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1 RQS PT PT Scheme [Jul Aug] & [October- November] PLEASE NOTE: RQS Pharma [Microbiology Chemistry] Testing PT Scheme [Jul Aug] & [October- November] - Assessment No.PH-03 & PH-04 (1) All Pharma PT schemes include samples which are either Material, which contain levels of contaminants usually, found in Pharma samples. (2) Some Pharma PT schemes (PH-MB) May be DNA /RNA Extract of culture (CRM). (3) Assigned value, Homogeneity and Stability performed by Subcontractor Lab. 1. PT ode Matrix Analyte Approx. Size PH-MB-4418 PT Sample For Sterility Test by using Culture methods 2. W-MB-4518 PT Sample For Enumeration of Aerobic Bacterial plate count By using Culture technique 3. PH-MB-4618 PT Sample For Detection of Bacterial Endotoxin [ Limulus amebocyte lysate Test by using (LAL Test). 4. PH-MB-4718 [Referen ce Material PT Sample For determination of Potency of Gentamycin sulfate By using Biological Assay 5. PH-MB-4818 Detection and Enumeration of Staph auras. 6. PH-MB-4918 Detection and Enumeration of Staph aureus. F-RQS-05 PT SCHEM Page 1 of 6

2 PT ode Matrix Analyte Approx. Size 7. PH-MB-5018 PT Sample For Enumeration of Yeast and Mould by using Culture technique PH-CH-5118 Material PT Sample For determination of Water content by Karl Fischer PH-CH-5218 CRM PT Sample For determination of ph 50 ml 10. PH-CH-5318 PT Sample For determination of Viscosity 50 ml 11. PH-CH-5418 Material PT Sample For determination of Water content by Karl Fischer PH-CH PH-CH-5518 Material PT Sample For determination of heavy Metal by Atomic absorption PH-CH Determination of Conductivity PH-CH Refractive index PH-CH Optical rotation] 16. PH-CH-5918 [Determination moisture by loss weight] F-RQS-05 PT SCHEM Page 2 of 6

3 PT ode Matrix Analyte Approx. Size 17. PH-MB-6018 PT Sample for Assay for virus content by using egg inoculation [Allantoic cavity] for egg adapted (ND) viral vaccine 18. PH-MB-6118 PT Sample for Assay for virus content by using egg inoculation on [Chorio allantoic membran] for egg adapted Fowl Pox [POX] vaccine, 19. PH-MB-6218 [Serum] PT Sample for determination titer of FMD antibody by using S.N.T 200 µl 20. PH-MB-6318 PT Sample for determination titer of Rift valley fever antibody by using S.N.T 100 µl 21. PH-MB-6418 PT Sample for detection of hemagglutinating agents in attenuated poultry viral vaccines 22. PH-MB-6518 PT Sample for determination of virus content of sheep box vaccine by using tissue culture titeration technique 23. W-MB-6618 Serum PT Sample for Differentiate between vaccine and infected sample for FMD by using ELISA. 200 µl 24. W-MB-6718 Serum PT Sample for detection of Ab of avian influenza by using ELISA 1 ml 25. PH-MB-6818 Equine Influenza by using ELISA. 26. PH-MB-6918 West Vile fever by using ELISA. F-RQS-05 PT SCHEM Page 3 of 6

4 PT ode Matrix Analyte Approx. Size 27. PH-MB-7018 Vesicular Stomatitis by using ELISA. 28. PH-MB-7118 RVF IgM 29. PH-MB-7218 RVF IgG 30. PH-MB-7318 PT Sample for Detection of bovine viral diarrhea (BVD) virus genotypes by using polymerase chain reaction (PCR) 31. PH-MB-7418 PT Sample for assay for AB titeration for the following AI,ND & EDS by using HI Test 32. PH-MB-7518 PT Sample for assay for detection of antibodies by using [ELISA] against the following diseases : IB,IBD 33. PH-MB-7618 PT Sample for Detection of Lecosis by ELISA in Vaccine PH-MB-7718 PH-MB-7818 PT Sample for Detection of Lecosis by ELISA in Albumen PT Sample for Detection of Antibody against Gumboro by agar gel precipitation test in serum F-RQS-05 PT SCHEM Page 4 of 6

5 PT ode Matrix Analyte Approx. Size 36. PH-MB-7918 PT Sample for Detection of Antibody against gumboro by using newtraliztion test in serum 37. PH-MB-8018 PT Sample for Detection of REV antigen by using PCR PH-MB-8118 PH-MB-8218 [DNA Bacterial PT Sample for Assay of virus content by using tissue culture for IBD (tissue culture) adapted poultry viral vaccines PT Sample for Assay DNA Extract for detection of the following: Brucella abortus biovar 1[S19],Brucella abortus biovar 1[RB51], Brucella melitensis biovar 1 (Rev-1) 100µL 40. PH-MB-8318 [DNA Virus PT Sample for Assay DNA virus extract by using PCR against the following : Lumpy skin disease (LSD), Sheep Pox, Infectious bovine Tracheitis (IBR), Equine herpes virus. 50 µl 41. W-MB-8418 [RNA Virus PT Sample for Assay RNA virus extract by using PCR against the following : Rift valley fever (RVF), Blue Tongue virus., Rabies virus, West Nile fever virus, Foot and mouth disease virus., African horse sickness virus., Equine Influenza virus. 50 µl 42. W-MB-8518 Culture PT Sample For Serotyping of Salmonella F-RQS-05 PT SCHEM Page 5 of 6

6 PT ode Matrix Analyte Approx. Size 43. W-MB-8618 [ Culture] PT Sample for Detection of Salmonella by using Real time RT PCR 44. PH-MB-8718 PH-MB-8818 W-MB-8918 [DNA Bacterial Culture PT Sample for Assay of virus content by using tissue culture for IBD (tissue culture) adapted poultry viral vaccines PT Sample for Assay DNA Extract for detection of the following: Brucella abortus biovar 1[S19],Brucella abortus biovar 1[RB51], Brucella melitensis biovar 1 (Rev-1) PT Sample For Serotyping of Salmonella PH-MB-9017 Serum PT Sample for assay for detection of antibodies by using [ELISA] against the following diseases : IB,IBD and TRT PH-MB-9117 DNA Extract PT sample for Detection of tuberculosis [TB] by using PCR PH-MB-9217 Serum PT Sample for assay for detection of the following By using ELISA Technique Mycoplasma Gallisepticum,Mycoplasma Synoviae.Mycoplasma Mycoides [Contagious Bovine Pleuro Pneumonia] [PPLO],Salmonella spp.,e.coli PPlo MG MG Sample DSPF PH-MB-9217 CRM Determination of Sodium Benzoate By using HPLC 100µL fouadeltahan@yahoo.co.uk Rqs_pt@yahoo.com F-RQS-05 PT SCHEM Page 6 of 6

F-RQS-05 PT SCHEM Page 1 of 5

F-RQS-05 PT SCHEM Page 1 of 5 RQS PT PT Scheme 2018 [Jul-Aug] [October-November] PLEASE NOTE: RQS [Microbiology Chemistry] Testing PT Scheme 2018- [Jul- Aug] [October-November] - Assessment No.F-03 & F-04 (1) All food PT schemes include

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