AapapeH yhueepcumem - I7noedue ArPAPHI4 HAYKI41 roduha III Spoil

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1 AapapeH yhueepcumem - I7noedue ArPAPHI4 HAYKI41 roduha III Spoil cblitocahmtapeh MOHIITOPIIHI HA 6011ECTIITE no J1ABAHMMATA PHYTOSANITARY MONITORING OF LAVENDER DISEASES Mai:Irma Hamm Mariana Nakova ArpapeH yhmeepcm-re-r - flnoartme Agricultural University - Plovdiv mnakova@yahoo.com Pemme B nepmoga r. OT CTballa t7 OCHOBM Ha pasknohehmwra, KaKTO t7 OT nmc-ra Ha nasahgynoem pac-rehin (Lavandula officinalis) CbC cmmn-romm Ha 3a6onmaHe, 6Fixa msonmpahm rb6m OT portase-re Phomopsis, Phoma, Septoria, Phytophthora, Alternaria, Fusarium m Botrytis. Ha ochoea-ra Ha mopconormyhm-re xaparrepmc-rmkm 6Fixa onpegenehm cnerthm-re amgoise: Phoma Iavandulae, Septoria Iavandulae, Phomopsis Iavandulae (Gabotto) Cif. &Vegni m Phytophthora nicotianae Breda de Haan, syn. Phytophthora parasitica (Dastur, 1913). Ph. Iavandulae e HOB na-roreh sa c-rpaha-ra, a Phytophthora parasitica ce yc-rahomea sa m.pem rrb-r no nasahrtyna-ra y Hac. Phomopsis Iavandulae arakyea cm6nata m Bow gl BHOCHBO oueemsahe Ha nmc-ra-ra. IlaTOreHbT o6pa3yea rimkhmgmm CbC sakpbmeha gl3 cna6o kpywoemgma copma m c gmamemp 87,5-105 um B ribpaecmha-ra. B TAX ce copmmpar rtaykne-rbyhm, enmncoemrthm no copma cnopm, neko npmcam-rm B cpega-ra m CbC saoc-rpehm KpaL.lu.ja, m C pasmepm 8,75-17,5x3,5-4,55 um. BpegoHocHoc-ri-a ce sacmnea npm acoummpahe Ha Phomopsis Iavandulae c Phoma Iavandulae m Septoria Iavandulae. Pac-reHmwra, sapasehm c rb6m OT port Phytophthora, mar 3a6aseH pac-rew, B OCHOBMTB Ha 6oni-m-re ne-ropacrim ce mist/tear cmeo-kacinem m BOBHMCTM nema, KOMTO BOBAT,B0 M3C1DXBaHe Ha cm6nata m xo.n-reehe m onagahe Ha nmc-ra-ra. Phytophthora ce M30.11Mpa OT pak hm o6pa3yeahmr B ochoea-ra Ha c-rb6no-ro. CnopaHrmm-re Ha rb6ata Bapmpar no copma OT lepmlihm Oo enmncoemrthm, c nanmna m C pasmepm 24,5x14,5 um. 001-OHMTB ca ccisepmyhm, C magkm CTeHM H C pasmepm 25,5x24,5 um. AH-repmgmm-re ca amcprmi-ihm, citepmyhm g0 FOLteemrtHm, c pasmepm 10-12x9-10 um. Oocnopm-re ca citepmyhm, anneopo-rmyhm, c pasmepm 24,5x24,5 um. Xnammgocnopm-re ca -repmmhan1-114 vinvl mh-repkanaphm, C pasmepm 13,6x13,6 um. OT kopehm-re Ha sarmhanm-re pac-rehmr ca msonmpahm m rb6m OT port Fusarium m Botrytis.143nm-raHm ca cpertc-rea sa koh-rpon Ha comoncmc no naaahrtynara no "in vitro" me-rortm. Abstract In the period , fungi of the genera Phomopsis, Phoma, Septoria, Phytophthora, Alternaria, Fusarium and Botrytis were isolated from diseased parts of lavender plants (Lavandula officinalis): stems, base of branches and leaves. Based on morphological characteristics, the following species were identified: Phoma Iavandulae, Septoria Iavandulae, Phomopsis Iavandulae (Gabotto) Cif. &Vegni and Phytophthora nicotianae Breda de Haan, syn. Phytophthora parasitica (Dastur, 1913). The last two are new pathogens for lavender in Bulgaria. Phomopsis Iavandulae attacks stems and gives grayish discoloration to the leaves. The pathogen forms roundish to pyriform picnidia, sized um, in the stem tissues. Conidia are two-celled, ellipsoid, slightly constricted/narrowed in the middle, with pointed ends, sized x um. The disease is more harmful in the cases of co-infection with Phoma Iavandulae and Septoria Iavandulae. Lavender plants infected with Phytophthora show suppressed growth. Grayish water-soaked spots are found at the stem base and cause stem wilt and death. Leaves turn yellow-grey in color and drop off. Phytophthora is isolated from the infected stem base. Fungi sporangia vary in shape from spherical to ellipsoid, with papillae, sized 24.5x24.5 um. Oogonia are spherical, with smooth walls, sized 25.5x24.5 um. Antheridia are amphigynous, spherical to oval, sized 10-12x9-10 um. Oospores are spherical, aplerotic, sized 24.5x24.5 um. Chlamidospores are terminal or intercalary, sized 13.6x13.6 um. Fungi from the genera Fusarium and Botrytis are also isolated from infected roots. Fungicides for the control of Phomopsis Iavandulae are tested "in vitro". Ith10,10BM Rpm nasahrtyna, rb6hm 6onec-rm, Phomopsis lavandula, Phytophthora nicotianae, syn. Phytophthora parasitica. Key words: Lavandula officinalis, fungal diseases, Phomopsis lavandula, Phytophthora nicotianae, syn. Phytophthora parasitica. 5

2 Agricultural University - Plovdiv ea AGRICULTURAL SCIENCES Volume III Issue flaBaHgyna-ra e e-repmyhomacneha KynTypa, KOATO ce otrnexcga rflabho 3a npom3b0gc-rbo Ha BMCOKO- Kayec-reeHo e-repmyho macno, koe-ro Hammpa npmnowehme B ko3me-rmka-ra, B napoomepm0hata m tiapmagelt-rmyhata npommumehoc-r, a cbuo TaKa - m B megmgmhata. CbL4BeTMATa ce non3bat B cbexco CbCTOAHme 3a e-repmyho macno, a B cyxo - B 6mnkoneyeHme-ro m apoma-repanmwra (F1HkynoB, 2000). 3a pogmha Ha nabahgyna-ra (Lavandula officinalis Ch.) ce cow Cpegm3emHomopckmwr permoh. B BbnrapmF1 e BHeceHa OT OpaHLImA npe r. OT K. ManKoB (KoewmKOB M gp., 1977). floybeho-krimma-rmyhm-re ycnobn y Hac 6naronpmwrc-rBaT He0HoTo pa3bm-rme. Pegmga permohm Ha c-rpahata - C-rapo3aropckm, BnaroeBrpagckm, WyMeHCKL.I, BapHeHckm m gp., ca ce M11M KaTO cnegmoyhm 3a KynTypaTa MMKpOpai"AOHM. WMpOKO pa3npoc-rpahehme armexcgahe-ro Ha nabahgyna nonyyaba B nepmoga , m oco6eho npe3b-ropa-ra nonobmha Ha MMHarIMA BeK, KOraTO nnou4m-re goctmrat OK gka ( r) (TonanoB m gp., 1989). 3a Bpeme-ro cneg 1990 r. nnouorre c nabahgyna ca yybc-rbm-renho orpahmyehm. 110BMWeH0 BHMmaHme Ha kyntypa-ra ce o-rgerm OTHOBO B HayanoTo Ha HaCTOAL1AMA XXI BeK. 1/13MCKBaHMATa Ha nabahgyna-ra KbM ycnobmwra Ha cpega-ra He ca BMCOKM. Bmpee Ha no-6egi-im m cna6o ankanhm noyibm, KaKBMTO B Bbnrapw M CTBaT (K0eWKMKOB t7 gp., 1977). 110HaCA HMCKM TemnepaTypm m 3acywaBaHe, HO He ce pa3bmbago6pe Ha BkmcneHm noyibm..flabahgyna-ra ce pa3mexcria Kam cpabhm-renho yc-rooymba KbM natorehhata mmkpocpopa. B nm-repatypara ce cpeu4a-r cbo6u4ehma 3a npomm OT cp-ronna3ma (wet feet), OT mogephobomo3amyhma Bmpyc (Alfalfa mosaic virus), 3a rb6hm 3a6onFiBaHmA - comoncmc (Phomopsis Iavandulae Gabotto), itoma (Phoma Iavandulae), cen-ropmo3a (Septoria Iavandulae Dezm.) m no4boo6m-rabauam natorehm OT pogobe-re Fusarium, Verticillium Phytophthora, KaTOCPTOCV0p0BOTO rhmehe e OCHOBHMAT npo6nem 3a goc-ra permohm Ha cbeta, rbge-ro ce otmexcga nabahgyna. B BbnrapmF1 mma cbo6u4ehma 3a Septoria Iavandulae, Phoma Iavandulae, kopehobo rhmehe m m3c-bxbahe Ha nabahgynata (nocnewm-re g Be C Hem3FicHeHa e-rmonormw MaprmHa, 2000). B yyxcgm nm-repatyphm M3TOLIHMLIM ce cbo6u4abat gahhm 3a u4e-rm, HaHeceHm OT Phomopsis Iavandulae (Hecbo6u4eHa y Hac). B enmcp-ro-rmyhm pa3mepm 6onecrra ce pa3bmba npe3 B-ropara nonobmha Ha XX BeK, Karam e yhmumoina m3l1fin0 nabahgynobm-re nnawragmm Ha Bb3pacT 3-4 rogmhm BbBI)paHLImA (Buczacki, Harris, 1998). flpe3 nocnewm-re rogmhm mi-itiopmagma 3a pa3npoc-rpahehme-ro m HaHeceHm-re 3ary6m no nabahgynobm HacaxcrieHmA OT tim-roclyropobm-re rb6m ce cpeu4a B ny6nmkagmm Ha Tsay (2002), Putnam (1991), Alvarez et al. (2007). B nepmoga r. B Ka-regpa "Om-rona-ronormA" B ArpapHIN yhmbepcm-ret - flnobgmb ce nonyyabaxa npo6m OT6onHm nabahgynobm pactehma C gen gmarhoc-rmka. Bneya-rneHmA 3a 3gpaBHOTO CbCTOAHMe Ha nabahgynobm-re HacaxcgeHMA ce Ha6paxa m npm m3b-bpuiehm o6cnegbahma B HAKOM pa0ohm Ha c-rpahata. Llama Ha HaCTOALLAOTO m3cnegbahe e Aa ce nonmpat m Aa ce onpegenwr cp-ronatorehm-re, npmgmhabauam 3a6onmaHmA m 3ary6m npm nabahgyna-ra 3a ycnobmwra Ha Bbnrapn B HaCTOALLAMA nepmog. MATEPIIAJ111 METOTill HanpaBeHa e makpockoncka gmarhoc-rmka m mmkpockonckm ahan113 Ha TbKaHM, B3eTM OT 6011HM pactehma, m Ha nona-rm, nonygehm Bbpxy KEIA. OnpegenFiHe-ro Ha cp-ronatorehm-re ce M3BbpLUM Ha ochoba Ha TexHm-re mopconormyhm m kyntypanhm oco6ehoc-rm m cmmn-romhm-re np0abm Ha 3a6onFiBaHmwra Bbpxy pactehmwra (Erwin&Ribeiro, 1996; Walker et al., 1998). 3a nonmpahe-ro Ha rb6m OT pog Phytophthora e m3non3baha H cenerrmbha cpega PARP [Corn meal agar amended after autoclaving (mg/ml) with pimaricin (10), ampicillin (250), rifampicin (10) and hymexazol (25)]. flatorehhocrra Ha nona-rm-re OT pogobete Phomopsis, Phoma m Septoria e goka3aha 4pe3 mhokynagma Ha mnagm pactehma (copt "XeMyc ", C BmcogmHa cm) CbC cnopoba cycneh3ma OT gHeBHa KynTypa Ha KnA. 3a m3ona-rm-re OT pog Phytophthora mskyctbehoto 3apa3FiBaHe ce m3b-bpuim 4pe3 c-repmnho HapaHRBaHe Ha pactehmwra B ochoba-ra CbC ckannen m noc-ramhe =ape Ha mmgenho 6nok4e OT gHeBHa KynTypa, nokpm-ro C BnaxceH TamnoH m napacpnm. PacTeHmwra ce noc-rauxa npm koh-rponmpahm ycnobin (TeMnepaTypa 26 C, Bb3gyuma Bnara 70%). HaamogeHmA 3a yc-rahombahe Ha >KM3HeHMA gmkbn Ha natorehm-re ce npobege Bbpxy 6onHm ne-ropacnm no nabahgynobm pactehl.1 m no TakmBa, oc-rabehm qa 3mmyBaT Ha noybehata 110BbpXHOCT. MMKpOCKOACKM ahanm3m ce m3b-bpumaxa npe3 mi-rrepban OT 2 cegmmgm, genorogmigho. C Llen KOHTpall Ha 3a6onFiBaHe-ro C npmt-imhm-ren Phomopsis Iavandulae no "in vitro" me-roga Ha TopH6bpm ce m3nm-ra ityhrmgmgho-ro ge0c-mme Ha npenapa-rm CbC cneghm-re aktmbhm 6a3m: nehkoha3on (Tona3 100 EK), gm(tekoha30n (ckop 250 EK), me-rmn Tmocl3aHaT (ToncmH M 70 Bfl), 6eHommn (6eHmmocT 50 Bfl), megeh xmgpokap6ohat (kocakg 101 H11); megeh okcmxnopmg m mahko3e6 (kynpogmh rong), gmagmahoh (genah 700 Bal. Onm-rm-re ce 3anaraxa B no -rpm nob-ropehma Ha BapmaHT m ce otym-raxa Ha 3--rmA m geh. PEWITATII B nepmoga r. 6Fixa ahanm3mpahm npo6m OT 6onHV1 nabahgynobm pactehn (Lavandula officinalis) 6

3 AapapeH yhueepcumem - I7noedue ArPAPHI4 HAYKI41 roduha III Spoil OT pakhwre Ha WyMeH, rlahareopmu.le Vl Ka3aHnbK. OT cm6nata Vl OCHOBMTe Ha pa3knohehmfrra Ha pactehmwra CbC umn-romm Ha 3a6onFiBaHe 6Fixa n0m/1pam/1 r1.614 OT pogobete Phomopsis, Phoma, Septoria, Phytophthora, Alternaria, Fusarium m Botrytis. Ha ochobata Ha KynTypanHfriTe oco6ehoctm Ha mmuenhwre KallOHMM, mopitonormghwre xaparrepwctim Vl pa3mepwre Ha nnoghwre Tena, KOHM,BMMTe vinvl cnopahriwre, KaKTO xnammgocnopwre, awrepmgmwre, oorohmtemoocnopwre 3a oommuetwre, 6Fixa onpegenehm BmgoBeTe Phomopsis Iavandulae, Phoma Iavandulae, Septoria Iavandulae m Phytophthora sp., KOMTO ce ogeptabat KaTo notehumanho BpegoHocHm natorehm 3a KynTypaTa (Ta6nmua 1). 3a6onFiBaHeTo c npmt-whwren Phomopsis Iavandulae (Gabotto) Cif.&Vegni (1964) e HOBO 3a abnrapin. rb6ata atakyba, Kam "y6mba cm6nata" Ha nabahgynatamte HexpoTiumpaTrIbpwre Bmgmmm npomm ce xaparrepiumpat C OFIOBHOCMBKaBO oubetabahe Ha nfrictata. Hai"A-Tfrinmt-Hfri ca cfrimntomwre, npm KOMTO otgenhfri mi-igmbmgm mrim rpyna pactehin BHe3anHo ymxbat, CTpeC, npegm3bmkah gaxcem6e33acywabahe. nmkobmte momehtm 3a 3a60nFiBaHeTo Ha0-4ecT0 ce ABABaT npe3 Tonnwre meceum - Eorm-aBrycT, Karam nfrictata Ha 3acerHame pactehin oxanbat (c r. 1). Ha6nEogaBa ce cna6 npmpact vinvl OTCbCTBMe Ha TaKbB. 11pM orneg Ha HacaxcrieHmwra ca xaparrephm cfrimn-romwre Ha ylbaxbahe Vl otmfripahe Ha urgenhfri pa3knohehiffi MI1M Ha uenfri pactehin. IlpVl aha111/13 Ha 60.11H wre TbKaHM B gbpbecm Ham Ha cm6nata ce OTKpMBaT nim-mgmwre Ha rb6ata. Te ca CbC 3axpbmeHa g0 cna6o xpywougha itopma n c gmamemp 87,5-105 um (c pr. 2). B TAX ce o6pa3ybat gbyxnerblihm t7 enfrincobmghm no itopma cnopm, nexo npmcbwrm B cpegatam CbC3aocTpeHm [Tanga, c pa3mepm 8,75-14,0-17,5x3,5-4,55 um (c pr. 3). Om/mai-114-re nnogi-m Tena t7 cnopm, TAxHaTa itopma pa3mepm CbOTBeTCTBaT Ha Bmga Phomopsis Iavandulae (Gabotto) Cif&Vegni, HOB natoreh 3a E.nrapiN. Taal-Riga Hm natorehm, n0m/1pam/1 OT 60ni-m nabahgynou pactehin npe3 nepmoga r. Table 1. Fungal pathogens, isolated from infected lavender plants from rla-rorehm - pog/bmg Pathogens - genus/specie rogmha Ha m30nmpahe Year of isolations aTM OT pac-rwrenhvi pram/ Isolated, from plant organs Phomopsis Iavandulae fle-ropacnii / shoots Phoma Iavandulae 2008 fle-ropacnii / shoots Septoria Iavandulae Miura / leaves Phytophthora sp OcHoBa Ha c-r-b6na-ra / stem base Alternaria sp Miura / KopeHm / leaves / roots Fusarium sp KopeHm / roots Botrytis sp KopeHm / roots HemgewrmcbinimpaHm Not identified KopeHm / roots cpua. 1. Cumnmomu Ha cpumodynopoeo ahuehe no naeahdynama Fig. 1. Phytophthora rot on lavender plants 7

4 Agricultural University - Plovdiv AGRICULTURAL SCIENCES Volume III Issue 'ua. 2. Cumnmomu Ha cpomoncuc no naeahdynama Fig. 2. Phomopsis symptoms on lavender plants Bpega-ra OT itomoncmca ce yeerimyaea npm acoummpahe C gpyrm nmki-imgmanhm natorehhm 1-1.6m OT pogoae-re Septoria m Phoma (Buczacki, Harris, 1998). Ilpm cen-ropmo3a-ra C npmymi-im-ren Septoria Iavandulae no rimc-ra-ra no pee Ha Bere-raumn-ra ce 110ABABaT yepeeho-kacinem netha, 3arpageHm C mh-reh3meh04epeeh BeHeu. 110-K1DCHO BueHTpanHara yac-r ne-rhata c-raeat CMIBM M no rophata VIM noinpxhour ce 06pa3yea-r nmki-imgmm nog copma-ra Ha yephm Toymum. Te ca Tonyec-rm, c pa3mepm um. Cnopwre ca HMLIJKOBMWM, npaem MI1M neko M3BMTM, C pa3mepm 16,5-30,5x1-1,5 um, egho- mrim gayknemyhm. npm como3a-ra C n pl4limh wren Phoma Iavandulae umn-romm-re ca nog copma-ra Ha TOLIKOBMWM yephm nema, KOMTO anocnegc-reme npmymhneat HanykaaHe (BKOpKO- BABaHe) Ha cm6na-ra m -re npmgo6meat kall3ne LIBAT. tlact OT cm6na-ra Ha naeafigynoem-re pactehmn 3armeaTabpxy 6011HW-1e TbKaHM ce OTKpMBaT rimkhmgmm Ha rb6ata. Te ca Tonyec-rm, yephm, C gmamemp um. Cnopm-re ca eghoknemyhm, 4,2-5,4x1,6-2,3 um. Ilpm mmkpockonckm Ha6m0geHmnB1D13Xy)KM3HeHMA umrbn Ha Tpm-re natoreha ce yc-rahoem, ye -re ce pa3bmbat ueriorogmumo KaTO nmkhmgmm C nmkhmgmocnopm no nopasehm-re oprahm Ha naeafigyna-ra. 3ana3eaT ce no pac-rm-renhm-re OCTaTIDLIM B noyea-ra mrim no Bere-rmpauAm pactehmn. Ycnoemn 3a no-macoao pasem-rme Ha 60nec-rm-re ce c1.3.gaeat npm yec-rm eanexcm m 06mriHm normakm. PacTeHmn-ra, HoceuAm 3apa3a OT Phytophthora sp., B pahhm-re ita3m mma-r 3a6aBeH pac-rew, Ha6mogaaa ce Kbn-reeHe Ha rimc-ra-ra, a anocnegc-reme yenxeahe m onagahe. Ha0-4ecT0 npes Eorim mrim aeryc-r rimc-ra-ra nanyyaeat CMBKaB KaTO CM.11H0 3acerHaTm-re ylbaxbat m m3c-bxea-r. Ha6nEogaea-rce m 6-bp30 yenxeahe, m3c-bxeahe t (113- Al? %":". cpua. 3. Ilimuduu u cnopu Ha Phomopsis lavandulae Fig. 3. Pycnidia and picnidiopsores of Phomopsis lavandulae 8

5 AapapeH yhueepcumem -17noedue AFPAPHI4 HAYKI4 roduha III Spoil Ta6nriqa MAHMe Ha HAKOM COyFirvii4mrivi Erbpxy pactexca Ha mmuena Ha Phomopsis Iavandulae Table 2. Effect of some fungicides on mycelia growth of Phomopsis Iavandulae OyHIMLIMP,M Fungicides KOHLIeFrrpaqvici, % Concentration, % Pac-rew Ha mmuena, e mm Mycelia growth, mm 3--rm,n,eH / 3ra day 6--rm,n,eH / 6m day ToncmH M / Topsin M CKop / Score genah / Delan Tona3 / Topas KocaKn, / Coside KynpoumH ron,n, / Kuprozin Gold EeHMMOCT / Benimost KoHTpona / Control m noxacoehreahe Ha crb6na-ra. OCHOBMTB Ha Taxmaa pac-rehmr mmat cmeo-xac[mem m BOBHMCTM netha, KOMTO ce pa3npoc-rpahrea-r BbB emcoymha m KbM KopeHa. flonyyehm-re nona-rm OT port Phytophthora BbpXy KEIA pa3bmbat 6Fm, nyme, c-reneu4 ce mmuen c pagmaneh pacte)k. CnopaHr'u.iTe eapmpat no itopma, HO npeo6nagaea-r-re3m c erimncoamwa itopma, c nanmna m c pa3mepm 24,5x14,5 um. OoroHm-re ca citepmyhm, anneopo-rmyhm, CbC cpewa BermymHa 25,5x24,5 um, c amcomrmi-mm ah-repm,rimm, citepmyhm QO ROLleamwm, m c pa3mepm 10-12x9-10 um. Oocnopm-re ca citepmyhm (24,5x24,5 um). Xnammgocnopm-re ca TepmmHanHm m mwrepxanaphm, OBallHM, C pa3mepm 13,6-13,6 um. Ha6mogaeaHm-re mopitonormyhm oco6ehoc-rm ca B CbOTIBBTCTBMB CbC cbo6u4ehm-re xaparrepmc-rmxm OT Hall (1993), Tsay (2002), Alvares et al. (2007), Putnam (1991), Dastur (1913) 3a Bv1ga Phytophthora nicotianae Breda de Haan, syn. Phytophthora parasitica, KoO-ro e HOB natoreh no naealtrtyna-ra B Em.nrapmFi. BpegoHocHoc-rra Ha rb6hm-re 6onec-rm ce3acmnea npm HacaxcrieHmR, cb3,0,agehm Ha 6e,0u-im noyem m c mere pea (ph=4-4,2). C-rpecoem cm-ryaumm, cabp3ahm CbC 3acywaBaHe m BMCOKM TemnepaTypm, B Kom6mHaumFi c natorehm, BO,BAT q0 macoao o6e3nmc-reahe Ha naealtrtynoem-re noceem. flpm mexahmyhm noepegm Ha KopeHoea-ra cmc-rema, ao,rieuam q0 pawbcaahe m nogcmyahe Ha xopehm-re, no HapaHeHm-re pac-rehmr npm 6naronpmFiTHm ycnoen ce pa3bmbat M natorehm OT pogoae-refusarium m Botrytis. nahhm-re OT "in vitro" onmtmte 3a BCOBKTMBHOCTTa Ha HAKOM nec-rmumgm coya-r, ye emcoxa ityhrmumwa aktmbhoct cpeuay Phomopsis Iavandulae nporefleat npenapa-rm-re ToncmH M 70 Bfl, cop 250 EK, Tona3 100 EK m KynpoumH rang, KOMTO mom 0a ce m3non3eat 3a 6op6a c natoreha (Ta6rimua 2). 5M01101-M4HMTB oco6ehoc-rm Ha com-ronatorehm-re gaea-rochoeahmega ce npenopbyat m npeaah-rmehm mem 3a KOHTpall, KOMTO mowega ce Kom6mHmpaTC HaapemeHHm Tpe-rmpaHMA C coyhrmumgm: Pe3m-r6a m m3rapfihe Ha 60.11HMTB oprahm; llonmekm c Bona, KOATO He npemmhaea npes c-rapm HacaKqeH MA; 1/13non3BaHe Ha 3,ripae nocagbyeh ma-repman; XmmmyHa 6op6a B pahhwre coehoc0a3m OT pasem-rme-ro Ha pactehmfrra m crieg KaTO ca otc-rpahehm 60.11Hwre ne-ropacrim. 113BOTIY1 Ha ochoea-ra Ha m3ebpwehm-re npoyyeahmr mowe qa ce Hanpaewr cnewm-re mmonm: 1. B naealtrtynoem-re HacaKqeHmR B HAKOM paoohm Ha BbnrapmR ce pa3emeat com-ronatorehhwre rb6m Phomopsis Iavandulae (Gabotto) Cif&Vegni m Phytophthora nicotianae Breda de Haan, syn. Phytophthora parasitica, HOBM natorehm no naealtrtyna-ra 3a E.nrapmFi. 2. Ha6nEogaaa ce acoummpahe Ha Phomopsis Iavandulae m Phytophthora nicotianae c natorehm-re OT porioae-re Phoma (P Iavandulae) m Septoria (S. Iavandulae) pm onm-rm "in vitro" ityhrmumgeh ecdekt abpxy mmuenhn pace Ha Phomopsis Iavandulae nporbrbat npenapa-rwre TOACMH M 70 Bfl, cop 250 EK, Tona3 100 EK m KynpoumH rong. 1111TEPATYPA AmaHacoea, M., Hedee H., E-repw4HomacneHm m nekapc-reehm KynTypm. Koed?KuKoe, Xp. u op., PacTeHmeawic-reo, CoitmR, 3emm3gaT. MapauHa, A., Bonec-rm no e-repw-homacnehm-re m neye6hwre pactehmr, CTapa 3aropa, copym. Tonanoe, B. u Op., PacTeHmem4c-reo, CoitmR, 3emm3gaT. RHKy.1708, M., OCHOBHM apoma-rmyhm pactehmr, CoitmR, ET "KIM -14B. MapKoea". 9

6 Agricultural University - Plovdiv ea AGRICULTURAL SCIENCES Volume III Issue Alvares, L. A., Perez-Sierra A., Armengol J., Garcia- Jimenes J., Characterization of Phytophthora nicotianae isolates causing collar and rot root of lavender and rosemary in Spain. -Journal of plant pathology, 89 (2): Buczacki, S. and Harris K., Phomopsis lavandulae causes shab disease, Lavander shab disease, Biolmages: The virtual field-guide (UK). Dastur, J. F., Phytophthora parasitica n.sp., a new disease on castor oil plant. - Mem. Dep. Agric. India, Bot. Ser., 5/4/: (cited in Tucker, 1933). Hall, G., An integrated approach to the analysis of variation in Phytophthora nicotianae and a redescription of the species. - Mycology research, 97: Orlikowski, L. B. and Voljuskaite A., New record of Phytophthora root and stem rot of Lavandula angustifolia. -ACTA Mycologica, 42 (2): Putnam, M., Root rot of lavender caused by Phytophthora nicotianae. - Plant pathology, 40 (3): Tsay, H. C., Root rot of lavender caused by Phytophthora parasitica in Taiwan. - Plant pathology bulletin, 11: Walker, J. M., Rhichie B. J., Holderness M., Plant clinic handbook, CAB International, UK. lavenderbrrhtm Technical notes: Lavender- introduction. CmamuRma e npuema Ha a. PegeH3eHm - ripoch. dch Cmoilgo Kapoe profkarov@yahoo.co.uk 10