TRAINING & EXPERTISE REPRODUCTION BIOTECHNOLOGIES

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1 TRAINING & EXPERTISE

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3 THE VALUE OF TRAINING 4 EQUINE SEMEN ANALYSIS AND PROCESSING 6 ADVANCED EQUINE REPRODUCTION WORKSHOP 8 BOVINE SEMEN PROCESSING & REPRODUCTION 10 BOVINE IN VITRO EMBRYO PRODUCTION 12 ADVANCED SWINE REPRODUCTION WORKSHOP 14 ON-SITE SWINE FREEZING WORKSHOP 16 ON-SITE OVINE ENDOSCOPY INSEMINATION WORKSHOP 18 RABBIT SEMEN PROCESSING 20 AQUATIC SPECIES BREEDING PROGRAMS 22 AQUATIC SPECIES SEMEN CRYOPRESERVATION 24 POULTRY SEMEN PROCESSING 26 3

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5 THE VALUE OF TRAINING... Because your human capital is paramount, we understand the value of building skills and providing you with the know-how and expertise to create more value for your organization. Whether at our head office or on-site, IMV Technologies provides its worldwide base of clients with training and audits catered to their own industries (cattle, pig, poultry, horse, rabbit, goat, fish, dog, cat and camel). IMV Technologies can also help you measure and monitor progresses and come up with solutions to real life problems. Contact us to see how our global training team can enable your people further. 5

6 EQUINE SEMEN ANALYSIS & PROCESSING PRESENTATION AIM OF THE COURSE Obtain theoretical and practical knowledge on equine semen processing and analysis. INTENDED AUDIENCE DVM s, Scientists, breeding technicians. WORKSHOP DURATION 1 day. WHERE IMV-Technologies R&D Saint Ouen sur Iton France. REGISTRATION DEADLINE 6 weeks before the workshop. TEACHING TOOLS During the course, the participants will receive alternatively theoretical and practical guidelines. Participants will achieve practical steps in semen processing and analysis. PARTICIPANTS Max. 4 people. 6

7 PROGRAM Morning The advantages of artificial breeding Semen collection procedures Semen evaluation by photometry Microscopic evaluation Afternoon Use of CASA to evaluate semen motility parameters Introduction to flow cytometry Use of flow cytometer to analyse semen traits Conclusions, discussions 7

8 ADVANCED EQUINE REPRODUCTION WORKSHOP PRESENTATION AIM OF THE WORKSHOP Obtain theoretical and practical knowledge on equine semen processing and analysis. INTENDED AUDIENCE DVM s, Scientists, breeding technicians. WORKSHOP DURATION 2 days. WHERE Ref Ref National Stud Haras du Pin (IFCE), IMV-Technologies R&D Saint Ouen sur Iton France. At customer location, after purchase of equipment. REGISTRATION DEADLINE 6 weeks before the workshop. TEACHING TOOLS During the course, the participants will receive alternatively theoretical and practical guidelines. Participants will achieve practical steps in semen processing and analysis. PARTICIPANTS Ref Max. 4 people. Ref Max. 8 people. 8

9 PROGRAM DAY 1 Morning The advantages of artificial breeding Stallion management AV preparation Semen collection procedures Afternoon Semen evaluation by photometry Microscopic evaluation Use of CASA to evaluate semen motility parameters Introduction to flow cytometry (if applicable) Use of flow cytometer to analyse semen traits Semen packaging, identification, freezing Post-thaw evaluation DAY 2 Morning Mare management Ovaries ultrasonic detection Insemination with fresh/chilled/frozen-thawed semen Embryo transfer Afternoon Embryo transfer technology 9

10 BOVINE SEMEN PROCESSING & REPRODUCTION PRESENTATION AIM OF THE WORKSHOP Obtain theoretical and practical knowledge on bovine semen analysis and processing. INTENDED AUDIENCE DVM s, Scientists, breeding technicians. WORKSHOP DURATION 2 days. WHERE Ref Ref At customer location, after purchase of equipment. IMV R&D laboratory Saint Ouen sur Iton France. REGISTRATION DEADLINE 6 weeks before the workshop. TEACHING TOOLS During the course, the participants will receive alternatively theoretical and practical guidelines. Participants will achieve practical steps in semen processing and analysis. PARTICIPANTS Ref Max. 8 people. Ref Max. 4 people. 10

11 PROGRAM DAY 1 Morning The advantages of artificial breeding Bull management AV preparation Semen collection procedures Afternoon Semen evaluation by photometry Microscopic evaluation Use of CASA to evaluate semen motility parameters Introduction to flow cytometry (if applicable) Use of flow cytometer to analyse semen traits (if applicable) Packaging, identification, freezing Post-thaw evaluation DAY 2 Morning Oestrus management Insemination frozen-thawed semen Semen collection, evaluation, dilution Afternoon Packaging, identification, freezing Post-thaw evaluation Conclusions/discussions 11

12 BOVINE IN VITRO EMBRYO PRODUCTION PRESENTATION AIM OF THE WORKSHOP Obtain theoretical and practical tools needed during the in vitro bovine embryo production. INTENDED AUDIENCE Veterinarians, biologists, scientists, lab technicians. WORKSHOP DURATION 2-3 days. WHERE Embryology Lab, GETI team, University of Grenoble, Grenoble France. REGISTRATION DEADLINE 4 weeks before the workshop. TEACHING TOOLS During the training, theoretical and practical modules will be alternated during the different parts of the process, including, oocyte grading, handling, and the three principal steps of the in vitro embryo production: oocyte maturation, fertilization and embryo culture. The retrieval of cumulus oocytes complexes (COC s) from slaughterhouse ovaries is envisaged. PARTICIPANTS Max. 4 people. 12

13 PROGRAM THEORY In vitro embryo production: basic remarks Media preparation for the different steps of the process Oocyte retrieval. Oocyte maturation control, staining and microscopic techniques Embryo quality evaluation, differential staining and apoptosis indicator PRACTICE Oocyte retrieval from slaughterhouse ovaries COC s grading and handling In vitro maturation Semen thaw, preparation and quality evaluation. before in vitro fertilization Matured oocyte handling In vitro Fertilization Zygote evaluation and preparation for culture Culture of presumptive zygotes 13

14 ADVANCED SWINE REPRODUCTION WORKSHOP PRESENTATION AIM OF THE WORKSHOP Obtain theoretical and practical knowledge on swine semen freezing. INTENDED AUDIENCE DVM s, Scientists, breeding technicians. WORKSHOP DURATION 1 day. WHERE Ref Ref IMV-Technologies s lab Saint Ouen sur Iton France La Coliniere boar stud, Laval France. At customer location, after purchase of equipment. REGISTRATION DEADLINE 6 weeks before the workshop. TEACHING TOOLS During the course, the participants will receive alternatively theoretical and practical guidelines. Participants will achieve practical steps in semen processing and analysis. PARTICIPANTS Ref Max. 3 people. Ref Max. 6 people. 14

15 PROGRAM Morning The advantages of artificial breeding Semen collection procedures Semen evaluation by photometry Microscopic evaluation Afternoon Use of CASA to evaluate semen motility parameters Use of flow cytometry for semen analysis Cooling, packing Insemination tools and procedure Conclusions, discussions 15

16 ON-SITE SWINE FREEZING WORKSHOP PRESENTATION AIM OF THE WORKSHOP Obtain theoretical and practical knowledge on swine semen freezing. INTENDED AUDIENCE DVM s, Scientists, breeding technicians. WORKSHOP DURATION 2 days. WHERE At customer location, after purchase of equipment. REGISTRATION DEADLINE 6 weeks before the workshop. TEACHING TOOLS During the course, the participants will receive alternatively theoretical and practical guidelines. Participants will achieve practical steps in semen processing and analysis. PARTICIPANTS Max. 6 people. 16

17 PROGRAM DAY 1 Morning The advantages semen freezing Boar management Semen evaluation by photometry Microscopic evaluation Use of CASA to evaluate semen motility parameters Afternoon Semen media Centrifugation Packaging, identification, freezing Post-thaw evaluation DAY 2 Morning Oestrus management Introduction to AI tools Insemination with frozen/thawed semen Semen collection, evaluation, dilution Afternoon Packaging, identification, freezing Post-thaw evaluation Conclusions/discussions 17

18 ON-SITE OVINE ENDOSCOPY INSEMINATION WORKSHOP PRESENTATION AIM OF THE COURSE Obtain theoretical and practical knowledge on ovine frozen semen insemination. INTENDED AUDIENCE DVM s, Scientists, breeding technicians. WORKSHOP DURATION 3 days. WHERE At customer location, after purchase of equipment. REGISTRATION DEADLINE 6 weeks before the worshop. TEACHING TOOLS During the course, the participants will receive alternatively theoretical and practical guidelines. Participants will achieve practical steps in insemination by endoscopy. PARTICIPANTS Max. 6 people. 18

19 PROGRAM DAY 1/2/3 Frozen semen: how to manipulate straws Thawing Use of endoscope-trocar Ewe preparation Safety: mistakes to avoid Research of semen insemination site Semen deposition Removal of tools and care Ewe: post endoscopy care Cleaning disinfection Summary discussion Upon customer request, day 1 can be used to: - collect semen - dilute, cool, freeze semen - analyse frozen/thawed semen 19

20 RABBIT SEMEN PROCESSING PRESENTATION AIM OF THE WORKSHOP Obtain theoretical and practical knowledge on rabbit semen analysis and processing. INTENDED AUDIENCE DVM s, Scientists, breeding technicians. WORKSHOP DURATION 1 day. WHERE Ref Ref At customer location, after purchase of equipment. IMV-Technologies R&D Saint Ouen sur Iton France. REGISTRATION DEADLINE 6 weeks before the workshop. TEACHING TOOLS During the course, the participants will receive alternatively theoretical and practical guidelines. Participants will achieve practical steps in semen processing and analysis. PARTICIPANTS Ref Max. 10 people. Ref Max. 4 people. 20

21 PROGRAM Morning The advantages of artificial breeding Semen collection procedures Microscopic evaluation Semen dilution Semen packaging Afternoon Use of CASA to evaluate semen motility parameters Insemination of rabbit does Conclusions, discussions 21

22 AQUATIC SPECIES BREEDING PROGRAMS PRESENTATION AIM OF THE COURSE Theoretical and practical knowledge about breeding program, inbreeding management, mating plan design, breeding values, selection index. INTENDED AUDIENCE DVM s, scientists, hatchery operator. WORKSHOP DURATION From 1.5 to 3 days. SPECIES Freshwater, marine species, fish, molluscs, mammals (salmonids, bass, bream, turbot, grouper, tilapia, sturgeon, barramundi, pangasius, oyster, mussel, cucumber). WHERE On-site, hatchery, lab. REGISTRATION DEADLINE 6 weeks before the workshop. TEACHING TOOLS During the course, the participants will receive alternatively theoretical and practical guidelines. PARTICIPANTS Max. 15 people. 22

23 PROGRAM THEORY Breeding programs case studies Genetic improvement (index creation, financial impact, inbreeding control) Quantitative genetics (gene definition, trait definition, genotype/phenotype, trait transmission, phenotypic value, heritability, breeding value) How to conduct a breeding program? (mating, selection pressure, computation, selection pressure calculation, individual tagging) Genomics (Parentage assignment, QTL, Genome uses) The advantages of artificial breeding and cryopreservation PRACTICAL Complex mating plans Management and creation of a genetic database Big data recording biometry, field trials 23

24 AQUATIC SPECIES SEMEN CRYOPRESERVATION PRESENTATION AIM OF THE COURSE Theoretical and practical knowledge about semen analysis, processing and cryopreservation. INTENDED AUDIENCE DVM s, scientists, hatchery operators. SPECIES Freshwater, marine species, fish, molluscs, mammals (salmonids, bass, bream, turbot, grouper, tilapia, sturgeon, barramundi, pangasius, oyster, mussel, cucumber). WORKSHOP DURATION From 1.5 to 3.5 days. WHERE Ref Ref On-site, hatchery, lab. IMV lab, L Aigle France. REGISTRATION DEADLINE 6 weeks before the workshop. TEACHING TOOLS During the course, the participants will receive alternatively theoretical and practical guidelines. Participants will achieve practical steps in semen analysis and processing. PARTICIPANTS Max. 15 people. 24

25 PROGRAM THEORY The advantages of artificial breeding and cryopreservation Cell freezing theory Semen collection and conservation Semen evaluation by photometry Semen evaluation by microscopy External speaker 1 (video conference): Semen quality criteria External speaker 2 (video conference): Main parameters for salmonid freezing Dimension of a cryopreservation lab Use of freezing in production New practices in reproduction PRACTICAL Semen collection and conservation Semen evaluation by photometry Semen evaluation by microscopy Freezing preparation Semen dilution Packaging, freezing Post-thaw evaluation Fertilization 25

26 POULTRY SEMEN PROCESSING PRESENTATION AIM OF THE COURSE Obtain theoretical and practical knowledge on poultry semen analysis and processing. INTENDED AUDIENCE DVM s, Scientists, breeding technicians. WORKSHOP DURATION 1 day. WHERE Ref At customer location, after purchase of equipment. Ref IMV-Technologies laboratories. Saint Ouen sur Iton France. REGISTRATION DEAD LINE 6 weeks before the workshop. TEACHING TOOLS During the course, the participants will receive alternatively theoretical and practical guidelines. Participants will achieve practical steps in semen processing and analysis. PARTICIPANTS Ref Max. 10 people. Ref Max. 3 people. 26

27 PROGRAM Morning The advantages of artificial breeding Semen collection procedures Microscopic evaluation Semen dilution Semen packaging Afternoon Insemination of female Conclusions, discussions 27

28 IMV Technologies France ZI n 1 Est L Aigle +33 (0) contact@imv-technologies.com IMV Technologies U.S.A th Avenue North Maple Grove, MN contact@imvusa.com IMV Technologies Italy Via Stefano Merli Piacenza info@imv-technologies.it IMV Technologies India Plot No 750, Phase V, Udyog Vihar Gurgaon (Haryana) /31/32 contact@imvindia.com IMV Technologies China 12F, Unit A. No. 400, Pudian Road, Pudong Shanghaï - China contact@imvchina.com IMV Technologies Netherlands Amperestraat AD Leeuwarden info@nifa.nl Cryo Bio System France BP 42 - Rue Jean Moulin F78512 Rambouillet Cedex +33 (0) contact@cryobiosystem-imv.com Polysem France BP 42 - Rue Jean Moulin F78512 Rambouillet Cedex +33 (0) contact@imv-technologies.com V06.16