Vaccine Application: Experience of a Daily Management. Ceva Poultry Vaccinology Summit-Budapest April 30 th 2015

Transcription

1 Vaccine : Experience of a Daily Management Ceva Poultry Vaccinology Summit-Budapest April 30 th 2015

2 Vaccine 1 - Vaccine 3 Sub-cutaneous. 2 In-Ovo 4 Spray 2 Vaccinology Summit Budapest

3 Vaccine 1 - Vaccine Vaccine Storage Room Process Process Conditions 3 Vaccinology Summit Budapest

4 Vaccine 1 - Vaccine TOPIC MAIN FAILURES Vaccine Storage Storage temperature Fridge organization process Room Conditions Equipement settings Thawing process Contamination Liquid Nitrogen management Ventilation system Biosecurity and hygiene 4 Vaccinology Summit Budapest

5 Vaccine : Storage Temperature Vaccine Storage 5 Vaccinology Summit Budapest

6 Vaccine : Temperature monitoring Vaccine Storage 6 Vaccinology Summit Budapest

7 Vaccine : Temperature monitoring Temperature monitoring To avoid cold-chain failures Vaccine Storage It needs to be monitored on a regular basis to be an efficient method of control New solutions are available for monitoring 7 Vaccinology Summit Budapest

8 Vaccine : Focus on Marek vaccines Liquid Nitrogen Management Dewars need to be controlled and refilled regularly Operator Safety must be the first priority Process 8 Vaccinology Summit Budapest

9 Vaccine : Focus on Marek vaccines Process PFU Titre of HVT cell-associated vaccines variation depending on different thawing times Controls (30 sec) 5 mn 10 mn 15 mn Thawing time Halvorson DA and DO Mitchell, 1979, Av Dis 24; Vaccinology Summit Budapest

10 Vaccine : Focus on Marek vaccines Process ANTIBIOTIC TYPE % TITER LOSS Gentamicin Sulfate 32,5 Lincomicin Spectinomycin 18,3 Ceftiofur Sodium 9,2 Low quality standard blue dye also may have an impact on titer loss up to 20% 10 Vaccinology Summit Budapest

11 Vaccine : Focus on Marek vaccines Thawing temperature: C Thawing time: seconds Vaccine bag temperature: room temperature Process Always respect the following order : 1- Additive 2- Immune-complex vaccines 3- Blue dye 4- Regular live vaccines 5- Vector-vaccines 11 Vaccinology Summit Budapest

12 Vaccine : General Rules 1 - Vaccine Vaccines need to be stored inside the fridge directly after the reception. Fridge needs to be well organized (FIFO). Vaccine storage temperature between 2 to 8ºC (F) Vaccine needs to be prepared in aseptic conditions The vaccine preparation room is not a storage room 12 Vaccinology Summit Budapest

13 1 - Vaccine 2 In-Ovo 13 Vaccinology Summit Budapest

14 In-Ovo : Reminder CONCEPT Vaccination Age Optimal Vaccination Efficacy Maximum Speed In-Ovo 17,5 to 19,2 days ED (18,5 avg.) +98% eggs/hour Maximum Output/Day eggs/day Vaccines Process Inmune-complex, Vectorvaccines, Marek vaccines 14 Vaccinology Summit Budapest

15 In-Ovo : Key Factors 2 In-Ovo Eggs quality Embryo Hygiene & development Process Biosecurity 15 Vaccinology Summit Budapest

16 In-Ovo : Key Factors TOPIC MAIN FAILURES Eggs quality Poor egg shell quality Dirty eggs and floor eggs 2 In-Ovo Embryo development Hygiene and Biosecurity Heterogenous embryo development Wide hatch-window Vaccine bag contamination Equipment contamination Aspergillus sp. impact 16 Vaccinology Summit Budapest

17 In-Ovo : Egg Quality 17 Vaccinology Summit Budapest

18 In-Ovo : Embryo Development Ideal Medium Poor Early Embryo development days Vaccinology Summit Budapest

19 1 - Vaccine 3 Sub-cutaneous. 2 In-Ovo 19 Vaccinology Summit Budapest

20 Sub-cutaneous 3 Sub-cutaneous. Equipment Operator Process 20 Vaccinology Summit Budapest

21 Sub-cutaneous : Key Factors 3 Sub-cutaneous. TOPIC MAIN FAILURES Equipment Equipment settings Needle settings Needle replacement Preventative Maintenance Operator DOC handling Vaccination technic Speed Vaccine bag management 21 Vaccinology Summit Budapest

22 Sub-cutaneous : Injection Quality Sub cutaneous injection failure : wet with blood without vaccine injected at the wrong locations Equipment Injection quality= Nb of DOC per crate - nb injection failure 22 Vaccinology Summit Budapest

23 Sub-cutaneous : Equipment Impact Parameters Sub Cutaneous Injector- Benchmarking(%) Date Number of Countries % Number of hatchery 234 Equipment 99% 98% 97% 96% 95% 94% 93% 92% 98% DOVAC 95% OTHERS Process Number of audits 4401 Number of DOC evaluated CV CV (%) DOVAC 3 OTHERS Vaccinology Summit Budapest

24 % d Audits Sub-cutaneous : Equipment Impact Audits distribution according to injection quality Equipment 30,0% 25,0% 20,0% 15,0% 10,0% 5,0% 0,0% Parameters Date Number of Countries 25 Number of hatchery 234 Number of audits 4401 Number of DOC evaluated Process Injection Quality (%) DOVAC OTHERS/MANUAL 24 Vaccinology Summit Budapest

25 Sub-cutaneous : Data Distribution of Injection failure among DOC % of wet DOC 56% % of DOC with blood 21% Equipment % of DOC with wrong Location % of DOC with no Vaccine 7% 15% Process 0% 10% 20% 30% 40% 50% 60% 25 Vaccinology Summit Budapest

26 Sub-cutaneous 3 Sub-cutaneous. VACCINATION FAILURE No Vaccine IMPACT No protection in the field Vaccine waste MAIN CAUSE Equipment malfunction Wet Fluff Low or no protection Vaccine waste Equipment settings, DOC handling Wrong location Blood Low absorption of the vaccine Risk of damages of vascular system Risk of damages on digestive tract Infection of damaged tissues Process Higher first week mortality Equipment settings, DOC handling Equipment settings Killed Injection at crane or back-spine Cull chicks; Mortality Equipment settings 26 Vaccinology Summit Budapest

27 Sub-cutaneous : 3 Sub-cutaneous. Choosing the right equipment is a must to achieve a good vaccination quality level. Needle settings must be monitored and controlled regularly. Sub-cutaneous vaccination is an operator dependent process. Process 27 Vaccinology Summit Budapest

28 Vaccine 1 - Vaccine 3 Sub-cutaneous. 2 In-Ovo 4 Spray 28 Vaccinology Summit Budapest

29 Spray : Hatchery Spray Process Evaluation 4 Spray TOPICS Equipment Set-up Equipment performance Process & Environment Type of equipment Droplet size Pressure level Cap protection CONTENTS Real volume of vaccine being sprayed Droplet homogeneity Vaccine waste evaluation Ambient parameters (T, RH) Operator impact Cleaning and disinfection process 29 Vaccinology Summit Budapest

30 Spray : Hatchery Spray Process Evaluation TOPIC EQUIPMENT SET UP HATCHERY 1 HATCHERY 2 HATCHERY 3 HATCHERY 4 100% 83% 72% 73% Parameters Date Spray EQUIPMENT PERFORMANCE PROCESS AND ENVIRONMENT 61% 50% 59% 68% 81% 72% 57% 81% Area Number of hatchery Europe 4 Heterogeneous results All of the equipment controlled are performing badly 30 Vaccinology Summit Budapest

31 Spray : Equipment Set-up One disease, one nozzle, one pressure. Spray vaccination area needs to be protected from air drift. 4 Spray NOZZLE COLOUR VACCINATION PRESSURE (bars) TXVK6 Red IB / TRT* 4 TXVK8 Grey ND 5 TXVK18 Orange IB/ND in tropical areas Vaccinology Summit Budapest

32 Spray : Equipment Performance 4 Spray 32 Vaccinology Summit Budapest

33 Conclusions VACCINE PREPARATION Specific and exclusive area Asceptic conditions procedures 33 Vaccinology Summit Budapest

34 Conclusions EQUIPMENT Select the right equipment Well set and maintained Operator fully trained 34 Vaccinology Summit Budapest

35 Conclusions MONITORING Adapted to the vaccination program and equipment Regular monitoring of application and performance Data recording and data analysis 35 Vaccinology Summit Budapest

36 Conclusions Even the most effective vaccine worths nothing if it is not stored, prepared, and applied correctly The implementation of regular vaccination services, monitoring activities and continuous training is mandatory. 36 Vaccinology Summit Budapest

37 Thanks for your Attention 37 Vaccinology Summit Budapest