CLINICAL RESEARCH APPLICATIONS GUIDE

Transcription

1 CLINICAL RESEARC APPLICATINS GUIDE

2 Your job has never demanded more from you. Doctors need accurate results yesterday. You need to minimize costs while increasing the number of samples run. New tests require months and months of design and validation. is committed to providing complete solutions that meet your needs. From sample preparation, to chromatographic separation to detection, we are constantly working to achieve greater sensitivity, shorter run times and improved separations that that your lab can rely on. Since 98, has grown to become a leading provider of advanced technology solutions for separation science techniques in the areas of igh-performance Liquid Chromatography (PLC/UPLC Gas Chromatography (GC More throughput Greater sensitivity Reduced costs Less downtime LC/MS and LC/MS/MS Applications Aldosterone... 4 Catecholamines... 6 Digitoxin and Digoxin... 7 Estrogens... 8 Gabapentin... istamine... Metanephrine and Normetanephrine... 4 Nicotine and its Metabolites... 6 Methylmalonic Acid (MMA... 8 Pain Panel Progesterone... Steroids Panel... 4 Testosterone Vitamin D and D Vitamin D and -epi---vitamin D... Vitamin B6 (Water Soluble... Vitamins (Fat Soluble... 4 Vitamin A... Vitamin D... Vitamin E... Vitamin K... Bath Salts... EtG and EtS... 6 GC/MS Application Very Long Chain Fatty Acids... 7 Solutions PLC Columns... 8 Solid Phase Extraction (SPE Products... 4 PhenoLogix SM Method Development Method Improvement Validation Services This icon indicates an AB SCIEX imethod Application featuring products is available for this analysis. imethods are the easiest way to get started on an LC/MS/MS method if you are using Cliquid Software. Contact or AB SCIEX for more details. For Research Use nly. Not for use in diagnostic procedures. Inc. All rights reserved. Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: -- Puerto Rico Tel: Puerto Rico Tel:

3 Aldosterone verproduction of Aldosterone in the body can lead to primary hyperaldostronism and hypertension. Clinical monitoring of Aldosterone in serum is one of the methods used to understand the cause of hypertension as well as to apply an effective and proper treatment. Excellent sensitivity down to ng/dl Summary: Part No.: Strata -X-A 6 mg/ ml cartridges were utilized to extract Aldosterone from serum samples. 8B-S-UBJ Sample Pretreatment. Into individually labeled test tube combine. ml serum sample (or calibrator or QC sample, ml mm ammonium bicarbonate (p and. ml working internal standard solution.. Proceed to SPE procedure SPE Procedure. Condition: ml % Methanol. Equilibrate: ml mm Ammonium bicarbonate. Load: Pretreated sample 4. Wash : ml mm Ammonium bicarbonate. Wash : ml % Methanol in Water 6. Dry: min at high vacuum 7. Elute: x ml Ethyl Acetate/Isopropanol/N 4 (7:: 8. Evaporate: to - C under a gentle nitrogen stream 9. Reconstitute: µl of Methanol/Water (:7 containing ~ ppm Estriol Aldosterone Also available in 96-well format Aldosterone LC/MS/MS Method Analyte Q Q Aldo Aldo 9.. IS (Aldo-D Chromatogram of an extracted serum standard..e4.e4.e4 e min A typical calibration range from - ng/dl. Corr Coeff = Column: Kinetex.6 µm C8 Dimensions: x. mm Part No.: B-4496-Y Guard: SecurityGuard ULTRA guard cartridge system Part Nos.: AJ-9 (holder AJ-877 (C8 cartridges Mobile phase A: DI Water B: Acetonitrile/Methanol (: Flow Rate:. ml/min Column Temp: Ambient Injection Volume: µl Gradient : Time (min 4. 7 Sample:. Aldosterone. Aldosterone-D7 9 9 App ID 9 Tip Transferable to a mm ID column at.4 ml/min Questions or Requests? Contact ClinicalResearch@phenomenex.com for questions on applications or to request additional applications. Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: -- 4 Puerto Rico Tel: Puerto Rico Tel:

4 Catecholamines Catecholamines are hormones produced by the adrenal glands that are released into the blood during times of stress. Their measurements can aid in the diagnosis of tumors of the adrenal glands such as pheochromocytoma and the cause hypertension. Fast run time and excellent separation with Kinetex core-shell PLC/ UPLC columns Extend column lifetime, minimizing down time and costs, with SecurityGuard ULTRA guard cartridge system PLC Method Column: Kinetex.6 µm C8 Dimensions: x 4.6 mm Part No.: F-446-E Guard: SecurityGuard ULTRA guard cartridge system Part No.: AJ-9 (holder; AJ-8768 (C8 cartridges, /pk Mobile Phase: A: mm Ammonium formate buffer, p = / Methanol (97: Elution Type: Isocratic Flow Rate:. ml/min Detection: 8 nm Temperature: Ambient Sample:. Norepinephrine. Epinephrine. L-DPA 4. Dopamine. Serotonin 874 mau (R Norepinephrine Epinephrine Dopamine N N N N Digitoxin and Digoxin in Plasma Digitoxin and Digoxin are the active compounds in some drugs used to treat cardiac failure. Both compounds have a narrow therapeutic index, making therapeutic monitoring essential to effective patient treatment. Excellent sample clean up with Strata -X SPE Fast run time and excellent separation with Kinetex core-shell PLC/ UPLC columns Summary: Strata-X mg/ ml cartridges were used to extract the analytes from plasma. Part No.: 8B-S-TBJ SPE Procedure. Condition: ml ml/min. Equilibrate: ml mm Ammonium ml/min. Load: sample 4. Wash: ml mm Ammonium acetate/ mm Ammonium acetate ml/min. Dry: minutes at full vacuum 6. Elute: ml ml/min LC/MS/MS Analysis Digitoxin Digoxin Analyte Q Q Digoxin leandrin (IS Digitoxin Also available in 96-well format App ID 874 N Serotonin Column: Kinetex.6 µm C8 Å Dimensions: x. mm Part No.: B-4497-AN Mobile Phase: A: mm Ammonium acetate B: mm Ammonium acetate in Methanol Gradient: Time (min.. Flow Rate: 4 μl/min Temperature: C Detection: C Sample:. Digoxin. leandrin (IS. Digitoxin min App ID min Questions or Requests? Contact ClinicalResearch@phenomenex.com for questions on applications or to request additional applications. Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: Puerto Rico Tel: Puerto Rico Tel:

5 Estrogens Estrogens are a group of steroids that are the primary female sex hormones. The three major estrogens in women are estrone (E, estradiol (E, and estriol (E. Estrogen assays may be used to diagnose a wide variety of disorders and conditions. Estrone Estrogens Method. Example chromatogram for pg/ml of Estrone and Estradiol generated on an API system in a six-minute run. 6-minute run time Method can be used for quantitation with a linear range from to pg/ml Long column lifetime under high p conditions with Gemini TWIN technology PLC columns Estradiol LC/MS/MS Analysis Method : Estrone and Estradiol Column: Gemini µm C6-Phenyl Dimensions: x. mm Part No.: B-444-B Mobile Phase: A: Water +. % of. N Ammonium hydroxide B: Methanol +. % of. N Ammonium hydroxide Flow Rate:. ml/min Gradient: Time (min Detector: API 8 9 Analyte Q Q Estrone Estradiol d4-estrone d-estradiol Method : Estriol Column: Gemini µm C6-Phenyl Dimensions: x. mm Part No.: B-444-B Mobile Phase: A: Water +. % of. N Ammonium hydroxide B: Methanol +. % of. N Ammonium hydroxide Flow Rate:. ml/min Gradient: Time (min Analyte Q Q Estriol D-Estriol Estriol imethod Available Contact AB SCIEX or for details AB SCIEX imethod Test for Estrogens Version. for Cliquid Software Method. Example chromatogram for pg/ml of Estriol generated on an API system in a six minute run Estriol S/N = min.8 Representative calibration curve for Estrone. The assay was linear across the concentration range of - pg/ml Representative calibration curve for Estriol. The assay was linear across the concentration range of - pg/ml min D -Estriol App ID 6 App ID 64 Representative calibration curve for Estradiol. The assay was linear across the concentration range of - pg/ml Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: Puerto Rico Tel: Puerto Rico Tel: Questions or Requests? Contact ClinicalResearch@phenomenex.com for questions on applications or to request additional applications.

6 Gabapentin Gabapentin is a pharmaceutical drug which has a wide range of uses including the treatment of neuropathic pain, seizures, menopause and treatment of methamphetamine, cocaine, and alcohol addiction. Fast run time with Kinetex core-shell technology PLC/UPLC columns Excellent linearity from. µg/ml- µg/ml. Transfer: µl of plasma to a. ml centrifuge tube. Add: µl of IS/ %TCA solution to the centrifuge tube, mix briefly. 4, rpm for min 4. Transfer: µl of the supernatant to an autosampler vial and QS to ml with water. At this point the sample is ready for analysis. Gabapentin N Gabapentin LC/MS/MS Analysis Analyte Q Q Gabapentin Gabapentin D4-Gabapentin Gabapentin extracted from plasma using protein precipitation with trichloroacetic acid at a concentration of µg/ml. 649.e.e.8e.6e.4e.e.e 8.e4 Column: Kinetex.6 µm C8 Å Dimensions: x. mm Part No.: B-446-AN Mobile Phase: A: mm Ammonium formate in water B: Acetonitrile Gradient: Time (min min. min. min. min 9 Flow Rate:.4 ml/min Injection Volume:. µl Temperature: C (column Detector: API Sample:. D4 Gabapentin. Gabapentin 6.e4 4.e4.e4 App ID min Gabapentin calibration curve from. µg/ml- µg/ml extracted from plasma using protein precipitation with trichloroacetic acid Questions or Requests? Contact ClinicalResearch@phenomenex.com for questions on applications or to request additional applications.. Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: -- Puerto Rico Tel: Puerto Rico Tel:

7 istamine istamine istamine is involved in immune responses and mediating many important physiological functions such as the regulation of the immune system, nervous system and the secretion of gastric acids. istamine is formed by the decarboxylation of L-istidine, an essential amino acid. Good retention and separation using Luna ILIC PLC columns Excellent sample clean up with Strata -X-CW SPE Sample prep istamine was extracted from plasma using Strata-X-CW, mg/ ml cartridges Part No.: 8B-S-TBJ Sample Pretreatment. Add. ml IS solution containing 4 ng/ml histmine-d4 and I-methylhistamine-d prepared in DI water to µl sample, blank, or plasma. Proceed to SPE procedure SPE Procedure. Condition: ml % Methanol. Equilibrate: ml DI Water L-istidine istadine decarboxylase istamine LC/MS/MS Analysis Analyte Q Q istamine. 9. istamine M-istamine M-istamine N-M-istamine N-M-istamine istamine-d Methyl-ist-D e4.6e4.e4.8e4.4e4.e4.6e4.e min.4e4.e4.e min Column: Luna ILIC µm Dimensions: x. mm Part No.: D-4449-B Mobile Phase: A: Acetonitrile/DI Water/ mm Ammonium formate, p. (:4: B: Acetonitrile: mm Ammonium formate, p. (9: Flow Rate:.6 ml/min Column Temp: Ambient Injection Volume.: µl Gradient: Time (min Sample:. -M-istamine. N-M-istamine. istamine. Load: pretreated sample 4. Wash : ml DI Water. Wash : ml Methanol 6. Dry: min at high vacuum level, ~- in g 7. Elute: x µl portions of initial mobile phase composition containing B/A (87:, fortified to contain % Formic acid 8. Inject: µl on column Also available in 96-well format 4.e4.6e4.e4.8e4.4e4.e4.6e4.e min App ID 6 The sample flow through the SPE bed was kept at a rate of.- ml/min during all stages of the extraction. istamine calibration curve, Corr. Coeff.=.9994 Tel: Fax: info@phenomenex.com 4 4 min Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: -- Puerto Rico Tel: Puerto Rico Tel: Questions or Requests? Contact ClinicalResearch@phenomenex.com for questions on applications or to request additional applications.

8 Metanephrine and Normetanephrine Metanephrine and Normetanephrine are metabolites of epinephrine and norepinephrine. Their measurements are key in the diagnosis of tumors of the adrenal glands such as pheochromocytoma, which can be associated with hypertension. Samples were extracted from plasma to reduce interferences and suppression using Strata -X-CW SPE. Luna ILIC PLC columns provide good retention for these polar compounds Sample Prep: Free Metanephrines (unconjugated were extracted and concentrated from plasma using mg Strata-X-CW weak cation-exchange polymeric resin in a 96-well plate Part No.: 8E-S-TGB SPE Procedure. Condition: 4 µl Methanol/Acetonitrile (:. Equilibrate: 4 µl Water. Load: µl plasma diluted with ml Water 4. Wash: 8 µl Water followed by ml Methanol/Acetonitrile (:. Dry: minutes at g 6. Elute: x µl of % Formic acid (freshly prepared in Acetonitrile/Methanol (: 7. Dry down: to dryness under stream of nitrogen at 4 C and reconstitute in µl Acetonitrile/ mm Ammonium formate, p. (9: 8. Cap: reconstituted extract immediately to prevent evaporation N Metanephrine Normetanephrine Also available in 96-well format Metanephrine and Normetanephrine LC/MS/MS Method ILIC is generally performed using mobile phases containing high concentrations (>7 v/v % of Acetonitrile, which are ideally suited for use with MS detection. Under ILIC conditions the more polar compounds elute later in the chromatographic run, which is exactly opposite to what is observed under reversed phase conditions. Analyte Q Q MN MN-d 8.4. NMN NMN-d e4 7.e4 6.e4.e4 4.e4.e4.e4.e4 7.8e4 7.e4 6.e4.e4 4.e4.e4.e4.e min. Metanephrine-d 8./. 4 min Column: Luna µm ILIC Dimensions: x. mm Part No.: B-4449-B Mobile Phase: A: Acetonitrile/ mm Ammonium formate, p. (9: B: Acetonitrile/Water/ mm Ammonium formate, p. (:4: Flow Rate:.4 ml/min Injection vol: µl of reconstituted extract Gradient: Time (min e4.e4.e4.e4.e4.e4.e4. Sample:. Metanephrine (MN. Metanephrine-d (MN-d. Normetanephrine (NMN 4. Normetanephrine-d (NMN-d.4 Normetanephrine-d 69./7. 4 min Metanephrine 8.4/48. 4 min Normetanephrine 66.4/4. App ID min Questions or Requests? Contact ClinicalResearch@phenomenex.com for questions on applications or to request additional applications. Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: -- 4 Puerto Rico Tel: Puerto Rico Tel:

9 Nicotine and its Metabolites Nicotine is the most abundant alkaloid present in all tobacco products. Determination of nicotine metabolism / pharmacokinetics provides a useful tool for estimating uptake of nicotine and tobacco-related toxicants, for understanding the pharmacologic effects of nicotine and nicotine addiction, and for optimizing nicotine dependency treatment. Tobacco products also contain other alkaloids that can serve as unique markers of tobacco use. Two examples are anabasine and nornicotine, which are present in tobacco products, but not in nicotine replacement therapies. Fast run time (<6 minutes Resolution of alkaloids that can confirm tobacco use Summary: The samples were extracted using Strata -X-C 6 mg/ ml tubes Part No.: 8B-S9-UBJ. Dilute. ml urine with. ml of mm Ammonium acetate, p 4.. Add µl internal standard. Proceed to SPE procedure SPE Procedure. Condition: ml Methanol (- ml/min. Equilibrate: ml Ammonium acetate buffer. Load:. ml diluted urine sample 4. Wash : ml Ammonium acetate buffer. Wash : ml % Methanol 6. Dry: > g for min to remove residual water 7. Elute: x. ml. % Ammonium hydroxide/methanol solution 8. Dry down: Nitrogen gas at C 9. Reconstitute: µl of Acetonitrile/ mm Ammonium bicarbonate (:9 Nicotine Anabasine Also available in 96-well format Nicotine and its Metabolites LC/MS/MS METD Nicotine, Cotinine, -ydroxycotinine, Nornicotine and Anabasine analysis ( ng/ml urine extracted standard.6e.e.4e.e.e.e.e 9.e4 8.e4 7.e4 6.e4.e4 4.e4.e4.e4.e min Analyte Q Q Nicotine 6.. Nicotine-d Cotinine Cotinine-d Nornicotine Nornicotine-d Cotinine Cotinine-d Anabasine 6.. Standard curves from ng/ml to ng/ml for Nicotine and Nornicotine. Column: Gemini NX-C8, µm Dimensions: x. mm Part No.: D-44-B Mobile Phase: A: mm Ammonium Bicarbonate B: % Acetonitrile Gradient: Time (min Flow Rate:. ml/min Injection Volume: µl Temperature: C Sample:. Nornicotine. --Cotinine. Anabasine 4. Cotinine. Nicotine App ID 6 Request our new nicotine tech note! Nicotine Nornicotine Analyte Area/ IS Area Analyte Area/ IS Area R = R = Analyte Conc./ IS Conc Analyte Conc./ IS Conc. Questions or Requests? Contact ClinicalResearch@phenomenex.com for questions on applications or to request additional applications. Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: Puerto Rico Tel: Puerto Rico Tel:

10 Methylmalonic Acid (MMA Methylmalonic Acid (MMA levels are tested to diagnose and monitor Vitamin B deficiency, which is often related to the metabolic disorder methylmalonic acidemia. Pain Control and Illicit Drug Panel Pain management drugs are among the most abused drugs today. LC/MS/MS is a key technique for determining which drugs are being abused, whether through illicit or prescribed use. minute run time and excellent peak shape using Gemini PLC columns Summary: The samples can be extracted from urine or serum using Strata -X-AW mg/ ml Part No.: 8B-S8-TAK SPE Procedure. Into individually labeled. ml conical micro-centrifuge tubes combine. ml mm Ammonium formate, µl IS and µl blank, standard, or sample. Activate the SPE cartridge with ml % Methanol. Equilibrate the SPE cartridge with ml mm Ammonium formate 4. Load sample and proceed with elution. Wash the SPE cartridge with. ml % Methanol 6. Dry the SPE bed under high vacuum for - min 7. Elute the analyte with x.6 ml % N 4 in Methanol 8. Evaporate the tubes in a concentrator at 4- ºC 9. Remove the tubes and resuspend the residue in µl.% Formic acid. Inject µl on column LC/MS/MS Method.6e.4e.e.e.8e.6e.4e.e.e 8.e4 6.e4 4.e4.e4 Column: Gemini µm C8 Dimensions: x. mm Part No.: D-449-Y Mobile Phase: A:. % Formic acid in DI Water B:. % Formic acid + mm Ammonium Formate in Methanol Gradient: Time (min min 9 9 Flow Rate:.7 ml/min Injection Volume: µl Temperature: 4 C Sample:. Succinic Acid. Methyl Malonic Acid (MMA App ID 86 Methylmalonic Acid Excellent separation of isobaric compounds Choose from PLC/UPLC stationary phases (C8 and PFP to optimize separations for your sample SPE PRCEDURE: Summary: Strata -X-Drug B µm Polymeric Strong Cation, 6 mg / 6 ml, Tube, /pk Part No.: 8B-S8-UC The solvent volumes shown below are for a 6 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.. Conditioning: No conditioning required.. Load: i. To ml urine sample, add µl of conc. Cl. ii. eat at 9 C for hours. iii. Add ml of mm Sodium Acetate Buffer (p 4.. iv. Add ml of 6 N K. Vortex. v. Centrifuge for mins at rpm. vi. Verify p of sample is between vii.. Wash: Load pre-treated sample directly onto SPE cartridge. i. ml of mm Sodium acetate (p. buffer ii. ml of Methanol 4. Elute ml of Ethyl acetate:isopropanol:n 4 (7::. Evaporate: to dryness under a stream of nitrogen at C. 6. Reconstitute: ml of % methanol. 7. Inject: μl on PLC / Mass Spectrometer (MS. For serum/plasma samples:. Pre-treatment: Dilute plasma sample : with % acetic acid. Vortex. Centrifuge for minutes at g. Load directly onto Strata-X-Drug B sorbent, and proceed to step of above SPE procedure. No conditioning required. For saliva samples:. Pre-treatment: Dilute saliva sample : with % acetic acid. Vortex. Centrifuge for minutes at g. Load directly onto Strata-X-Drug B sorbent., and proceed to step of above SPE procedure. No conditioning required. Morphine Codeine xycodone Methamphetamine N Lorezepam N Benzoylecgonine Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: Puerto Rico Tel: Puerto Rico Tel:

11 Pain Control and Illicit Drug Panel Kinetex.6 µm PFP, x. mm.e6.e6.e6 4.e6 4.e6.e6.e6.e6.e6.e6.e6.e Column: Kinetex.6 µm PFP Dimensions: x. mm Part No.: B-4477-Y Mobile Phase: A: mm Ammonium formate B: Methanol Gradient: Time (min Flow Rate:. ml/min Injection Volume: µl Temperature: 4 C min 9 9 App ID 9 Pain Control and Illicit Drug Panel Kinetex C8.6 µm, x. mm.e6.9e6.8e6.7e6.6e6.e6.4e6.e6.e6.e6.e6 Column: Kinetex.6 µm C8 Dimensions: x. mm Part No.: B-446-Y Mobile Phase: A: mm Ammonium Formate B: Methanol with. % Formic Acid Gradient: Time (min 4 4. Flow Rate:. ml/min Injection Volume: µl Temperature: Ambient 9.e 8.e 7.e 6.e.e 4.e.e.e.e min App ID 689 Resolution of Critical Isobars.84.e4.4e4.e4.e4.e4.e ID Q Q Morphine 86. xymorphone. 7 ydromorphone Codeine.. xycodone 6. 4 Amphetamine 6. 9 Naltrexone MAM ydrocodone. 99. Methamphetamine. 9. MDA 8. Benzoylecgonine Naloxone 8. MDMA Morphine/-Morphine min ID Q Q MDEA Norfentanyl. 84. Meprobamate Tramadol Normeperidine Meperidine 48. PCP Norbuprenorphine 44.. EDDP Fentanyl 7.. Flurazepam 88.. Norpropoxyphene 8.. Clonazepam 6 7. Flunitrazepam Tel: Fax: info@phenomenex.com Codeine/-Codeine.... min ID Q Q a-hydroxyalprazolam. 97 Propoxyphene Methadone. 6. Sufentanil Carisoprodol xazepam Lorazepam 7. Alprazolam 9. 8 Temazepam.. Nordiazepam 7. 4 Midazolam Buprenorphine Diazepam ID Q Q xymorphone. 7 Morphine 86. ydromorphone Amphetamine 6. 9 Methamphetamine. 9. xycodone 6. 4 MDA 8. MDMA Codeine.. Naltrexone MDEA MAM ydrocodone. 99. Benzoylecgonine Norfentanyl. 84. Tramadol Naloxone 8. Normeperidine Meperidine 48. Meprobamate PCP Norbuprenorphine 44.. EDDP Norpropoxyphene 8.. Clonazepam 6 7. Flunitrazepam a-hydroxyalprazolam. 97 Carisoprodol xazepam Methadone. 6. Lorazepam 7. Fentanyl 7.. Flurazepam 88.. Alprazolam 9. 8 Temazepam.. Propoxyphene Nordiazepam 7. 4 Diazepam Midazolam Sufentanil Buprenorphine Resolution of Critical Isobars Codeine/ydrocodone min Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: -- Puerto Rico Tel: Puerto Rico Tel: e.e.e..e.e.e..e6 9.e 8.e 7.e 6.e.e 4.e.e.e.e Morphine/ydromorphone/Norhydrocodone min xymorphone/dihydrocodeine/noroxycodone min

12 7--Progesterone 7--Progesterone Measurements of levels of 7-hydroxyprogesterone are useful in the evaluation of patients with suspected congenital adrenal hyperplasia (CA. CA leads to excess production of androgen, resulting in male characteristics to appear earlier than normal in men or more prominently than usual in females. LC/MS/MS Analysis Analyte Q Q 7--Progesterone ( (7-P- 7--Progesterone-D Excellent peak shape and linearity using Gemini NX-C8 PLC columns Summary: Strata -X-A mg/ ml SPE tubes were used to extract the samples from human serum Part No.: 8B-S-TBJ Sample Pretreatment. Dilute. ml of sample with ml DI Water and. ml IS solution ( ng/ml Testo-D and ng/ml 7--Prog-D8 in Methanol/DI Water (:. Proceed to SPE procedure 7--Progesterone. µg/l standard.4e4.e4.e4.7e4.6e4.4e4.e4.e App ID 6 Column: Gemini NX-C8 µm Dimensions: x. mm Part No.: B-44-B Mobile Phase: A:. % Formic acid in DI Water B:. % Formic acid in Acetonitrile Flow Rate:.4 ml/min Column Temp: Ambient Injection Volume: µl Gradient: Time (min Sample:. Testosterone. 7--Progesterone SPE Procedure min. Condition: ml % Methanol. Equilibrate: ml DI Water. Load: pretreated sample 4. Wash:.6 ml % Methanol. Dry: under high vacuum for - min 6. Elute: x.6 ml % Methanol 7. Evaporate: to dryness at - C under gentle nitrogen stream 8. Reconstitute: µl % Acetonitrile in. % Formic acid Also available in 96-well format Calibration Data 7--Progesterone e4 Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: -- Puerto Rico Tel: Puerto Rico Tel:

13 Steroids Panel Corticosteroids are an important class of compounds that have uses as therapeutic agents as well as illicit uses. Steroid hormones are widely used throughout the body and are involved in numerous regulatory pathways including immune response, sexual differentiation, and metabolic function. Separate steroids from serum in one run on Kinetex PLC/UPLC C8 columns Quick sample preparation using protein precipitation Summary: The analytes were extracted from µl of human serum by protein precipitation, diluted, and injected. Part No.: CE-76 For protein precipitatin method details, go to Analyte Q Q Q DEAS 7... Cortisol Deoxycortisol Androstenedione Estradiol (water loss Testosterone Progesterone DEA 7... Progesterone ydroxyvitamin D Corticosterone Testosterone Progesterone Cortisol imethod Available Contact AB SCIEX or for details AB SCIEX imethod Test for Steroid Panel Version. for Cliquid Software Steroids Panel.8E6.6E6.4E6.E6.E6 8.E 6.E 4.E.E Column: Kinetex.6 µm C8 Dimensions: x. mm Part No.: D-446-Y Mobile Phase: A: Water B: Methanol Flow Rate:. ml/min Gradient table: Time (min min Standard calibration curves (n=4 r =.9969 Testosterone.- ng/ml %CV < 7.6% % accuracy > 94.% r = Progesterone.- ng/ml Questions or Requests? Contact ClinicalResearch@phenomenex.com for questions on applications or to request additional applications. %CV < 9.8% % accuracy > 94.6% App ID r = r =.99 TIP: Consider Impact for high throughput protein precipitation. See Progesterone.- ng/ml %CV < 8.4% % accuracy > 94.4% Estradiol.- ng/ml %CV < 9.88% % accuracy > 94.%... Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: -- 4 Puerto Rico Tel: Puerto Rico Tel:

14 Total Testosterone Total Testosterone Total testosterone analysis is useful for determining testicular function in males and diagnosing abnormal physical characteristics related to bodily development. LC/MS/MS Method Analyte Q Q Testosterone Testosterone-D Excellent sample cleanup from plasma using Strata -X-A SPE Fast run time in less than minutes using Gemini NX-C8 PLC columns Testosterone.6e.4e Summary: Part No.: Total testosterone was extracted from human plasma using Strata-X-A mg/ ml SPE cartridges 8B-S-TBJ Sample Pretreatment. Dilute. ml of sample with ml DI water and. ml IS solution ( ng/ml Testosterone-D in Methanol. Proceed to SPE Procedure SPE Procedure. Conditions: ml % Methanol. Equilibrate: ml Water. Load: pretreated sample 4. Wash:.6 ml % Methanol. Dry: SPE Bed under vacuum 6. Elute: x. ml % Methanol 7. Evaporate: to dryness at - C under a stream of Nitrogen 8. Add:. ml % ydroxylamine to form oxime and heat for -6 min at 6-6 C 9. Add:. ml % Formic acid in DI Water. Inject: µl onto the column Note: The quantitation is based on the testosterone-oxime derivative See Performance Characteristics of a Novel Tandem Mass Spectrometry Assay for Serum Testosterone. Kushnir, Mark M, et al. ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 848, USA. Clin Chem :-8. 6 Jan. Also available in 96-well format e.e.8e.6e.4e.e.e.8e.6e.4e.e.e 8.e4 6.e4 4.e4.e min Calibration Curve ng/dl, ng/dl Column: Gemini NX-C8 µm Dimensions: x. mm Part No.: B-449-B Mobile Phase: A:. % Formic acid, mm Ammonium formate in DI Water B:. % Formic acid, mm Ammonium formate in Acetonitrile Column Temp: C Injection Volume: µl Flow Rate:.4 ml/min Gradient: Time (min Analytes:. Testosterone. Testosterone-D. Epitestosterone App ID 844 Questions or Requests? Contact ClinicalResearch@phenomenex.com for questions on applications or to request additional applications e4 Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: Puerto Rico Tel: Puerto Rico Tel:

15 --Vitamin D and D Vitamin D is recognized as an essential nutrient for intestinal absorption of calcium and phosphate and for promoting bone resorption and formation. Deficiency can lead to bone diseases such as rickets and osteomalacia. Vitamin D levels are measured by the total serum concentrations of the two common forms of - Vitamin D, which are - D and - D. Customer-submitted patient sample run on a multi-channel system 4- µl injection volume. Add: µl of the precipitating reagent containing internal standard to a. ml centrifuge tube. Pipette: µl of serum into a centrifuge tube. Vortex: seconds 4. Inspect: each tube to ensure no unmixed sample remains in the bottom of the tube. i. A homogeneous mixture is critical ii. If unmixed sample remains at the bottom of the tube, dislodge by inverting and tapping, then re-vortex. Centrifuge: minutes at, rpm 6. Transfer: supernatant into sample vial without disturbing the pellet Vitamin D ergocalciferol Vitamin D cholecalciferol --Vitamin D and D LC/MS/MS Analysis Analyte Q Q - D D D-d Patient Sample Chromatogram run on a multi-channel system.4e4.e e4.4e4.e4.e D - D Column: Kinetex.6 µm C8 Dimensions: x 4.6 mm Part No.: B-446-E Mobile Phase: A:. % Formic acid B: mm Ammonium acetate with. % Formic Acid in Methanol Gradient: Time (min Flow Rate: ml/min Temperature ºC Sample: -hydroxyvitamin D (- D -hydroxyvitamin D (- D -hydroxyvitamin D-d6 (- D-d min min 6.e4.e4 4.e4.e4.e4.e4 - D-d6 (IS min App ID 94 TIP: Consider Impact for high throughput protein precipitation. See Request our technical note on --VitaminD and D! TN- Questions or Requests? Contact ClinicalResearch@phenomenex.com for questions on applications or to request additional applications. Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: Puerto Rico Tel: Puerto Rico Tel:

16 --Vitamin D and -epi---vitamin D Vitamin D (Ergocalciferol, D and Cholecalciferol, D has been under intense investigations and its beneficial health effects have been associated with various treatments from treating bone deficiency to a potent anticancer agent. owever, isomerization of monohydroxy Vitamin D produces -epi (conversion of a- to ß-, a diasteromeric form. There is some ambiguity as to the clinical significance of the -epi isomer in general population and is currently under investigation. Good separation of --Vitamin D and -epi---vitamin D for accurate quantitation using Kinetex.6 µm PFP Fast run time in less than minutes --Vitamin D and -epi---vitamin D.4e6.e6.e6.8e6.6e6.4e6.e6.e6 8.e 6.e -Epi---D --D --D LC/MS/MS Analysis Column: Kinetex.6 µm PFP Dimensions: x. mm Part No.: D-4477-AN Temperature: Ambient Mobile Phase: A:. % Formic acid in DI Water B:. % Formic acid in Methanol Flow Rate:.4 ml/min Gradient: Time (min MRM Transitions Analyte Q Q -Vit D Vit D/Epi-D IS (-D Vit D (Sec trans Vit D (Sec trans Request our technical note on -epi-- Vitamin D! TN- 4.e.e min -Vit D Calibration Curve from. ng/ml App ID App ID 7 Tel: Fax: info@phenomenex.com -Vit D Calibration Curve from. ng/ml Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: -- Puerto Rico Tel: Puerto Rico Tel: App ID 6

17 Vitamin B 6 Vitamin B 6 currently refers to six biologically interconvertible -hydroxy- -methylpyridine compounds: pyridoxal (PL, pyridoxine (PN, pyridoxamine (PM, and their respective -phosphate (PLP, PNP, and PMP. f these compounds, PLP is the primary biologically active form of vitamin B 6. N P Vitamin B 6 PLC Analysis Sample chromatogram of semicarbazide derivatized PLP and PL using Gemini µm NX-C8 PLC column ( x 4.6 mm I.D. Gemini -NX PLC columns provide baseline separation of PLP, 4-PA and PL Accurately quantitate PLP and 4-PA. Thaw patient plasma samples and sera/plasma spiked calibrators or pre-manufactured calibration standards and controls at ambient temperature. Protect from light.. Pipette 4 μl of the blank (Water, calibration standards, controls and plasma specimens into the appropriate labeled. ml Eppendorf microcentrifuge tubes. i. Briefly vortex the calibrators and controls immediately prior to sampling. ii. iii. Mix the plasma samples by gentle inversion immediately prior to sampling. Protect the tubes from light Vitamin B 6 mau Column: Gemini -NX µm C8 Dimensions: x 4.6 mm Part No.: D-44-E Guard: Security Guard Cartridge C8, 4 x. mm I.D. Part No.: AJ-868 Mobile Phase: A: mm Sodium phosphate and. ml Acetic acid in DI water; p = ~6.; no p adjustment. B: (7: Acetonitrile:Methanol Gradient: Time (min Flow Rate:. ml/min Injection Volume: µl Temperature: C Fluorescence: Detection: Ex 6 Em 4 Sample:. PLP. 4-PA. PL App ID 684. Add μl of mg/ml semicarbazide/glycine solution in rapid succession into all the tubes containing samples; cap the tubes, vortex for seconds Incubate in the dark at room temperature for minutes.. Uncap the tubes; add μl of % meta-phosphoric acid to the controls and patient samples. 4 6 min 6. Recap the tubes and vortex for seconds at room temperature. 7. Centrifuge for minutes at 4, rpm. Note: The relative centrifugal force (RCF=6, g. 8. Transfer μl of supernatant to a ml amber vial. 9. Cover the vial with lid and place in the autosampler (RT. uman Serum PLP Standard Curve Example Standard Curve from 6. nmol/l to nmol/l for PLP in uman Serum (external standard method. Inject µl. 4 y = 6.44x R =.9999 Peak Area Questions or Requests? Contact ClinicalResearch@phenomenex.com for questions on applications or to request additional applications. PLP Concentration in uman Serum (nmol/l Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: -- Puerto Rico Tel: Puerto Rico Tel:

18 Vitamins Fat Soluble Bath Salts Fat soluble vitamins play an important role in healthy bone and teeth development, blood clotting, weight loss, and providing antioxidants. After consumption, fat soluble vitamins are absorbed through the intestinal tract, stored in body tissues and remain in the body longer than their water-soluble counterparts. Vitamin K Bath Salts are among the newest designer drugs that target predominantly young people while attempting to stay ahead of existing anti-drug laws. Bath salts are similar to amphetamines in their chemical composition, effects and dangers. MDPV Sample preparation. The individual vitamin samples were prepared as follows: Vitamin A-acetate ( mg/ml Dissolve mg of Vitamin A-acetate in ml of Methanol. Vitamin D ( mg/ml Dissolve mg of Vitamin D in ml of Methanol. Vitamin E (8 mg/ml Dissolve 8 mg of Vitamin E in ml of Methanol. Vitamin E-acetate ( mg/ml Dissolve mg of Vitamin E-acetate in ml of Methanol. Vitamin K ( mg/ml Dissolve mg of Vitamin K in ml of Acetonitrile (Vitamin K is not soluble in Methanol.. Each vitamin sample was diluted with appropriate solvents to mg/ml.. µl of each vitamin was mixed to yield the vitamin mix. Vitamin E Vitamin A 4.e6 4.e6.e6.e6.e6 Column: Kinetex.6 µm C8 Å Dimensions: x mm Part No.: B-446-Y Mobile Phase: A: mm Ammonium formate B: Methanol with. % Formic acid Gradient: Time (min 4 4. Flow Rate:. ml/min Temperature: Ambient Detector Info: API 4 Sample:. Methylone. Mephedrone. MDPV 4. Methamphetamine Mephedrone N N.e6 PLC Analysis.e6 Methylone mau Column: Kinetex.6 µm C8 Dimensions: x 4.6 mm Part No.: D-446-E Guard: SecurityGuard ULTRA System Part No.: AJ-9 (holder AJ-8768 (cartridges, /pk Gradient Isocratic Mobile Phase: Acetonitrile:Methanol (9: Flow Rate:. ml/min Temperature: C Detection: 8nm Analytes:. Vitamin A (trans-retinol. Vitamin A acetate. Vitamin E(alpha tocopherol 4. Vitamin D (Cholecalciferol. Vitamin E acetate (alpha tocopherol acetate 6. Vitamin K (Phylloquinone 4.e6.e min App ID 79 6 App ID min Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: -- 4 Puerto Rico Tel: Puerto Rico Tel:

19 Ethyl glucuronide (EtG and Ethyl sulfate (EtS Ethyl glucuronide (EtG and Ethyl sulfate (EtS are ethanol metabolites that indicate recent ingestion of alcohol. These metabolites can be detected in urine for up to 8 hours after ingestion, providing a more accurate tool for monitoring alcohol use and abuse compared to standard urine alcohol tests or breathalyzers. Excellent retention of EtG and EtS EtG Very Long Chain Fatty Acids by GC/MS Fatty Acid levels can be indicative of the effects of dietary intake, modifications, and supplements on one s health. f particular interest are levels of cis and trans fatty acid levels due to the link between trans fats and cardiovascular disease. pa Summary: LC/MS/MS Analysis Analyte Q Q EtG. 7. EtS The sample was diluted prior to injection. EtS App ID 98 min 7.e4 6.e4 6.e4.e4.e4 4.e4 4.e4.e4.e4.e4.e4.e4.e4. Column: Synergi. µm ydro-rp Å Dimensions: x mm ID Part No.: D-487-Y Mobile Phase: A: mm Ammonium Acetate B: Methanol:Acetonitrile (: Flow Rate: μl/min Gradient: Time (min Temperature: C Detector: API 4 Sample:. EtG (ethyl glucunoride. EtS (ethyl sulfate App ID 97 Column: SGE BPX7 Phase: 7 % cyanopropyl polysilphenyl-siloxane Dimensions: m x. mm x. µm Part No.: CG-6 ven Profile: 8 C for min to C/min for min to 7 C/min for 6 min Carrier Gas: Constant Flow elium,. ml/min Injection: Split 48:;.4 C Detection: Flame Ionization (FID ( C Liner: AG-7, 4 mm ID FocusLiner Analyst Note: Analytes are ppm in exane Analytes:. C6. C8. C 4. C. C 6. C 7. C4 8. C4: cis 9 9. C. C6. C6: cis 9. C7. C7: cis 4. C8. C8: trans 9 6. C8: cis 9 7. C8: cis 8. C8: trans 9, 9. C8: cis 9,. C8: cis 6, 9,. C8: cis 9,,. C8:4 Cis 6, 9,,. C 4. C: cis min Chromatogram and conditions courtesy of Masterfoods UK. Questions or Requests? Contact ClinicalResearch@phenomenex.com for questions on applications or to request additional applications. Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: Puerto Rico Tel: Puerto Rico Tel:

20 Columns for LC/MS/MS Separations Kinetex Core-Shell Technology Ultra igh Performance on ANY LC System Get sub- µm performance and speeds on any LC system Increase your throughput without loss of critical pair resolution Save money by using less solvent due to faster runtimes rdering Information.6 µm Analytical Columns (mm SecurityGuard ULTRA Cartridges Phases x 4.6 x x 4.6 x 4.6 x 4.6 /pk $ 79 $ 69 $ 679 $ 699 $ 749 $ 8 XB-C8 B-4496-E C-4496-E D-4496-E F-4496-E AJ-8768 C8 A-446-E B-446-E C-446-E D-446-E F-446-E AJ-8768 C8 B-4497-E C-4497-E D-4497-E F-4497-E AJ-877 PFP A-4477-E B-4477-E C-4477-E D-4477-E F-4477-E AJ-877 ILIC A-446-E B-446-E C-446-E D-446-E F-446-E AJ-877 Phenyl-exyl B-449-E D-449-E F-449-E AJ-8774 for 4.6 mm ID NEW µm Columns Available! Call for details µm. µm Solid Core. µm Porous Shell.7 µm Kinetex.7 µm Core-Shell Particle Reduced diffusion path maximizes efficiency Increased efficiencies compared to traditional fully porous sub- µm columns. Typical operating backpressures > 4 bar.6 µm MidBore Columns (mm SecurityGuard ULTRA Cartridges Phases x. x. 7 x. x. x. /pk $ 79 $ 69 $ 679 $ 699 $ 749 $ 8 XB-C8 A-4496-Y B-4496-Y C-4496-Y D-4496-Y F-4496-Y AJ-877 C8 A-446-Y B-446-Y C-446-Y D-446-Y F-446-Y AJ-877 C8 A-4497-Y B-4497-Y C-4497-Y D-4497-Y F-4497-Y AJ-8777 PFP A-4477-Y B-4477-Y C-4477-Y D-4477-Y F-4477-Y AJ-878 ILIC A-446-Y F-446-Y AJ-8779 Phenyl-exyl AJ-878 for. mm ID.9 µm Solid Core. µm Porous Shell.6 µm Fully Porous Particle Solutions Kinetex.6 µm Core-Shell Particle Reduced diffusion path maximizes efficiency Ultra-high performance on any PLC or UPLC system with Kinetex.6 µm columns.6 µm Minibore Columns (mm SecurityGuard ULTRA Cartridges Phases x. x. x. x. /pk $ 79 $ 69 $ 699 $ 749 $ 8 XB-C8 A-4496-AN B-4496-AN D-4496-AN F-4496-AN AJ-878 C8 A-446-AN B-446-AN D-446-AN F-446-AN AJ-878 C8 A-4497-AN B-4497-AN D-4497-AN F-4497-AN AJ-8784 PFP A-4477-AN B-4477-AN D-4477-AN F-4477-AN AJ-8787 ILIC A-446-AN B-446-AN D-446-AN F-446-AN AJ-8786 Phenyl-exyl B-449-AN D-449-AN AJ-8788 for. mm ID.7 µm MidBore Columns (mm SecurityGuard ULTRA Cartridges Phases x. x. x. /pk $ 79 $ 799 $ 89 $ 8 XB-C8 A-4498-Y B-4498-Y D-4498-Y AJ-877 C8 B-447-Y D-447-Y AJ-877 C8 A-4499-Y B-4499-Y D-4499-Y AJ-8777 PFP D-4476-Y AJ-878 ILIC B-4474-Y AJ-8779 Phenyl-exyl AJ-878 for. mm ID The Standard for p Method Development Rugged, fully porous particles with excellent column lifetimes p - stability allows for low and high p mobile phases to optimize selectivity for ionizable compounds.7 µm Minibore Columns (mm SecurityGuard ULTRA Cartridges Phases x. x. x. x. /pk $ 79 $ 799 $ 89 $ 869 $ 8 XB-C8 A-4498-AN B-4498-AN D-4498-AN F-4498-AN AJ-878 C8 A-447-AN B-447-AN D-447-AN F-447-AN AJ-878 C8 A-4499-AN B-4499-AN D-4499-AN F-4499-AN AJ-8784 PFP A-4476-AN B-4476-AN D-4476-AN F-4476-AN AJ-8787 ILIC A-4474-AN B-4474-AN D-4474-AN AJ-8786 Phenyl-exyl B-4-AN D-4-AN F-4-AN AJ-8788 for. mm ID SecurityGuard ULTRA cartridges require holder, Part No.: AJ-9 If you are not completely satisfied with Kinetex core-shell columns, send in your comparative data to a similar product within 4 days and KEEP TE CLUMN FR FREE. Full Range Selectivity for Reversed Phase Separation 4 unique phases to optimize even the most challenging separations Available in. µm and 4 µm particles for high efficiencies SecurityGuard ULTRA Cartridge System The SecurityGuard ULTRA cartridge system protects ultra-high performance columns, like Kinetex, from damaging contaminants and microparticulates. Extend Kinetex column lifetime Simple to use Pressure rated to, psi (,78 bar Fits virtually all manufacturers columns ne of the World s Leading PLC Columns Numerous phases including C8(, Phenyl-hexyl, PFP(, ILIC and others Available in. µm and µm particles for high efficiencies SecurityGuard ULTRA Cartridge olders Part No. Description Unit Price AJ-9 SecurityGuard ULTRA Cartridge older ea $ 97 UPLC / PLC Sure-Lok igh Pressure PEEK Male Nut Fittings Part No. Description Unit Price AQ-8 Sure-Lok igh Pressure PEEK -Pc Nut -, /pk $ 7 for /6 in. Tubing,, psi (87 bar AQ-8 Sure-Lok Fitting Tightening Tool, Aluminum ea 7 AQ-8 AQ-8 Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: Puerto Rico Tel: Puerto Rico Tel:

21 Gemini U.S. Patent No. 7, 6, 67 rdering Information µm Microbore, Minibore and Narrow Bore Columns (mm SecurityGuard Cartridges (mm Phases x. x. x. x. x. x. x. x. x. 4 x.* $ 469 $ 4 $ 4 $ 49 $ 6 $ 7 $ $ 6 $ 7 $ /pk C8 B-449-A M-449-B A-449-B B-449-B D-449-B F-449-B B-449-Y D-449-Y F-449-Y AJ-796 C6-Phenyl B-444-A A-444-B B-444-B D-444-B F-444-B B-444-Y D-444-Y F-444-Y AJ-794 $ 479 $ 48 $ 4 $ 699 $ 789 $ 4 $ 7 $ 8 $ /pk NX-C8 M-44-B A-44-B B-44-B D-44-B F-44-B B-44-Y D-44-Y F-44-Y AJ-867 for ID:.-. mm µm Analytical Columns (mm SecurityGuard Cartridges (mm Phases x 4. x 4.6 x 4.6 x 4.6 x 4.6 x x.* $ 49 $ 4 $ 49 $ 6 $ 7 $ 79 $ /pk C8 M-449-D A-449-E B-449-E D-449-E F-449-E G-449-E AJ-797 C6-Phenyl A-444-E B-444-E D-444-E F-444-E G-444-E AJ-79 $ 4 $ 699 $ 77 $ 89 $ /pk NX-C8 B-44-E D-44-E F-44-E G-44-E AJ-868 for ID:.-8. mm µm Minibore and Narrow Bore Columns (mm SecurityGuard Cartridges (mm Phases x. x. x. x. x. x. x. x. 4 x.* $ 49 $ 479 $ 64 $ 7 $ 7 $ 99 $ 689 $ 7 $ /pk C8 A-44-B B-44-B F-44-B G-44-B B-44-Y D-44-Y F-44-Y G-44-Y AJ-796 C6-Phenyl A-4444-B B-4444-B F-4444-B B-4444-Y F-4444-Y G-4444-Y AJ-794 $ 46 $ $ 79 $ $ 67 $ 76 $ 79 $ /pk NX-C8 A-444-B B-444-B F-444-B B-444-Y D-444-Y F-444-Y G-444-Y AJ-867 for ID:.-. mm µm Analytical Columns (mm SecurityGuard Cartridges (mm Phases x 4.6 x 4.6 x 4.6 x 4.6 x x.* $ 49 $ 46 $ 99 $ 68 $ 7 $ /pk C8 A-44-E B-44-E D-44-E F-44-E G-44-E AJ-797 C6-Phenyl A-4444-E B-4444-E D-4444-E F-4444-E G-4444-E AJ-79 $ $ 67 $ 74 $ 779 $ /pk NX-C8 B-444-E D-444-E F-444-E G-444-E AJ-868 for ID:.-8. mm *SecurityGuard Analytical Cartridges require holder, Part No.: KJ-48 If Gemini analytical columns do not provide at least an equivalent separation as compared to a competing column of similar particle size, similar phase and dimensions, send in your comparative data within 4 days and keep the Gemini column for FREE. Solid Phase Extraction for ptimal Clean Up Strata-X U.S. Patent No. 7,9,4 A reversed phase functionalized polymeric sorbent that gives strong retention of neutral, acidic, or basic compounds under aggressive, high organic wash conditions. Mechanisms of Retention π-π Bonding n Strata-X Ṅ. rdering Information Sorbent Format Mass Part Number Unit Price Tube mg 8B-S-TAK ml (/box $ 8 mg 8B-S-TBJ ml (/box 6 mg 8B-S-UBJ ml (/box mg 8B-S-EBJ ml (/box mg 8B-S-EC 6 ml (/box 4 mg 8B-S-FBJ ml (/box 6 mg 8B-S-FC 6 ml (/box mg 8B-S-BJ ml (/box mg 8B-S-C 6 ml (/box 7 Giga Tube mg 8B-S-DG ml (/box 7 g 8B-S-JDG ml (/box 8 g 8B-S-JEG ml (/box g 8B-S-KEG ml (/box 4 g 8B-S-LFF 6 ml (6/box 9 Teflon Tube mg 8B-S-FBJ-T ml (/box 8 mg 8B-S-FDG-T ml (/box 9 96-Well Plate mg 8E-S-AGB Plates/Box 8 mg 8E-S-TGB Plates/Box 8 6 mg 8E-S-UGB Plates/Box 8 ydrogen Bonding Dipole-Dipole Interactions n Ṅ. General Extraction Protocol Method written for a mg/ ml tube. Condition ml Methanol followed by ml DI Water. Load: Pretreated sample ydrophobic Interaction. Wash: ml -6 % Methanol in DI Water. 4. Dry: sorbent for seconds n Material Characteristics Particle Size (µm Pore Size (Å 8 Surface Area (m /g 8 p Stability -4. Elute: x µl % Formic acid in Methanol or Acetonitrile Ṅ. If Strata-X SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 4 days and keep the Strata-X SPE product for FREE! n-line Extraction Cartridge Description Part Number Unit/Box Price Strata-X on-line extraction M-S-B-CB ea $ 4 cartridge, x. mm Cartridge holder, mm C-84 ea Instant Method Development Create your wn SPE Method in under minute! Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: Puerto Rico Tel: Puerto Rico Tel:

22 Strata-X-C Strata-X-CW U.S. Patent No. 7,9,4 A strong cation-exchange functionalized polymeric sorbent that allows for complete retention of basic compounds with a pk a less than., making % organic wash conditions possible. Material Characteristics Particle Size (µm Pore Size (Å 8 Surface Area (m /g 8 p Stability -4 Ionic Capacity meq/g pk a ~ U.S. Patent No. 7,9,4 A weak cation-exchange functionalized polymeric sorbent that allows for complete retention of basic compounds with a pk a greater than 8, including quaternary amines, making % organic wash conditions possible. Material Characteristics Particle Size (µm Pore Size (Å 8 Surface Area (m /g 8 p Stability -4 Ionic Capacity.76 meq/g pk a ~ 4. Mechanisms of Retention Mechanisms of Retention Strong Cation-Exchange π-π Bonding ydrophobic Interaction Weak Cation-Exchange π-π Bonding ydrophobic Interaction = = S - + = = S - + = = S - + Strata-X-CW General Extraction Protocol Strata-X-C rdering Information Format Sorbent Mass Part Number Unit Price Tube mg 8B-S9-TAK ml (/box $ 4 mg 8B-S9-TBJ ml (/box 6 mg 8B-S9-UBJ ml (/box mg 8B-S9-EBJ ml (/box 8 mg 8B-S9-EC 6 ml (/box 7 mg 8B-S9-FBJ ml (/box 9 mg 8B-S9-FC 6 ml (/box 8 mg 8B-S9-BJ ml (/box 6 mg 8B-S9-C 6 ml (/box 4 Giga Tube mg 8B-S9-DG ml (/box g 8B-S9-JDG ml (/box g 8B-S9-JEG ml (/box 4 g 8B-S9-KEG ml (/box 48 g 8B-S9-LFF 6 ml (6/box,9 96-Well Plate mg 8E-S9-AGB Plates/Box 6 mg 8E-S9-TGB Plates/Box 6 6 mg 8E-S9-UGB Plates/Box 6 General Extraction Protocol Method written for a mg/ ml tube. Condition: ml Methanol followed by ml DI Water. Load: Pretreated sample. Wash: ml. N ydrochloric acid in DI Water followed by ml. N ydrochloric acid in Methanol 4. Elute: x µl % Ammonium hydroxide in Methanol or Acetonitrile If Strata-X SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 4 days and keep the Strata-X SPE product for FREE! rdering Information Sorbent Format Mass Part Number Unit Price Tube mg 8B-S-TAK ml (/box $ 4 mg 8B-S-TBJ ml (/box 6 mg 8B-S-UBJ ml (/box mg 8B-S-EC 6 ml (/box 7 mg 8B-S-FBJ ml (/box 9 mg 8B-S-FC 6 ml (/box 8 mg 8B-S-BJ ml (/box 6 mg 8B-S-C 6 ml (/box 4 Giga Tube mg 8B-S-DG ml (/box g 8B-S-JDG ml (/box g 8B-S-JEG ml (/box 4 g 8B-S-KEG ml (/box 48 g 8B-S-LFF 6 ml (6/box,9 96-Well Plate mg 8E-S-AGB Plates/Box 6 mg 8E-S-TGB Plates/Box 6 6 mg 8E-S-UGB Plates/Box 6 Method written for a mg/ ml tube. Condition: ml Methanol followed by ml DI Water. Load: Pretreated sample. Wash: ml DI Water followed by ml Methanol 4. Elute: x µl % Formic acid in Methanol or Acetonitrile If Strata-X SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 4 days and keep the Strata-X SPE product for FREE! n-line Extraction Cartridge Description Part Number Unit/Box Price Strata-X-C on-line extraction M-S48-B-CB ea $ 7 cartridge, x. mm Cartridge holder, mm C-84 ea n-line Extraction Cartridge Format Part Number Unit/Box Price Strata-X-CW on-line extraction M-S6-B-CB ea $ 7 cartridge, x. mm Cartridge holder, mm C-84 ea Instant Method Development Create your wn SPE Method in under minute! Instant Method Development Create your wn SPE Method in under minute! Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: Puerto Rico Tel: Puerto Rico Tel:

23 Strata-X-A Strata-X-AW U.S. Patent No. 7,9,4 U.S. Patent No. 7,9,4 A strong anion-exchange functionalized polymeric sorbent that allows for complete retention of weakly acidic compounds with pk a greater than, making % organic wash conditions possible. Material Characteristics Particle Size (µm Pore Size (Å 8 Surface Area (m /g 8 p Stability -4 Ionic Capacity. meq/g pk a ~ 4 A weak anion-exchange functionalized polymeric sorbent that allows for complete retention of acidic compounds with pk a less than, making % organic wash conditions possible. Material Characteristics Particle Size (µm Pore Size (Å 8 Surface Area (m /g 8 p Stability -4 Ionic Capacity.6 meq/g pk a ~ 9 Mechanisms of Retention Mechanisms of Retention Strong Anion-Exchange π-π Bonding ydrophobic Interaction Weak Anion-Exchange π-π Bonding ydrophobic Interaction C C C N + N + N + N N N C C C N N N Strata-X-A rdering Information Sorbent Format Mass Part Number Unit Price Tube mg 8B-S-TAK ml (/box $ 4 mg 8B-S-TBJ ml (/box 6 mg 8B-S-UBJ ml (/box mg 8B-S-EBJ ml (/box 8 mg 8B-S-EC 6 ml (/box 7 mg 8B-S-FBJ ml (/box 9 mg 8B-S-FC 6 ml (/box 8 mg 8B-S-BJ ml (/box 6 mg 8B-S-C 6 ml (/box 4 Giga Tube mg 8B-S-DG ml (/box g 8B-S-JDG ml (/box g 8B-S-JEG ml (/box 4 g 8B-S-KEG ml (/box 48 g 8B-S-LFF 6 ml (6/box, 96-Well Plate mg 8E-S-AGB Plates/Box 6 mg 8E-S-TGB Plates/Box 6 6 mg 8E-S-UGB Plates/Box 6 General Extraction Protocol Method written for a mg/ ml tube. Condition: ml Methanol followed by ml DI Water. Load: Pretreated sample. Wash: ml mm Ammonium acetate (p 6-7 followed by ml Methanol 4. Elute: x µl % Formic acid in Methanol or Acetonitrile If Strata-X SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 4 days and keep the Strata-X SPE product for FREE! Strata-X-AW rdering Information Sorbent Format Mass Part Number Unit Price Tube mg 8B-S8-TAK ml (/box $ 4 mg 8B-S8-TBJ ml (/box 6 mg 8B-S8-UBJ ml (/box mg 8B-S8-EBJ ml (/box 8 mg 8B-S8-EC 6 ml (/box 7 mg 8B-S8-FBJ ml (/box 9 mg 8B-S8-FC 6 ml (/box 8 mg 8B-S8-BJ ml (/box 6 mg 8B-S8-C 6 ml (/box 4 Giga Tube mg 8B-S8-DG ml (/box g 8B-S8-JDG ml (/box g 8B-S8-JEG ml (/box 4 g 8B-S8-KEG ml (/box 48 g 8B-S8-LFF 6 ml (6/box, 96-Well Plate mg 8E-S8-AGB Plates/Box 6 mg 8E-S8-TGB Plates/Box 6 6 mg 8E-S8-UGB Plates/Box 6 General Extraction Protocol Method written for a mg/ ml tube. Condition: ml Methanol followed by ml DI Water. Load: Pretreated sample. Wash: ml mm Ammonium acetate (p 6-7 followed by ml Methanol 4. Elute: x µl % Ammonium hydroxide in Methanol or Acetonitrile If Strata-X SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 4 days and keep the Strata-X SPE product for FREE! Instant Method Development Create your wn SPE Method in under minute! Instant Method Development Create your wn SPE Method in under minute! Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: Puerto Rico Tel: Puerto Rico Tel:

24 Strata-X-Drug B U.S. Patent No. 7,9,4 Strata-X-Drug B is a polymeric strong catiion-exchange SPE sorbent that is designed and quality controlled with Substance Abuse and Mental ealth Services Administration (SAMSA regulations in mind. Easy to Follow Methods Methods are written for 6 mg/6 ml Strata-X-Drug B; however they can be scaled to accommodate smaller or larger sample sizes and sorbent masses. piates, 6-MAM, PCP, and Amphetamines*. Condition: No conditioning required. Load: Pre-treated urine sample. Wash : ml of mm Sodium acetate buffer (p. 4. Wash : ml Methanol. Dry: minutes under full vaccum 6. Elute: ml of Ethyl acetate/isopropanol/ammonium hydroxide (7:: Marijuana Metabolites. Condition: No conditioning required. Load: Pre-treated urine sample. Wash : ml of mm Sodium acetate buffer (p. 4. Wash : ml of Acetonitrile/ mm Sodium acetate buffer (p. (:7. Dry: minutes under full vacuum 6. Elute: ml of Ethyl acetate/isopropanol (8: Cocaine Metabolites. Condition: No conditioning required. Load: Pre-treated urine sample. Wash : ml of. N ydrochloric acid 4. Wash : ml Methanol. Dry: minutes under full vacuum 6. Elute: ml of Ethyl acetate/isopropanol/ammonium hydroxide (7:: * piates, 6-MAM, PCP, and Amphetamines can be extracted simultaneously or separately using the same SPE methodology. An Analytical Support Laboratory PhenoLogix customizes solutions to fit your exact needs and requirements so methods can be transferred efficiently and effectively back into your lab upon completion. Please contact us to get started on your method. phenologix@phenomenex.com or Visit: ow can we be of service? PhenoLogix worldwide technical teams are here to provide full service method support and training for you and your lab. ur services include: Method Improvement and ptimization New Method Development Validation and Pre-validation Services n-site Training and Consulting Strata-X-Drug B rdering Information Format Sorbent Mass Part Number Unit Price Tube mg 8B-S8-AAK ml (/box $ 8 mg 8L-S8-AAK ml (/box 9 mg 8B-S8-TAK ml (/box 4 mg 8L-S8-TAK ml (/box 6 mg 8B-S8-TBJ ml (/box 6 mg 8B-S8-UBJ ml (/box 6 mg 8B-S8-UC 6 ml (/box 6 mg 8B-S8-UCL 6 ml (/box 68 Giga Tube mg 8B-S8-EDG ml (/box 96-Well Plate Tab-less tube mg 8E-S8-AGB Plates/box 6 mg 8E-S8-TGB Plates/box 6 6 mg 8E-S8-UGB Plates/box 6 If Strata-X SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 4 days and keep the Strata-X SPE product for FREE! Instant Method Development Create your wn SPE Method in under minute! Partial list of resources used in the PhenoLogix lab Agilent, Shimadzu, ACQUITY and JASC PLC and UPLC systems API LC/MS/MS API 4 LC/MS/MS API LC/MS/MS 4 Q-TRAP LC/MS/MS Waters Micromass Quattro MS Agilent and Shimadzu GC/MS systems Gilson and PerkinElmer liquid handlers Tel: Fax: info@phenomenex.com Tel: Fax: info@phenomenex.com USA Tel: -- USA Tel: Puerto Rico Tel: Puerto Rico Tel: