Supplementary Information

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1 Electronic Supplementary Material (ESI) for Molecular BioSystems. This journal is The Royal Society of Chemistry 2014 Supplementary Information Supplementary table S1- Hu-PPI and Hu-Vir PPI data before and after the removal of ambiguous and redundant protein identifiers (those with identities >98% and ±20 of protein length in BLAST searches). * strains not merged and ** strains merged. Identity cut off DATA Interactions Interactions after Proteins in BLAST before BLAST BLAST (no. of proteins involved) 98% Hu-PPI % (human proteins) 98% (viral proteins) 30% (viral proteins) (10658) Hu-Vir PPI (1754 human +763 viral) Hu-Vir PPI (1735 human viral) Hu-Vir PPI (1735 human +763 viral) Human 763 Viral 9732* 1735 Human 518 Viral 3389** 1735 Human 267** Viral

2 Supplementary table S2 - Salient features of viruses used in the study. This table gives number of proteins encoded by virus, number of Hu-Vir PPI for that virus, virus host or disease caused, site and type of infection caused by virus and virus classification. Virus No. of ORFs or protein encode by virus No. of Hu- Vir PPI Virus host /disease Site and type of infection Classification Adeno associated virus 2 Autographa californica nuclear polyhedrosis virus 7 2 no known disease found in semen ssdna, Parvoviridae insect virus coelum Baculoviridae Avian sarcoma virus NA 2 Bird lymphoma and mammals connective tissue, lymphatic tissue Bovine herpes virus cattle rhinotracheitis, vaginitis, conjunctivitis Bovine leukemia virus 6 7 cattle B-cell leukemia Bovine papilloma virus 1 Bovine papilloma virus cattle skin, bladder cancer 9 1 cattle skin, bladder cancer Bovine Rotavirus NA 1 cattle diarrhoea intestinal tract dsrna, Reoviridae Chicken anemia virus 4 1 birds bone marrow atrophy ssdna, Circoviridae Cotton tail rabbit papilloma virus 10 5 rabbit skin, bladder cancer Dengue virus humans, dengue fever Epstein barr virus humans, cancer, Burkitt's lymphoma hemorrhagic, fever, diarrhoea B-cells and epithelial cells (+)ssrna, Flaviviridae Equine herpes virus 1 NA 1 horse, pneumonia pneumonia, paralysis Equine herpes virus 4 NA 2 horse conjuctivitis, swollen lymph nodes Equine infectious anemia virus 4 1 horse, anemia and fever break down of blood cells Foamy virus NA 1 humans, simians, lymhoblastoid, carcinoma

3 camcer Fowl adenovirus A 39 2 fowl, infection respiratory Hantaan virus 1 4 human, hemorrhagic fever blood vessels, kidney, lungs, liver Bunyaviridae Hepatitis B virus 7 27 humans, hepatitis B liver and pancreas cancer, cirrhosis dsdna-rt, Hepadnaviridae Hepatitis C virus 2 91 humans, hepatitis C hepatocytes (+)ssrna, Flaviviridae Hepatitis D virus 2 2 humans, alongwith hepatitis B liver and pancreas cancer, cirrhosis unassigned Hepatitis E virus 10 2 humans, hepatitis E liver (+)ssrna, Hepeviridae Human adenovirus A human, infection respiratory Human adenovirus B 38 4 human, infection respiratory Human adenovirus C human, infection respiratory Human adenovirus D 38 1 human, infection respiratory Human adenovirus E 38 3 human, infection respiratory Human adenovirus F 34 1 human, infection respiratory Human entero virus B, Echovirus 1 1 humans, menigitis brain, meingitis (+)ssrna, Picornaviridae Human herpes virus humans, chickenpox and shingles Human herpes virus humans, oral and genital herpes Human herpes virus humans, oral and genital herpes mucoepithelial, skin and nerve cells mucoepithelial and nerve cells mucoepithelial and nerve cells Human herpes virus 5(cytomegalovirus) humans, infectious mononucleosis-like syndrome Monocyte, lymphocyte, and epithelial cells Human herpes virus humans, sixth disease or rose rash disease T-cells

4 Human immunodeficiency virus humans, AIDS(global) T-cells SsRNA-RT, Human immunodeficiency virus humans, AIDS(west Africa) T-cells Human Papilloma virus High risk humans, cervical cancer and warts keratinocytes and epithelial cells Human papilloma virus type Low Risk 9 53 humans, cervical cancer and warts keratinocytes and epithelial cells Human T-lymphocytic virus humans, HTLV myelopathy, leukemia T-cells, B-cells and dendritic cells Human T-lymphocytic virus humans, HTLV myelopathy T-cells, B-cells and dendritic cells Influenza A virus H1N1 Influenza A virus H3N2 Influenza A virus H7N humans, Influenza bronchial epithelial cells Orthomyxoviridae 9 2 humans, Influenza bronchial epithelial cells Orthomyxoviridae 9 1 humans, Influenza bronchial epithelial cells Orthomyxoviridae JC polyomavirus 6 3 humans, progressive multifocal leukoencephalopath y tonsils, gastrointestinal tract, nervous tissue Polyomaviridae Kaposi sarcoma associated herpes virus 2 10 humans, kaposi sarcoma Lymphocyte Measles virus 7 6 humans, measles respiratory tract Paramyxoviridae Mokola virus 5 1 humans and animals, encephalitis Murine adenovirus 26 1 mice, acute CNS disease nervous system hemorrhage in brain and spinal cord Rhabdoviridae Murine herpes virus mice, infection nasal passages Murine polyoma virus 6 5 mice, leukemia gangliosides Polyomaviridae Nipah virus 8 1 humans and mammals, infection respiratory and nervous system Paramyxoviridae

5 Orf virus sheep and humans, sheep pox skin Poxvidae Papiine herpes virus 75 4 baboon, infection neuro-virulent Para influenza virus 9 2 humans, Croup respiratory tract Paramyxoviridae Polio virus 1 2 humans, poliomyelitis Puumala virus 1 2 human, hemorrhagic fever Rabies virus 5 1 humans and dogs, Rabies nervous system hemorrhagic fever with renal syndrome Nervous system (+)ssrna, Picornaviridae Bunyaviridae Rhabdoviridae Rous sarcoma virus 4 9 chicken, sarcoma connective tissue Saimiriine herpesvirus sqirrel monkeys, humans in laboratory skin and epithelial cells SARS 14 4 humans, SARS respiratory tract (+)ssrna, Coronaviridae Sendai virus 9 1 mammals, infection respiratory tract and lungs Paramyxoviridae Seoul virus NA 3 humans hemorrhagic fever Bunyaviridae Simian immunodeficiency virus 7 2 primates, related to HIV T-cell Simian rotavirus NA 2 primates, diarrhoea gut and intestine dsrna, Reoviridae Simian virus primates, latent infection Stomatitis virus 5 2 mammals(infection ), humans(oncolytic) vacuolating cytolytic Polyomaviridae Rhabdoviridae Tula virus 1 1 humans, fever NA Bunyaviridae Vaccinia virus humans, smallpox vaccine West nile virus 1 1 mammals, meningitis and encephalitis skin nervous system and lymphatic tisse Poxvidae (+)ssrna, Flaviviridae Zaire ebolavirus 9 1 humans, endothelial lining and

6 hemorrhage blood vessels Filoviridae Supplementary Figure S1- Venn diagram showing distribution of human proteins in Hu-PPI and Hu-Vir PPI data Supplementary Figure S2 Gene Ontology (GO) molecular function enrichment for peripheral proteins in Hu-PPI network (a) and 176 human proteins interacting with viruses but not part of Hu-PPI network (b).

7 Supplementary Figure S3 Gene Ontology (GO) biological process enrichment for peripheral proteins in Hu-PPI network (a) and 176 human proteins interacting with viruses but not part of Hu-PPI network(b).

8 Supplementary Figure S4 Gene Ontology (GO) cellular component enrichment for peripheral proteins in Hu-PPI network (a) and 176 human proteins interacting with viruses but not part of Hu-PPI network (b).