C H R I ST I N A M. LO I ACONO DV M, P H D, D I P. ACVP
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1 AQUATIC PATHOGEN TESTING IN NAHLN LABORATORIES UPDATE C H R I ST I N A M. LO I ACONO DV M, P H D, D I P. ACVP N A H L N A S S O C I AT E COORDINATO R U. S. D E PA R T M EN T O F AGRICULT URE A N I M A L A N D PLANT H EALT H I N S P EC T I O N S E RV I C E V E T E R I N A RY S E RV I C ES N AT I O N A L A N I M A L H EALT H L A B O R ATO RY N E T WORK STA S O C TO B ER
2 National Animal Health Laboratory Network Formed in 2002 Purpose Early detection Rapid response Appropriate recovery Is a partnership between: USDA (APHIS and NIFA) AAVLD NAHLN Laboratories 2
3 Founding Principles and Features of NAHLN Quality standards Competency of laboratory personnel Standardized protocols and equipment Adequate biosafety/biosecurity Secure electronic communications and reporting Assessment of preparedness through scenario testing 3
4 NAHLN Laboratory Qualification Checklist For Membership of a Veterinary Diagnostic Laboratory Annual renewal Agree to meet requirements of the NAHLN Quality Management Foreign Animal Disease (FAD) Assays and Investigations Sample Handling Communication and Reporting Administrative and Financial Requirements Request any changes to disease/agent approvals Signatures needed from State (SAHO, etc.) and Federal representative (DD or AD)
5 NAHLN restructure Level 1 Large testing capacity Fully accredited BSL3 facilitates LIMS/messaging Trainers Test development and validation Level 2 Similar, but reduced capacity Provisionally accredited No BSL 3 requirements Level 3 Surveillance testing Affiliate Labs Publically funded that occasionally perform NAHLN related-testing Private Labs Specific, needed capability to perform testing Relationship with NAHLN lab and SAHO Written, approved plan to avoid conflicts of interest Reference Labs provide Oversight Training SOPs Reference material Proficiency testing 5
6 Pullman National Animal Health Laboratory Network (NAHLN) Puyallup Corvallis Bozeman Fargo St. Paul Davis Logan Tucson NAHLN Approved Laboratories *Bovine Spongiform Encephalopathy (BSE) Newcastle Disease (ND)/Influenza A Virus in Avian (IAV-A) **Scrapie/Chronic Wasting Disease (CWD) *Classical Swine Fever (CSF)/Foot and Mouth Disease (FMD) *Pseudorabies Virus (PRV) Influenza A Virus in Swine (IAV-S) Vesicular Stomatitis (VS) African Swine Fever Laramie National Veterinary Services Laboratories Fort Collins Rocky Ford Albuquerque Brookings Amarillo Lincoln Manhattan Austin Ft. Sam Houston Stillwater Ames College Station Gonzales Viral hemorrhagic septicemia (VHS) Infectious salmon anemia (ISA) SVC Approved Lab* Center Columbia Madison Galesburg Springfield Little Rock Lansing West Reynoldsburg Urbana Lafayette Moorefield Lexington Harrisonburg Stoneville Auburn Baton Rouge Pearl Hopkinsville University Park Harrisburg Nashville Gainesville Athens Kennett Square Tifton Raleigh Columbia Ithaca Kissimmee July 20, 2016 Storrs Plum Island Ewing Georgetown Newark Frederick Salisbury *For specified agents, not all laboratories are currently participating in surveillance testing **Multiple assays conducted for this agent, refer to specific disease map/list for assay options.. Pearl City
7 NAHLN Laboratories Approved to Conduct Aquatic Pathogen Surveillance Testing Pullman Corvallis Brookings St. Paul Storrs Davis Ames Madison Ewing Reynoldsburg West Lafayette Manhattan Stoneville ISA Approved Lab* VHS Approved Lab* SVC Approved Lab* NVSL Lab Site * Pending successful completion of the PT Kissimmee September 27, 2016
8 8
9 Testing numbers ISAV PT (RT- real time PCR) Total of 1100 PCR tests completed in FY2016 One lab reported VHSV PT (VI or RT- real time PCR) Total of 134 PCR tests completed in FY2016 Total of 6989 VI completed in FY2016 Five labs testing
10 Upcoming PTs ISAV PT (RT- real time PCR) 9 labs eligible to take the PT VHSV PT (VI or RT- real time PCR) 13 labs eligible to take the PT SVC PT (VI) 6 labs eligible to take the PT 10
11 Next steps Complete proficiency testing for three aquatic pathogens under NAHLN scope NAHLN program staff will work with Aquaculture commodity staff and Reference Lab staff to determine if and when more aquatic pathogens should be added to NAHLN scope Continue to offer Quality Management System training that members of current and prospective NAHLN labs are welcome to attend 11
12 Questions?
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