial support would be required for 3+2 years. Farmers, Industries and Indian society at large. Details would be mentioned once project proposal in detail would be presented Go None 7. Identified "lessons to be learnt", especially from shortcomings/failures. Greater programmatic alignment would further strengthen the likely outcomes and impacts. 8. Comments on financial progress on the project fund availability and utilization of fund in the project: The financial progress is satisfactory and the investment made is justified. 9. Sectoral Monitoring Committee Recommendations on Facility creation or other outcomes: Experiences gained, materials generated and lessons learnt are expected to have far reaching impacts in years to come. 10. Grading of Project Execution: Outstanding/Excellent/Very Good/Good/Satisfactory/Un-satisfactory Excellent 11. Additional comments, if any: None Dr. udeep Kumar Dr. B. Sasikeran ~~ Dr. R.S. Sangwan Prof. Akhilesh Tyagi Dr. Suresh Ramamurthi Dr.T.~.. 6
7 Dr. Prosenjit Bose Dr. Sudhir Achar Director, CSIR-CCMB (TF Chairman - Member) Direc r, CSJR-CFTRI (TF Chairman - Member) Direc~-C I MAP (TF Chairman- Member) Directo, CSI T (TF Chairman - Member) \L L-- ~~;.It- Director, CSIR-IICT Director, CSIR-NBRI Director, CSIR-NIIST (TF Chairman - Member) (TF Chairman - Member) (TF Chairman - Member) Director, CSIR- NCL s~ (TF Chairman -Member) Dr. Arjula Ramachandra Reddy ~ ~ Dr. Mangala Rai (Chairman) 7
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