Fish Pathogen. qpcr Screening & ISO/IEC Accreditation Workshop. Tuesday, June 17, :30 am to 3:00 pm. Painter s Lodge. Campbell River, BC

Transcription

1 Fish Pathogen qpcr Screening & ISO/IEC Accreditation Workshop Tuesday, June 17, :30 am to 3:00 pm Painter s Lodge Campbell River, BC Hosted by the Centre for Aquatic Health Sciences

2 8:30-8:50 Registration 8:50-9:00 Opening Session 9:00-9:15 Ian Gardner (AVC-CERC-Charlottetown-PEI) OIE recommendations for aquatic animal health test validation 9:20-9:35 Kyle Garver (DFO-PBS-Nanaimo-BC) VHSv validation test 9:40-9:55 Maureen Purcell (USGS-Seattle-WA) IHNv validation test 10:00-10:15 Nellie Gagné (DFO-Moncton-NB) ISAv validation test 10:20-10:35 Café Break 10:40-10:55 Kim Klotins (CFIA-Ottawa-ON) NAAHP Network Laboratory Program requirements 11:00-11:15 Michael Sonntag (Ashbrooke QA-Port Coquitlam-BC) General process of ISO/IEC Laboratory accreditation 11:20-11:35 Tomy Joseph (Animal Health Centre, Abbotsford-BC) Animal Health Centre ISO/IEC accreditation 11:40-11:55 Mark Higgins (DFO-PBS-Nanaimo-BC) Fisheries and Oceans ISO/IEC accreditation 12:00-1:00 Lunch Break 1:00-1:20 Ahmed Siah/Debbie Collins Pathogen testing and ISO/IEC Laboratory accreditation Process (BC CAHS) 1:30 3:00 Discussion ISO/IEC accreditation and National Aquatic Animal Health Laboratory Network S CHEDULE

3 Ian Gardner Department of Health Management, University of PEI, Charlottetown, PEI Dr. Ian Gardner is the Canada Excellence Research Chair (CERC) in Aquatic Epidemiology at the University of Prince Edward Island (UPEI). Started in April 2011, the Canadian-government funded CERC program aims to make UPEI and Canada the global leader in applied aquatic epidemiology research with an ecosystem health focus. Prior to moving to Canada, Dr. Gardner was a Professor of Epidemiology at the University of California, Davis for 23 years. His current research interests include diagnostic test evaluation and implications of pathogen exchange between farmed and wild aquatic animals. Dr. Gardner obtained his veterinary degree at the University of Sydney in 1975 and completed post-graduate training (Master of Preventive Veterinary Medicine and PhD) at the University of California, Davis in Kyle Garver Aquatic Animal Health, Fisheries & Oceans Canada, Nanaimo, BC Kyle Garver is a Research Scientist with Fisheries and Oceans Canada, at the Pacific Biological Station in Nanaimo, BC where he heads the Virology Laboratory within the Aquatic Animal Health Section. Designated as the Canadian Reference Laboratory for viral hemorrhagic septicemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV), the PBS Virology laboratory conducts extensive diagnostic testing and investigates the epidemiology and disease ecology of these pathogens. Maureen Purcell Western Fisheries Research Center, U.S. Geological Survey, Seattle, WA Maureen is currently employed as a Research Microbiologist at the Western Fisheries Research Center US Geological Survey in Seattle, WA. Maureen received her B.S. in Zoology from Washington State University in 1993, her M.S. in Zoology from the University of Maine in 1997 and Ph.D. in Aquatic and Fishery Sciences from the University of Washington in Her current research focuses on a range of pathogens that infect salmonid and clupleid fishes, including Renibacterium salmoninarum, Icthyophonus sp., infectious hematopoietic necrosis virus and piscine orthoreovirus. In addition to research, she actively engages in training and technical outreach activities. Moderator : Ji m Powell (BC CAHS)

4 Nellie Gagné Centre for Aquatic Animal Health Research and Diagnostics (CAAHRD), Moncton, NB Nellie Gagné, scientist and head of the Moncton Molecular Biology group, has worked for the past thirteen years for DFO and established a molecular biology facility at DFO s Gulf Fisheries Center (GFC). The expertise of her group is in the development and validation of molecular diagnostics of fish diseases, and research focusing on fish health and disease control in general. A strong emphasis on quality is a trademark of the laboratory and insures that all steps of the projects will be managed in a rigorous manner. The GFC laboratory is the reference center for some pathogens, e.g. Infectious salmon anemia virus. Kim Klotins Domestic Disease Control Section, Canadian Food Inspection Agency (CFIA), Ottawa, ON Dr. Kim Klotins graduated from the Ontario Veterinary College in During her years of practice, Kim had the opportunity to work with the aquaculture and enhancement industries on the west coast, primarily with oysters, Pacific salmon and rainbow trout. Kim received her DVSc in Epidemiology from the Ontario Veterinary College in 2003 and worked with both the federal and Ontario government on issues such as salmonellosis, antimicrobial resistance and judicious use of antimicrobials. Kim started working for the Canadian Food Inspection Agency in 2006 on the National Aquatic Animal Health Program (NAAHP) as a risk assessor. Currently, she is National Veterinary Program Specialist for Domestic Control Programs for the NAAHP, responsible for programs involving receipt and response to notifications of regulated diseases and the domestic movement control program, which includes issuance of permits and compartmentalization. As part of those programs, the CFIA is working to establish a network laboratory system for the NAAHP. F ISH P ATH O GE N QPCR SCREENING & ISO/IEC A C C RED ITAT ION WO R KS H OP

5 Michael Sonntag Ashbrooke Quality Assurance, Port Coquitlam, BC Michael Sonntag has been a quality assurance professional since 1974 specializing in light and heavy engineering, construction and aerospace technologies and industries; service industries, including testing laboratories, telecommunications, metrology, and health services; and, professional service industries, including those specialized areas such as project management consultancies and accountancies. In addition to being Ashbrooke s Canadian President and International Group Managing Director, Michael is an IRCA Certified QMS Lead Auditor (IRCA Cert. No. A000532); and, author, creator and principal instructor for Ashbrooke's QMS / EMS / OHSMS auditor and Laboratory assessor training courses. Michael has been personally involved in leading and participating in hundreds of independent certification and supplier audits across several industrial and service disciplines worldwide. Michael has been personally involved in leading and participating in projects for the designing, developing and implementing of management systems towards satisfying the ISO 9001, ISO 14001, ISO / IEC 17025, OHSAS 18001, ISO Standards. Tomy Joseph Virology and Molecular Diagnostics, Animal Health Centre, Ministry of Agriculture, Abbotsford, BC Tomy Joseph joined the BC Ministry of Agriculture on August 1, 2013 as a Veterinary Virologist at the Animal Health Centre. For the past 6 years, Tomy was the Veterinary Virologist at the Veterinary Diagnostic Services of Manitoba Agriculture, Food and Rural Initiatives (MAFRI) in Winnipeg, Manitoba. Tomy graduated from the College of Veterinary and Animal Sciences, Kerala Agricultural University, India in1993. After spending 5 years in public veterinary practice, Tomy immigrated to Canada in 1998 to pursue graduate studies in veterinary and molecular virology. Tomy obtained an M.Sc. in 2001 and a Ph.D in 2004 for his thesis research on bovine herpesvirus-1 (BHV-1) and infectious salmon anemia virus (ISAV) respectively from the Atlantic Veterinary College, University of Prince Edward Island, PE, Canada. His thesis research contributed to the development of diagnostic techniques for BHV-1 and ISAV and understanding of ISAV pathogenesis. During his postdoctoral research from 2004 to 2007 at the Institute of Allergy and Infectious diseases, National Institutes of Health, Bethesda, Mary- F ISH P ATH O GE N QPCR SCREENING & ISO/IEC A C C RED ITAT ION WO R KS H OP

6 land, USA, Tomy developed live attenuated vaccines against avian influenza A H7 subtype viruses for human use and conducted preclinical evaluation in animal models. His research also focused on the development of diagnostic techniques for avian influenza viruses and the pathogenesis and immune response of avian influenza viruses in animal models. Tomy s areas of professional interest include development of diagnostic virology techniques, monitoring emergence of zoonotic viruses and foreign animal disease viruses and research in viral pathogenesis. Currently, Tomy collaborates with researchers from the Canadian Food Inspection Agency (CFIA) for the development of microarray based diagnostic tests and characterization of novel strains of avian and swine influenza viruses. Tomy holds an Asst. Professor appointment with Dept. of Medical Microbiology, Faculty of Medicine, University of Manitoba ( and sits on the advisory committees of graduate students as well. Tomy is also a technical assessor for the Standard Council of Canada for ISO accreditation audits. Mark Higgins National Aquatic Animal Health Program, Fisheries & Oceans Canada, Nanaimo, BC Mark has worked at the PBS for over 17 years in the Aquatic Animal Health group. He started working under Dr. Michael Kent on parasite issues affecting fish at hatcheries and spawning channels in BC. After the departure of Dr. Kent, he moved over to work with Dorothee Keiser on the Introductions and Transfers Committee and became the Local Fish Health Officer under the Fish Health Protection Regulations (FHPR) and eventually ended up moving into the National Aquatic Animal Health Program (NAAHP) as the QA Manager and then the NAAHP Program Head. Mark still participate on the I&T Committee and works as the NAAHP program head for the Pacific Region along with Section head duties for the Aquatic Animal Health section. Ahmed Siah BC Centre for Aquatic Health Sciences, Campbell River, BC Ahmed joined the team at BC CAHS in November 2011 as the Research Scientist/ Microbiology Diagnostician. His duties include managing research projects, developing new technologies in the field of aquatic health diagnostics and implementing and F ISH P ATH O GE N QPCR SCREENING & ISO/IEC A C C RED ITAT ION WO R KS H OP

7 validating molecular biology technologies for diagnostics. Dr. Siah pursued his Postdoctoral studies in Molecular Ecotoxicology at the University of Le Havre in France after earning his PhD in Oceanography at the Institute of Marine Sciences in Rimouski, Quebec. As a Research Associate at Prince Edward Island's Atlantic Veterinary College, he led and managed several projects on mollusk health management and the development of molecular diagnostic tools. Ahmed's research focused primarily on identifying molecular biomarkers related to shellfish diseases and on developing new technologies to detect micro-organisms affecting fish and shellfish health. While at the Atlantic Veterinary College, Ahmed worked on hemic neoplasia in soft shell clams and developed diagnostic assays for rapid and high-throughput detection of tunicate invasive species which are heavily affecting the mussel industry in PEI. Debbie Collins BC Centre for Aquatic Health Sciences, Campbell River, BC Debbie worked at a health research facility for twenty-four years before moving to Campbell River in Debbie has moved her focus from human to aquatic fish health and presently is a Laboratory Technician at CAHS. She joined the Centre in 2005 and her main role is lab services such as ATPase and Cortisols. She also purchases all new equipment and lab consumables. Debbie is involved with the implementation of the Laboratory Information Management System (LIMS) at CAHS. Jim Powell BC Centre for Aquatic Health Sciences, Campbell River, BC Dr. Jim Powell has over 30 years experience in the areas of fisheries and aquaculture sciences and is an established authority on fish reproduction and broodstock management both locally and internationally. His primary work has been in the area of fish physiology and adaptive management strategies of fish culture for a range of freshand salt-water species. He has extensive experience in aquaculture drug development, testing, registration and implementation in addition to work in fish health. Most recently, Jim was involved in fisheries and aquaculture management in the recreational and conservation sector with involvement in threatened and endangered species recovery. Dr. Powell is a founding Board member of the BC CAHS and has served on the board since inception. F ISH P ATH O GE N QPCR SCREENING & ISO/IEC A C C RED ITAT ION WO R KS H OP

8 ABOUT BC CAHS BC CAHS is a not-for-profit society located in Campbell River and is the only aquatic research facility of its kind in the province. Currently, BC CAHS employs 11 people. MISSION BC CAHS exists to improve the health of aquatic animals and ecosystems, by developing applied scientific knowledge. VISION Delivering innovative and practical solutions for aquatic animal and environmental health.