PRDM DPPA IFITM TERT

Transcription

1 ES ips ES ips oogonial stem cells ; OSCs Cryotissue RNA helicase DDXDEAD box polypeptide FACS OSCs OSCs m PRDMDPPAIFITMTERT mrna OSCs MEF feeder MEF mrna OSCs m DDXKIT,YBXLHX mrna OSCs DMC SYCPFACS DNA n OSCs GFP m GFP GFP OSCs GFP OSCs GFP GFP LHX YBX YBX OSCs GFP

2 DDX4 FACS 1-3 GFP GFP GFP 7-14 GFP OSCs GFP GFP OSCs OSCs OSCs OSCs Tilly DDX Ddx FACS Tilly IfitmFragilis helicase Ddx OSCs Stra OSCs ESCs PGCs OSCs ESCs PGCs Liu Cre-loxP Ddx Ddx4-Cre ; Rosa26 rbw/+ m Ddx Tilly Ddx4-Cre ; Rosa26 tdtm/+ tdtm Ddx FACS tdtm OSCs Liu Tilly DDX FACS DDX DDX EGFP EGFP Tilly Liu OSCs

3 ips in vitro maturaon / Tilly HSVtk Stra GCV HSVtk GCV Stra Tilly Wu OSCs Silvestris OSCs m GDF-9 SYCP3 mrna DPPA3 mrna OSCs OSCs MII in vitro MII OSCs in vitro maturationivm MII OSCs in vitro OSCs OSCs IVF ART DNA OSCs ICSI ART ART OSCs

4 OSCs OSCs ICSI ICSI ICSI OSCs ICSI OSCs OSCs OSCs ART DNA OSCs ART Johnson J, Canning J, Kaneko T, Pru JK, Tilly JL Germline stem cells and follicular renewal in the postnatal mammalian ovary. Nature, -. Zou K, Yuan Z, Yang Z, Luo H, Sun K, Zhou L, Xiang J, ShiL,YuQ,ZhangY,HouR,WuJProduction of offspring from a germline stem cell line derived from neonatalovaries.natcellbiol, -. White YA, Woods DC, Takai Y, Ishihara O, Seki H, Tilly JLOocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women. Nat Med, -. Kagawa N, Silber S, Kuwayama MSuccessful vitrification of bovine and human ovarian tissue. Reprod Biomed Online, -. Woods DC, White YA, Tilly JLPurification of oogonial stem cells from adult mouse and human ovaries : an assessment of the literature and a view toward the future. Reprod Sci, -. Woods DC, Tilly JLIsolation, characterization and propagation of mitotically active germ cells from adult mouse and human ovaries. Nat Protoc, -. Imudia AN, Wang N, Tanaka Y, White YA, Woods DC, Tilly JLComparative gene expression profiling of adult mouse ovary-derived oogonial stem cells supports a distinct cellular identity. Fertil Steril in press. Imudia AN, Wang N, Tanaka Y, White YA, Woods DC, Tilly JLComparative gene expression profiling of adult mouse ovary-derived oogonial stem cells supports a distinct cellular identity. Fertil Steril, -. Zhang H, Zheng W, Shen Y, Adhikari D, Ueno H, Liu K Experimental evidence showing that no mitotically active female germline progenitors exist in postnatal mouse ovaries. Proc Natl Acad Sci USA, -. Park ES, Tilly JLUse of DEAD-box polypeptide- Ddxgene promoter-driven fluorescent reporter mice to identify mitotically active germ cells in post-natal mouse ovaries. Mol Hum Reprod, -. Zhang H, Panula S, Petropoulos S, Edsgard D, Busayavalasa K, Liu L, Li X, Risal S, Shen Y, Shao J, Liu M, Li S, Zhang D, Zhang X, Gerner RR, Sheikhi M, Damdimopoulou P, Sandberg R, Douagi I, Gustafsson JA, Liu L, Lanner F, Hovatta O, Liu KAdult human and mouse ovaries lack DDX-expressing functional oogonial stem cells. Nat Med, -. Woods DC, Tilly JLReply to Adult human and mouse ovaries lack DDX-expressing functional oogonial stem cells. Nat Med, -. Wang N, Satirapod C, Ohguchi Y, Park ES, Woods DC, Tilly JLGenetic studies in mice directly link oocytes produced during adulthood to ovarian function and natural fertility. Sci Rep,. Ding X, Liu G, Xu B, Wu C, Hui N, Ni X, Wang J, Du M, Teng X, Wu JHuman GV oocytes generated by mitotically active germ cells obtained from follicular aspirates. Sci Rep,.

5 Silvestris E, Cafforio P, D Oronzo S, Felici C, Silvestris F, Loverro G In vitro differentiation of human oocytelike cells from oogonial stem cells : single-cell isolation and molecular characterization. Hum Reprod, - Telfer EE, McLaughlin M In vitro development of ovarian follicles. Semin Reprod Med, -. McLaughlin M, Albertini DF, Wallace WHB, Anderson RA, Telfer EEMetaphase II oocytes from human unilaminar follicles grown in a multi-step culture system. Mol Hum Reprod, -. Fakih MH, Shmoury MEl, Szeptycki J, dela Cruz DB, Lux C, Verjee S, Burgess CM, Cohn GM, Casper RF The AUGMENTSM Treatment : Physician Reported Outcomes of the Initial Global Patient Experience. JFIV Reprod Med Genet,. Takai YRecent advances in oncofertility care worldwide and in Japan. Reprod Med Biol doi :. /rmb.. Telfer EE, Albertini DFThe quest for human ovarian stem cells. Nat Med, -. Woods DC, Tilly JLThe nextregeneration of ovarian biology and fertility in women : is current science tomorrow s practice? Fertil Steril, -.