The work of the WRLFMD in relation to regional networks. Reference Laboratory for FMDV
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1 The work of the WRLFMD in relation to regional networks Reference Laboratory for FMDV
2 WRLFMD - GOODBYE TO: Nigel Ferris January 2012 Geoff Hutchings July 2012 Claudia Doel March 2013 Miki Madi June 2013 Jef Hammond July 2013 Yanmin Li August 2013
3 WRLFMD NOW David Paton John Bashiruddin Debi Gibson KITS Don King Valérie Mioulet VIROLOGY Bryony Armson Britta Wood Ashley Gray Veronica Fowler Anna Ludi SEROLOGY Barsha Thappa Vacancy Dx/R Dx/R Dx/R R Abid Bin Tarif Kelly Adams Ginny Wilsden Pip Hamblin Bob Statham PTS VM Nick Knowles SEQUENCING Graham Freimanis Jemma Wadsworth Kasia Bankowska Begoña Valdazo-Gonzalez David King NGS VP1 FGS
4 COUNTRIES October October 2013 Pool 3 PAT Bhutan Pool 1 Pool 5 Hong Kong SAR Clinical samples Viral sequences Pool 7 Pool 4 Pool 2 Sri Lanka Pool 6 Reports for these samples can be found at
5 RESULTS FOR SAMPLES SUBMITTED TO WRLFMD IN THE PAST 12 MONTHS O A SAT 1 SAT 2 ASIA-1 NVD Total: 337 samples submitted and processed (46 in progress) 219 isolates 269 samples collected in 2013 rrt-pcr: 260 positive (79%) NVD = no virus detected (negative by VI and Ag-ELISA)
6 RESULTS FOR SAMPLES SUBMITTED TO WRLFMD IN THE PAST 12 MONTHS FMDV type O FMDV type A Bhutan PAT Hong Kong SAR Si Lanka FMDV type Asia-1 FMDV type SAT1 FMDV type SAT2
7 TRAINING HELD AT WRLFMD, PIRBRIGHT Year Participants Course Countries Diagnostic course Brazil, Jordan, New-Zealand, Tanzania, Uganda, Zambia, Zimbabwe Vaccine matching workshop USA Diagnostic course Brazil, Ethiopia, New-Zealand, Spain, Uganda, Zimbabwe Diagnostic course New Zealand, Nigeria, Russia, Sudan, Uganda Vaccine matching workshop Belgium, Kenya, Russia, Nigeria, Thailand, Botswana
8 PROFICIENCY TEST (PTS) Prepared and shipped out annually Results from Phase I reported in Brescia: 21 laboratories participated Compared VI and VNT methods Reported issues in logistics and sample shipment 2005 Pilot study with 5 labs to test feasibility of including virus detection assays (VI and RT-PCR) Phase XIX to include molecular assays for virus detection 2013 Phase XXVI
9 PTS CURRENT FORMAT Participants supplied with up to 4 separate panels: Panel 1: infectious material from 2 cases of suspected vesicular disease for virus detection (FMDV and SVDV) Panel 2: non-infectious material from cattle or pigs for virus genome/antigen detection (FMDV and SVDV) Panel 3: non-infectious material for FMD serology Panel 4: non-infectious material for SVD serology Each sample is randomly assigned a number and labeled accordingly
10 d - m a p s. c o m PTS 2012 participation 76 laboratories in 72 countries invited 57 laboratories in 54 countries received at least one panel Results returned: EU and acceding member countries (26) EUFMD sponsored countries (18) Self-funded countries (10) (where panels were sent but no results returned, country is not counted as a participant (6))
11 EU (n=12) EUFMD (n=6) Other (n=5) EU (n=12) EUFMD (n=6) Other (n=5) EU (n=12) EUFMD (n=6) Other (n=5) EU (n=12) EUFMD (n=6) Other (n=5) EU (n=12) EUFMD (n=6) Other (n=5) EU (n=12) EUFMD (n=6) Other (n=5) Correct results (%) PTS 2012 RESULTS PANEL 1 Infectious material from 2 cases of suspected vesicular disease for virus detection (FMDV and SVDV): 100 Panel 1 - Overall results VI and Ag-ELISA Molecular assays 0 Epi FMDV O SKR/4/2010 Epi -ve Faeces FMDV O SKR/4/2010 Epi SVDV ITL/7/07 Epi -ve2 Faeces SVDV ITL/8/07
12 EU EUFMD Other EU EUFMD Other EU EUFMD Other EU EUFMD Other EU EUFMD Other EU EUFMD Other Correct results (%) PTS 2012 RESULTS PANEL 2 Non-infectious material from cattle or pigs for virus genome/antigen detection (FMDV and SVDV) 100 Panel 2 results FMD O Pak/28/2010 FMD A Tur/28/2010 FMD Sat 2 Bot/2/2010 SVD ITL/07/07 Asia 1 Bar 9/2009 MEM Ag-ELISA and LFD (EU n=20; EUFMD n=10; Other n=9) Molecular assays (EU n=24; EUFMD n=11; Other n=8)
13 d - m a p s. c o m PTS 2013 SO FAR 86 countries/labs invited 61 countries/labs requested participation 54 shipments sent out over August-October 45 confirmations of receipt (only) 40 sets of results received so far Panels requested 2013: EU and acceding member countries (26) EUFMD sponsored countries (22) Self-funded countries (8) FMD regional labs
14 WISH LIST Under-represented pools/countries: increase sample numbers or reports/results Training: more targeted to needs and individual labs. PTS: increase participation from regional ref labs and other labs. improve feedback, should be as practical as reasonably possible. QA and biosecurity processes: improve in local labs without it becoming an unreasonable burden. Good processes more important than accreditation. improve understanding of the processes required for international shipments of infectious and potentially infectious material. What do the regulations mean in practice? Keep improving and updating lab and field assays. Must be as practical as needed by the different end-users (vets, labs ). Improve access to and dissemination of information whilst ensuring that it is not corrupted (sequence database).
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