BIO - BERGOLIO SACHA INCHIK OIL

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Bergolio GmbH Park Hoechi Weg 13 CH-6353 Weggis Suisse

0041-41 390 4190 Fax 0041-41 390 4192 Mobile 0041-79-254

1 Bergolio GmbH Park Hoechi Weg 13 CH-6353 Weggis Suisse Switzerland Suiza Tel Fax Mobile UID: CHE Sumak Kawsay Buen Vivir E.I.R.L. Cajatambo #1405 Lima 01 Peru RUC: BIO - BERGOLIO SACHA INCHIK OIL Bergolio International FZE P.O. Box RAK, UAE(DUBAI)

2 Bergolio GmbH, Weggis Contents Introduction... 3 Sacha Inchik Taxonomic Classification... 4 Obtention of the product... 5 Essential composition and quality factors... 6 Nutrition Facts Contents of Oil Seed Oil and Protein Bio Inspecta Certificate Bergolio Sacha inchik vegetable and organic omega Vs. Fish Oil Advantages of Sacha inchik oil compared to other vegetable oils Cosmetic Use Research & Studies of Sacha Inchik... 24

3 Bergolio GmbH, Weggis Introduction For more than 3,000 years, the Plukenetia Volubilis also known as the Sacha Inchik or Inca Peanut has been used by inhabitants of the Amazon Rainforest for better total body health and increased endurance. Found in the highlands of the Andes Mountains in Peru, the plants are durable, producing year after year for decades. Now people the world over are praising this Super Food as an excellent source of essential fatty acids, proteins and amino acids more than most other currently recommended sources. It s also easier to break down, helping every cell to function at its most optimum. Your body is going to love how easily it digests the proteins and nutrients of the Sacha Inchik. Since our bodies do not produce omega-3 oils naturally, we must get them from our diet. The Inca Peanut is one of the few nuts (though they are actually seeds) that offers a perfect balance of omega-3 and omega-6 fatty acids, as well as protein, vitamin A and alpha-tocopherol vitamin E (the most beneficial form of vitamin E), while being 96% digestible and 100% irritation free. Current scientific studies indicate the Sacha Inchik oélagineux as the best in its composition and high nutritional quality: The oil has a high content of Omega 3 (more than 48.%), omega 6 (35%) will omega 9 (10.34%) Digestibility is very high (over 96%) Contains antioxidants vitamin A and vitamin E alpha-tocoferol Over 60% of defatted almond is a high quality complete protein (99% digestible) Very rich and Essential und Non-essential amino acids in sufficient quantities to health.

4 Bergolio GmbH, Weggis Sacha Inchik Taxonomic Classification The Bergolio sacha inchik Oil is extracted from selected seeds of Plukenetia Volubilis Linneo. It is a natural, additive-free, non-refined vegetable oil. Family Gender Species Scientific name Common name Euphorbiaceous Plukenetia Volubilis Linneo Plukenetia Volubilis Linneo Sacha Inchik BERGOLIO SACHA INCHIK SEED HAS POTENTIAL AS RAW MATERIAL FOR THE FOOD, NUTRACEUTICAL/SUPPLEMENT, PHARMACEUTICAL AND COSMETIC INDUSTRY.

Bergolio GmbH, Weggis - 5 - Obtention of the product Suitable

Alimentarius are used in the oil production process.

5 Bergolio GmbH, Weggis Obtention of the product Suitable mechanical pressing techniques authorized by the Codex Alimentarius are used in the oil production process. Such techniques do not alter the nature and neither the quality of the oil.

6 Bergolio GmbH, Weggis Essential composition and quality factors Ingredents: 100% Sacha Inchik Seed Oil organic Food additives: None admitted. Shelf Life: 24 Monts Storage: Keep in a cool and dry place away from light and heat Taste: like as fresh aroma Advanced Technology: The Bergolio Sacha Inchik BERGOLIO guarantees the highest quality of raw materials used BERGOLIO used light-proof packaging. The products are protected against UV rays BERGOLIO used the new Technology by extract of the oil (Vegan) Swiss-Quality: Production and packaging in Switzerland

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16 Bergolio GmbH, Weggis Nutrition Facts

17 Bergolio GmbH, Weggis Contents of Oil Seed Oil and Protein RESULTS

18 Bergolio GmbH, Weggis Bio Inspecta Certificate

19 Bergolio GmbH, Weggis Bergolio Sacha inchik vegetable and organic omega Vs. Fish Oil Bergolio oil is an organic, biological product; fish oil is exposed to contamination in the sea: dioxide, mercury, benzopyrenes and others. Because Bergolio oil is a vegetable oil it contains no cholesterol, fish oil does contain cholesterol. Bergolio oil is metabolised by the human body for its own needs in about 10% in DHA AND EPA FATTY ACIDS, fish oil is metabolised by fish for the needs of the fish.* Bergolio oil is easy to digest while fish oil is less so. Bergolio oil has a better flavour and aroma than fish oil. Bergolio oil does not cause the acidity and irritation that fish oil does. Bergolio oil does not cause intestinal gases like fish oil. Bergolio oil has a high proportion of essential fatty acids (84.41%) while fish oil has a very small essential fatty acid content 3%. Bergolio oil has a high proportion of unsaturated fatty acids (93.69%), fish oil contains less unsaturateds (65%). Bergolio oil has the lowest saturated fat content (6.39%), while fish oil has a high saturated fat content (40%). Bergolio oil has more physiological functions in the human body than fish oil. Bergolio oil is consumed raw, it is obtained from the first cold pressing, it is virgin and is not refined while fish oil for human consumption is extracted and refined at high temperature using solvents and chemicals. Bergolio oil contains important natural antioxidants such as alphatocopherol vitamin E, vitamin C carotenoids and thus, unlike fish oil, does not require artificial preservatives to be added. * Dr. Josef Pies "Sacha Inchi-das Omega-3-Öl aus der Inka-Nuss" (VAK Verlags GmbH, Kirchzarten)

Bergolio GmbH, Weggis - 20 - Advantages of Sacha inchik oil compared to other vegetable oils The plant Plukenetia

highly unsaturated oils as a world source of such oil the most important are as follows: 1.

rainforest and was used to be a wild plant, now the Government look for a trade with socio-economic and environment

Sacha Inchik is cultivated in the Amazon region and is a sustainable source of income for many womans and families of

20 Bergolio GmbH, Weggis Advantages of Sacha inchik oil compared to other vegetable oils The plant Plukenetia volubilis whose seeds are used to produce sacha Inchik oil has important advantages compared to other plants producing highly unsaturated oils as a world source of such oil the most important are as follows: 1. Biodiversity and fair trade Plukenetia Volubilis or Sacha Inchik is a plant herbaceous originally from the Amazon rainforest and was used to be a wild plant, now the Government look for a trade with socio-economic and environment responsibility. Sacha Inchik is cultivated in the Amazon region and is a sustainable source of income for many womans and families of the area through the use of ideas and traditional methods of cultivation. Sacha Inchik is a forest crop and encourages reforestation of the Amazon, growing it in the Amazon forest has a more positive impact than that of other oil plants. 2. Industrial advantages

21 Bergolio GmbH, Weggis Nutritional advantages "The Omega 3, EPA and DHA are in the seaweed, some fish of cold waters and the human organism synthesizes the fatty acids EPA and DHA by elongation processes starting from essential fatty acid linoleic and linolenic (source: Marine Villavicencio Núñez, Biochemical I, 1996)." When the human organism assimilates the essential fatty acids linoleic and a-linolenic, elongates them converting the linoleic chain from 18 atoms of carbon with 2 two double bonds into 20 atoms with 5 double bonds, producing the fatty acid Eicosapentaenoic (EPA) and the a-linolenic chain enlongates from 18 atoms of carbon with 3 double bonds into 22 atoms with 6 double bonds, producing the fatty acid Docosahexaenoic (DHA); these fatty acids are denominated Omega-3. Rich natural source of Omega 3, Omega 6 and Omega 9, incomparable for the invigoration of the cerebral functions, their consumption is ideal for people of all ages.

22 Bergolio GmbH, Weggis Economic advantages The high seed production per hectare and the high oil content oft the seeds give Bergolio Sacha Inchik considerable advantages over other oil plants used to produce oils and proteins. The easy industrial processing of Bergolio Sacha Inchik is a further advantage. 5. Health Benefits of Bergolio Sacha Inchik Bergolio Sacha Inchik can be helpful in the treatment of many other health conditions: Powers every Organ and System in Your Body Feeds your Brain improving cognitive functions Good for Mental Health Improves attention and concentration Depression Supports your Eyes and Vision Vital for a Healthy Heart Good for Metabolism Healthy blood glucose levels Asthma/ Ulcers/ Migrane Headaches/ Glaucoma/ & Others Lowers Cholesterol Hypertension Arthritis Relieves Inflammation Mood Enhancer Family and friends are nicer, people treat you with respect and courtesy, life is brighter and happier Good for Joint Health Eases stiffness improves your mobility and grip strength Vital for Bone Health Better calcium absorption and maintains bone density Important for Healthy Skin, Nails and Hair

23 Bergolio GmbH, Weggis Cosmetic Use The Sacha Inchik (Plukenetia volubilis linneo) plant is indigenous to the Peruvian Amazon Forest, and produces small nuts that are extremely rich in high quality, nutritious oil. External Use: The combination of Omega-3s, Vitamin E, Vitamin A, antioxidants, and amino acids makes Sacha Inchik Oil a wonderful external body treatment. It revitalizes, nourishes, protects, and hydrates skin, hair and nails. Sacha Inchik Oil is especially excellent for sensitive and dry skin, inflamed or irritated skin, and for repairing damaged hair. Various high-priced cosmetic brands have discovered the Sacha Inchik oil as an ingredient for exclusive cosmetic products and external use for the care of inflammatory and dry Skin. The benefit of alpha-linolenic acid and the positive effects are various: Strong cell-regenerating Activates the cells Supports fluidizing cell membranes Increases skin elasticity Sacha Inchik is a wonderful light, low viscosity oil for the care of greasy skin, which is absorbed immediately. It helps fight wrinkles and has a moisturizing effect, ideal for the care of skin, hair and lips. In other words, it s an excellent anti-aging oil! I would compose it with penetrations stimulating, unsaponifiables, phospholipids and oleic acid (from shea butter, avocado oil or lecithin), which help to get the polyunsaturated fatty acids in the skin. Due to its low content of saturated fatty acids and cornification disorders counteracting linoleic acid Sacha Inchik oil is also a wonderfully light, low viscosity oil for oily skin care, it is absorbed immediately; combined with babassu and grape seed oil moisturizing and not resting formulations are possible.

24 Bergolio GmbH, Weggis Research & Studies of Sacha Inchik EXPLOITING BIODIVERSITY: SACHA INCHIK (Plukenetia volubilis L.) AS SOURCE OF ESSENTIAL OILS INTRODUCTION A. Mohd Rani (1) and W.A. Wan Khairul Anuar (2) Sacha inchik or Inca nut (Plukenetia volubilis L.) is a wild, climbing, semi woody, perennial oleaginous plant of the Euphorbiaceae family that grows in the tropical jungles in America at altitudes between 200 and 1500 m. This legume has seeds of a lenticular shape, which are rich in oil and proteins. The seeds contain 35-60% oil and about 27% proteins rich in cysteine, tyrosine, threonine and tryptophan (Follegatti-Romero et al., 2009). Traditionally, its flour and oil from seeds are used in the preparation of different meals and beverages, roasted seeds and tender leaves are also consumed by Indian of Peru (Guillén et al., 2003). The special characteristic of Sacha inchik oil is that it contained large amount of unsaturated fatty acids (93% of the total fatty acid). Hamaker et al. (1992) found that the seeds of this plant contained about 45.2% of linolenic acid (omega-3) and 36.8% of linoleic acid (omega-6). Linolenic and linoleic acids are known as essential fatty acids because human cannot produce but obtain them in their diet. Alfa lenolenic (ALA; 18:3 n-3) and linoleic (LA, 18:2 n- 6) acids are the main essential unsaturated fatty acids obtained from vegetable oils. These fatty acids can be elongated and de-saturated into their longer chain derivates, arachidonicand eicosapentaenoic acids (EPA, 20:5 n-3) and docosahexaenoic acid (DHA, 22:6 n-3). Several studies have strongly suggested that these fatty acids are important in relation to the pathogenesis (and prevention) of coronary heart diseases and hypertension during pregnancy and breastfeeding besides exhibiting a hypocholesterolemic effect when used as food supplement. The optimal dietary intake of ALA seems to be about 2g/day or 0.6-1% of the total energy intake (Follegatti-Romero et al., 2003). Owing to these benefits, the manufacture of food supplemented with n-3 acyl groups such as infant formulas and clinical nutrition products, has increased considerably, and at the same time, has increased the demand for oil rich in the n-3 polyunsaturated acyl groups. In this context, Sacha inchi seed oil has potential as raw material for the food and nutraceutical industry. 1 MARDI, MARDI Kluang Station, Kluang, Johor ( mohdrani@mardi.gov.my) 2 Rice and Industrial Center, MARDI Headquaters, P.O Box 12301, Kuala Lumpur ( wkhairul@mardi.gov.my,)

25 Bergolio GmbH, Weggis One of the objectives of this study on this plant undertaken by MARDI was to determine fatty acids composition of locally-grown Sacha inchik oil. MATERIALS AND METHODS Planting material (seed and stem cutting) was obtained to study suitable techniques in the multiplication of Sacha inchik. Three months old Sacha inchi seedlings were transplanted on October 2008 to observe plant adaptability in Malaysia. The plant was found bearing fruit 6-7 months after transplanting. Mature fruits were collected and the kernel from seed was separated. Seed and kernel sample of Sacha inchi was sent to UNIPEC, UKM to determine total fatty acid and their fatty acid composition. Methodology of STP/Chem/A02- AOAC, was used to determine total fatty acids and IUPAC/GC were carried out to determine fatty acid composition. RESULTS AND DISCUSSION Total fatty acids of Sacha inchik were estimated to constitute about 34.2% and 26.3% in kernel and seed, respectively (Table 1). Low percentage of total fat in seed might be due to the seed coat present during the analysis. This suggested that the seed coat must be removed to obtained high percentage of total fatty acids. Table 1: The percentage of fatty acid of Plukenetia volubilis L. in kernel and seed Total Fat (%w/w) Kernel Seed Locally-grown Sacha inchik showed that the oil obtained from seed or kernel rich in unsaturated fatty acids with approximately 88-89% of the total fatty acids. The main fatty acids were α-linolenic (C 18:3n3) (range: 42-43%) followed by linoleic acid (C 18:2n6c) (range: 33-34%) and Oleic (C 18:1n9c) (10-11%) (Table 2). The result of fatty acid composition (%) in kernel and seed of Sacha inchi in the present study as compared to Follegatti-Romero et al. (2009) and Hamaker et al. (1992) is shown in Table 3. The fatty acids compositions values were in good agreement. The results indicated that Sacha inchi oil could be an important precursor source of Omega-3 fatty acids and that Sacha inchi oil could be a good source of both α-linolenic and linoleic acids.

26 Bergolio GmbH, Weggis Table 2: Fatty acids compositions (% of total fatty acids) of Plukenetia volubilis L. kernel and seed Fatty Acid Methyl Esther Kernel Seed Capric (C 10:0) Undecanoic (C 11:0) Tridecanoic (C 13:0) Myristic (C 14:0) Pentadecanoic (C 15:0) Palmitic (C16:0) Heptadecanoic (C 17:0) Stearic (C 18:0) Arachidic (C 20:0) Henicosanoic (C 21:0) Behenic (C 22:0) Tricosanoic (C 23:0) Lignoceric (C24:0) ΣSFA Myristoleic (C 14:1) Elaidic (trans) (C 18:1n9t) Oleic (C 18:1n9c) Cis-10-pentadecenoic (C 15:1) 0.04 nd Cis-11-eicosenoic (C 20:1) Palmitoleic (C16:1) Erucic (C 22:1n9) Cis-10-heptadecanoic (C 17:1) ΣMUFA Linolelaidic (trans) (C 18:2n6t) Linoleic(cis) (C 18:2n6c) у-linolenic (C 18:3n6) α-linolenic (C 18:3n3) Cis-11,14-eicosadienoic (C 20:2) Cis-8,11,14-eicosatrienoic (C 20:3n6) Cis-5,8,11,14,17-eicosapentanoic (C 20:5n3) Cis-13,16-docosadienoic (C 22:2) Cis-4,7,10,13,16,19-docosahexanoic (C 22:6n3) Nervonic (C 24:1n9) ΣPUFA Σ Unsaturated Fatty Acids SFA, saturated fatty acid; PUFA, polyunsaturated fatty acid; and MUFA, monounsaturated fatty acid.

Bergolio GmbH, Weggis - 27 - Table 3: Fatty acid composition (%) of the Sacha inchi in kernel and seed and their oil Fatty acid Locally grown L. A.

27 Bergolio GmbH, Weggis Table 3: Fatty acid composition (%) of the Sacha inchi in kernel and seed and their oil Fatty acid Locally grown L. A. composition (%) Kernal Seed Follegatti-Romero Hamaker Palmitic (C16:0) Stearic (C 18:0) Oleic (C 18:1n9c) Linoleic(cis) (C 18:2n6c) α-linolenic (C 18:3n3) Key Scientific References Guillén, M., A. Ruiz, N. Cabo, R. Chirinos, G. Pascual (2003). Characterization of Sacha inchi (Plukenetia volubilis L.) oil by FTIR spectroscopy and 1H NMR. Comparison with linseed oil. J. Am. Oil Chem. Soc. 80 : 755. Follegatti-Romero, L.A., C. R. Piantino, R. Grimaldi, F. A. Cabral (2009). Supercritical CO2 extraction of omega-3 rich oil from Sacha inchi (Plukenetia volubilis L.) seeds. Journal of Supercritical Fluids : Hamaker, B.R., C. Valles, R. Gilman, R. M. Hardmeier, D. Clark, H. H. García, A.E. Gonzales, I. Kohlstad, and M. Castro (1992). Amino acid and fatty acids profiles of the Inca Peanut (Plukenetia volubilis L.). Cereal ChemI. 69 : Nguemeni C., et al., Alpha-linolenic Acid: A promising nutraceutical fort he prevention of stroke, Pharma Nutrition. 2013;1:1-8. Surette ME. Dietary Omega -3PUFA and health: Srearidonic acid Containing seed oils as effective and sustainable alternatives to traditional marine oils. Mol Nutr Food Res. 2013:57:

Bergolio GmbH, Weggis - 28 - Thank you for giving us the opportunity to be your partner We

28 Bergolio GmbH, Weggis Thank you for giving us the opportunity to be your partner We believe organic food should be healthy, innovative and deliciours Mercedes Durrer, Founder

OMEGA PROJECT Cooperation between two committed companies ORGANIC LIPACTIVE INCA INCHI WO

OMEGA PROJECT Cooperation between two committed companies ORGANIC LIPACTIVE INCA INCHI WO Dr BERTHON Jean-Yves, CEO OMEGA PROJECT 2002 PROJECT GENESIS - José ANAYA looked for a substitution of fish Omega