Intestinal cytoplasmic lipid droplets, associated proteins, and the regulation of dietary fat absorption

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1 Purdue University Purdue e-pubs Open Access Dissertations Theses and Dissertations Intestinal cytoplasmic lipid droplets, associated proteins, and the regulation of dietary fat absorption Theresa M. D'Aquila Purdue University Follow this and additional works at: Part of the Biochemistry Commons, Cell Biology Commons, Molecular Biology Commons, Nutrition Commons, and the Physiology Commons Recommended Citation D'Aquila, Theresa M., "Intestinal cytoplasmic lipid droplets, associated proteins, and the regulation of dietary fat absorption" (2016). Open Access Dissertations This document has been made available through Purdue e-pubs, a service of the Purdue University Libraries. Please contact epubs@purdue.edu for additional information.

2 Graduate School Form 30 Updated PURDUE UNIVERSITY GRADUATE SCHOOL Thesis/Dissertation Acceptance This is to certify that the thesis/dissertation prepared By Theresa Marie D'Aquila Entitled INTESTINAL CYTOPLASMIC LIPID DROPLETS, ASSOCIATED PROTEINS, AND THE ABSORPTION OF DIETARY FAT. For the degree of Doctor of Philosophy Is approved by the final examining committee: Kimberly K. Buhman Chair Jon A. Story Kee- Hong Kim Nana A. Gletsu-Miller To the best of my knowledge and as understood by the student in the Thesis/Dissertation Agreement, Publication Delay, and Certification Disclaimer (Graduate School Form 32), this thesis/dissertation adheres to the provisions of Purdue University s Policy of Integrity in Research and the use of copyright material. Approved by Major Professor(s): Kimberly K. Buhman Approved by: Connie M. Weaver 7/20/2016 Head of the Departmental Graduate Program Date

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4 INTESTINAL CYTOPLASMIC LIPID DROPLETS, ASSOCIATED PROTEINS, AND THE REGULATION OF DIETARY FAT ABSORPTION A Dissertation Submitted to the Faculty of Purdue University by Theresa M D Aquila In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy August 2016 Purdue University West Lafayette, Indiana

5 For my parents, brother, and my adventure buddy ii

6 iii ACKNOWLEDGEMENTS This dissertation would not have been possible without the help, guidance, and encouragement of advisors, friends, and my family. First and foremost, I would like to express my sincerest gratitude to my advisor Dr. Kim Buhman. Her guidance and support helped me develop into the scientist that I am today. Thank you for always being there ready to listen, give advice, and bullshit science with me. I would also like to thank my academic advisory committee, Dr. Jon Story, Dr. Kee Hong Kim, and Dr. Nana Gletsu-Miller. Your knowledge, advice, and encouragement have been invaluable through this process. I would also like to thank the current and former lab members of the Buhman lab including, Amy Hung, Alicia Carreiro, Lenna Peterson and Dr. Aki Uchida. I m grateful for your help, advice, and some good laughs. Lastly, I would like to thank my climbing buddies, particularly Bryce Heckaman. Nothing like beers around a campfire after a hard day of climbing to keep everything in perspective.

7 iv TABLE OF CONTENTS Page LIST OF TABLES... x LIST OF FIGURES... xi LIST OF ABBREVIATIONS... xiii ABSTRACT... xv CHAPTER 1. INTRODUCTION Dietary fat, health, and disease Dietary fat structure and composition Dietary fat, fat soluble vitamins, and essential fatty acids Dietary fat and risk for disease Intestinal metabolism of dietary fat Digestion and uptake of dietary fat Fatty acid uptake and metabolism Fatty acid re-esterification Triacylglycerol synthesis Chylomicron synthesis and secretion Cytoplasmic lipid droplets and triacylglycerol storage Cytoplasmic lipid droplet metabolism General knowledge of CLD metabolism Physiological conditions promoting CLDs CLD composition Neutral and phospholipid composition General CLD associated proteins CLD connection with organelles... 16

8 v Page Roles of CLDs CLD synthesis CLD catabolism Adipose Triglyceride Lipase Comparative Gene Identification Hormone Sensitive Lipase Monoacylglycerol Lipase Putative methods of CLD catabolism CLD protein in enterocytes Physiological conditions promoting CLDs in enterocytes CLD composition Neutral and phospholipid composition Perilipins CLDs may serve multiple roles in the enterocyte CLD synthesis CLD catabolism Adipose Triglyceride Lipase Comparative Gene Identification Hormone Sensitive Lipase Monoacylglycerol Lipase Putative methods of CLD catabolism Autophagy Methods for identifying CLD associated proteins Introduction to identifying CLD associated proteins Isolation of CLD associated proteins Proteomics methods Targeted approach Global approach Confirmation of CLD associated proteins by imaging... 36

9 vi Page 1.5 Recent discoveries and new mysteries of CLD associated proteins General observation of newly identified CLD associated proteins Newly identified CLD associated proteins in enterocytes Effects of obesity on dietary fat absorption Whole animal/ physiological consequences Enterocyte specific consequences Conclusion References CHAPTER 2. CHARACTERIZATION OF THE PROTEOME OF CYTOPLASMIC LIPID DROPLETS IN MOUSE ENTEROCYTES AFTER A DIETARY FAT CHALLENGE Abstract Introduction Materials and methods Ethics statement Mice Mouse procedure for lipid droplet isolation and proteomic analysis Enterocyte Isolation CLD isolation TAG and protein analysis In solution digestion and LC MS/MS Data analysis and bioinformatics Immunofluorescence Imaging Transmission electron microscopy Cardiac perfusion fixation and TEM imaging of jejunum TEM imaging of isolated CLDs Immunoelectron microscopy Results... 67

10 vii Page Lipid accumulates in CLDs in enterocytes in response to a dietary fat challenge The CLD fraction is enriched with Plin3 and has a high TAG to protein ratio Proteins were identified in the CLD fraction through LC MS/MS Some identified proteins in the CLD fraction are associated with known lipid metabolism related functions Immunofluorescence imaging of proteins identified in the CLD fraction supports their CLD localization Discussion Acknowledgements References CHAPTER 3. DIET INDUCED OBESITY ALTERS CYTOPLASMIC LIPID DROPLET MORPHOLOGY AND ASSOCIATED PROTEINS IN RESPONSE TO AN ACUTE DIETARY FAT CHALLENGE Abstract Introduction Materials and methods Mice Generation of DIO mouse model Mouse procedure for lipid droplet isolation and proteomics analysis Enterocyte isolation CLD isolation In solution digestion and LC MS/MS Protein identification Data analysis and bioinformatics Immunofluorescence imaging Cardiac perfusion, fixation, and transmission electron microscopy Cytoplasmic lipid droplet size analysis

11 viii Page 3.4 Results Lipid accumulates in CLDs in enterocytes in response to an acute dietary fat lean and DIO mice CLDs in enterocyte of DIO mice are larger and have a different size distribution compared to lean mice in response to an acute dietary fat challenge Proteins were identified associated with isolated CLDs from lean and DIO mice in response to an acute dietary fat challenge Proteins identified associated with CLDs from lean and DIO mice are associated with a variety of biological processes Proteins associated with lipid related processes are found at differentially present levels and have been previously characterized associated with CLD in enterocytes proteomic or targeted protein analysis Plin3 and Plin2 localize to CLDs from lean and DIO mice in response to an acute dietary fat challenge Plin2 and Plin3 localize to different CLDs in different regions of the cell in DIO mice Discussion Acknowledgements References CHAPTER 4. LONG CHAIN FATTY ACYL COA SYNTHETASE 5 ABLATION DECREASES TRIGLYCERIDE STORAGE IN ENTEROCYTES AND SECRETION IN RESPONSE TO AN ACUTE DIETARY FAT CHALLENGE COMPARED TO WILD TYPE MICE Abstract Introduction Materials and methods Diets and mice Tissue imaging

12 ix Page TAG secretion High fat feeding and food intake analysis Fecal lipid analysis Results Acsl5-deficient mice accumulate less neutral lipids in CLDs in response to an acute dietary fat challenge compared to WT mice Decreased TAG secretion in Acsl5-deficient mice compared to WT mice Acsl5-deficeint mice have increased food intake without differences in body weight or fecal lipid content compared to WT mice when fed a high fat diet Nuclear localization of Acsl5 in enterocytes in response to an acute dietary fat challenge in WT mice Discussion Acknowledgments References CHAPTER 5. SUMMARY AND FUTURE DIRECTIONS Summary Future directions CLD morphology and associated proteins along the length of small intestine Changes in CLD morphology and associated proteins over time in response to an acute dietary fat challenge The role of Acsl5 in the nucleus References VITA PUBLICATIONS

13 x LIST OF TABLES Table... Page Table 1-1 Proteins identified by proteomic analysis associated with CLDs from enterocytes Table 2-1 GO Terms associated with protein classification term Table 2-2 A sub group of proteins are associated with known lipid related functions Table 3-1 Label free quantitation of proteins associated with lipid related Gene Ontology terms

14 xi LIST OF FIGURES Figure... Page Figure 2-1 Lipid accumulates in CLDs in enterocytes in response to a dietary fat challenge Figure 2-2 Isolated CLD fraction is enriched with CLD marker, Plin3, and has a high TAG to protein ratio Figure 2-3 Proteins identified by LC MS/MS and grouped by biological process and molecular function Figure 2-4 Lipid related proteins identified cluster based on predicted physical and functional interactions Figure 2-5 Immunofluorescence imaging of Plin3 and ApoA-IV demonstrates localization on or around CLDs Figure 2-6 Immunofluoresence and immunoelectron imaging of Acsl5 demonstrates localization on or around CLDs Figure 3-1 Lipids accumulate in CLDs in enterocytes from lean and DIO mice following an acute dietary fat challenge Figure 3-2 CLDs from enterocytes of DIO mice have a larger average diameter and have a larger maximum diameter compared to lean mice following an acute dietary fat challenge

15 xii Figure Page Figure 3-3 Number of proteins identified associated with isolated CLDs from enterocytes of lean and DIO mice following an acute dietary fat challenge Figure 3-4 STRING analysis of proteins associated with lipid related Gene Ontology terms highlight proteins clusters Figure 3-5 Plin3 and Plin2 localize to CLDs from lean and DIO mice in response to an acute dietary fat challenge Figure 3-6 Plin3 localizes to CLDs on the apical (AP) side of the nuclei compared to Plin2 which localizes to CLDs on the AP and basolateral (BL) side of the nuclei in DIO mice in response to an acute dietary fat challenge Figure 3-7 Proposed model Figure 4-1 Acsl5-deficient mice accumulate less neutral lipids in CLDs in response to an acute dietary fat challenge compared to WT mice Figure 4-2 Decreased TAG secretion in Acsl5-deficient mice compared to WT mice Figure 4-3 Acsl5-deficeint mice have increased food intake without differences in body weight or fecal lipid content compared to WT mice when fed a high fat diet Figure 4-4 Nuclear localization of Acsl5 in enterocytes in response to an acute dietary fat challenge in WT mice

16 xiii LIST OF ABBREVIATIONS Abbreviation Term 3BHS1 3-Beta-hydroxysteroid dehydrogenase AADAC Arylacetamide deacetylase ACS Acyl-CoA synthetase ACSL Acyl-CoA synthetase long chain AGPAT Acylglycerophosphate acyltransferase APO Apolipoprotein ATGL Adipocyte triglyceride lipase CARS Coherent anti-stokes ramen scattering spectroscopy CCT Phosphocholine cytidylyltransferase CD36 Cluster of differentiation 36 CE Cholesteryl ester CES Carboxylesterase CGI-58 Comparative gene identification-58 CIDE Cell death inducing DFF45-like effector CLD Cytoplasmic lipid droplet CM Chylomicron COPII Coat protein complex II DAG Diacylglycerol DGAT Acyl-CoA:diacylglycerol acyltransferase DHB2 -Hydroxysteroid dehydrogenase type 2 DIO Diet-induced obesity ER Endoplasmic reticulum FA Fatty acid

17 xiv Abbreviation Term FABP Fatty acid binding protein FAO Fatty acid oxidation FATP4 Fatty acid transport protein 4 FFA Free fatty acid G3P Glycerol-3-phosphate GPAT Glycerol phosphate acyltransferase Hepatocyte nuclear factor 4 alpha HSL Hormone sensitive lipase LAL Lysosomal acid lipase LCFA Long chain fatty acid LLDs Lumenal lipid droplets LPCAT Lysophosphatidylcholine acyltransferase MAG Monoacylglycerol MGAT or Mogat Acyl-CoA:monoacylglycerol acyltransferase MGL Monoacylglycerol lipase MTP or Mttp Microsomal triglyceride transfer protein PC Phosphatidylcholine PCTV Pre-chylomicron transport vesicle PL Phospholipid PLIN Perilipin Peroxisome proliferator-activated receptor alpha SAR1B GTPase Secretion associated Ras related 1B GTPase SNARE Soluble NSF attachment receptor TAG Triacylglycerol TEM Transmission electron microscopy

18 xv ABSTRACT D Aquila, Theresa M. Ph.D., Purdue University, August Intestinal Cytoplasmic Lipid Droplets, Associated Proteins, and the Regulation of Dietary Fat Absorption. Major Professor: Kimberly Buhman. Dietary fat provides essential nutrients, contributes to energy balance, and regulates blood lipid concentrations. These functions are important to health, but can also become dysregulated and contribute to diseases such as obesity, diabetes, and cardiovascular disease. The small intestine absorbs dietary fat through an efficient multi step process of digestion, uptake, metabolism, and secretion or storage. When dietary fat is taken up by the absorptive cells of the small intestine, enterocytes, it can be secreted into circulation where it contributes to blood lipid levels or temporarily stored in cytoplasmic lipid droplets (CLDs). The objective of this dissertation is to investigate the presence and metabolism of CLDs in the intestine. Dietary fat absorption by the small intestine is a multi-step process that regulates the uptake and delivery of essential nutrients and energy. One step of this process is the temporary storage of dietary fat in cytoplasmic lipid droplets (CLDs). The storage and mobilization of dietary fat is thought to be regulated by proteins that associate with the CLD; however, mechanistic details of this process are currently unknown. In this study we analyzed the proteome of CLDs isolated from enterocytes harvested from the small intestine of mice following a dietary fat challenge. In this analysis we identified 181

19 xvi proteins associated with the CLD fraction, of which 37 are associated with known lipid related metabolic pathways. We confirmed the localization of several of these proteins on or around the CLD through confocal and electron microscopy, including perilipin 3, apolipoprotein A-IV, and acyl-coa synthetase long-chain family member 5. The identification of the enterocyte CLD proteome provides new insight into potential regulators of CLD metabolism and the process of dietary fat absorption. We also investigated the effect of diet induced obesity on CLD morphology and associated proteins. It is currently unclear what effect diet induced obesity (DIO) has on the balance between dietary fat storage and secretion. Specifically, there is limited knowledge of how DIO affects the level and diversity of proteins that associate with CLDs and regulate CLD dynamics. In the current study, we characterize CLDs from lean and DIO mice through histological and proteomic analyses. We demonstrate that DIO mice have larger intestinal CLDs compared to lean mice in response to dietary fat. Additionally we identified 375 proteins associated with CLDs in lean and DIO mice. We identify a subgroup of lipid related proteins which are either increased or unique to the DIO CLD proteome. These proteins are involved in steroid synthesis pathways, TAG synthesis, and lipolysis. This analysis expands upon our knowledge of the effect of DIO obesity on the process of dietary fat absorption in the small intestine. Lastly, we investigated the role of one of the newly identified CLD associated proteins on dietary fat absorption. Little is known of the role of Acsl5 in the metabolism of dietary fat in enterocytes. To understand the role of Acsl5 in dietary fat absorption, we challenged WT and Acsl5-deficient mice with acute and chronic dietary fat challenges. Despite no evidence of quantitative fat malabsorption in the absence of Acls5, we found

20 xvii significantly reduced TAG storage and secretion by enterocytes compared to WT mice. Interestingly, Acsl5-deficient consumed more food, but did not gain more weight compared to WT mice during two weeks of HFD feeding. Furthermore, we found that Acsl5 localizes to the nucleus after an acute dietary fat challenge. Together this data suggests that although Acsl5 is not essential for quantitative dietary fat absorption, it plays a role in intestinal metabolism of dietary fat. The results presented in this dissertation provide new knowledge of regulators of CLD metabolism. The long term objectives of these studies are to identify mechanistic details of the process of intestinal dietary fat absorption. Understanding the process of dietary fat absorption will provide new treatments for the prevention of diseases associated with dyslipidemia.

21 1 CHAPTER 1. INTRODUCTION 1.1 Dietary fat, health, and disease Dietary fat structure and composition Dietary fat represents a diverse group of chemical compounds with different structures, functions, health benefits, and risk for disease. Dietary fat is a broad classification for lipid compounds which are consumed[1]. Dietary fat can be found from both animal and plant sources and is a significant source of energy. In the western diet, dietary fat accounts for approximately 35% of calories consumed. The predominant form of dietary fat consumed is the form of long chain triacylglycerol (TAG), which is composed of 3 fatty acids esterified to a glycerol backbone. Dietary fat also contains smaller amounts of cholesteryl esters (CE) and phospholipids (PL). Fatty acids are present within TAG, CE, or PL and consist of a chain of carbons of various chain lengths and degree of saturation. Fatty acid species include saturated fatty acids, monounsaturated fatty acids, polyunsaturated fatty acids (including omega 6 and 3 fatty acids) and trans-fatty acids. Portions of this section have been previously published in: T. D'Aquila, Y.H. Hung, A. Carreiro, K.K. Buhman, Recent discoveries on absorption of dietary fat: Presence, synthesis, and metabolism of cytoplasmic lipid droplets within enterocytes, Biochimica et biophysica acta, 1861 (2016)

22 Dietary fat, fat soluble vitamins, and essential fatty acids Dietary fat plays a critical role in health by promoting the absorption of essential nutrients of fat soluble vitamins and essential fatty acids[2]. Vitamins A (in the form of retinol esters), vitamin D, vitamin E, and vitamin K, are fat soluble vitamins whose absorption is dependent on the presence of fat in the diet. These vitamins enter the circulation as components of chylomicrons [2] [3]. Malabsorption of these vitamins may lead to deficiency and ultimately xerophthalmia, rickets, neuromuscular disorders, hemorrhaging. Essential fatty acids are also absorbed by the intestine and secreted into circulation [4]. Essential fatty acid deficiency, while rare, can result in neurological and cognitive development problems. The small intestine plays an essential role in the absorption of fat soluble vitamins, essential fatty acids, and promotion of health Dietary fat and risk for disease Dietary fat plays a critical role in the etiology of cardiovascular disease [5]. The association of diet composition and risk for coronary heart disease has been extensively studied for the last 50 years. Despite intense investigation, the link between dietary fat and coronary heart disease is a contested subject. In 1977, public health officials recommended to limit intake of dietary fat to less than 30% of daily calories in an effort to reduce the risk for coronary artery disease[6]. To assess the impact of these recommendations, The Women's Health Initiative Randomized Controlled Dietary Modification Trial, tested low fat (20% kcal from fat)/high carbohydrate intervention diet against a usual diet (>32% kcal from fat) group. This study demonstrated no effect of

23 3 reducing dietary fat on risk for coronary artery disease and cardiovascular disease [7]. This lack luster result shifted the attention from total dietary fat to dietary fatty acid composition. Diets high in saturated fat have been implicated in an increased risk for coronary artery disease. Dietary intervention trials which reduced saturated fat or replaced saturated fat with other fatty acid types reduced cardiomorbidity and cardiovascular events, but no had effect on cardiovascular mortality [8]. Based on numerous intervention trials and meta-analyses, the National Heart Foundation of Australia recommended reducing saturated and trans-fat intake and increasing omega 3, omega 6, and monounsaturated fatty acid intake, to reduce the risk of cardiovascular disease [9]. The United States has recently changed dietary guidelines to help Americans eat a healthier diet. The 2015 dietary guidelines suggest limiting the intake of calories from saturated fat to less than 10% of total calories. Additionally, the dietary guideline advisory committee suggests replacing saturated fat with polyunsaturated fat to reduce the risk for coronary artery disease [10]. More specifically, current guidelines suggest reducing trans fats and saturated fats to 1 and 7% respectively and replacing these fats with polyunsaturated and monounsaturated fatty acids [11]. Understanding the molecular mechanisms by which the small intestine absorbs dietary fat is critical for the prevention and treatment of diseases associated with dyslipidemia.

24 4 1.2 Intestinal metabolism of dietary fat Digestion and uptake of dietary fat The small intestine absorbs dietary fat through an efficient multi step process of digestion, uptake, metabolism, and secretion or storage. Dietary fat absorption is an extremely efficient process (greater than 98%) even when large amounts of dietary fat are consumed [12]. Disruption of the process of dietary fat digestion, uptake, metabolism, and secretion results in fat malabsorption as indicated by steatorrhea or presence of fat in the feces. Dietary fats are digested and emulsified in the lumen of the small intestine and ultimately taken up by enterocytes through the apical brush border membrane. Crude emulsion of dietary fat enters the small intestine from the stomach and is combined with bile acids from the gall bladder/liver and pancreatic juices. In the lumen of the small intestine, dietary fat is emulsified into mixed micelles with bile acids and phospholipids and undergo hydrolysis by pancreatic lipase [13]. The digestion of dietary TAG results in the production of free fatty acids (FFA) and sn-2 monoacylglycerol (MAG) [14] that are emulsified with bile generating mixed micelles and presented to the brush border membrane of enterocytes.

25 Fatty acid uptake and metabolism FFA and MAG are taken up by enterocytes however the mechanisms through which this occurs is not completely clear. MAG passively diffuses across the plasma membrane of enterocytes [15]. FFA on the other hand may be taken up by the enterocytes by passive diffusion or by protein mediated uptake [16]. FFA may diffuse passively across the plasma membrane via a flip flop mechanism. Since long chain fatty acid (LCFA) uptake by diffusion is a saturable process [17], a protein dependent mechanism has been proposed to promote cellular uptake of FFA. FFA may be facilitated across the plasma membrane by CD36, by enterocytes in the proximal section of the small intestine. Isolated enterocytes from the proximal section of CD36 deficient mice have decreased rate (50%) of fatty acid uptake [18]. In particular, CD36 has a high affinity and promotes absorption of very long chain fatty acids [19]. FFA may be facilitated across the plasma membrane by fatty acid transport protein 4 (FATP4). FATP4 is present on the apical side of the enterocyte and when overexpressed, increases the uptake of LCFA [20]. Interestingly, both CD36 and FATP4 deficient mice have no fat malabsorption. It has been suggested that CD36 and FATP4 may play important however non-essential roles in regulating dietary fat absorption. Although the mechanism through which fatty acids are taken up is unclear, the intestine is capable of responding to high lipid load as demonstrated by low fecal lipid content in healthy humans. FFA may be metabolically trapped in enterocytes via fatty acid binding proteins and directed toward specific metabolic fates. The upper region of the small intestine expresses two fatty acid binding proteins (FABP), FABP1 or liver FABP and FABP2 or

26 6 intestinal FABP. Both FABP1 and FABP2 are highly expressed in the small intestine and have a high affinity for LCFA [21]. It is hypothesized that FABP1 and FABP2 bind to FFA in enterocytes and limit the toxic effects of FFA [22]. FABP1 has high binding affinity for fatty acids but also other lipid species such as lysophospholipids, MAG, fatty acyl-coa, and prostaglandins [23]. FABP1 deficient mice have increased deposition of lumenally derived MAG into PL, MAG, and diacylglycerol (DAG) and with a decreased deposition in TAG. This data indicates FABP1 directs dietary MAG toward TAG synthesis. FABP1 deficient mice have a decrease deposition of lumenally derived fatty acids into mucosal oxidation indicating FABP1 directs fatty acids toward oxidation [24]. FABP2 on the other hand has a high binding affinity for fatty acids but has not been shown to have non fatty acid ligands. FABP2 deficient mice have increased deposition of lumenally derived fatty acids into PL and decreased into TAG indicating FABP2 directs fatty acids into TAG synthesis and away from PL synthesis. Interestingly, both FABP1 and FABP2 deficient mice do not have fat malabsorption as indicated by steatorrhea on chow or high fat diets. While the roles of these two proteins in the intestine is not completely clear, FABP1 and FABP2 may play a role in fatty acids transport and targeting of fatty acids towards different metabolic fates [25]. FFA may be metabolically trapped in enterocytes by conversion to fatty acyl-coa and directed toward specific metabolic fates. The thioesterification of LCFA to a long chain fatty acyl-coa is catalyzed by long chain fatty acyl-coa synthetases (ACSLs) and is an obligatory step in the cellular lipid metabolism. There are at least 25 different acyl- CoA synthetases with different tissue distribution patterns [26]. The primary ACSLs found in the intestine are Fatp4 and Acsl5. Acyl-CoA synthetases can promote cellular

27 7 fatty acid uptake via vectorial acylation [27]. Vectorial acylation has not been investigated in the small intestine, however FATP4 inhibition in isolated enterocytes by antisense oligonucleotides reduces the uptake of LCFA [20]. The role of Acsl5 in fatty acid uptake in enterocytes is unknown. In addition to driving fatty acid uptake, ACSLs have been proposed to partition fatty acids toward specific metabolic fates [28]. ACSL partitioning of fatty acids has been characterized in other cell types but has not been fully investigated in enterocytes. Acsl5 directs fatty acyl-coa toward TAG synthesis in a cell model of hepatocytes [29]. The role of Acsl5 in intestinal dietary fat absorption has not been thoroughly investigated. Initial report of Acsl5 deletion in mice shows a 60% decrease in jejunal ACSL activity but no difference in TAG secretion in response to an acute dietary fat challenge [30]. Intestinal deletion of FATP4 does not affect TAG secretion or fecal lipid content in response to an acute dietary fat challenge, however ifatp4 deficient mice have increased fecal lipid TAG content when placed on a Western diet when compared to wild type mice [31]. The investigators report no changes in expression of other FATPs in ifatp4 deficient mice, however Acsl5 expression was not measured and potentially compensates for loss of ifatp4. These results indicate highly expressed ACSLs in the intestine are dispensable for dietary fat absorption Fatty acid re-esterification Fatty acids are re-esterified generating a TAG molecule through the glycerol-3- phosphate (G3P) and acyl-coa: monoacylglycerol acyltransferase (MGAT) pathways. The first step in the G3P pathway is the acylation of G3P, which is catalyzed by glycerol-

28 8 3-phosphate acyltransferase (GPAT) enzymes and yields lysophosphatidic acid [32]. In the small intestine, two isoforms of GPAT are expressed: GPAT3 (high expression, jejunum) and GPAT4 (moderate expression) [32-34]. Gpat3-deficient mice have decreased TAG secretion and increased TAG storage in enterocytes following a dietary fat challenge [33]. The lysophophatidic acid formed during this first reaction is then acylated by acylglycerolphosphate acyltransferase (AGPAT) enzymes, also known as lysophosphatidic acid acyltransferases, to form phosphatidic acid. In the small intestine, AGPAT2, AGPAT4 or AGPAT8 may contribute to the formation of phosphatidic acid. Lipin enzymes, or phosphatidic acid phosphatases (PAPs), then remove the phosphate group to form DAG. Lipin-3 mrna is present in the small intestine of mice and humans [35]. The presence of players in the G3P pathway and results found in Gpat3-deficient mice highlight that this pathway may play a more important role in intestinal TAG metabolism than previously recognized. Alternatively, free fatty acids may be re-esterified by the MGAT pathway. MGAT activity accounts for 70-80% of TAG generated for secretion [36]. Multiple MGAT enzymes have been identified and are differentially expressed in tissues and species. The first step of the MGAT pathway for TAG synthesis is the acylation of MAG by fatty acyl-coa to produce DAG, which is catalyzed by MGAT activity (reviewed in [37]). MGAT activity is highest in the proximal small intestine and progressively decreases in distal regions [38]. Three MGAT isoforms have been identified, but only MGAT2 and MGAT3 are present in the small intestine [39]. MGAT2 is abundant in the intestine of both mice and humans, with both mrna and protein levels reflecting MGAT activity along the length of the small intestine [38]. MGAT3, on the other hand, is expressed in

29 9 humans, but not mice, and is highest in the ileum [40]. The role of MGAT2 in dietary fat absorption has been investigated in mouse models. Mgat2 protein and mrna levels increase in response to a high fat diet, suggesting this enzyme contributes to the increase in MGAT activity that occurs during dietary fat absorption [38]. Two different Mogat2- deficient mouse models were generated to understand the function of Mgat2 in intestinal TAG synthesis and dietary fat absorption [41, 42]. Although these mice still synthesize TAG in enterocytes and secrete CM-sized lipoproteins, they exhibit a reduced postprandial triglyceridemic response and a decreased TAG secretion rate in response to an oral fat load compared to wild-type mice [41, 42]. In addition, they remain efficient at absorbing dietary fat when fed a high fat diet. These results suggest that although other enzymes must be present to catalyze this activity, the fate of TAG synthesized in enterocytes may be different in Mogat2-deficient mice than wild-type mice. In fact, while less TAG is secreted in the Mogat2-deficient mouse models, more TAG is found in CLDs of jejunal enterocytes in Mogat2-deficient mice fed a high fat diet than in wild-type mice [41]. Similar effects have been observed in intestine-specific Mogat2-deficient mice [43] and in mice treated with an MGAT2 inhibitor [44]. In addition, restoring Mogat2 expression specifically in the intestine of Mogat2-deficient mice normalized the TAG absorption rate [45]. Together these results suggest that MGAT2 is not critical for absorption, but important for balancing intestinal TAG storage and secretion. Whether DAG is generated via G3P or MGAT pathway, the final step in TAG synthesis is catalyzed by Acyl- CoA: diacylglycerol acyltransferase (DGAT).

30 Triacylglycerol synthesis Acyl Co-A: diacylglycerol acyltransferase (DGAT) catalyzes the final step in TAG synthesis. Two proteins with DGAT activity have been identified and investigated in intestinal lipid metabolism. DGAT enzymes catalyze the acylation of DAG, resulting in the formation of TAG [37, 39]. This reaction is the final, committed step of TAG synthesis, and is the point at which the MGAT and G3P pathways converge. Evidence of DGAT activity on both the luminal (latent activity) and cytosolic (overt activity) sides of the ER in rat liver microsomes suggests that distinct pools of TAG destined for storage and secretion may be present in lipoprotein-producing cells [46]. Total DGAT activity is highest in the proximal intestine and decreases distally along the length of the intestine [47]. The two known DGAT enzymes, DGAT1 and DGAT2, are both integral ER membrane proteins and are both present in the small intestine. DGAT2 has also been shown to localize to CLDs and associate with mitochondria in other cell types [48, 49]. Although both of these enzymes catalyze the same reaction, they are members of two different gene families, and differ from one another in terms of structure, expression pattern, and biochemical properties. This suggests that DGAT1 and DGAT2 may play unique roles in synthesizing TAG for storage versus secretion in enterocytes Chylomicron synthesis and secretion TAG can be packaged onto chylomicrons (CM) for secretion from enterocytes. CMs are the major TAG rich lipoprotein produced by enterocytes in response to a meal containing dietary fat (reviewed in [2, 50]). CMs are made by a two-step pathway within

31 11 the ER and are then secreted via pre-cm transport vesicles (PCTVs) through the Golgi and secretory pathway on the basolateral side of the enterocyte to lymph [51]. First, TAGs and CEs are synthesized by enzymes that localize to the ER membrane. These neutral lipids are packaged onto apolipoprotein (APO) B48 with the help of microsomal triglyceride transfer protein (MTP or Mttp) [52]. APOB48 serves as a lipid acceptor and structural protein of CMs. MTP serves as a lipid transfer protein and chaperone for maintaining APOB48 structure. The neutral lipids, TAGs and CE, are surrounded by a PL monolayer containing free cholesterol and proteins. This initial structure is referred to as the primordial CM [53]. The second step in the synthesis of pre-cms is the expansion of the CM core by TAG [54]. The mechanisms underlying the addition of TAG is unclear but it is known that MTP is required, and apob-free ER lumenal lipid droplets (LLDs) are the most likely source of TAG for core expansion [55, 56]. One possible mechanism for this expansion is the fusion of LLDs with primordial CMs. Alternatively, it has been suggested that hydrolysis and re-esterification of LLDs may provide lipids for the second step of core expansion during CM assembly [55]. Pre-CMs exit the ER and are transported to the Golgi for further processing. These particles are transported within PCTVs containing coat protein complex II (COPII). COPII is made up of five core proteins: secretion associated, Ras related 1B (Sar1B) GTPase, Sec23, Sec24, Sec13, and Sec31 [56, 57]. The budding of PCTVs from the surface of the ER is mediated by liver fatty acid binding protein [58, 59] and protein kinase C isoform zeta [60]. PCTVs are larger than protein transport vesicles (250 nm versus nm, respectively) and also contain the unique vesicle-associated membrane protein 7 [61]. After budding, the PCTV fuses with the cis-golgi [62]. Within the Golgi,

32 12 pre-cms are further modified by addition of APOAI [63] and glycosylation of APOB48 [64]. Mature CMs are then destined for secretion where they are released from the basolateral side of the enterocyte by exocytosis to the lamina propria as imaged by transmission electron microscopy [65, 66]. The contractile movement of lacteals mediates the process of CM drainage to mesenteric lymphatic vessels [67]. CMs are then transported via the thoracic duct that empties into circulation at the left subclavian vein. In addition to the nonexchangeable APOB48, several exchangeable apolipoproteins can be found on the surface of CMs, including APOAI, APOAII, APOAIV, APOC and APOE [68]. The importance of the role in dietary fat absorption in health is highlighted by human congenital disorders where proteins chylomicron synthesis and secretion are defective or deficient [69]. Deficiencies in ApoB, Mttp, and Sar1b result in hypobetalipoproteinemia, abetalipoproteinemia, and chylomicron retention disease respectively. These diseases are characterized by fat malabsorption presenting as steatorrhea, low blood lipid (TAG and CHOL) levels, deficiencies in fat soluble vitamins and essential fatty acids and ultimately failure to thrive Cytoplasmic lipid droplets and triacylglycerol storage Alternatively to secretion in CM, dietary TAG can be stored in CLDs within enterocytes. The partitioning of TAG into CM synthesis has been well characterized and less is known regarding the synthesis and catabolism of CLDs in enterocytes. The balance between TAG storage and secretion regulates the appearance of TAG in the

33 13 blood. The molecular mechanisms of CLD metabolism in enterocytes are relatively unknown. Identification of mediators of intestinal CLD metabolism has the potential to significantly impact the dietary fat absorption and ultimately health and disease. 1.3 Cytoplasmic lipid droplet metabolism General knowledge of CLD metabolism Physiological conditions promoting CLDs Cytoplasmic lipid droplets are the main site of lipid storage in eukaryotic cells. CLDs are efficient stores of excess energy and are generated when nutrients are present in excess. Almost every cell type is capable of generating CLDs, however the size, number, composition, and metabolism of CLDs varies based on cell type and physiological conditions. While CLD characteristics differ, there are some common features. This section will highlight general characteristics of CLD biology and metabolism and the following sections will focus on CLDs specifically in the small intestine CLD composition CLDs are composed of a neutral lipid core, surrounded by a phospholipid monolayer and a coat of associated proteins. Additionally, CLDs may be physically connected or closely associated with other organelles which contribute to the metabolism

34 14 and function of CLDs. The composition of CLD components vary based on cell type and physiological condition. For example, CLDs in adipocytes are long term energy stores while CLDs in hepatocytes and enterocytes are more transient stores for excess fatty acids. Additionally, the presence, composition, and metabolism of CLDs may affect cell, tissue, and organism, physiology and function Neutral and phospholipid composition CLDs are composed of a neutral lipid core with a phospholipid monolayer coat. The neutral lipid core is predominantly TAG and sterol esters however additional hydrophobic compounds have been detected in isolated CLDs albeit at much lower levels. These lipid species include retinyl esters, wax esters, and ether lipids [70, 71]. The surface of the CLD is comprised of phospholipids, mainly phsophstidylcholine, phosphatidylethanolamine, and phosphatidynlinositol [70]. This amphipathic interface allows for the interaction with proteins and other organelles [72] General CLD associated proteins Various proteins associate with the CLD and regulate CLD metabolism. Perilipin (Plin) was the first protein identified associated with the CLD in 1991 [73]. Since the initial discovery of Plin, other members of the perilipin family have been identified across species with similar sequence and ability to bind to CLDs [74]. The members of the perilipin family include, Plin1 (Perilipin), Plin2 (Adipocyte differentiation related protein or ADRP), Plin3 (47 kda mannose 6-phosphate receptor-binding protein or TIP-

35 15 47), Plin4 (Adipocyte protein S3-12, or S3-12), and Plin5 (Lipid droplet-associated protein PAT-1 or OxPAT). It is still unclear what role the different Plin family members play in regulating CLD metabolism. While members of the Plin family are often characterized as regulators of CLD catabolism, new research suggest a potential role in glucose homeostasis [75, 76]. A large number of studies have been performed to identify additional proteins associated with CLDs from a variety of cell types under various physiological conditions. Fueled by technological advances and association of CLDs with various metabolic disorders, the field of CLD biology has advance dramatically in the last 10 years. A current gap in the field revolves around the targeting proteins to CLDs. Proteins may embed directly into the CLD by a hydrophobic hairpin motif or associate with the surface of the CLD by amphipathic helices or hydrophobic domains [77]. Additionally the ability of a protein to bind to CLDs may change during CLD growth and metabolism [78]. During lipolysis, CLD surface area decreases resulting in protein crowding and ultimately the removal of proteins which are more loosely bound to the CLD. The removal of proteins during CLD shrinkage has the potential to alter CLD composition and metabolism. Understanding which proteins associate with CLDs during CLD synthesis, growth, and catabolism remains an active of research [72].

36 CLD connection with organelles CLDs are highly connected organelles that physically interact with ER, other CLDs, mitochondria, and lysosomes [79]. CLDs are thought to emerge from the ER and may maintain a physical connection or close contact with the ER [80]. The bridge between ER and CLDs has been suggested to facilitate the transport of proteins and lipids between the two organelles [49] [81]. This is also a proposed mechanism of CLD growth. Additionally, CLDs are connected to other CLDs through CIDE proteins. Both CIDEC and CIDEA localize to CLD contact sites [82, 83]. CLDs are also connected or closely associated with mitochondria. Fatty acids mobilized from CLD stores can channel to mitochondria for oxidation [84], although the proteins which mediate the interaction between the two organelles remain unknown. CLDs are connected to autophagosomes. Electron microscopy images demonstrate that autophagosomes surround the CLD during starvation conditions in hepatocytes to mobilize stored TAG [85]. The process of lipid mobilization through the process of lipophagy will be described later. Recognition of CLDs as highly connected organelles is transforming the concept of CLDs from mere lipid storage sites to central players in whole cell metabolism Roles of CLDs CLDs store excess energy and help protect cells from free fatty acid induced cytotoxicity. Tissues specialized in energy storage, such as adipose tissue, can store incredible amounts of energy in the form of TAG and stored in CLDs. A non-obese person can store over 500,000 kj of energy as TAG in CLDs of adipocytes, enough to run

37 17 about 30 marathons [72]. Additionally, CLDs protect cells against toxic effects of free fatty acids. Excess non esterified free fatty acids or cholesterol can trigger cell membrane damage and trigger inflammatory responses. When esterified to TAG or CE and stored in CLDs, excess fatty acids are buffered against the cellular environment and serve as a source of lipid for cellular processes. CLDs serve as a source of fatty acids for cell function. Fatty acids mobilized from CLD stores can be used to provide energy for the cell through oxidation. Fatty acids mobilized from CLDs can be used to generate complex lipids. Fatty acids can be used for synthesis of PL, CE, or TAG. CLDs may also serve as a source of fatty acids or other lipid species functioning as signaling molecules. Adipose triglyceride lipase (ATGL), a cytosolic lipase that localizes to the CLD, catalyzes the hydrolysis of a FFA from stored TAG in the lipid droplet. The resulting FFA potentially acts as a ligand for peroxisome proliferator- activated receptor alpha (PPARa). Liver specific knockdown of ATGL decreases the expression of PPARa target genes and the effect is reversed when PPARa agonists are administered [86]. More recently, CLDs have been hypothesized to serve as protein storage sites. The most well characterized example of CLDs serving as protein depot is during embryogenesis in Drosphilia. Histones localize to CLDs until needed for cell replication [87]. Alternatively, CLDs may serve as a site for targeting proteins for degradation. In particular ApoB has been shown to localize to CLDs and aggregate when proteasomal degradation is inhibited [88]. Lastly, proteins may transiently localize to CLDs to be transferred to other organelles [89].

38 CLD synthesis CLDs are generated and expand through multiple mechanisms. There are several proposed models of CLD biogenesis, including ER budding, bicellar excision, and vesicle formation (reviewed in [90]). The model with the most current support is ER budding. In the ER budding model, the accumulation of neutral lipids within the ER membrane bilayer eventually leads to the budding of a CLD, with the cytosolic ER membrane leaflet forming its PL monolayer. This budding process may be driven solely by lipid accumulation, or mediated by proteins that bind to the ER membrane. A model of CLD synthesis with less support, the bicellar model, involves neutral lipid accumulation in the ER membrane followed by excision of this lipid along with both surrounding membrane leaflets. The issue with this model is that it would result in a transient hole in the ER membrane. Another model of CLD synthesis with less support is the formation of CLDs within bilayer vesicles. This mechanism would be mediated by vesicle formation machinery of the secretory pathway, and the lipid accumulation could occur either within the ER or after the vesicle buds. The precise mechanism of CLD synthesis in the small intestine has not been directly investigated. Once CLDs are formed, they are able to continue to accumulate TAG and increase in size. One mechanism of CLD growth is through TAG synthesis locally at the CLD surface [49, 90]. PL synthesis is important for CLD monolayer expansion in this growth mechanism. A second mechanism for CLD growth is through fusion of CLDs [90, 91]. Although there is not much known about CLD growth in enterocytes specifically, it may occur through one or both of these mechanisms.

39 19 TAG synthesis is known to occur at the ER membrane, but more recently this process has been observed at the CLD surface and shown to contribute locally to the growth of CLDs (reviewed in [90]). For this to occur, TAG synthesis machinery needs to localize to the CLD surface. One isoform of each of the enzymes involved in the G3P pathway of TAG synthesis (GPAT4, AGPAT3, PAP, and DGAT2) has been shown to localize to CLDs in either Drosophila S2 cells, 3T3-L1 adipocytes, or COS7 cells [48] [49] and [92]. In addition, specific isoforms of enzymes that activate FAs to form acyl- CoAs for incorporation into TAG (ACSL1, ACSL3, and ACSL4) have been identified on CLDs in several cell types (reviewed in [93]). In enterocytes, TAG synthesis enzymes that have been identified on CLDs are ACSL3, ACSL4, ACSL5, GPAT3 and MGAT2 in mice and humans (reviewed in [33] and [94]). Of these, ACSL3 [95], ACSL5 [96], and GPAT3 [33] have been validated by confocal or electron microscopy to be present on CLDs in enterocytes. During CLD growth, additional PLs are required for expansion of the PL monolayer surrounding CLDs to prevent their coalescence [97]. Phosphatidylcholine (PC) is the most abundant PL in these structures, and plays an important role in CLD growth [97, 98]. Three pathways mediate PC synthesis and are present in most cell types. These pathways include the de novo, or Kennedy pathway, the phosphatidylethanolamine methyl transferase (PEMT) pathway (liver cells only), and the Lands Cycle.

40 CLD catabolism Lipolysis mediates the biochemical hydrolysis of neutral lipids and CLD catabolism. The biochemical reaction of lipolysis is catalyzed by enzymes that hydrolyze ester bonds of lipids including TAGs, CEs, and PLs. TAGs are hydrolyzed to glycerol and FAs, CEs are hydrolyzed to free cholesterol and FAs and PLs are hydrolyzed to lysophospholipids and FAs. In a cell, lipolysis often refers to the breakdown of CLDs; however, lipolysis can take place wherever lipids containing ester bonds are present. Through lipolysis, the neutral lipids in the core of CLDs are able to be mobilized to a variety of metabolic fates. There are two identified pathways that mediate lipolysis of CLDs in cells. Cytoplasmic TAG lipolysis is the most well-known pathway and has been defined primarily by studies in adipocytes (reviewed in [99]). Lipophagy is a newly-identified pathway and refers to the selective degradation of CLDs through autophagy (reviewed in [100]). The enzymes and co-activator involved in cytoplasmic TAG lipolysis and lipophagy are present and associate with CLDs in multiple cell types Adipose Triglyceride Lipase ATGL catalyzes the first step of cytoplasmic lipolysis by hydrolyzing an ester bond in TAG to generate DAG and FA [101]. TAG storage in CLDs inversely correlates with ATGL activity in enterocytes. In enterocytes of C. elegans, reducing ATGL activity via RNA interference leads to increased TAG storage in CLDs, whereas increasing

41 21 ATGL activity by overexpression decreases TAG storage in CLDs [102]. In addition, whole body Atgl-deficient mice have a severe defect in lipolysis and abnormal accumulation of TAG in CLDs in many tissues (including enterocytes), and they develop obesity [103] Comparative Gene Identification- 58 CGI-58 is a cofactor that regulates ATGL activity and is present in intestine of humans, mice and C. elegans. CGI-58 binds to ATGL and is required for efficient ATGL activity; however, it has no measurable hydrolase activity [104]. Mutations in CGI-58 in humans cause Chanarin Dorfman Syndrome [105]. Chanarin Dorfman Syndrome is a rare genetic disease resulting in ichthyosis and abnormal lipid accumulation in multiple tissues including the intestine [106]. CGI-58 also plays an important role in mouse physiology, including intestinal lipid metabolism. Whole body Cgi-58-deficient mice die shortly after birth due to a severe skin barrier defect [107] Hormone Sensitive Lipase HSL has broad substrate specificity; but is thought to primarily catalyze the second step of TAG hydrolysis, the hydrolysis of an ester bond in DAG to generate MAG and FA. HSL also possesses hydrolytic activity towards TAGs, MAGs, CEs and retinoid esters, but lacks phospholipase activity [reviewed in [108]). Whole body Hsl-deficient

42 22 mice accumulate DAGs in adipose and non-adipose tissues but do not develop obesity [109, 110]. The link between HSL deficiency and human disease is not clear; however, a recent clinical study identified a frameshift mutation in the LIPE gene (encodes HSL) that reduces adipose tissue lipolysis in both basal and stimulated states [111] Monoacylglycerol Lipase MGL catalyzes the final step of cytoplasmic lipolysis by hydrolyzing MAG to glycerol and FA. MGL localizes not only to CLDs, but also cell membranes and the cytoplasm [112]. Beyond hydrolyzing dietary MAG species, MGL also hydrolyzes the endogenous cannabinoid 2-arachidonylglycerol and is known to regulate cannabinoid signaling in the central nervous system (reviewed in [113]). Therefore, effects of altered MGL expression may be due to either TAG hydrolysis or cannabinoid signaling, and are challenging to differentiate. Mouse models deficient in MGL have a decreased rate of lipolysis and accumulation of MAG [114] Putative methods of CLD catabolism Several lines of evidence suggest the presence of additional lipases particularly in non-adipose tissue (Reviewed in [115]). In the liver, ATGL accounts for less than 50% of TAG hydrolysis [116] and ATGL deficient mice develop hepatosteatosis [103]. Residual TAG activity in ATGL deficient mice and normal VLDL production suggest the presence of additional TAG hydrolases in the liver. While putative lipases have been briefly studied in the liver, very little is known about putative lipases in other cell types.

43 Arylacetamide deacetylase In addition to classical lipases and mediators of autophagy, several putative lipases have recently been identified. Arylacetamide deacetylase (Aadac) is an enzyme with mrna expression in hepatocytes, intestinal mucosa, pancreas, and adrenal glands [117]. Aadac has sequence homology with hormone sensitive lipase. In the intestine Aada mrna levels increased with fenofibrate administration. In the liver Aadac is characterized as a putative microsomal triglyceride lipase [118]. HuH7.5 cells, a human hepatoma cell line, expressing a short hairpin RNA targeting Aadac has impaired TAG lipolysis and VLDL secretion. This data indicates that Aadac may play a role in TAG lipolysis in non--adipose cell types Carboxylesterase Members of the carboxylesterase family are potential lipases. Carboxylesterase 3 (Ces1d, or triglyceride hydrolyze) has triglyceride hydrolase activity [119]. Liver specific deletion of Ces3 results in reduced plasma TAG without hepatosteatosis [120]. Other members of the carboxylesterase family have not been investigated for their role in TAG lipolysis Autophagy Autophagy is the catabolic process that breaks down cellular components sequestered within double-membrane organelles called autophagosomes by transporting them to lysosomes. Emerging studies identify CLDs as a selective substrate for

44 24 autophagy which are delivered by autophagosomes to lysosomes, where lysosomal acid lipase (LAL) hydrolyzes ester bonds in lipids. This process is referred to as lipophagy (reviewed in [100]). LAL is expressed ubiquitously and is responsible for hydrolyzing ester bonds in TAGs and CEs within the lysosome, generating free cholesterol, glycerol and FAs. Mutations in LAL in humans result in two diseases: Wolman disease (complete loss of LAL) and CE storage disease (CESD; partial loss of LAL) [121, 122]. Patients with Wolman disease usually have failure to thrive during infancy due to severe gastrointestinal issues including vomiting, diarrhea and malabsorption. Massive storage of TAGs and CEs are observed in the liver and small intestine of these patients[123]. Whole-body Lal-deficient mice have similar features of Wolman disease. These mice can survive into adulthood, but have a shorter lifespan than wild-type mice [124] CLD protein in enterocytes Physiological conditions promoting CLDs in enterocytes CLDs are present in the small intestine when chylomicron synthesis is inhibited and in response to a high fat meal. CLDs are present in the intestine when there are deficiencies in proteins involved in chylomicron synthesis including ApoB, Mttp, and Sar1b result in hypobetalipoproteinemia, abetalipoproteinemia, and chylomicron retention disease. These diseases are characterized by accumulation of TAG in enterocytes which results in steatorrhea due to enterocyte turnover. These diseases are also characterized by low blood lipid (TAG and CHOL) levels, deficiencies in fat soluble vitamins and essential fatty acids and ultimately failure to thrive. Additionally, CLDs are

45 25 present in the intestine when chylomicron synthesis and release is inhibited by chemical inhibitors. A commonly used non-ionic hydrophobic surfactant known as Pluronic L-81 reversibly inhibit the transport of intestinal chylomicrons [125]. Pluronic L-81 treated mice accumulate TAG in enterocytes in response to a meal, however it is not clear if TAG accumulates in CLD or in other cellular organelles such as the ER or the Golgi. Lastly, CLDs are transiently present in the intestine after a high fat meal. TAG levels in enterocytes increase and decrease over time [126] CLD composition Neutral and phospholipid composition CLDs in enterocytes contain neutral lipids, phospholipids, and highly hydrophobic lipids. Isolated CLDs contain primarily TAG but also cholesterol ester, diacylglycerol ether, and phospholipid, when Caco-2 cells were exposed to mixed micelles containing 2 mm sodiumtaurocholate, 0.6 mm oleic acid, 0.2 mm lysophosphatidylcholine, 0.05 mm cholesterol and 0.2 mm 1-O-octadecyl-rac-glycerol [127]. In a separate experiment CLDs were isolated from the duodenum after a cholesterol (2%) and sunflower oil challenge. The lipid content of these isolated CLDs were analyzed by lipidomics and found to contain triglyceride, cholesterol ester, free cholesterol, phosphatidylcholine, sphingomyelin, ceramides, free fatty acids and mono and di-acylglycerols. Not surprisingly, the lipid species found in isolated CLDs correspond to substrates or products of the enzymes which associate with the CLD [128].

46 Perilipins Members of the perilipin family associate with CLDs in enterocytes. The first proteins identified associated with CLDs in enterocytes were members of the PLIN family, perilipin 2 (Plin2, also adipocyte related protein (ADRP) or adipophilin)) and Plin3 (also Tip47) [129]. These proteins were investigated because the PLIN family of proteins is well established to associate with CLDs and regulate lipid storage and hydrolysis in many cell types [130]. Plin2 and Plin3 are the only members of the family identified in intestine and are differentially expressed and localize to CLDs in enterocytes based on specific dietary conditions. Plin3, an exchangeable CLD associated protein, and is absent from CLDs during fasting, as well as in chronic high-fat fed mice in the fed state, as determined by immunofluorescence microscopy [129]. The role of Plin2 in dietary fat absorption was further investigated in a Plin2- deficient mouse model [131]. Plin2-deficient mice have increased fecal TAG content and decreased CLDs in jejunum and ileum when fed either low-fat or high-fat diets compared to wild-type mice. Although the effects of Plin2-deficiency on CM synthesis and secretion were not investigated, these results indicate that Plin2 plays a role in dietary fat absorption, and Plin3 cannot fully compensate for the loss of Plin2 in this process.

47 CLDs may serve multiple roles in the enterocyte CLDs may protect enterocytes from free fatty acid toxicity[132].clds may help regulate the rate and amount of dietary fat absorption after a meal by serving as a sink and store of dietary TAG. After a meal, dietary fat can go toward chylomicron synthesis or toward storage in CLDs. Additionally, CLDs may regulate the length of the post prandial period by controlling the mobilization of stored TAG for chylomicron synthesis. CLDs may regulate the rate of steroid synthesis. Several proteins associated with steroid metabolism have been identified and confirmed on the CLD in enterocytes [94] including hydroxysteroid dehydrogenase 2 (DHB2), and Oestradiol 17- -dehydrogenase 11 (HSD17B11). While the small intestine is not typically classified as a steroidogenic organ, the presence of steroid hormone metabolism proteins associated with the CLD suggests local production or metabolism of steroids. The FAs or lipids released from enterocyte CLDs have the potential to serve as signaling molecules that regulate intestinal and/or systemic physiology. These FAs or lipids may serve as ligands for transcription factors, or substrates for synthesizing specialized, bioactive lipids that regulate diverse cellular functions. In the small intestine, FAs and lipids act as ligands for trans! ""#! (see details in Section 5.3). In addition, arachidonic acid is the major FA precursor for synthesizing eicosanoids (such as prostaglandins, leukotrienes, and other related molecules) and endocannabinoids (anandamide and 2-arachidonoylglycerol). Eicosanoids and arachidonic acid metabolites in the intestine have been shown to mediate

48 28 inflammatory processes and play a role in inflammatory bowel disease [133, 134]. Furthermore, endocannabinoids have been shown to activate the endocannabinoid system by binding to their receptors in the intestine and other cell types, resulting in protective effects against inflammation [135] and [136], regulation of food intake [137], and regulation of energy homeostasis [138]. Therefore, the dynamic CLD storage in enterocytes may be important in the regulation of lipid-mediated signaling pathways. -oxidation to generate ATP. Although glutamine and glutamate are the major fuel sources in enterocytes [ ] and intestinal FAO activity is low in fed and fasted states [142, 143], emerging studies highlight that regulation of intestinal FAO activity has the capacity to alter enterocyte TAG storage and secretion. Altering FAO in the intestine may influence systemic FA availability, which could alter postprandial blood TAG levels and/or energy balance CLD synthesis The method of CLD biogenesis in enterocytes is thought to be the result of TAG accumulation within the ER leaflet. The mechanism through which TAG is partitioned to chylomicron synthesis or CLD synthesis is unknown however may be influenced by DGAT1/2 and MGAT2 as reviewed in [37].

49 CLD catabolism In enterocytes, the size and number of CLDs increase and then decrease over time after dietary fat is consumed [126]. This depletion of CLDs over time suggests active lipolysis during dietary fat absorption. In addition, an in vitro experiment showed that the cytoplasm exhibits the highest lipolytic activity compared to other fractions in Caco-2 cells [144], indicating that CLD catabolism is an important function of intestinal lipid metabolism. CLD catabolism may regulate the quantity and rate at which dietary fat is absorbed Adipose Triglyceride Lipase Intestine-specific Atgl-deficient (iatgl-deficient) mice were generated to investigate the role of ATGL in intestinal TAG metabolism and dietary fat absorption [145]. iatgl-deficient mice have reduced TAG hydrolase activity in enterocytes from the proximal small intestine and increased TAG storage in CLDs compared with wild-type mice fed either chow or a high fat diet. However, iatgl-deficient mice have similar TAG secretion from enterocytes and no fat malabsorption compared to wild-type mice. iatgldefici regulating fatty acid oxidation (FAO), oxidative stress response, and cholesterol absorption in enterocytes compared to wild- genes in iatgl-deficient mice may contribute to their intracellular TAG accumulation, as

50 30 well as their delayed cholesterol absorption. Overall, these results suggest that intestinal ATGL directs FAs released from TAG stored in CLDs towards oxidation mediated by activation, and is not directly involved in CM synthesis for TAG secretion into circulation Comparative Gene Identification-58 Intestine-specific Cgi-58-deficient (icgi-58-deficient) mice have a significant reduction in intestinal TAG hydrolase activity, which results in massive TAG storage in CLDs in enterocytes compared to wild-type mice when fed chow or a high fat diet [146]. Unlike iatgl-deficient mice, the TAG accumulation in enterocytes of icgi-58-deficient mice leads to steatorrhea, indicating fat malabsorption. In addition, icgi-58-deficient mice have lower postprandial blood TAG levels and reduced intestinal FAO activity compared to wild-type mice. These results suggest that CGI-58 is involved in mobilizing FAs stored in CLDs that are used for both secretion and oxidation. Recently, lipid droplet protein 1, a homolog of CGI-58, was identified in enterocytes of C. elegans and found to activate lipolysis by binding to ATGL during fasting [102] Hormone Sensitive Lipase HSL is expressed along the length of the intestine in mice and primarily regulates intestinal cholesterol metabolism. Whole body Hsl-deficient mice have mild reductions in DAG hydrolase activity in the intestine but totally abolished CE hydrolase activity [147]. Mice with intestine-specific HSL deficiency (ihsl-deficient mice) were generated to

51 31 study the role of HSL in intestinal lipid metabolism [148]. ihsl-deficiency has little effect on TAG metabolism in the intestine. ihsl-deficient mice have similar TAG secretion into circulation and intracellular lipid species (including TAG, DAG, FA and PL) in enterocytes compared to wild-type mice. However, HSL-deficiency has a significant effect on intestinal cholesterol metabolism [148]. ihsl-deficient mice have higher levels of CEs, enhanced cholesterol uptake and reduced cholesterol biosynthesis in enterocytes compared to wild-type mice. These results suggest that in the intestine HSL is not required for TAG metabolism, but plays an important role in CE hydrolysis, which is required for normal intestinal cholesterol metabolism Monoacylglycerol Lipase Several mouse models with altered Mgl expression have been generated to study the role of this enzyme in intestinal lipid metabolism. Whole body Mgl-deficient mice have significantly less MAG hydrolase activity and massive accumulation of MAG species in multiple tissues, including small intestine [149, 150]. Whole body Mgldeficient mice are either leaner compared to wild-type mice fed either a low or high fat diet [149], or of similar body weight with improved glucose tolerance and insulin sensitivity [150]. In addition, these mice display blunted intestinal TAG secretion without fat malabsorption, as determined by fecal fat content [149]. They also have reduced mrna levels of genes involved in the G3P pathway and increased levels of genes involved in the MGAT pathway in enterocytes. Overall, the alterations in lipid metabolism in the absence of Mgl may contribute, in part, to the lean and/or improved

52 32 glucose and insulin sensitivity phenotype observed in these models. Mice with intestinespecific overexpression of MGL (imgl overexpression mice) have higher intestinal MGL activity, resulting in decreases in MAG species in the intestine compared to wild-type mice [151]. imgl overexpression mice are hyperphagic and have reduced energy expenditure, which contributes to increased susceptibility to obesity when fed a high fat diet. This phenotype is probably due to altered endocannabinoid signaling in the intestine since imgl overexpression mice have little changes in intestinal lipid metabolism. Intestinal TAG synthesis, metabolism of MAG into different lipid species, and intestinal FAO activity are similar in imgl overexpression mice compared to wild-type mice. However, imgl overexpression mice have significantly greater TAG accumulation in the intestine compared to wild-type mice when fed a high fat diet, but do not have fat malabsorption, as determined by fecal fat content. Whether this intestinal TAG accumulation has effects on dietary fat absorption or contributes to the obese phenotype is unclear Putative methods of CLD catabolism Carboxylesterase Members of the carboxylesterase family are putative or potential lipases. Carboxylesterase 2 is a (Ces2) is a highly expressed esterase in the liver and the intestine [152]. Recombinant mouse Ces2 demonstrates TAG hydrolase activity and liver lysates

53 33 overexpressing Ces2 have increased TAG hydrolysis [153]. Members of the Ces2 family have been identified on intestinal CLDs [96, 127] and [95] however the role of Ces2 in the intestinal lipid metabolism has not been investigated Autophagy CLDs are catabolized in the enterocyte through autophagy. Lipophagy is stimulated in Caco-2 cells in response to treatment with lipid micelles, as determined by the co-localization of TAG and components of autophagy machinery using confocal microscopy [154]. Although little is known about mechanistic activation and regulation of lipophagy in enterocytes, inhibition of autophagy initiators/mediators by mirna and drug inhibition of LAL result in an increase in TAG storage in CLDs in Caco-2 cells. Together these results highlight that lipophagy is active and regulates intestinal CLD metabolism during dietary fat absorption. 1.4 Methods for identifying CLD associated proteins Methods for identifying CLD associated proteins by either targeted or global proteomic approaches vary from study to study based on cell type of interest and endpoint, however have some consistent key features. Briefly, cells are isolated and lysed, CLDs are isolated via centrifugation, and proteins are identified by Western Blot or tandem mass spectrometry analysis. Identifying proteins associated with CLDs help identify regulators of CLD metabolism and expand the model of the role of CLDs in cell biology and physiology.

54 Introduction to identifying CLD associated proteins There are multiple methods for identifying proteins which associate with CLDs. In general, these methods fall into one of two categories, targeted and global proteomic approaches. Both of these approaches depend on isolation of CLDs without contamination of associated organelles, associated membranes, or non-cld lipid containing particles Isolation of CLD associated proteins To identify CLD associated proteins, cells are harvested under conditions where CLDs are present. The cells are lysed using various lysis methods based on cell type as described in [155]. The method of cell lysis and homogenization is dependent on the CLD size and cell type. A French Press or nitrogen bomb can be used to disrupt cells. Excess force or mechanical shearing may disrupt very large CLDs as found in adipocytes. The low density of CLDs allows the organelles to float in an aqueous layer after centrifugation. The speed at which cells are centrifuged is dependent on CLD size. Larger CLDs may be isolated at much slower speeds than smaller CLDs. In the case of lipoprotein producing tissues such as enterocytes and hepatocytes, a slower centrifugation step may allow for the separation of CLDs from lipoprotein sized contaminating particles. To identify bona fide CLD associated proteins, some investigators wash the isolated CLD fraction to remove contaminating membranes. This washing process has been shown to result in the removal of both contaminating proteins as well as reduce the level of bona fide proteins [156]. Once isolated, the CLD fraction is delipidated and proteins are

55 35 precipitated and prepared for mass spectrometry analysis [Reviewed in[157]] Once a protein is identified associated with the CLD fraction by mass spectrometry analysis, the localization of the protein to the CLD should be confirmed using imaging techniques Proteomics methods Proteomic methods for identifying CLD associated proteins fall into two general categories, targeted and global proteomics Targeted approach CLD associated proteins can be identified through a targeted proteomic approach. This approach involves the analysis of isolated CLD associated proteins by Western Blot analysis. This approach is cost effective, relatively simple, and does not require special equipment or analysis software. The downside to a targeted approach is the analysis is limited by previous knowledge of proteins which are thought to play a role in CLD metabolism or localize to CLDs Global approach CLD associated proteins can be identified through a global proteomic approach. This approach involves the analysis of CLD associated proteins by mass spectrometry analysis. This approach is more complex and requires specialized software and analysis equipment. This approach is more sensitive than traditional Western Blot analysis,

56 36 particularly with modern high resolution mass spectrometers. Within the category of global proteomics there are two sub classifications, labeled and label free proteomics. Several labeling methods exist [158] which revolves around using stable isotopes to label amino acids to quantitatively compare levels of proteins in two or more treatment groups. Alternatively, quantitative levels of CLD associate proteins can be determined using label free proteomic approach [159]. This approach utilizes normalization software to determine the relative levels of a protein in a sample Confirmation of CLD associated proteins by imaging Recent advances in imaging techniques have expanding upon the ability to visualize CLDs under different physiological conditions and resolutions. Commonly used microscopy techniques to visualize lipids are fluorphore labeled lipid (ex. BODIPY labeling of fatty acids), Coherent anti-stokes ramen scattering spectroscopy (CARS), and transmission electron microscopy. [Reviewed in [160]]. Each technique has benefits and limitations which should be taken into consideration when interpreting results. A number of techniques have been employed to visualize the localization of proteins to CLDs including conjugating proteins to fluorescent tags and conjugating proteins to fluorescently or gold labeled antibodies for confocal or TEM analysis respectively. High resolution microscopy is required to determine if a protein localizes to CLDs or surrounding structures.

57 Recent discoveries and new mysteries of CLD associated proteins General observation of newly identified CLD associated proteins A significant body of work has been generated regarding the identification of new CLD associated protein. Newly identified CLD associated proteins are associated with a wide range of biological processes and metabolic pathways. These proteins include various mitochondrial, ER, chaperone, membrane trafficking, and carbohydrate metabolism related proteins. Non-lipid related proteins have been identified in CLD proteomic profiles from multiple species and cell types [96, 156, ]. The identification of the roles of these non-lipid related proteins on the CLD is currently an understudied and underexplored area of research within the field of CLD biology Newly identified CLD associated proteins in enterocytes The proteomes of isolated CLDs derived from Caco-2 cells and mouse enterocytes have been analyzed using a tandem mass spectrometry approach [95, 96, 127] and have been previously reviewed [94]. These studies identified both known and novel CLD associated proteins. Proteins were categorized based on their gene ontology into lipid related and non-lipid related proteins. Within the lipid related category, proteins fall into various sub groupings including: modifiers of FAs, PL synthesis enzymes, lipid transporters, and steroid synthesis enzymes (Table 1-1). Several of these proteins were confirmed on the CLD through imaging techniques, including 3-beta-hydroxysteroid dehydrogenase (3BHS1) [95, 127], ACSL3 [95], ACSL5 [96], APOA- -

58 38 hydroxysteroid dehydrogenase type 2 (DHB2) [95], and LPCAT2 [95, 127]. It is currently unclear what function many of these proteins serve on the CLD; however, the presence of these proteins can be used to generate several intriguing hypotheses. 1.6 Effects of obesity on dietary fat absorption The effect of obesity on intestinal lipid metabolism is currently unclear, but may be dysregulated by obesity and associated diseases (recently reviewed [2]). Much of the research on obesity and blood fat levels have been conducted in the fasted state, however after work done by Zilversmit et al [168] which correlated increased post prandial response with an increased risk for atherosclerosis, more attention has been paid to blood fat in response to a meal. It is often to distinguish between the effect of obesity, and its comorbidity insulin resistance, on post prandial lipidemia.\ Whole animal/ physiological consequences Obesity is associated with dyslipidemia in the post prandial state which has been suggested as a risk factor for coronary artery disease [169]. The small intestine contributes to blood lipid levels in the post prandial state by secreting chylomicrons. The level of fat in the blood after a meal is a balance between the secretion of lipoproteins and clearance by peripheral tissues. The effect of obesity on this balance is relatively unknown.

59 39 Obese subjects with high levels of visceral adipose tissue had a prolonged or higher postprandial triglyceride response compared to lean subjects [ ]. The authors conclude that the postprandial response was due to in part by the contributions of liver lipoproteins [170] Enterocyte specific consequences Obesity affects lipid metabolism in enterocytes. Duodenal explants from insulin resistant obese patients had a decreased triglyceride rich lipoprotein production compared to insulin sensitive obese patients [174]. DIO mice had similar TAG content in intestinal villi in response to a dietary fat challenge as determined by CARS microscopy compared to lean mice. Additionally, DIO mice have decreased rate of TAG secretion in response to a dietary fat challenge [175, 176]. Chronic high-fat diet consumption in mice results in DIO and alters intestinal CLD metabolism. DIO mice have a reduced TAG secretion rate in response to dietary fat compared to lean mice [4] and [197]. In the fed state, DIO mice have abundant TAG storage in CLDs within enterocytes, but after a six hour fast this TAG storage is significantly reduced. In DIO mice given an acute dietary fat challenge, mrna levels of genes involved in lipolysis (Atgl and Hsl) are significantly reduced compared to before the challenge [4]. This is a response to dietary fat that does not occur in lean mice. These results suggest that DIO induces adaptations in CLD metabolism resulting in decreased mobilization of enterocyte TAG stores and decreased TAG secretion.

60 Conclusion Identifying regulators of intestinal CLD metabolism can provide potential targets for the prevention and treatment for chronic diseases associated with dyslipidemia including obesity, Type II Diabetes, and cardiovascular disease (Pirillo 2014) (Patsch 1992). The small intestine is the source of chylomicrons in the post prandial state. The balance of dietary TAG storage and secretion may regulate post prandial TAG levels. The proteins that associate with CLDs regulate CLD metabolism and TAG mobilization. Identifying proteins that associate with CLDs and regulate CLD metabolism are potential targets for regulating chylomicron lipidation and post prandial hypertriglyceridemia. A few of the outstanding questions regarding the role of CLDs, and associated proteins in the regulation of dietary fat absorption that this thesis intends to address are: 1) What proteins associated with CLDs in enterocytes in response to a dietary fat challenge? (Chapter 2). 2) What effect does obesity have on CLDs and associated proteins? (Chapter 3) 3) What role does Acsl5 play in dietary fat absorption? (Chapter 4)

61 41 Table 1-1 Proteins identified by proteomic analysis associated with CLDs from enterocytes.gene symbol Protein name Biological pathway Reference CYB5R3 NADH cytochrome b5 reductase 3 Cholesterol metabolism 95, 96, 127 DHCR7 7-Dehydrocholesterol reductase Cholesterol metabolism 127 NSDHL NAD(P)H steroid dehydrogenase-like protein Cholesterol metabolism 95, 127 ACSL3 Long chain acyl-coa synthetase 3 Fatty acid metabolism 95, 127 ACSL4 Long chain acyl-coa synthetase 4 Fatty acid metabolism 127 ACSL5 Long chain acyl-coa synthetase 5 Fatty acid metabolism 95, 96 ECHS1 Enoyl-CoA hydratase, mitochondrial Fatty acid oxidation 127 Hadh Hydroxyacyl-coenzyme A dehydrogenase Fatty acid oxidation 96 Ugt1a7c UDP-glucuronosyltransferase 1 7C Hormone metabolism 96 Bpnt1 3(2), 5-bisphosphate nucleotidase 1 Hydrolase 96 Ces2a Pyrethroid hydrolase Hydrolase 96 Ces2c Acylcarnitine hydrolase Hydrolase 96 Ces2e Pyrethroid hydrolase Hydrolase 96 EPHX1 Epoxide hydrolase 1 Hydrolase 127 Ephx2 Bifunctional epoxide hydrolase 2 Hydrolase 96 LDAH Lipid droplet-associated hydrolase Hydrolase 95, 127 CGI-58 Comparative gene identification-58 Lipid droplet metabolism 127 FAF2 FAS-associated factor 2 Lipid droplet metabolism 95, 127 Lipe Hormone-sensitive lipase Lipid droplet metabolism 96, 128 PLIN2 Perilipin 2/ADRP Lipid droplet metabolism 95, 127, 128, 129 PLIN3 Perilipin 3/TIP47 Lipid droplet metabolism 95, 96, 127, 129 PNPLA2 Patatin-like phospholipase domaincontaining protein 2 Lipid droplet metabolism 95, 127, 128 B4galnt2 Beta-1, 4 N-acetylgalactosaminyltransferase 2 Lipid glycosylation 96 Scp2 Non-specific lipid-transfer protein Lipid metabolism 96 Mogat2 Monoacylglycerol: acylcoa transferase 2 Lipid synthesis 128 Clint Clathrin interactor 1 Lipid transport 96 Dbi Diazepam binding inhibitor, Acyl-CoAbinding protein Lipid transport 96 Fabp1 Fatty acid-binding protein, liver Lipid transport 96 Fabp2 Fatty acid-binding protein, intestinal Lipid transport 96 ANXA2 Annexin A2 Lipoprotein metabolism 96, 127 Apoa1 Apolipoprotein A-I Lipoprotein metabolism 96

62 42 Table 1-1 Continued APOA4 Apolipoprotein A-IV Lipoprotein metabolism 96, 127 Apob Apolipoprotein B Lipoprotein metabolism 96 APOE Apolipoprotein E Lipoprotein metabolism 127 MGLL Monoglyceride lipase Lipoprotein metabolism 95, 127 MTTP Microsomal triglyceride transfer protein Lipoprotein metabolism 95, 96, 127, 128 P4HB Protein disulphide-isomerase Lipoprotein metabolism 95, 96, 127 ANXA4 Annexin A4 Membrane metabolism 96, 127 ELMOD2 ELMO domain-containing protein 2 Miscellaneous 127 DHRS1 Dehydrogenase/reductase SDR family member 1 Oxidation-reduction process 95, 96, 127 HSPA8 Heat-shock cognate 71 kda protein Phospholipid metabolism 96, 127 LPCAT2 Lysophosphatidylcholine acyltransferase 2 Phospholipid metabolism 95, 127 PCYT1A Choline-phosphate cytidylyltransferase A Phospholipid metabolism 127 Prdx6 Peroxiredoxin-6 Phospholipid metabolism 96 Atp5a1 ATP synthase subunit alpha, mitochondrial Regulation of lipid metabolism 96 Atp5b ATP synthase subunit beta, mitochondrial Regulation of lipid metabolism 96, 127 Cat Catalase Regulation of lipid metabolism 96 Golm1 Golgi membrane protein 1 Regulation of lipid metabolism 96 Adh1 Alcohol dehydrogenase 1 Retinal metabolism 96 Aldh1a1 Retinal dehydrogenase 1 Retinal metabolism 96 DHRS3 Short-chain dehydrogenase/reductase 3 Retinal metabolism 95, 127 Rbp2 Retinol-binding protein 2 Retinal metabolism 96 RDH10 Retinol dehydrogenase 10 Retinal metabolism 95, 127 SGPL1 Sphingosine-1-phosphate lyase 1 Sphingolipid metabolism 127 Cyb5a Cytochrome b5 Steroid metabolism 96 Cyp2b10 Cytochrome P450 2B10 Steroid metabolism 96 HSD17B11 Oestradiol 17- -dehydrogenase 11 Steroid metabolism 95, 96, 127 HSD17B2 Estradiol 17-beta-dehydrogenase 2 Steroid metabolism 95 HSD17B7 3-Oxo-steroid reductase Steroid metabolism 95, 127 HSD3B1 3- -Hydroxysteroid dehydrogenase Steroid metabolism 95, 127 Sult1b1 Sulfotransferase family cytosolic 1B member 1 Steroid metabolism 96 LSS Lanosterol synthase Sterol metabolism 95, 127 SQLE Squalene monooxygenase Sterol metabolism 95

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77 57 CHAPTER 2. CHARACTERIZATION OF THE PROTEOME OF CYTOPLASMIC LIPID DROPLETS IN MOUSE ENTEROCYTES AFTER A DIETARY FAT CHALLENGE 2.1 Abstract Dietary fat absorption by the small intestine is a multistep process that regulates the uptake and delivery of essential nutrients and energy. One step of this process is the temporary storage of dietary fat in cytoplasmic lipid droplets (CLDs). The storage and mobilization of dietary fat is thought to be regulated by proteins that associate with the CLD; however, mechanistic details of this process are currently unknown. In this study we analyzed the proteome of CLDs isolated from enterocytes harvested from the small intestine of mice following a dietary fat challenge. In this analysis we identified 181 proteins associated with the CLD fraction, of which 37 are associated with known lipid related metabolic pathways. We confirmed the localization of several of these proteins on or around the CLD through confocal and electron microscopy, including perilipin 3, apolipoprotein A-IV, and acyl-coa synthetase long-chain family member 5. The identification of the enterocyte CLD proteome provides new insight into potential regulators of CLD metabolism and the process of dietary fat absorption. This work was published in PLOSone T. D'Aquila, D. Sirohi, J.M. Grabowski, V.E. Hedrick, L.N. Paul, A.S. Greenberg, R.J. Kuhn, K.K. Buhman, Characterization of the proteome of cytoplasmic lipid droplets in mouse enterocytes after a dietary fat challenge, PloS one, 10 (2015) e

78 Introduction Dietary fat is the most energy dense macronutrient consumed and is required for the absorption of essential fatty acids and other lipophilic nutrients including fat soluble vitamins. However, when present in excess, dietary fat increases the risk for chronic diseases such as cardiovascular disease and obesity [1-4]. Therefore, understanding the regulators of dietary fat absorption and metabolism is important for both the promotion of health and prevention of disease. Dietary fat absorption by the small intestine is a multistep process. Triacylglycerol (TAG) is hydrolyzed by pancreatic lipase in the intestinal lumen producing monoacylglycerol and free fatty acids. These digestive products are taken up by the absorptive cells of the intestine, enterocytes, where they are rapidly resynthesized to TAG. The TAG is then packaged in the core of a chylomicron for systemic delivery of nutrients throughout the body [3,5,6]. Alternatively, when fatty acids are present in excess, the newly synthesized TAG may be incorporated into cytoplasmic lipid droplets (CLDs) within enterocytes. The size and number of CLDs within enterocytes increases and then decreases after consumption of dietary fat [7]; however, the factors that regulate CLD synthesis and catabolism within enterocytes are relatively unknown. The partitioning of TAG into chylomicrons or CLDs is important for determining the amount and rate of fatty acids delivered systemically. Therefore, identification of factors that regulate enterocyte CLD metabolism is important for understanding the overall process of dietary fat absorption.

79 59 Proteins that associate with CLDs in various cell types have been shown to regulate the synthesis and catabolism of CLDs [8-11]. Recently, our laboratory identified two CLD associated proteins, perilipin 2 (Plin2) and perilipin 3 (Plin3), on CLDs within enterocytes after a dietary fat challenge [12]. These well-established CLD associated proteins are thought to regulate lipolysis. In addition, mice deficient in mediators of lipolysis, including adipose triglyceride lipase [13] and abhydrolase domain containing 5 [14], have altered catabolism of CLDs in enterocytes. These results strongly suggest CLD associated proteins regulate the synthesis and catabolism of CLDs in enterocytes. The identification of additional CLD associated proteins within enterocytes has the potential to establish novel mediators of dietary fat absorption. The objective of this study was to identify CLD associated proteins in enterocytes after a dietary fat challenge. To fulfill this objective, we isolated CLDs from the small intestine of mice two hours after an oil bolus and identified proteins involved in the regulation of lipid trafficking and metabolism using a global proteomic approach. We further validated the presence of selected proteins on or around CLDs by confocal and immunoelectron microscopy.

80 Materials and methods Ethics statement We conducted the study in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institute of Health. The protocol was approved by the Purdue Animal Care and Use Committee (PACUC# ). All effort was made to minimize pain and suffering of mice and the number of mice used Mice C57BL/6 male mice from an in-house breeding colony were used for this study. The mice were maintained on a chow diet (PicoLab 5053, Lab Diets, Richmond, IN, USA) that consisted of 62.1% of calories from carbohydrate (starch), 24.7% from protein, and 13.2% from fat. The mice were housed in a temperature and humidity controlled facility with a 12 hour light/dark cycle (6AM/6PM) with ad libitum access to food and water Mouse procedure for lipid droplet isolation and proteomic analysis Four, 5 month old male C57BL/6 mice were fasted for 4 hours at the beginning of the light cycle. An oral gavage of 200 µl olive oil was administered and two hours after

81 61 the oil bolus, the mice were euthanized via CO2 asphyxiation. The small intestine was excised and divided into five equal length segments in relation to the stomach and labeled sections 1-5. Segments 2 and 3, representing the jejunum, were used for analysis Enterocyte Isolation Enterocytes were isolated from sections 2 and 3 of the small intestine as previously described [12,15]. Briefly, the intestinal sections were washed in tissue buffer (Hank's Balanced Salt Solution with 25 mm HEPES and 1% fetal calf serum) and then placed in isolation buffer (Calcium and magnesium free Hank's Balanced Salt Solution with 1.5mM EDTA). The intestine segments were incubated for 15 minutes at 37 C with rotation. The sample was mixed briefly (vortexed) and the supernatant containing enterocytes was removed and saved. The process was repeated and the supernatants containing isolated enterocytes were combined CLD isolation CLDs were isolated from enterocytes using a previously established sucrose gradient ultracentrifugation protocol [16]. Enterocytes were lysed in ice cold sucrose lysis buffer (175mM sucrose, 10 mm HEPES and 1 mm EDTA ph 7.4). Cells were disrupted by passing through a 27 gauge, 1 inch needle, eight times. The resulting two mls of cell lysate were carefully layered with six mls of sucrose-free lysis buffer and centrifuged at

82 62 20,000 x g at 4 C for two hours. After centrifugation, the sample was frozen at -80 C. The frozen sample was sliced into seven sequential fractions which were approximately 1 cm in length TAG and protein analysis TAG concentration of each fraction was determined using an L Type TG M assay (Wako Diagnostics, Richmond VA, USA). Protein concentration of each fraction was determined by a BCA assay (Thermo Scientific, Rockford IL, USA). Additionally, a Western Blot analysis was performed on each fraction. Briefly, samples from the isolated fractions were delipidated using 10% sodium dodecyl sulfate, separated by polyacrylamide gel electrophoresis, and transferred to a PVDF membrane. The membranes were probed overnight at 4 C with antibodies for Plin3 (a gift from Dr. Perry Bickel at the University of Texas Southwestern, Dallas TX, USA), glyceraldehyde 3- phosphate dehydrogenase (Gapdh) (Abcam, Cambridge MA, USA), or calnexin (Cnx) (Santa Cruz Biotechnology, Dallas TX, USA). These proteins were used as markers for the CLD, cytosolic, or membrane fractions, respectively. The blots were then probed with a LI-COR IR DYE 800CW secondary antibody and imaged using Odyssey CLx Infrared imaging system (LI-COR Biosciences, Lincoln NE, USA) In solution digestion and LC MS/MS In preparation for proteomic analysis, the isolated CLD fractions were delipidated using 2:1 chloroform methanol and proteins precipitated using ice cold acetone. The

83 63 protein pellet was denatured using 8M urea and 10 mm DTT for 1.5 hours at 37 C. The sample was digested for 12 hours using trypsin (Sigma-Aldrich, St. Louis MO, USA) using a ratio of 1 µg trypsin to 50 µg protein. The reaction was quenched using trifluoroacetic acid. Tryptic peptides were separated on a nanolc system (1100 Series LC, Agilent Technologies, Santa Clara, CA). The peptides were loaded on the Agilent 300SB-C18 enrichment column for concentration and the enrichment column was switched into the nano-flow path after five minutes. Peptides were separated with a C18 reversed phase ZORBAX 300SB-C18 column. The column was connected to the emission tip and coupled to the nano-electrospray ionization (ESI) source of the high resolution hybrid ion trap mass spectrometer LTQ-Orbitrap LX (Thermo Fisher Scientific, Waltham MA, USA). The LTQ-orbitrap mass spectrometer was operated in the datadependent positive acquisition mode in which each full MS scan ( resolving power) was followed by six MS/MS scans where the six most abundant molecular ions were selected and fragmented by collision induced dissociation (CID) using a normalized collision energy of 35% Data analysis and bioinformatics The peak list files containing MS and MS/MS data were analyzed using MaxQuant version [17]. For protein identification, the MS/MS data was searched against the Uniprot protein database which includes the Swissprot (manually annotated and reviewed) and TrEMBL (automatically annotated and not reviewed) databases [18]. The database was searched using the MASCOT search engine utilizing Andromeda as the

84 64 peptide search algorithm that is incorporated in the MaxQuant platform [19]. The search was conducted using the following settings: trypsin cleavage with a maximum of two missed cleavages, fixed modification of iodoethanol addition to cysteine, variable modification of oxidation of methionine. The MS mass tolerance was set at 4.5 ppm with a maximum number of five modifications. The false discovery rate was set at 0.01 for proteins and peptides and was run against a decoy revert database. Peptides required a minimum length of seven amino acids. The MS/MS tolerance was set at 0.1 Da for protein identification. The minimum score for modified and unmodified peptides was set at forty. At least two peptides were required for protein identification. The bioinformatics and statistical package Perseus was used to analyze the MaxQuant output. Contaminants identified by Perseus, such as keratin, were removed from the analysis. For label free quantitation (LFQ), the intensity for each protein was transformed log2(x) and samples who had a peptide intensity below the level of detection were assigned a value of 14. We limited the analysis to proteins which were identified in at least 3 out of the 4 biological replicates. Proteins identified were clustered by their Gene Ontology (GO) Term based on biological process or molecular function using the Database for Annotation, Visualization and Integrated Discovery (DAVID) v 6.7 [20,21]. Visualization of protein interactions was accomplished using STRING version 9.1[22]. We used the confidence view with a score of 0.4, indicating a medium confidence level. We did not limit the number of interactions in the analysis.

85 Immunofluorescence Imaging Four, C57BL/6 male mice were fasted for 4 hours at the beginning of the light cycle and then administered a 200 µl olive oil bolus. A small (5mm) section of the jejunum was harvested from the mice two hours after the dietary fat challenge and was frozen in optimal cutting temperature embedding media. The tissue was stored at -80 C until processed by immunofluorescence microscopy. The tissue was sliced into 10 µm tissue sections, fixed in 2% paraformaldehyde, and permeabilized with 0.1% saponin. The tissue was probed with previously validated antibodies for Plin3 [23] (a gift from Dr. Perry Bickel at the University of Texas Southwestern, Dallas TX, USA), apolipoprotein A-IV (ApoA-IV) [24] (a gift from Dr. Patrick Tso from the University of Cincinnati, Reading OH, USA), and acyl-coa synthetase long-chain family member 5 (Acsl5) (personal communication with A. Greenberg) (a gift from Dr. Andrew Greenberg from Tufts University, Boston MA, USA). The sections were also stained for neutral lipids -difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY) (Life technologies, Grand Island NY, USA), a secondary AlexaFluor antibody (Life technologies), and for nuclei using 300 nm DAPI (Life technologies). The sections were imaged using a Nikon A1R confocal microscope (Nikon Instruments Inc., Melville NY, USA). Image processing, co-localization analysis, and multidimensional imaging were conducted using NIS- Elements C acquisition and analysis software.

86 Transmission electron microscopy Cardiac perfusion fixation and TEM imaging of jejunum Two, C57BL/6 mice were fasted for 4 hours at the beginning of the light cycle. An oral gavage of 200 µl olive oil was administered and two hours after the oil bolus, the mice were anesthetized using inhaled isoflurane and perfused with 1.5% glutaraldehyde in 0.1M sodium cacodylate via cardiac infusion. After fixation, a sample of the jejunum section of the small intestine was isolated, stained with osmium tetroxide, dehydrated, and embedded in resin. Ultrathin sections were stained with lead citrate and uranyl acetate and examined using a Tecnai T20 Transmission Electron Microscope (FEI, Hillsboro OR, USA) TEM imaging of isolated CLDs A sample from the isolated CLD fraction was negatively stained using 2% w/v aqueous uranyl acetate. The isolated CLDs were imaged using a Tecnai T20 Transmission Electron Microscope (FEI) Immunoelectron microscopy Two, C57BL/6 mice were fasted for 4 hours at the beginning of the light cycle. An oral gavage of 200 µl olive oil was administered and two hours after the oil bolus, the mice were anesthetized using inhaled isoflurane and perfused with 4% paraformaldehyde and 0.05% glutaraldehyde in 0.1M phosphate buffer via cardiac infusion. After fixation, a

87 67 sample of the jejunum section of the small intestine was isolated, dehydrated, and embedded in resin. Ultrathin sections were probed with an antibody for Acsl5 (a gift from Dr. Andy Greenberg), gold conjugated secondary antibody (Ted Pella Inc. Redding, CA), and stained with uranyl acetate. The samples were examined using Tecnai T20 Transmission Electron Microscope (FEI). 2.4 Results Lipid accumulates in CLDs in enterocytes in response to a dietary fat challenge. Neutral lipids accumulate in distinct regions of the enterocyte following an acute olive oil bolus. Enterocytes from the jejunum section of the small intestine, two hours after a dietary fat challenge, were stained with osmium tetroxide and examined by transmission electron microscopy (Figure 1). The various fates of neutral lipids within enterocytes following a dietary fat challenge are identified in this image. Neutral lipids accumulate in large CLDs which are indicated by white asterisks. Also visible is the Golgi apparatus (white arrows) which contains smaller chylomicron sized particles. Additionally, the white areas between enterocytes contain secreted chylomicrons (black cross). These results highlight the complex, multistep process of dietary fat absorption The CLD fraction is enriched with Plin3 and has a high TAG to protein ratio. Enterocyte CLDs were isolated via density gradient ultracentrifugation using a previously established protocol [16]. After centrifugation, the samples were frozen and

88 68 sliced into seven fractions, where fraction 1 contains CLDs (Figure 2a). To confirm the successful isolation of CLDs, the isolated fractions were subjected to Western blot analysis. Antibodies for Plin3, Gapdh, and Cnx, were used as markers of the CLD, cytosolic, and membrane fractions respectively. The CLD marker Plin3 was found in fractions 1, 4, 5, 6, and 7 (Figure 2c), representing the various fractions Plin3 has been previously described to locate [25]. Plin3 is found primarily in the cytosol when lipids are absent and translocate to the nascent CLD in the early stages of CLD formation. Gapdh was used as a cytosolic marker and was found primarily in the cytosolic fractions (fractions 2-6). In addition, Gapdh was notably absent in fraction 1. Cnx was used as a marker for membranes and found primarily in fraction 7. To validate the presence of CLDs, the TAG to protein ratio of each fraction was examined (Figure 2b). Fraction 1 contained a high concentration of TAG compared to protein, indicating the presence of CLDs. Fraction 1 was also examined using negative staining electron microscopy which showed an abundance of round, CLD sized particles (Figure 2d). This data suggests that CLDs were successfully isolated from mouse enterocytes Proteins were identified in the CLD fraction through LC MS/MS To identify proteins that associate with the CLD fraction, we utilized a shotgun proteomic approach. Through high resolution tandem mass spectroscopy, we identified 181 proteins in at least 3 out of the 4 biological replicates (Data not shown). Proteins were identified by comparing the MS/MS spectra against the Uniprot database. The Uniprot database combines FASTA files from the Swissprot and TrEMBL databases.

89 69 Using Maxquant s LFQ [26], the relative abundance of each protein within each sample was identified. It should be noted that the LFQ intensity is the result of multiple factors, including the abundance of the protein within the sample and the ability of the peptides to be ionized and detected. To identify the function of the proteins, we used the functional annotation tool in DAVID. The Gene Ontology (GO) terms of molecular function and biological process associated with each protein was identified and then GO terms were clustered into broader classifications as described in Table 1. The percentage of proteins categorized within broader classifications is found in Figure 3. On several occasions, the GO terms associated with a protein would fall under multiple broader classifications. Under these circumstances, a manual analysis of the protein s function was conducted using the Uniprot and NCBI databases. Based on the quantity and quality of reported protein function, the protein was assigned into the most appropriate GO term and broader classification Some identified proteins in the CLD fraction are associated with known lipid metabolism related functions Using DAVID s functional analysis tool, 19% of the proteins identified in the CLD fraction were found to be associated with a biological process or molecular function related to lipid metabolism. The relative abundance of these proteins was determined by Maxquant s LFQ (Table 2). Approximately half of these proteins have been identified in other CLD proteomic studies from diverse cell types (Table 2). We specifically highlighted proteins which have been previously identified associated with CLDs in an

90 70 intestinal cell model through proteomic analysis. A map of predicted interactions was generated using a STRING analysis (Figure 4) [22]. The resulting network shows several protein clusters including proteins responsible for lipoprotein synthesis and modifiers of fatty acids Immunofluorescence imaging of proteins identified in the CLD fraction supports their CLD localization To confirm the localization of ApoA-IV and Acsl5 on or around CLDs, we probed intestinal sections with antibodies for these proteins from four, male C57BL/6 mice, two hours after a 200 µl olive oil bolus. To visualize CLDs, we stained neutral lipids with BODIPY (Life technologies) and used immunofluorescence staining of a well-established marker of enterocyte CLDs, Plin3 [12]. Due to the process of fixation, neutral lipids are often depleted from CLDs; therefore, where applicable, both Plin3 and neutral lipid staining was used to enhance our ability to detect CLDs. The first protein investigated was ApoA-IV, a protein known regulate intestinal lipid absorption and chylomicron synthesis [27,28]. To confirm the localization of ApoA-IV to the area on or around the CLD, the signals from Plin3 (Figure 5A) and ApoA-IV (Figure 5B) were merged and overlaid with signals from nuclei (blue) and neutral lipid (orange) (Figure 5C). A region of interest was highlighted (white capped line) in Figure 5C. The intensity profile of all fluorescent signals along this line was generated (Figure 5D). The intensity signals from Plin3 and ApoA-IV overlap and form two peaks which flank the signal from neutral lipids. Additionally, the signal from ApoA-IV and Plin3 antibodies colocalize with a Pearson s coefficient of r= 0.59 and a Mander s overlap of r= 0.96 which indicates

91 71 a high degree of correlation. This indicates that ApoA-IV localizes to the area on or around the CLD. A three dimensional volume view was generated from Z-series images (Figure 5E) and illustrates the localization of Plin3 and ApoA-IV to the area on or around the CLD forming a protein coat. The intensity profile, colocalization analysis, and volume view was generated using NIS- Elements C software. The proteomic analysis of isolated CLDs also identified Acsl5, an enzyme that activates fatty acids by the addition of coenzyme A [29]. In this experiment, we were unable to use Plin3 as a marker for CLD due to the fact the antibodies for Acsl5 and Plin3 were generated in the same host species, so CLDs are identified using only BODIPY. Intestinal sections were probed with an antibody for Acsl5 (green) (Figure 6a), stained for neutral lipids using BODIPY (orange) and nuclei using DAPI (blue) (Figure 6B). Acsl5 forms distinct ring like structures on or around CLDs as indicated by the white arrows. A 3D volume view was generated from Z series images (Figure 6C) showing the localization of Acsl5 to the area on or around CLDs. Additionally, to confirm the localization of Acsl5 to CLDs in enterocytes following a 200 µl olive oil bolus, we performed immunogold labeling of ultra-thin sections of the jejunum section of the small intestine. An electromicrograph (Figure 6D) shows an enterocyte with large white CLDs present. Areas of interest are highlighted by colored arrows which correspond with figures 6 E-H. Acsl5 conjugated gold particles are found on the CLD surface which are indicated by the white arrows (Figure 6E-G). In figure 6E, Acsl5 can be seen localized to the CLD as opposed to the endoplasmic reticulum membrane which can been seen on the

92 72 left hand side of the CLD. Additionally, Acsl5 can be found localized to the mitochondria (Figure 6H) which has been previously reported in Caco-2 cells[30,31] and liver McArdle-RH7777 cells[32]. 2.5 Discussion To increase our understanding of the process of dietary fat absorption, we investigated a key step of this process, the storage of dietary fat in CLDs. Proteins associated with CLDs are likely involved in both the storage and trafficking of fatty acids as well as other cellular processes. Therefore, we analyzed the proteome of CLDs from enterocytes isolated from the jejunum section of the small intestine following an oil bolus using high resolution tandem mass spectroscopy. We identified 181 proteins associated with the CLD fraction (Data not shown) that are associated with a wide range of biological and molecular functions (Figure 3). Several of the identified proteins associated with lipid metabolism have been previously identified in other CLDs proteomic analyses from many cell types, while other identified proteins have not been previously reported (Table 2). Furthermore, we confirmed the localization of select proteins through immunohistochemistry and immunoelectron microscopy (Figures 5 and 6). Of the 181 proteins identified in the CLD fraction of mouse enterocytes, thirty seven proteins are associated with known lipid related processes. Within this subgroup, twenty three proteins (62%) have been identified in CLD proteomic analyses in various cell types and fourteen proteins (38%) have not been previously reported in CLD proteomic analyses from any cell type (Table 2). Interestingly, three of the CLD proteins,

93 73 ApoA-IV, Acsl5, and microsomal triglyceride transfer protein (Mttp), have been previously identified in a human cell model of enterocytes, Caco-2 cells, but not other cell types [33,34]. Therefore, these proteins may be unique to the CLD of enterocytes and serve a function on CLDs in dietary fat absorption not previously recognized. We confirmed the localization of ApoA-IV and Acsl5 on or around CLDs through immunohistochemistry and immunoelectron microscopy analysis of mouse enterocytes after a high fat challenge. ApoA-IV may play important roles in dietary fat absorption due to its localization on lipoproteins and CLDs [33,35]. Both lipoproteins and CLDs function as lipid storage vesicles and are surrounded by a phospholipid monolayer and a diverse array of proteins [3,36]. Many of these proteins regulate synthesis and catabolism of these similar structures. Therefore, the identification of common proteins on lipoproteins and CLDs is not surprising. We observed ApoA-IV forming a distinct ring like structure around CLDs and co-localizing with Plin3, an established CLD protein, through immunofluorescence imaging. Consistent with this result, ApoA-IV was also identified on CLDs in Caco-2 cells via immunoelectron microscopy [33]. Although the precise function of ApoA-IV on lipoproteins is not clear, expression of ApoA-IV regulates lipoprotein synthesis and satiety. When over-expressed, ApoA-IV increases TAG secretion from the liver and intestine, by increasing the amount of lipid packaged on apolipoprotein B (ApoB) containing lipoprotein particles [37-40]. Furthermore, ApoA- IV regulates feeding behavior by trafficking to the brain and serving as a satiety signal [41]. Current knowledge of ApoA-IV s role in lipoprotein metabolism can be applied to the formation of hypotheses regarding the role of ApoA-IV on CLDs. ApoA-IV may regulate CLD

94 74 synthesis and/or regulate satiety through sequestration of the protein on the CLD. These hypotheses remain to be tested. Although the identification of lipoprotein associated proteins in the CLD fraction is not surprising, it is important to consider the potential for contamination of the CLD fraction by lipoproteins. To minimize contamination of the CLD fraction by smaller, lipid containing particles the samples were centrifuged at a relatively low speed (20,000 x g). Smaller lipid containing particles such as lumenal lipid droplets, pre-chylomicron transport vesicles, and chylomicrons, are isolated at much faster centrifugation speeds, 106,000 x g [42], 100,000 x g [43], and 197,000 x g [44], respectively. Despite efforts to minimize lipoprotein contamination, we still identified several proteins which are established for association with lipoproteins including ApoA-IV, ApoB, and Mttp. This observation is consistent with other CLD proteomic analyses from a range of cell types that have also identified lipoprotein associated proteins [33,45], including cell types not known to produce lipoproteins [46,47]. Furthermore, ApoA-IV and ApoB were demonstrated to localize to CLDs through immunoelectron microscopy in Caco-2 cells [33] and hepatocytes [48] respectively. Together, these results support that a protein may be associated with both lipoproteins and CLDs. Acsl5 may play a role in localized TAG synthesis on CLDs in enterocytes as part of the process of dietary fat absorption. It was recently demonstrated in lipid challenged Drosophilia S2 cells that TAGs synthesis enzymes localize to CLDs including glycerol-3- phosphate acyltransferase 4, 1-acyl-sn-glycerol-3-phsophate acyltransferase gamma, and diacylglycerol acyltransferase 2 [11]. In the intestine, two TAG synthesis enzymes that catalyze the committed step in TAG biosynthesis, diacylglycerol acyltransferase 1 and

95 75 diacylglycerol acyltransferase 2 were identified as associated with CLDs by western blotting [49]. Additionally, Acsl enzymes activate fatty acids and channel them towards specific metabolic fates within cells [50]. Interestingly, other Acsl family members including Acsl1 [45,46], Acsl3 [33,34,46], and Acsl4 [33,46] have been identified on CLDs in various cell types. In particular, Acsl3 over-expression in Cos-1 cells results in generation and expansion of CLDs in response to fatty acids [51]. Finally, Acsl5 directs exogenous fatty acids toward TAG synthesis in hepatocytes [32,52]. Therefore, one hypothesis for the function of Acsl5 on CLDs in enterocytes is the activation of fatty acids for TAG synthesis; however, this hypothesis remains to be tested. A limitation to a proteomic approach for identifying CLD associated proteins is the challenge to distinguish protein contamination from other cellular organelles from bona fide CLD proteins in the isolated CLD fraction. It is possible that proteins from other organelles associate with the CLD fraction nonspecifically during cell lysis because of the normal tight association of CLDs with other organelles [53]. Methods described in the literature for isolation of CLDs vary in how cells are lysed and the type and numbers of washes used. Currently there is not one commonly accepted method for CLD isolation to alleviate this problem. CLD isolation protocols involving multiple washing steps remove proteins known to associate with other organelles, as well as decrease the levels of well-established CLD proteins [45]. In the current study, we used a single step isolation protocol to preserve potentially loosely bound CLD proteins and reduce the risk of missing key players in CLD metabolism. However, we only included proteins identified in 3 of 4 biological replicates to rule out random contamination. Due to these limitations, each protein identified needs to be validated by other methods such as

96 76 imaging to confirm the localization to CLDs in enterocytes before making firm conclusions. Despite these limitations, the identification of Acsl5 on CLDs, ER, and mitochondria highlights that a single protein may have multiple associations in the cell. The protein list generated here is intended to be used as an informed, hypothesis generating tool to prioritize future research, not as a list of established proteins on CLDs in enterocytes. The proteins identified in the current study build upon an existing body literature of CLD biology, from which interesting commonalities are being observed. A key feature, of all CLD proteomic analyses from multiple cell types, is the identification of non-lipid related proteins in the CLD fraction. These proteins fall into a wide range of biological and molecular functions such as amino acid metabolism, carbohydrate metabolism, and membrane trafficking (Figure 3) [33,34,45-47,54-58]. This consistent observation has not been fully investigated and remains an intriguing paradox of CLD biology. Several hypotheses have been proposed to address this issue including the role of CLDs as sequestration sites [59,60] or in the regulation of membrane trafficking [61]. The identification of non-lipid related protein in the CLD fraction has expanded our perception of the CLD from a simple lipid storage site to a complex and multi-functional organelle [62]. We successfully isolated CLDs from the jejunum of mice after a dietary fat challenge and identified the proteome of the CLDs. Similar to previously reported CLD proteomic analyses, the proteins identified in the CLD fraction are associated with a wide range of biological processes and molecular functions. Of interest, ApoA-IV and Acsl5 were identified in the proteomic analysis of the CLD fraction and the presence was

97 77 confirmed through immunofluorescence and immunoelectron microscopy. The role of many of these lipid related proteins on the CLD is currently unknown, however the proteomic analysis provides a foundation to further investigate the role of these proteins and advance the model of dietary fat absorption and lipid trafficking in the small intestine. 2.6 Acknowledgements We would like to acknowledge the help and support of Yu-Han Hung and the Bindley Bioscience Center, in particular Ernesto S. Nakayasu, Aaron Taylor, and members of the multi-scale imaging center for their help and expertise. This project was supported by the Indiana Clinical and Translational Sciences Institute, by Grant # UL1TR from the National Institutes of Health, National Center for Advancing Translational Sciences, Clinical and Translational Sciences Award (KKB), Grant # 7-13-IN-05 by the American Diabetes Association, Innovation Award (KKB), Grant # R01 DK from the National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) (KKB, ASG), Grant # P30 DK National Institutes of Health, NIDDK (ASG), Grant # U01 ES National Institutes of Health, National Institute of Environmental Health Science (ASG), and the Purdue Research Foundation, Graduate Fellowship Award (KKB, TD). This material is based upon work supported by the U.S. Department of Agriculture, under agreement No (ASG).

98 78 Table 2-1 GO Terms associated with protein classification term. GO terms of biological process and molecular function were grouped under broader classifications. Proteins were assigned into a broader classifications based on the associated GO terms. Classification GO_id GO Term Number of proteins Translation GO: Translation 22 GO: regulation of translation 5 Carbohydrate GO: cellular carbohydrate catabolic process 8 GO: carbohydrate catabolic process 11 GO: cellular carbohydrate metabolic process 11 GO: carbohydrate binding 9 GO: carbohydrate biosynthetic process 6 Cytoskeletal GO: actin filament-based process 12 GO: actin filament binding 5 GO: cytoskeletal protein binding 11 Lipid GO: lipid metabolic process 21 GO: cellular lipid metabolic process 18 GO: lipid binding 17 GO: regulation of lipid metabolic process 10 GO: lipid catabolic process 7 GO: lipid particle 6 GO: hydrolase activity, acting on ester bonds 10 Protein localization and transport Protein folding and metabolism RNA and transcription GO: protein localization 18 GO: regulation of protein localization 6 GO: protein transport 15 GO: protein metabolic process 47 GO: protein complex subunit organization 23 GO: protein homodimerization activity 19 GO: protein complex assembly 18 GO: protein complex binding 17 GO: regulation of protein metabolic process 16 GO: ribonucleoprotein complex biogenesis 12 GO: regulation of RNA metabolic process 17 GO: RNA localization 4 GO: regulation of RNA biosynthetic process 15 GO: RNA processing 14 Redox GO: oxidation-reduction process 27 GO: cell redox homeostasis 4 GO: peroxiredoxin activity 3

99 79 Table 2-2 A sub group of proteins are associated with known lipid related functions. Thirty seven proteins associated with known lipid metabolism pathways were identified, of which twenty three proteins have been previously identified in other CLD proteomic analyses. Relative levels of the proteins were determined by LFQ and the average is reported (n=4 mice). Avg LFQ Protein name Gene name Uniprot IDs Identified in other CLD studies Long-chain-fatty-acid--CoA ligase 5 Acsl5 Q8JZR0 [34] Alcohol dehydrogenase 1 Adh1 Q3UKA4 [45,54,55,58] Retinal dehydrogenase 1 Aldh1a1 P24549 [45] Annexin A2 Anxa2 P07356 [46,47,55,58, 63] [33] Annexin A4 Anxa4 Q7TMN7 [47] [33] Apolipoprotein A-I Apoa1 Q3V2G1 [45-47] Apolipoprotein A-IV Apoa4 Q9DBN0 [33] Apolipoprotein B Apob E9Q ATP synthase subunit alpha, mitochondrial ATP synthase subunit beta, mitochondrial Beta-1,4 N- acetylgalactosaminyltransferase 2 Atp5a1 Q03265 [45,47] Atp5b P56480 [45,47] B4galnt2 Q (2),5-bisphosphate nucleotidase 1 Bpnt1 Q9Z0S Catalase Cat Q8C6E3 [45,64] Carboxylesterase 2a Ces2a Q8QZR Carboxylesterase 2c Ces2c Q91WG Carboxylesterase 2e Ces2e Q8BK Clathrin interactor 1 Clint1 Q5SUH Cytochrome b5 Cyb5a P56395 [45,47] Identified in intestine model

100 80 Table 2-2: Continued NADH-cytochrome b5 reductase 3 Cyb5r3 Q9DCN2 [45] [33,34] Cytochrome P450 2B10 Cyp2b10 Q9WUD Acyl-CoA-binding protein Dbi Q548W7 [47] Bifunctional epoxide hydrolase 2 Ephx2 Q3UQ71 [45] Fatty acid-binding protein, liver Fabp1 Q3V2F7 [45] Fatty acid-binding protein, intestinal Fabp2 Q53YP Golgi membrane protein 1 Golm1 Q91XA Hydroxyacyl-coenzyme A dehydrogenase Hadh Q61425 [46] Estradiol 17-beta-dehydrogenase 11 Hsd17b11 Q9EQ06 [45,46] [33,34] Hormone-sensitive lipase Lipe P54310 [46,47,56,57] [33,34] Microsomal triglyceride transfer protein Mttp O08601 [33,34] Nucleoside diphosphate kinase Nme2 Q Perilipin-3 Plin3 Q9DBG5 [46,56,58 [33,34],64-66] Peroxiredoxin-6 Prdx6 Q6GT24 [45] Retinol-binding protein 2 Rbp2 Q Non-specific lipid-transfer protein Scp2 P32020 [45,46] Sulfotransferase family cytosolic 1B member 1 Sult1b1 Q9QWG UDP-glucuronosyltransferase 1-7C Ugt1a7c Q6ZQM Transitional endoplasmic reticulum ATPase Vcp Q8BNF8 [45-47]

101

102

103

104

105

106

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112 92 CHAPTER 3. DIET INDUCED OBESITY ALTERS CYTOPLASMIC LIPID DROPLET MORPHOLOGY AND ASSOCIATED PROTEINS IN RESPONSE TO AN ACUTE DIETARY FAT CHALLENGE 3.1 Abstract Dietary fat absorption by the small intestine is an efficient, multistep process that regulates the uptake and delivery of essential nutrients and energy. Fatty acids taken up by enterocytes, the absorptive cells of the small intestine, are resynthesized into triacylglycerol (TAG) and either secreted in chylomicrons or temporarily stored in cytoplasmic lipid droplets (CLDs). Proteins that associate with CLDs are thought to regulate the dynamics of TAG storage and mobilization. It is currently unclear what affect diet induced obesity (DIO) has on the balance between dietary fat storage and secretion. Specifically, there is limited knowledge of how DIO affects the level and diversity of proteins that associate with CLDs and regulate CLD dynamics. In the current study, we characterize CLDs from lean and DIO mice through histological and proteomic analyses. We demonstrate that DIO mice have larger intestinal CLDs compared to lean mice in response to dietary fat. Additionally we identified 375 proteins associated with CLDs in lean and DIO mice. We identify a subgroup of lipid related proteins which are either increased or unique to the DIO CLD proteome. These proteins are involved in

113 93 steroid synthesis pathways, TAG synthesis, and lipolysis. This analysis expands upon our knowledge of the effect of DIO obesity on the process of dietary fat absorption in the small intestine. 3.2 Introduction Enterocytes, the absorptive cells of the small intestine, are responsible for the uptake, repackaging, and secretion of dietary fat. In addition, enterocytes are capable of temporarily storing dietary fat in CLDs in response to a dietary fat challenge (Reviewed in [1, 2]) Dietary fat in the form of TAG is digested in the lumen of the small intestine producing FFA and MAG which are incorporated into mixed micelles. Mixed micelles interact with the brush border membrane of enterocytes and FFA and MAG are taken up by enterocytes. In the enterocyte FFA and MAG are resynthesized into TAG by MGAT and DGAT at the ER. The resulting TAG can be packaged onto a chylomicron particle for secretion or temporarily stored in CLDs. CLDs are composed of a neutral lipid core, a phospholipid monolayer, and associated proteins [3]. Proteins which associate with CLDs are thought to regulate the storage and mobilization of TAG. Proteins associated with CLDs isolated from enterocytes after a lipid challenge have been identified by targeted [4] and global proteomic [5-7] approaches. These studies have identified and confirmed members of the perilipin family (Plin2 and Plin3) associated with CLDs. Interestingly, these studies have also identified proteins associated with a wide variety of biological pathways including lipid catabolic and anabolic pathways.

114 94 Obesity alters the process of dietary fat absorption. The effect of obesity on intestinal lipid metabolism is currently unclear, but may be dysregulated by obesity and associated diseases (recently reviewed [8]). Recent studies in high fat diet fed (non obese), ob/ob mice, and DIO mice have demonstrated decreased TAG secretion rates in response to an acute dietary fat challenge, when lipoprotein lipase has been inhibited with tyloxapol [9, 10]. Inhibition of lipoprotein lipase blocks the clearance of lipoproteins resulting in a dramatic increase in blood TAG levels. DIO alters intestinal mrna levels of proteins involved in chylomicron assembly, TAG synthesis, and fatty acid trafficking [10]. Little is known on the effect of obesity on CLD morphology and associated proteins in enterocytes. DIO alters CLD associated proteins in adipocytes and hepatocytes [11, 12].Little is known on the effect of obesity on the CLD proteome in enterocytes. Previous studies have shown alterations in mrna levels of proteins known to associate with CLDs including lipases [10] and members of the perilipin family[13]. Plin2 and 3 localize to CLDs in response to a dietary fat challenge, however under different physiological conditions. Plin3 localizes to CLDs in response to an acute dietary Plin2 localizes to CLDs in response to chronic high fat feeding in a DIO mouse model [13]. A comprehensive analysis of proteins associated with CLDs from lean and DIO mice in response to an acute dietary fat challenge has not been conducted ` To determine the effects of DIO on enterocyte CLD morphology and associated proteins in response to consumption of an acute dietary fat challenge, we investigated CLDs within enterocytes of lean and DIO mice given an oil bolus orally by transmission

115 95 electron microscopy, immunofluorescence microscopy, and proteomic analysis. Based on previous observations [9, 10, 13] of altered TAG secretion in DIO mice in response to a dietary fat challenge, we hypothesized that DIO mice have altered CLD morphology and CLD associated proteins. 3.3 Materials and methods Mice C57BL/6 male mice from an in-house breeding colony were used for this study. The mice were maintained on a chow diet (PicoLab 5053, Lab Diets, Richmond, IN, USA) that consisted of 62.1% of calories from carbohydrate (starch), 24.7% from protein, and 13.2% from fat from weaning to 5 weeks of age. The mice were housed in a temperature and humidity controlled facility with a 12 hour light/dark cycle (6AM/6PM) with ad libitum access to food and water Generation of DIO mouse model C57BL/6 male mice were placed on a either high fat diet or low fat diet. The high fat diet (D12492, Research Diet, New Brunswick, NJ, USA) consists of 20% of calories from carbohydrates, 20% from protein, and 60% from fat. The low fat, D12492 matched diet (D12450J, Research Diet, New Brunswick, NJ, USA) consists of 70% of calories from carbohydrates, 20% from protein, and 10% from fat. The mice were maintained on the diet for 12 weeks and body weights were monitored weekly.

116 Mouse procedure for lipid droplet isolation and proteomics analysis Five mice from the low fat diet group and 5 mice from the high fat diet group were oil was administered and two hours after the oil bolus, the mice were euthanized via CO2 asphyxiation. The small intestine was excised and divided into three equal length segments in relation to the stomach. The middle segment, representing the jejunum, was used for the analysis Enterocyte isolation Enterocytes were isolated from the middle section of the small intestine as previously described [13, 14]. Briefly, the intestinal sections were washed in tissue buffer (Hank's Balanced Salt Solution with 25 mm HEPES and 1% fetal calf serum) and then placed in isolation buffer (Calcium and magnesium free Hank's Balanced Salt Solution with 1.5mM EDTA). The intestine segments were incubated for 15 minutes at 37 C with rotation. The sample was mixed briefly (vortexed) and the supernatant containing enterocytes was removed and saved. The process was repeated and the supernatants containing isolated enterocytes were combined CLD isolation CLDs were isolated from enterocytes using a previously established sucrose gradient ultracentrifugation protocol [7]. Enterocytes were lysed in ice cold sucrose lysis

117 97 buffer (175mM sucrose, 10 mm HEPES and 1 mm EDTA ph 7.4). Cells were disrupted by passing through a 27 gauge, 1 inch needle, eight times. The resulting two mls of cell lysate were carefully layered with six mls of sucrose-free lysis buffer and centrifuged at 20,000 x g at 4 C for two hours. After centrifugation, the sample was frozen at -80 C. The frozen sample was sliced into seven sequential fractions which were approximately 1 cm in length In solution digestion and LC MS/MS In preparation for proteomic analysis, the isolated CLD fractions were delipidated using 2:1 chloroform methanol and proteins precipitated using ice cold acetone. The protein pellet was denatured using 8M urea and 10 mm DTT for 1.5 hours at 37 C. The sample was digested for 12 hours using trypsin (Sigma-Aldrich, St. Louis MO, USA) trifluoroacetic acid. Tryptic peptides were separated on a nanolc system (1100 Series LC, Agilent Technologies, Santa Clara, CA). The peptides were loaded on the Agilent 300SB-C18 enrichment column for concentration and the enrichment column was switched into the nano-flow path after five minutes. Peptides were separated with a C18 reversed phase ZORBAX 300SB-C18 column. The column was connected to the emission tip and coupled to the nano-electrospray ionization source of the high resolution hybrid ion trap mass spectrometer LTQ-Orbitrap LX (Thermo Fisher Scientific, Waltham

118 98 MA, USA). The LTQ-orbitrap mass spectrometer was operated in the data-dependent positive acquisition mode in which each full MS scan ( resolving power) was followed by six MS/MS scans where the six most abundant molecular ions were selected and fragmented by collision induced dissociation (CID) using a normalized collision energy of 35% Protein identification The peak list files containing MS and MS/MS data were analyzed using MaxQuant version [15, 16]. For protein identification, the MS/MS data was searched against the Uniprot protein database[17]. The database was searched using the MASCOT search engine utilizing Andromeda as the peptide search algorithm that is incorporated in the MaxQuant platform [18]. The search was conducted using the following settings: trypsin cleavage with a maximum of two missed cleavages, fixed modification of iodoethanol addition to cysteine, variable modification of oxidation of methionine and acetylation of the N-terminal. The MS mass tolerance was set at 4.5 ppm with a maximum number of five modifications. The false discovery rate was set at 0.01 for proteins and peptides and was run against a decoy revert database. Peptides required a minimum length of seven amino acids. The MS/MS tolerance was set at 0.1 Da for protein identification. The minimum score for modified and unmodified peptides was set at forty. At least two peptides were required for protein identification. The bioinformatics and statistical package Perseus was used to analyze the MaxQuant output. Contaminants identified by Perseus, such as keratin, were removed from the analysis. For

119 99 label free quantitation (LFQ), the intensity for each protein was transformed log2(x). A protein was considered identified in a treatment group if it was identified in at least 3 out of the 5 biological replicates Data analysis and bioinformatics The Gene Ontology (GO) Terms for biological process associated with identified proteins were determined using Gene Ontology Enrichment Analysis and Visualization Tool (GOrilla) [19, 20]. Visualization of enriched GO terms was accomplished using GOrilla comparing the isolated CLD proteomic profile to the entire mouse proteome obtained from the Uniprot protein database. The p value threshold was set at p<.01. Lipid related GO terms were identified and proteins associated with the lipid related GO terms were compiled and are here on referred to as Lipid related proteins. Visualization of protein interactions was accomplished using STRING version 10.0 [21]. We used the confidence view with a score of 0.4, indicating a medium confidence level Immunofluorescence imaging Four mice from low fat diet treatment group and the high fat treatment group were oil bolus. A small (5mm) section of the jejunum was harvested from the mice two hours after the dietary fat challenge and was frozen in optimal cutting temperature embedding media in 2-methyl butane cooled with dry ice. The tissue was stored at -80 C until

120 100 sections, fixed in 2% paraformaldehyde, permeabilized with 0.1% saponin, and blocked with 3% bovine serum albumin in PBS. The tissue was probed with previously validated antibodies for Plin3 [22] (a gift from Dr. Perry Bickel at the University of Texas Southwestern, Dallas TX, USA) and Plin2 [22]. The sections were also stained for neutral -difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY) (Life technologies, Grand Island NY, USA), a secondary AlexaFluor antibody (Life technologies), and for nuclei using 300 nm DAPI (Life technologies). The sections were imaged using a Nikon A1R confocal microscope (Nikon Instruments Inc., Melville NY, USA). Image processing was conducted using NIS- Elements C acquisition and analysis software Cardiac perfusion, fixation, and transmission electron microscopy Three mice from low fat diet treatment group and high fat treatment group, were was administered and two hours after the oil bolus, the mice were anesthetized using inhaled isoflurane and perfused with 1.5% glutaraldehyde in 0.1M sodium cacodylate via cardiac infusion. After fixation, a sample of the jejunum section of the small intestine was isolated, stained with osmium tetroxide, dehydrated, and embedded in resin. Ultrathin sections were stained with lead citrate and uranyl acetate and examined using a Tecnai T20 Transmission Electron Microscope (FEI, Hillsboro OR, USA).

121 Cytoplasmic lipid droplet size analysis Thirty cells were examined from (n=3 mice) from each treatment group. The cells analyzed were located in the middle section of the villi and at least 3 villi were measured per mouse. CLD diameter was measured using ImageJ. Image stitching was performed using Autostitch.. Data is expressed as mean ± SEM. Statistical significance was determined using a Type 3 mixed model for fixed effects using SAS v 9.4; P<0.05 was considered significant. Lipid droplet size distribution was analyzed by two-sample Kolmogorov Smirnov test; P<0.05 was considered significant. 3.4 Results Lipid accumulates in CLDs in enterocytes in response to an acute dietary fat lean and DIO mice Neutral lipids accumulate in CLDs in enterocytes of the jejunum section of the small intestine in lean and DIO mice in response to an acute dietary fat challenge (Fig 1). The representative micrographs highlight the size disparity of CLDs that accumulate in enterocytes of lean (Figure 1A) compared to DIO mice (Figure 1B).

122 CLDs in enterocyte of DIO mice are larger and have a different size distribution compared to lean mice in response to an acute dietary fat challenge The average CLD diameter was measured from 30 cells per mouse (n=3 mice) from each treatment group. No difference was observed in the number of CLD per cell in lean and obese mice in response to an acute dietary fat challenge (Figure 2a). DIO mice (black bars) have significantly greater CLD diameter compared to lean mice in response to an acute dietary fat challenge (Figure 2b). CLDs in enterocytes from DIO mice have a statistically significant size distribution compared to lean mice in response to an acute dietary fat challenge as determined by two-sample Kolmogorov Smirnov test (Figure 2c) Proteins were identified associated with isolated CLDs from lean and DIO mice in response to an acute dietary fat challenge To identify proteins which may contribute to the size distribution disparity between the lean and DIO mice in response to an acute dietary fat challenge, we identified proteins associated with isolated CLDs by LC MS/MS. A protein was considered identified in a treatment group if it was identified in at least 3 out of 5 biological replicates in at least one of the treatment groups. We identified a total of 375 proteins (Data not shown). Of the 375 identified proteins, a portion was found unique to a treatment group, some found at similar levels and others were found at differentiated levels (Figure 3).

123 Proteins identified associated with CLDs from lean and DIO mice are associated with a variety of biological processes The gene ontologies associated with the 375 identified proteins were compared to the complete mouse proteome and visualized using GOrilla (Data not shown). GOrilla highlights biological processes which are enriched within the CLD associated protein dataset. Biological processes highlighted in red indicate the most significant enrichment. The proteins identified in the current analysis are associated with a wide variety of biological processes (Data not shown). Biological process pathways that have the greatest enrichment include oxidation-reduction process (GO: ), cellular lipid catabolic process (GO: ), and organonitrogen compound metabolic process (GO: ). To identify proteins which may contribute to CLD size and lipid accumulation, we limited our analysis to the 81 (out of 375) proteins that are associated with lipid related processes as determined using GOrilla (Data not shown) Proteins associated with lipid related processes are found at differentially present levels and have been previously characterized associated with CLD in enterocytes proteomic or targeted protein analysis Of the 81 lipid related proteins identified in the analysis, 25 are found either unique or at differentiated present levels in lean and DIO samples (Table 1)Targeted STRING analysis of lipid related proteins identify interacting protein clusters associated with various lipid pathways.

124 104 To identify lipid functional pathways enriched in the CLD proteome, we conducted a STRING analysis of the 81 lipid related proteins (Figure 4). The STRING analysis identified protein clusters related to lipoprotein metabolism, TAG storage and mobilization, and fatty acid catabolism.the 25 proteins which are found unique or at higher levels in either lean or DIO samples are indicated by green and red boxes respectively and their relative levels are found in Table 1. Proteins found at higher levels in DIO samples are associated mainly with fatty acid catabolism, lipid synthesis, and lipid catabolism Plin3 and Plin2 localize to CLDs from lean and DIO mice in response to an acute dietary fat challenge. To confirm the presence of CLD associated proteins identified by CLD proteomic analysis, we used immunofluorescence microscopy of 2 proteins which were identified, Plin2 and Plin3 (Figure 5). Plin3 (A-B and E-F) is found at relatively equal abundance associated with CLDs from lean (A-B) and DIO (E-F) mice. Plin2 (C-D and G-H) is found at higher levels and forming a more distinct ring like structure around CLDs from DIO mice (G-H) compared to lean mice (C-D). These results validate that Plin2 and 3 associate with CLDs in lean and DIO mice and Plin2 is present at differentiated levels. An interesting observation was made regarding to the localization of Plin2 and Plin3, particularly in DIO samples.

125 Plin2 and Plin3 localize to different CLDs in different regions of the cell in DIO mice. Plin3 (A, C, and E) localizes to CLDs primarily in the apical region (above the nucleus) of the cell. Plin2 (B, D, and F) localizes to CLDs both in the apical and basolateral side of the cell. 3.5 Discussion We investigated the effects of DIO on enterocyte CLD morphology and associated proteins in response to an acute dietary fat challenge. DIO mice have larger CLDs with a greater maximal size compared to lean (Figures 1 and 2). 375 proteins were identified on CLDs from lean and DIO mice in response to an acute dietary fat challenge (Data not shown). A portion of the proteins were found unique to one of the treatment groups, a portion found at similar levels between the treatment groups, and some found at differentially expressed levels (Figure 3). The identified proteins represent a diverse range of proteins functions (Data not shown). Eighty one of the identified proteins are associated with lipid related pathways (Figure 4). Of the 81 lipid related proteins, 25 proteins are either unique to CLDs from DIO mice or found at differentially present levels in lean and DIO mice in response to an acute dietary fat challenge based on label free quantitation levels (Table 1). Plin3 is found associated with CLDs from lean and DIO mice at similar levels while Plin2 is found at increased levels on CLDs from DIO

126 106 mice compared to lean in response to an acute dietary fat challenge (Figure 5). Plin2 and Plin3 are found on different pools of CLDs in DIO mice in response to an acute dietary fat challenge (Figure 6). DIO alters CLD size which may contribute to the observed decreased TAG secretion rates in DIO compared to lean mice in response to an acute dietary fat challenge. High fat diet fed (non obese), ob/ob mice, and DIO mice have decreased TAG secretion rates after an olive oil bolus, when lipoprotein lipase has been inhibited with tyloxapol [9, 10]. Interestingly, while there is a decrease in TAG secretion, analysis of intestinal mucosa by either biochemical analysis or Coherent Anti-Scatter Ramen Spectroscopy[10] demonstrated no difference in TAG content between lean and DIO in response to an acute dietary fat challenge. The current study demonstrates an increased CLD size in DIO and lean mice in response to an acute dietary fat challenge by transmission electron microscopy. The difference between the current study and previous analysis of intestinal mucosa is the use of transmission electron microscopy to identify the subcellular localization of TAG in CLDs as compared to the analysis of the total enterocyte TAG content. Biochemical and CARS analysis cannot distinguish between subcellular localization of TAG in secretory or storage pathways in enterocytes. We hypothesize that the decreased TAG secretion rate and increased TAG storage in CLDs in DIO mice compared to lean in response to an acute dietary fat challenge may be an adaptation of DIO mice to chronic consumption of a high fat diet. Adaptation of enterocytes to chronic high fat diet feeding may allow the intestine to maintain efficient dietary fat uptake and absorption.

127 107 DIO alters CLD associated proteins in response to an acute dietary fat challenge. Previous observations identified Plin3 localized to the CLD of lean mice in response to an acute dietary fat challenge while Plin2 localized to the CLD of DIO mice under chronic high fat feeding [13]. This study is the first report of CLD associated proteins in enterocytes in DIO mice in response to an acute dietary fat challenge. Surprisingly, we identified Plin2 on CLDs from lean mice in response to an acute dietary fat challenge. In our previous report, we did not identify Plin2 associated with CLDs in enterocytes of lean mice in response to an acute dietary fat challenge [13]. The previous results are consistent with a lipid maturation hypothesis where Plin2 is thought of associating with more mature CLDs and Plin3 associates with nascent CLDs [22]. While it is unclear why Plin2 was identified on CLDs from lean mice in response to an acute dietary fat challenge, there are differences in the methodology in the current study compared to previous data generated in our laboratory. The current study uses a more sensitive proteomic technique to identify CLD associated proteins. Additionally, fresh frozen tissue used in the current study allows for imaging of a greater number of enterocytes compared to isolated enterocytes used in the previous report [13]. As seen in Figure 5, the Plin2 antibody generates a weaker signal around the CLDs in lean mice compared to DIO mice in response to a dietary fat challenge. It is possible that Plin2 was undetected in previous reports because it was present at levels that were below the level of detection. Lipid related proteins which were found identified either unique or at higher abundance associated with CLD from DIO mice compared to lean in response to an acute dietary fat challenge are similar to those found in models of hepatic steatosis induced by high fat feeding. To further our understanding of the effect of DIO on CLD associated

128 108 proteins we compared the findings of the current study to a previous report of liver CLD associated protein induced by high fat feeding [12]. The liver is an appropriate model system to compare the small intestine to in regards to lipid metabolism because they share several common features such as the ability to generate both CLDs and lipoproteins. Although both liver and intestine are capable of generating both triglyceride rich particles, the liver is often thought as a storage site for TAG while the small intestine is thought of as a through fare for TAG. A model of diet induced hepatic steatosis was used for comparison. Hepatic steatosis was induced by chronic high fat feeding (60 kcal% Bio- Serv, F3282 for 9 weeks) [12]. Several lipid related proteins which were found identified either unique or at higher abundance associated with CLD from DIO mice compared to lean in response to an acute dietary fat challenge which have also been previously reported in the models of hepatic steatosis include ApoC2, Faah, Abcd3, and Acadvl [12]. It is unclear if these proteins are present due to the effect of obesity on a CLD accumulation in response to an acute dietary fat challenge or if they are residual proteins present on CLDs induced by high fat feeding from the previous meal of high fat diet. The concept of proteomic differences between old CLD which are residual from the chronic consumption of high fat diet and newly formed CLDs generated in response to a meal is an interesting one, but this study does not address this issue. Different CLD pools have different protein composition in response to an acute dietary fat challenge. An unexpected observation made in this study was that Plin2 and Plin3 localize to different CLD pools in enterocytes of DIO mice in response to an acute dietary fat challenge (Figure 6). There are several lines of evidence suggesting that there are sub populations of CLDs in various cell types with different proteins associated with

129 109 them. While it has not been previously observed in the intestine, the perilipin family members associate with CLDs at different times during CLD maturation in adipocytes [22]. Additionally, members of the Cell death-inducing DNA fragmentation factor- -like effector (CIDE) family of proteins localize to different CLD subpopulations in hepatocytes[23]. Lastly, TAG synthesis enzymes localize to different CLD sub populations [24]. In particular AGPAT3 and GPAT4 colocalize on a subpopulation of CLDs. More recently, it has been hypothesized that two groups of lipid droplets exist in the cell. These CLDs are described as initial lipid droplets and expanding lipid droplets which have their own distinct protein compositions[25]. The current study provides supporting evidence for the possibility of different CLD subpopulations which have distinct sub-proteomes and unique metabolism. This study highlights the power of tandem mass spectroscopy to identify novel CLD associated proteins and compare the CLD proteome under different conditions, however it cannot distinguish between subpopulations of CLDs. This limitations highlights the need for microscopy techniques to confirm the presence of proteins on or around CLDs and if it the protein localizes to distinct CLD sub population. Proteins that associate with CLDs in lean and DIO mice in response to an acute dietary fat challenge are associated with a wide range of lipid related pathways (Figure 4 and Table 1). Proteins associated with lipid anabolic and catabolic pathways localize to CLDs. TAG synthesis enzymes localize to CLDs in Cos7 cells[24] including GPAT4, AGPAT3, ACSL1 and 3, and DGAT2. In the current study we identify Mogat2, Acsl5, and Dgat1 associated with CLDs from DIO mice (Table 1). Although we did not measure localized TAG synthesis, the presence of these TAG synthesis proteins on the CLD in

130 110 response to an acute dietary fat challenge indicates the potential for localized TAG synthesis. Enzymes that regulate CLD lipolysis have been well characterized in other cell types including hepatocytes [26] and adipocytes [27] but enzymes that conduct lipolysis in the intestine are under investigated. Several proteins which have been identified as lipases in other cell types have also been identified in this analysis. Lipe (Hormone sensitive lipase) was identified in CLDs from lean and DIO mice in response to an acute dietary fat challenge. Members of the carboxylesterase family have been identified on CLDs from lean and DIO mice. Some Carboxylesterase family members have been characterized as lipases, however the isoform Ces1f, which was identified on the CLD from DIO mice has not been characterized [28](Table 1), Arylacetamide deacetylase (Aadac) was identified on CLDs from lean and DIO mice but at higher levels on CLDs from DIO mice and has been characterized as a lipase in the HuH7.5 cells[29]. Additionally, members of the short-chain dehydrogenase/reductase family associate with CLD and may regulate TAG storage. Members of the short-chain dehydrogenase/reductase family including Dhrs1, HSD17B11, and HSD17B13 have been identified on the CLD from lean and DIO mice. The role of these proteins on the CLD is currently unknown, but evidence suggests that at least 1 family member, HSD17B13 is associated with human subjects with NAFLD and over expression results in increased accumulation of CLDs in mice[30]. The results from this study help generate a hypothetical model to generate hypotheses for further investigation (Figure 7) to explain the effect of DIO on intestinal lipid metabolism. DIO alters CLD morphology and associated proteins in response to an acute dietary fat challenge. Proteins found associated with the CLD are associated with

131 111 both anabolic and catabolic pathways. Proteins found associated with the CLD may regulate the balance between storage of dietary TAG and the mobilization of TAG to be utilized for chylomicron synthesis. The identification of proteins associated with TAG lipolysis and lipid synthesis associated with CLDs from DIO mice may indicate that mobilized FFA are being utilized for lipid synthesis directly on the CLD instead of being utilized for chylomicron synthesis. This is consistent with the previously reported decrease in TAG secretion observed in DIO mice in response to an acute dietary fat challenge. The identified CLD associated proteins provide potential targets for further investigation into the role of these proteins in dietary fat absorption We demonstrated that DIO alters CLD morphology and associated proteins in response to an acute dietary fat challenge. CLDs from DIO mice are larger with a different size distribution compared to lean mice. CLDs from DIO mice have some proteins in common and some unique compared to lean mice. Proteins found at higher levels or unique to CLDs from DIO mice are associated with lipid catabolic and anabolic pathways. Proteins identified in this analysis help expand upon the model of the effect of DIO on dietary fat absorption.

132 Acknowledgements We would like to thank member of the Bindley Bioscience Center for their help and expertise.this project was supported by the Indiana Clinical and Translational Sciences Institute, by Grant # UL1TR from the National Institutes of Health, National Center for Advancing Translational Sciences, Clinical and Translational Sciences Award (KKB), Grant # 7-13-IN-05 by the American Diabetes Association, Innovation Award (KKB),

133 113 Table 3-1 Label free quantitation of proteins associated with lipid related Gene Ontology terms. 375 identified proteins, 81 proteins are associated with lipid related Gene Ontology terms as identified by GOrilla (Data not shown). Of the 81 lipid related proteins, 25 were found at differentially present levels associated with CLDs from lean or DIO mice. Majority protein IDs Q3V2F7 Gene names Lean LFQ levels DIO LFQ level s T test P value Intestinal CLD Protein names Pathway Fatty acid-binding protein, liver Fabp [7] Lipid binding Q9DBZ6 ATP-binding cassette sub-family D member 3 Abcd Lipid transport, fatty acid oxidation P37040 NADPH--cytochrome P450 reductase Por [5] Oxidoreductase activity, FAO P51174 Long-chain specific acyl-coa dehydrogenase, mitochondrial Acadl Mitochondrial beta oxidation P50544 Very long-chain specific acyl-coa dehydrogenase, mitochondrial Acadvl Mitochondrial beta oxidation Q61425 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial Hadh [7] Mitochondrial beta oxidation Q3UUF0 Fatty-acid amide hydrolase 1 Faah Fatty acid catabolism Q91WU0 Carboxylesterase 1f Ces1f Triglyceride lipase Q99PG0 Arylacetamide deacetylase Aadac Triglyceride lipase Q922Z5 1-acylglycerol-3- phosphate O- acyltransferase ABHD5 Abhd [5, 6] Lipid homeostasis Q9QZQ8 Core histone macro- H2A.1 H2afy Regulation of lipid metabolism Q3UKA4 Alcohol dehydrogenase 1 Adh [7] Lipid metabolic process Q8C3C9 Fatty aldehyde dehydrogenase Aldh3a Long-chain-aldehyde dehydrogenase activity Q91Y74 CMP-Nacetylneuraminate-betagalactosamide-alpha- 2,3-sialyltransferase 4 St3gal [7] Ganglioside metabolism Q8N7N8 Hydroxymethylglutaryl- CoA synthase, mitochondrial Hmgcs Cholesterol/steroid biosynthesis

134 114 Table 3-1 Continued G5E850 Cytochrome b5 Cyb [7] Sterol biosynthesis Q9EQ06 P49586 Q80W94 H7BXC3 Q8VC30 E0CXN5 Estradiol 17-betadehydrogenase 11 Hsd17b [5, 6, 7] Steroid synthesis Choline-phosphate cytidylyltransferase A Pcyt1a [5] Phospholipid synthesis 2-acylglycerol O- acyltransferase 2 Mogat [4] Triglyceride synthesis Triosephosphate isomerase Tpi [5, 7] G3P metabolic process Bifunctional ATPdependent dihydroxyacetone kinase/fad-amp lyase Dak [7] G3P metabolic process Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic Gpd [7] G3P metabolic process Q8VCR2 17-beta-hydroxysteroid dehydrogenase 13 Hsd17b Oxidation-reduction process Q3UJG0 Apolipoprotein C-II Apoc Lipoprotein metabolism Q3U711 Perilipin-2 Plin [4, 5, 6, 13] Lipid storage

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145 125 CHAPTER 4. LONG CHAIN FATTY ACYL COA SYNTHETASE 5 ABLATION DECREASES TRIGLYCERIDE STORAGE IN ENTEROCYTES AND SECRETION IN RESPONSE TO AN ACUTE DIETARY FAT CHALLENGE COMPARED TO WILD TYPE MICE 4.1 Abstract Acyl-CoA synthetases (ACSs) activate fatty acids generating fatty acyl-coa which is further metabolized by the cell. One member of the ACS family, long chain fatty acyl Co-A synthetase 5 (Acsl5), is highly expressed in the intestine. Little is known of the role of Acsl5 in the metabolism of dietary fat in enterocytes. To understand the role of Acsl5 in dietary fat absorption, we challenged WT and Acsl5-deficient mice with acute and chronic dietary fat challenges. Despite no evidence of quantitative fat malabsorption in the absence of Acls5, we found significantly reduced TAG storage and secretion by enterocytes compared to WT mice. Interestingly, Acsl5-deficient consumed more food, but did not gain more weight compared to WT mice during two weeks of HFD feeding. Furthermore, we found that Acsl5 localizes to the nucleus after an acute dietary fat challenge. Together this data suggests that although Acsl5 is not essential for quantitative dietary fat absorption, it plays a role in intestinal metabolism of dietary fat.

146 Introduction ACSs initiate the first step of fatty acid metabolism in the cell by converting fatty acid to fatty acyl-coa [1].There are 25 known members of the ACS family with different tissue distribution patterns and fatty acid substrate preferences. Long chain fatty acyl CoA synthetases (Acsls) prefer long chain fatty acids with chain lengths of carbons. Acsl5 is highly expressed in the small intestine, liver, and brown adipose tissue [2]. Acsl5 has multiple subcellular localizations including mitochondria [3-6] and ER [2, 5]. More recently, Acsl5 was also identified [7, 8] and confirmed [8] to be associated with CLDs in enterocytes. Multiple areas of sub cellular localization of Acsl5 suggest Acsl5 may play multiple roles in cellular lipid metabolism. ACSs can have multiple functions in cells including fatty acid uptake and partitioning of fatty acids into different metabolic fates [9]. Over-expression of Acsl5 in McArdle-RH7777 cells, a rat hepatoma cell line, increased fatty acid uptake, and preferentially directed exogenous fatty acids toward TAG synthesis [3]. In a separate study, knocking down Acsl5 by sirna in rat primary hepatocytes decreased fatty acid channeling toward anabolic pathways [10]. In this model, knocking down Acsl5 decreased incorporation of fatty acids into TAG, phospholipids, and cholesterol esters, but did not affect fatty acid uptake. The decrease in incorporation of fatty acids into complex lipids was accompanied by decreased CLD formation and lipoprotein secretion. Lastly, Acsl5 knockdown cells have increased fatty acid oxidation. These results suggest Acsl5 directs fatty acids toward anabolic pathways.

147 127 To investigate the role of intestinal Acsl5 in dietary fat absorption, Meller et al. generated an Acsl5-deficient mouse model[11]. Acsl5-deficient mice had a 60% decrease in total ACS activity in the jejunum compared to WT mice. Despite the decrease in ACSL activity, Acsl5-deficient mice had similar TAG secretion in response to an acute dietary fat challenge compared to WT mice. Additionally, Acsl5-deficient mice had similar growth curves when fed a Western Diet (17% protein, 49% carbohydrates, 21% fat) compared to WT mice. This data suggests that Acsl5 does not play a role in dietary fat absorption by the small intestine. A new Acsl5-deficient mouse model has been generated with an improved metabolic phenotype compared to WT mice. Whole body deletion of Acsl5 resulted in a reduction of total Acsl activity by 80% in jejunal mucosa, 50% in liver, and 37% in brown adipose tissue lysates [12]. Overall Acsl5-deficient mice had decreased fat mass, increased metabolic rate, increased respiratory quotient, reduced serum TAG, and improved insulin sensitivity. This is the mouse model that was used for this study. To determine the role of Acsl5 in dietary fat absorption we challenged Acsl5=deficient and WT mice with acute and chronic dietary fat challenges. We hypothesized that Acsl5 directs fatty acids toward TAG synthesis and that the absence of Acsl5 would result in a decrease in enterocyte TAG storage in CLDs and a decreased secretion rate in response to an acute dietary fat challenge. To test this hypothesis we analyzed TAG storage in the jejunum section of Acsl5-deficient mice compared to WT mice in response to an acute dietary fat challenge. Additionally, we measured TAG secretion in response to an acute dietary fat challenge in Acsl5-deficient and WT mice. Lastly, to determine if Acsl5

148 128 deficiency results in fat malabsorption as indicated by steatorrhea, we fed Acsl5-deficient and WT mice a high fat diet and measured fecal lipid content. 4.3 Materials and methods Diets and mice All procedures were approved by the Purdue Animal Care and Use Committee. A conditional, Acsl5-deficient mouse line was generated by Dr Andrew Greenberg at Tuft s University and backcrossed to C57Bl6J line as previously reported (Bowman 2016). All mice were maintained on a 12- hour light/dark cycle (6AM/6PM) with ad libitum access to food and water unless indicated. Mice were fed a chow diet or where indicated, a high fat diet. This chow diet (PicoLab 5043, Lab Diets, Richmond, IN) has 62.1% of calories from carbohydrate (starch), 24.7 % from protein, and13.2% from fat. The high fat diet (D12492, Research Diets Inc, New Brunswick, NJ) has 20% calories from carbohydrate (35% sucrose, 65% from starch), 20% from protein, and 60% from fat (lard). Mice were euthanized via CO2 asphyxiation Tissue imaging Mice were fasted for 4 hours at the beginning of the light cycle. Mice were then olive oil bolus via oral gavage. Mice were euthanized 2 hours after the oil bolus by CO2 asphyxiation. The small intestine was isolated and flushed with ice cold PBS and divided into 5 equal sections. Sections 2 and 3, representing the jejunum,

149 129 were analyzed. A small 1 cm2 section of the intestine was taken and cut longitudinally exposing the lumen. The section was embedded in optimal cutting temperature media (OCT) and frozen in 2-Methylbutane cooled by dry ice. Embedded tissue was stored at - 20 C until sectioned. plus charged microscopy slides (Fisher scientific, Hanover Park, IL, USA). The tissue was fixed in 2% paraformaldehyde, and permeabilized with 0.1% saponin. The tissue was probed with a previously validated antibody Acsl5 (a gift from Dr. Andrew Greenberg from Tufts University, Boston MA, USA). The sections were also stained for neutral -difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY) (Life technologies, Grand Island NY, USA), a secondary AlexaFluor antibody (Life technologies), and for nuclei using 300 nm DAPI (Life technologies). The sections were imaged using a Nikon A1R confocal microscope (Nikon Instruments Inc., Melville NY, USA). Image processing was conducted using NIS- Elements C acquisition and analysis software TAG secretion To assess TAG secretion in response to an acute dietary fat challenge, mice were fasted for 4 h, starting at the beginning of the light cycle. Mice were then injected with tyloxapol (500 mg/kg) IP to block lipases required for clearance of TAG from blood. Thirty minutes after tyloxapol injection, blood was collected via submandibular bleed for analysis of plasma TAG concentrations (Time 0). Mice were then immediately gavaged

150 130 gavage for plasma TAG analysis. Plasma TAG concentrations were determined by Wako L-Type TG M kit (Wako Chemicals USA) High fat feeding and food intake analysis Mice were fed a high fat diet (60% kcal) for 2 weeks and body weights determined weekly. To determine food intake, mice were housed individually in metabolic cages and food was weighed daily for the last 4 days of high fat feeding Fecal lipid analysis To assess fecal lipid content, feces were collected from mice for the last 4 days of the 2 week high fat diet feeding. Feces were dried, weighed, ground using mortar and pestle, and 0.5g was used for lipid analysis. Lipids were extracted using the Folch extraction (2:1 chloroform: methanol) procedure. Percent fecal lipid content was determined: (mg extracted lipid/mg dried feces)* Results Acsl5-deficient mice accumulate less neutral lipids in CLDs in response to an acute dietary fat challenge compared to WT mice. To assess the effect of Acsl5 on enterocyte neutral lipid storage in response to an deficient mice. Two hours after the acute dietary fat challenge, the small intestine was

151 131 isolated, sectioned and stained for Acsl5, neutral lipids, and nuclei (Figure 1). Acsl5 is present in enterocytes of WT mice (Fig 1A) but absent in Acsl5-deficient mice (Fig 1D). WT mice had greater accumulation of neutral lipids (orange) (Fig 1B) compared to Acsl5-deficient mice (E) in response to an acute dietary fat challenge Decreased TAG secretion in Acsl5-deficient mice compared to WT mice. olive oil bolus was administered to WT and Acsl5-deficient mice pretreated with a lipase inhibitor, that prevents clearance of TAG from blood, Tyloxapol. Blood was collected at T=0, 2, and 4 hours after the olive oil bolus. TAG secretion at 4 hours after an acute dietary fat challenge was significantly reduced in Acsl5-deficient compared to WT mice (Fig 2) Acsl5-deficeint mice have increased food intake without differences in body weight or fecal lipid content compared to WT mice when fed a high fat diet. To assess the effect of Acsl5 deficiency on weight gain, food intake, and quantitative dietary fat absorption, WT and Acsl5-deficient mice were fed a HFD (60% kcal) for two weeks body weight, food intake, and fecal lipid content were determined. WT and Acsl5 had similar body weight curves during this time period (Fig 3a). Despite similar body weight gain, Acsl5 deficient mice consumed significantly greater amounts of food compared to WT mice during the last 3 days of HFD feeding (Fig 3b) with similar excretion of lipid in feces (Fig 3c).

152 Nuclear localization of Acsl5 in enterocytes in response to an acute dietary fat challenge in WT mice. To assess Acsl5 subcellular location in enterocytes in response to an acute dietary l bolus was administered to WT mice by oral gavage. Samples from the jejunum section of the small intestine were isolated before the oil bolus (after a 4 hour fast) and two hours after the oil bolus. The small intestine was isolated, sectioned, and probed for Acsl5 (green) and counter stained for lipid (Bodipy, orange) and nuclei (DAPI, blue) (Figure 4). Before the oil bolus, Acsl5 is found dispersed in the cell (Figure 4A,B). Two hours after the oil bolus, Acsl5 distinctly localizes to nuclei (Figure 4C-F). 4.5 Discussion To understand the role of Acsl5 in dietary fat absorption, we challenged WT and Acsl5-deficient mice with acute and chronic dietary fat challenges. Despite no evidence of quantitative fat malabsorption in the absence of Acls5, we found significantly reduced TAG storage and secretion by enterocytes compared to WT mice. Interestingly, Acsl5- deficient consumed more food, but did not gain more weight compared to WT mice during two weeks of HFD feeding. Furthermore, we found that Acsl5 localizes to the nucleus after an acute dietary fat challenge. Together this data suggests that although Acsl5 is not essential for quantitative dietary fat absorption, it plays a role in intestinal metabolism of dietary fat.

153 133 Members of the ACSL family have been identified associated with CLDs and contribute to CLD metabolism [13]. Recent proteomic analyses of isolated CLDs from enterocytes identified [7, 8] and confirmed [8] Acsl5 localization to CLDs after a lipid challenge. We found less TAG storage in CLDs in Acsl5-deficient mice in response to an acute dietary fat challenge compared to WT mice. These results are similar to those observed in primary rat hepatocytes where Acsl5 knockdown resulted in a decrease in TAG storage [10]. Taken together, these results suggest that Acsl5 works in concert with TAG synthesis machinery on the CLD to generate TAG for storage. Blood TAG levels in the postprandial state are a balance between TAG secretion from the intestine and clearance by peripheral tissues. We found that Acsl5-deficient mice have reduced TAG secretion in response to an acute dietary fat challenge compared to WT mice. These results are in contrast to previous reports from a different mouse model that is also deficient in Acsl5 where TAG secretion rates were found to be similar to WT mice [11]. There are several important differences between the two studies. The differing genetic backgrounds might explain some of the observed phenotypic differences. The Bowman Acsl5-deficient mice were on a pure C57BL6J background while the Meller Acsl5-deficient mice were on a mixed C57BL6J/129sJ background. The Bowman Acsl5-deficient mice had reduced total ACSL activity in liver extracts consistent with previous published studies showing that sirna knockdown of ACSL5 in isolated hepatocytes significantly reduced total ACSL activity [10]. Meller et al. did not report the expression of all of the other ACSL isoforms in their mouse livers, thus, another ACSL isoform may have compensated for any reductions in ACSL activity. The two studies also differ in experimental methodology. The first issue regarding the TAG secretion assay is

154 134 the amount of dietary fat which was used to challenge the mice. The Meller mouse model live oil used in the! " stress the intestine to delineate differences between Acsl5 deficient and WT mice. The second issue regarding the TAG secretion assay is the time period of assessment. The Meller mouse model collected blood samples until 90 minutes. In the current study, we collected samples 4 hours after the dietary fat challenge and it was only at the 4 hour time point that TAG secretion differs between Acsl5 deficient and WT mice. These data suggests that Acsl5 regulates dietary fat secretion in the small intestine, however under specific physiological conditions. Deficiencies in dietary fat uptake and secretion are often accompanied by fat malabsorption. Since Acsl5 deficient mice have a decrease in TAG storage and TAG secretion compared to WT mice, we analyzed fecal lipid content in mice fed a high fat diet to assess steatorrhea. Surprisingly, Acsl5 deficient mice do not have an increase in fecal lipid content compared to WT mice when fed a high fat diet. One hypothesis to explain this observation is Acsl5 deficient mice take up fatty acids in different regions of the small intestine compared to WT mice. Acsl5 deficient mice may have lower rates of fatty acid uptake in enterocytes in the upper region of the intestine; instead take up dietary fatty acids in smaller quantities in different regions. By diffusing dietary fat uptake along the length of the intestine, Acsl5 deficient mice can have decreased TAG secretion rates and TAG storage without fat malabsorption. In this study we only assessed TAG storage in the jejunum section of the small intestine and only assessed TAG

155 135 secretion and uptake on a whole body physiological level. Future studies to assess fatty acid uptake and TAG storage and secretion at a cellular level in specific regions of the small intestine are important to clarify this issue. We also investigated weight gain and food intake of high fat diet in Acsl5 deficient mice and WT mice. Acsl5-deficient and WT mice consumed similar quantities of food when fed a chow diet [12]. When Acsl5-deficient mice were fed a HFD however, Acsl5-deficient mice consumed significantly more food compared to WT mice. Increased food intake may be attributed to a number of factors and the factors which influence an increase in food intake on a HFD in Acsl5 deficient compared to WT mice have not been studied. One hypothesis for increased food intake in Acsl5 deficient mice compared to WT mice is an increased circulating level of FGF21[12]. Overexpression of FGF21 increases food intake when mice are fed chow or high fat diets [15]. Acsl5-deficient mice may consume more high fat diet compared to WT mice due to their increased levels of serum FGF21. Despite the increase in food intake, Acsl5 deficient mice had similar weight gain on high fat diet compared to WT mice when fed for a 2 week period. The increase in caloric intake may be offset by the increased energy expenditure in Acsl5- deficient mice compared to WT mice [12]. A surprising observation made during imaging of Acsl5 in response to an acute dietary fat challenge was the dramatic change in subcellular localization of Acsl5. In the before an oil bolus, Acsl5 is found dispersed in the cell, however when WT mice are given an acute dietary fat challenge, Acsl5 distinctly localizes to the nucleus of enterocytes. Acsl5 is known to localize to mitochondria and CLDs in enterocytes [4, 8] however nuclear localization pattern of Acsl5 has not been previously observed in

156 136 enterocytes. The presence of long chain fatty acyl-coa and ACSLs has been detected in the nucleus of rat liver [16-20]. Acyl-CoAs are thought to be present within the nucleus and serve as ligands for transcription factors that may regulate expression of proteins involved in TAG storage and secretion. Acyl-CoAs may be transported into the nucleus or based on this observation generated within the nucleus. This unexpected discovery represents a very interesting area for future research. Acsl5 plays a role in intestinal lipid metabolism in response to dietary fat. Despite no evidence of quantitative fat malabsorption in the absence of Acls5, we found significantly reduced TAG storage and secretion by enterocytes compared to WT mice. Interestingly, Acsl5-deficient consumed more food, but did not gain more weight compared to WT mice during two weeks of HFD feeding. Furthermore, we found that Acsl5 localizes to the nucleus after an acute dietary fat challenge. Together this data suggests that although Acsl5 is not essential for quantitative dietary fat absorption, it plays a role in intestinal metabolism of dietary fat. 4.6 Acknowledgments We would like to thank Dr. Andrew Greenberg and members of his lab for help and guidance. In particular we would like to thank, Dr. Tom Bowman and John Griffen for their help. This work was supported by the Purdue Research Foundation, American Diabetes Association (7-13-IN-05), the Indiana Clinical and Translational Sciences Institute funded, in part by Grant Number Grant # UL1TR from the National Institutes of

157 137 Health, National Center for Advancing Translational Sciences, Clinical and Translational Sciences Award. NIEHS (UO1-ES , RO3-ES-0227), NIDDK (R01 DK ), NIDDK-Boston Nutrition Obesity Research Center (P30-DK-46200), T32 DK , and the U.S. Department of Agriculture, Agricultural Research Service, under agreement no

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159 Plasma TG (mg/dl) * WT Acsl5 -/ Time post oil bolus (Hrs) Figure 4-2 Decreased TAG secretion in Acsl5-deficient mice compared to WT mice. Postprandial TAG secretion rate was measured in WT and Acsl5 -/- mice. Mice were injected with 500 mg/kg Tyloxapol into the interperitoneal cavity to inhibit lipoprotein lipase activity. Thirty minutes after Tyloxapol injection, the mice were bled (T=0) and post oil bolus. Data represented as mean +/- SEM. Asterisk denotes significant difference at the time point (Student T test, p<0.05). n= 3-5 male mice.

160 140 A Body Weight (g) B Food intake (g) * * * Days of HFD feeding Day C 3 Fecal lipid (%) WT Acsl5 -/- Figure 4-3 Acsl5-deficeint mice have increased food intake without differences in body weight or fecal lipid content compared to WT mice when fed a high fat diet No different in body weight between Acsl5-/- and WT mice on a high fat diet for 2 weeks. Body weights of Acsl5 -/- and WT mice fed a high fat diet (60% kcal) were monitored for 2 weeks (A). No difference in body weight was determined using a Student t test (p<.05) Data represented as mean +/- SEM. n= 4-5 mice. Increased food intake of Acsl5 - /- mice compared to WT mice when fed a high fat diet (B).Food intake was monitored during the last 4 days of a two week period of high fat diet (60% kcal) feeding. Acsl5 -/- mice had increased food intake compared to WT mice as determined by a Student t test (* p<.05). Data represented as mean +/- SEM n= 4-5 mice. No difference in fecal lipid content in Acsl5 -/- compared to WT mice after 2 weeks of high fat feeding (C). Acsl5 -/- and WT mice were fed a high fat diet (60% kcal) for 2 weeks. Fecal samples were collected during the last 4 days of feeding. Lipid content was determined by Foch extraction of ground feces. Data represented as mean +/- SEM. No difference in fecal lipid content levels was determined by Student T Test p <.05 n=4-5 male mice.

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