Státní veterinární ústav Praha Zkušební laboratoř diagnostiky infekčních nemocí zvířat Sídlištní 136/24, Praha 6 Lysolaje

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1 Page 1 of 7 The workplace of the testing laboratory: 1 Sídlištní 136/24, Praha 6 Lysolaje Tests: a. 6b. Detection of antibodies against Brucella spp. by the method Rose Bengal test (Institut Pourquier), the diagnostic kit for slow agglutination and complement fixation reaction (Bioveta), ELISA Chekit Brucella abortus Antibody test/bovine milk test kit (Idexx), Pourquier ELISA brucelosis serum-s test kit (Institut Pourquier) Detection of antibodies against Trypanosoma equiperdum by the complement fixation reaction kit for exanthema coitale vesiculosum paralyticum (Bioveta) Detection of antibodies against Burkholderia mallei by complement fixation reaction kit for the detection of dourine (Bioveta) Detection of antibodies against Mycobacterium paratuberculosis by the complement fixation reaction kit (CIDC- Lelystad), ELISA ID Screen Paratuberculosis indirect test kit (ID Vet) and Pourquier ELISA Paratuberculosis test kit (Institut Pourquier) Detection of antibodies against Leptospira spp. by microagglutination test Detection of foot-and-mouth disease virus by virus isolation in cell cultures, by antigen ELISA (WRL Pirbright) and by molecular methods (real time RT-PCR) Detection of antibodies against foot-andmouth disease virus by virus neutralisation test, LPB ELISA (WRL Pirbright), Priocheck FMD NSP ELISA (Prionics) and Priocheck FMD Type O (Prionics) SOP 201 SOP 202 SOP 203 SOP 204 SOP 205 (O.I.E., Chap ) SOP 301a (O.I.E., Chap ) SOP 301b (O.I.E., Chap ), milk, tissue, swab

2 Page 2 of 7 7a. 7b. 8. 9a. 9b. 10a. 10b. 11a. 11b. 12. Detection of swine vesicular disease virus by virus isolation in cell cultures and antigen ELISA (WRL Pirbright) Detection of antibodies against swine vesicular disease virus by ELISA Pricheck SVDV Ab test kit (Prionics) and virus neutralisation test Detection of vesicular stomatitis by virus isolation in cell cultures and virus neutralisation test Detection of Newcastle disease virus by virus isolation in chicken embryos, hemagglutination test, pathogenicity determination of APMV1, molecular methods (real time RT-PCR) and intracerebral pathogenicity index Detection of antibodies against Newcastle disease virus by hemagglutinationinhibition test Detection of avian influenza virus by virus isolation in chicken embryos, hemagglutination test, intravenous pathogenicity index determination and molecular methods (real time RT-PCR on matrix protein, H5 and H7 subtype determination) Detection of antibodies against avian influenza virus by immunodiffusion test, ELISA Idexx Influenza A Ab Test kit (Idexx) and hemagglutination-inhibition test Detection of swine fever virus by virus isolation in cell cultures Detection of antibodies against swine fever virus by ELISA Priocheck CSFV test kit (Prionics) and Herdchek CSFV Ab test kit (Idexx) Detection of antibodies against enzootic bovine leucosis virus by immunodiffusion test (Institut Pourquier), ELISA test kit (Test-line) and Pourquier ELISA Bovine Leucosis test kit (Institut Pourquier) SOP 302a (O.I.E., Chap ) SOP 302b (O.I.E., Chap ) SOP 303 (O.I.E., Chap ) SOP 304a (O.I.E., Chap ) SOP 304b (O.I.E., Chap ) SOP 305a (O.I.E., Chap ) SOP 305b (O.I.E., Chap ) SOP 306a (O.I.E., Chap ) SOP 306b SOP 307, tissue, tissue, feces, swab, feces, swab, milk

3 Page 3 of a. 15b a. 18b Detection of Aujeszky s disease by ELISA ID Screen Aujeszky gb competition test kit (ID Vet) Detection of infectious bovine rhinotracheitis by BHV-1 ELISA test kit (Test-line), Svanovir IBR Ab Test kit (Svanova), Idexx IBR Ab ge test kit (Idexx) and Idexx IBR Ab gb test kit (Idexx) Detection of bovine viral diarrhoea virus by Herdchek ELISA BVDV P80 Antigen Test kit (Idexx) and molecular methods (real time RT-PCR) Detection of antibodies against bovine viral diarrhoea virus by ELISA BVD/MD/BD P80 Ab Screening test kit (Institut Pourquier) Detection of antibodies against PRRS virus by Herdchek PRRS X3 test kit (Idexx) Detection of antibodies against equine infectious anemia virus by immunodiffusion test (VMRD and Idexx) Detection of equine arteritis virus by virus isolation in cell cultures and molecular methods (real time RT-PCR) Detection of antibodies against equine viral arteritis by virus neutralisation test Detection of antibodies against poultry infectious bronchitis by Infectious Bronchitis Virus Antibody Test kit FlockCheck (Idexx) Detection of antibodies against infectious bursitis virus by Infectious Bursal Disease Virus Antibody Test kit FlockCheck (Idexx) SOP 308 SOP 309 SOP 310 SOP 310 SOP 311 SOP 312 SOP 313a (O.I.E., Chap ) SOP 313b (O.I.E., Chap ) SOP 314 SOP 315, tissue, ejaculate

4 Page 4 of Detection of antibodies against Maedi Visna virus by Maeditect 100 Test kit (VLA, Weybridge), Pourquier ELISA Maedi-Visna/CAEV screening test kit (Institut Pourquier) and Pourquier ELISA Maedi-Visna/CAEV Verification test kit (Institut Pourquier) Detection of rabies virus by direct immunofluorescence and biological test Detection of mycobacteria by microscopy, culture and molecular methods (PCR, gene probe) Detection of Taylorella equigenitalis by Detection of Salmonella spp. by culture and fast agglutination Detection of Campylobacter fetus by Detection of Francisella tularensis by Detection of Brucella melitensis biovars (Abortus, Suis, Ovis, Canis, Melitensis, Neotomae) by culture and molecular methods (PCR) SOP 316 SOP 401 (O.I.E., Chap ) SOP 501 (O.I.E., Chap , , ) SOP 502 (O.I.E., Chap ) SOP 503 (O.I.E., Chap ) (ČSN EN ISO 6579) SOP 504 (O.I.E., Chap ) SOP 505 (O.I.E., Chap ) SOP 506 Detection of Pasteurella spp. by culture SOP 507 Detection of Paenibacillus larvae subsp. larvae by culture and molecular methods (PCR) Detection of Melissococcus pluton by Detection of Listeria spp. by culture and molecular methods (PCR, gene probe) (O.I.E., Chap , , , ) (MCM) SOP 508 (O.I.E., Chap ) SOP 509 (O.I.E., Chap ) SOP 510 (ČSN EN ISO , ČSN EN ISO 10560) CNS tissue, feces, feedstuffs, samples of the environment Preputial lavage, swab, tissue, ejaculate, feces, smears, meconium, eggs, samples of breeding environment, bacterial culture Preputial lavage, swab, tissue, ejaculate Swab, tissue Swab, tissue Swab, tissue Honey combs, pulp, beeswax, bacterial culture Honey combs, pulp, beeswax, bacterial culture, milk, bacterial culture

5 Page 5 of a. 38b Identification of bacteria (Helicobacter spp., Campylobacter spp., Mycoplasma spp., Pasteurella spp., Streptococcus spp., Staphylococcus spp., Citrobacter rodentium, Streptobacillus moniliformis, Corynebacterium kutscheri, Yersinia spp., Clostridium spp., Salmonella spp., Bordetella bronchiseptica) isolated from laboratory animals by culture methods Testing of bacteria antimicrobial susceptibility by disk diffusion method Testing of bacteria antimicrobial susceptibility by microtitration dilution method for the determination of minimum inhibitory concentration Determination of antibodies against rabies virus by virus neutralization test Detection of trichinelosis by digestive method Determination of antibodies against ovine catharal fever (bluetongue) virus by ID Screen Bluetongue Competition test kit (ID Vet) Detection of bluetongue virus by Real-Time RT-PCR Detection of tetracycline biomarker in bone fractions Detection of IgG antibodies against rabies virus glycoprotein ELISA Platelia Rabies II Detection of varroa mite (Varroa destructor) by flotation method SOP 511 (HLAB) SOP 512 CLSI M31-A3(M100- S21, M02-A10) SOP 513 CLSI M31-A3 (M100- S2, M02-A10) SOP 402 (O.I.E., Chap ) SOP 403 (O.I.E., Chap ) SOP 317 SOP 318 (O.I.E., Chap ) SOP 404 (O.I.E., Chap ) SOP 405 SOP 406 (O.I.E., Chap ) Rabies virus titration - Determination of SOP 407 TCID 50 (O.I.E., Chap ) Primary cultures of bacteria from tissues of laboratory animals Bacterial culture Bacterial culture Blood, blood Bee pulp, adult bees, drone brood Vaccination dose

6 Page 6 of Detection of Bacillus antracis by microscopy, culture and molecular methods (PCR) SOP 514 (O.I.E., Chap ), blood, tissue, samples of the environment 44. Universal taxonomic identification system of bacteria and lower fungi by MicroSeq method SOP Lower fungi culture, bacterial culture, smears 45. Detection of West Nile Fever by ID Screen West Nile Competition ELISA test kit (ID Vet) SOP Detection of West Nile Fever by real time RT-PCR method SOP 320 Blood, swab, tissue 47. Detection of antibodies against Coxiella burnetii (Q fever) by complement fixation reaction (Virion/Serion) and ELISA ID Screen Q fever indirect Multi-species test kit (ID Vet) SOP 321, blood 48. Identification of bacteria and lower fungi by MALDI-TOF method SOP 515 (Manufacturer's manual, Bruker Daltonics) Bacterial culture 49. Molecular identification of species specific DNA from tissues and their products SOP s, meat products, feedstuffs 50. Pathomorphological examination of vertebrates SOP Detection of bone fractions by alizarin red staining method SOP 409 (Codex Alimentarius Austriaticus, Teilkapitel D4) 1) s, meat products

7 Page 7 of 7 The workplace of the testing laboratory: 2 Laboratoř diagnostiky Trichinella spp. Jateční 316, Český Brod 1) 37. Test name Detection of trichinellosis by digestion method Method identification SOP 403 (O.I.E., Chap ) Tested object Asterisk at the ordinal number identifies the tests carried out outside the laboratory premises. O.I.E., Chap. Standard Operating Procedure prepared according to the relevant numbered chapter of the manual of standard methods Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 6 th, Ed: Office International des Epizooties (O.I.E.), Paris, 2008, ISBN MCM - Standard Operating Procedure prepared according to the relevant chapter of the Manual of Clinical Microbiology10 th edition, Ed: ASM, 2011, ISBN , page 425. HLAB - Standard Operating Procedure prepared according to the Handbook referred to - Hansen A.K. : Handbook of Laboratory Animal Bacteriology, Ed: CRC Press LLC, 2000, ISBN CLSI M31-A3-Standard Operating Procedure prepared according to the methods specified in the Manual of Standards of the Clinical and Laboratory Standards Institute, 2008, Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - Third Edition ISBN X CLSI M100-S21-Standard Operating Procedure prepared according to the methods specified in the Manual of Standards of the Clinical and Laboratory Standards Institute, 2011, Performance Standards for Antimicrobial Susceptibility Testing; Twenty- First International Supplement ISBN CLSI M02-A10-Standard Operating Procedure prepared according to the methods specified in the Manual of Standards of the Clinical and Laboratory Standards Institute, 2009,Performance Standards for Antimicrobial Disk Susceptibility Testing Approved Standard - Tenth Edition. 2009, ISBN IBR Infectious Bovine Rhinotracheitis PRRS Porcine Reproductive and Respiratory Syndrome IgG Immunoglobulin G TCID 50 50% infectious dose for tissue and cell cultures measured by cythopathic effect evaluation MALDI-TOF - MALDI (Matrix Assisted Laser Desorption/Ionization) type mass spectrometry with ion source and vertically aligned TOF (time-of-flight) analyzer. The method is designed for the automatic identification and characterization of proteins, biomarker detection and qualitative oligonucleotide control.