Exeter Clinical and Health Research

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Clarence Hawkins
5 years ago
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1 Exeter Clinical and Health Research Standard Operating Procedure: 10 Second Wingate Test SOP Number: NIHRexe255REJUVENATE Version Number & Date: V1.0 22/12/2016 Review Date: 22/06/2017 Superseded Version Number & Date: V (if applicable) / / Author: Name: Colleen Deane Position: Research Assistant Approved by: Name: Timothy Etheridge Position: Chief Investigator Senior Management Agreement: Name: Gillian Baker Role: CRF Manager I agree that appropriate members of my workforce (as named above) have written and approved this SOP for use in clinical research. Page 1 of 5

2 Page 2 of 5 1. BACKGROUND: The Wingate test is a maximal effort cycling sprint exercise test used to measure the thigh muscles maximal power producing ability. The test has been used safely in multiple populations including athletes (Nieman et al., 2013) and older adults (Marsh, Paterson, Govindasamy, & Cunningham, 1999) to provide repeatable measures of anaerobic capacity. 2. SCOPE: This SOP applies generically to clinical trials and research projects, clinical and health research in Exeter, unless a trial agreement specifically indicates that another organisations SOP should be used. 3. PURPOSE: The purpose of this SOP is to ensure correct and uniform administration of the Wingate protocol in the CRF. 4. DEFINITIONS AND ABBREVIATIONS CRF Clinical research facility ECG electrocardiogram kg kilogram g gram PC personal computer RPM revolutions per minute ID identification VGA video graphics array 5. ROLES AND RESPONSIBILITIES: It is the responsibility of staff undertaking clinical research to read and use this SOP when administering a Wingate test. It is the role of the clinical doctor to fit a 12-lead ECG to participants. To account for the possibility of adverse events arising from the maximal exertion exercise a clinical doctor must be present when conducting this test. 6. SKILL LEVEL: This procedure should only be carried out by personnel who have undergone the appropriate training. Only clinical doctors should attach ECG leads to the participants and monitor and interpret ECG traces. 7. EQUIPMENT: Monark cycle ergometer, Ergomedic 894 E PC with Monark Anaerobic test software Monark power cable Appropriate cable (VGA to PC) to connect the ergometer to the PC Assorted disc weights: 5 x 1 kg, 4 x 500 g, 10 x 100 g.

3 ECG equipment (probably need to be specific here get model from lab) Drinking water If volunteers feel faint lay them on the bed (in the exercise room where the test is performed). 8. PROCEDURE: Pre-test set-up: Connect the VGA cable to the ergometer and to the USB port on the PC Connect the power adapter into a plug socket on the wall and into the bike Press the switch, located on the bike near the VGA connection point Turn on the PC connected to cycle ergometer Log in to the user account with the password: shsuser Open the Monark Anaerobic Test software Create a group if one isn t already created by clicking on add group, enter all the required fields Within this group, add a new participant by clicking on add person To run a wingate test, click run test If the protocol has already been made, select it in the protocol selection box, and then click continue. If the protocol has to be set up, select all the relevant options within the test settings box ensuring manually drop weight and automatically start recording is selected, and click create. Then select this protocol from the protocol selection box, and then click continue. Start the recording once the participant is warming up (NB. this will not start the 10 second sprint test). Once the test is over, save the file using participant ID code and visit number. Click on the relevant tab of analysis and export as an excel file Page 3 of 5

4 Conducting the 10 second Wingate test: Weigh the participant and calculate 7.5% body weight (0.075 x body weight), rounded to the nearest 100 g. Subtract 1 kg to account for the weight of the cycle ergometer weight cradle. Arrange disc weights to correspond with this weight. (E.g. for 70 kg reference participant: (0.075 x 70 kg) 1 kg = 4.25 kg.) Adjust the saddle height and position of the handle bars to suit the participant. There should be a slight bend in the leg when the pedal is at the bottom of its rotation. Handlebars are adjusted for participant comfort. Record saddle height and handle bar position. Attach 12-lead ECG and clinician to monitor throughout exercise and recovery for arrhythmias. Ask the participant to mount the bike, providing assistance if needed. Participants perform a 3 minute warm, cycling at a cadence of 60 rpm against a 1 kg resistance (i.e. with no weight on the weight cradle). Towards the end of the warm up, click start on the software. Prior to starting the test, remove all load from the bike by lifting up the weight cradle (by pulling cord on weight cradle, which is then held in place magnetically). Load the cradle with the pre-calculated load. Instruct the participant to start cycling against zero resistance at a steady cadence of 70 rpm (takes approximately 5 seconds). Once the participant is at 70 rpm, provide a 5 second countdown before applying the load by pressing the button on the right handle to drop the weight cradle (timer will start on PC Wingate software). Encourage the participant to cycle at maximal effort for the entire 10 seconds of the test and ensure participants remained seated throughout. At the end of the sprint, immediately remove the resistance by pulling on the weight cradle cord and allow the participant to actively recover (i.e. cool down ) against zero resistance for approximately 3 minutes. Page 4 of 5

5 Assist the participant off the bike once they feel ready to do so and provide water. If the participant feels faint or dizzy, immediately assist them off of the bike and lie supine with feet raised and provide water. Keep the participant in the lab for 20 minutes, making sure they feel ok before they leave. Computer software 9. DESIRED OUTCOME: Peak power output. Lowest power output. Mean power output. Fatigue index (percentage power decline between peak and lowest power output). 10. REFERENCES: Marsh, G. D., Paterson, D. H., Govindasamy, D., & Cunningham, D. a. (1999). Anaerobic power of the arms and legs of young and older men. Experimental Physiology, 84(3), Nieman, D. C., Gillitt, N. D., Shanely, R. A., Dew, D., Meaney, M. P., & Luo, B. (2013). Vitamin D2 Supplementation Amplifies Eccentric Exercise-Induced Muscle Damage in NASCAR Pit Crew Athletes. Nutrients, 6(1), Page 5 of 5

SPORT & MEDICAL PRODUCT RANGE

SPORT & MEDICAL PRODUCT RANGE MONARK LC7TT The ultimate research and performance analysis tool. The LC7TT is equipped with Shimano Di2 gear shifters which allow the cyclist to individually adjust the resistance