Outline. Outline. Introduction. Appendix 88. Introduction Experimental design Results Conclusion

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1 Appendix Ryan Waters Introduction Experimental design Results Conclusion Introduction Experimental design Results Conclusion Introduction Foot and mouth disease - High morbidity, Low mortality Tiger heart Epitheliotropic virus Acute morbidity associated with epitheliotropism Open Session of the EuFMD Research Group, Vienna (Austria) 9 September October

2 Appendix Low Mortality rate? Reported high mortality rates in neonatal animals - Myocarditis: Reported outbreaks with high mortality in non-neonates: Domestic and wild animals Associated with certain strains i.e O Iran Need for understanding basic pathogenesis- - Is virus replicating? - Where? - any subclinical heart damage? Introduction Experimental design Results Conclusion Experimental design Neonatal lambs contact challenged, control Euthanased,,,,7 Introduction Experimental design Results Conclusion Neonatal lambs ICBI, control Eutnanased,,,7, Is the Virus replicating? Dpi LA LM found dead DPI LM found dead DPI LA Dpi Open Session of the EuFMD Research Group, Vienna (Austria) 9 September October

3 Appendix WHERE IS VIRUS REPLICATING? WHERE IS VIRUS REPLICATING? Subclinical heart damage LA7 - Lamb killed dpi LA79 - Lamb killed dpi ICBI lambs only considered as all were sub clinical Combination of immunofluorscence and Cardiac Troponin T assay Copies viral RNA/ml serum (log) LA77 - Lamb killed dpi LB79 - Lamb killed 7 dpi LA - Lamb killed dpi LA - Lamb killed 7 dpi Degrees centigrade = Antibody first detected by LPBE LB7 - Lamb killed dpi LA7 - Lamb killed dpi ERZZ PCR Immuno DAY heart heart Lamb id DAY DAY DAY DAY DAY DAY DAY 7 DAY DAY 9 DAY LOG LA Killed Killed Killed Killed Killed Killed Killed Killed Killed Killed Killed NEG NEG LB Killed Killed Killed Killed Killed Killed Killed Killed Killed Killed Killed NEG NEG LA7 NEG NEG Killed Killed Killed Killed Killed Killed Killed Killed Killed NEG. LA79 NEG NEG Killed Killed Killed Killed Killed Killed Killed Killed Killed.9 NEG LA77 NEG NEG NEG NEG Killed Killed Killed Killed Killed Killed Killed 7.7 NEG LA NEG POS + POS + POS + Killed / POS Killed Killed Killed Killed Killed Killed. Pos LB79 NEG NEG NEG POS + POS + POS + POS + Killed Killed Killed Killed 7. Pos / POS LA NEG POS + POS + POS + POS + POS + POS + Killed / POS Killed Killed Killed. NEG LA7 NEG NEG POS + POS + POS + POS + POS + POS + POS + POS + Killed NEG LB7 NEG POS + POS + POS + POS + POS + POS + POS + POS + POS + Killed / POS NEG / animals had detectable ctnt = % Open Session of the EuFMD Research Group, Vienna (Austria) 9 September October

4 Appendix LA77 Killed dpi 7 LA Killed dpi Dpi LA LB79 Killed 7dpi LA Killed 7 dpi LB7 Killed dpi LA7 Killed dpi 7 Arbititary levels of ctnt Days post innoculation LA Dpi Dpi LA Dpi LA Conclusions FMDV replicates in the hearts of neonatal lambs, infecting only the cardiomyocytes Direct damage of cardiac myocytes, extensive-diffuse replication Death Subclinical heart damage relatively common Other work In vitro culture of cardiac myocytes Characterisation of inflammatory cell infiltrate Open Session of the EuFMD Research Group, Vienna (Austria) 9 September October

5 Appendix Acknowledgments Zhidong Zhang Claudia Doel Eoin Ryan Bryan Charleston Pippa Hawes Jennifer Simpson Eoin Ryan Nick Juleff Haru Takamatsu Joe Brownlie Open Session of the EuFMD Research Group, Vienna (Austria) 9 September October

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