Computer Assisted Semen Analysis. M i r a S p e r m A n a l y z e r

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1 Computer Assisted Semen Analysis M i r a 9 S p e r m A n a l y z e r

2 CASA Mira9 Sperm Analyzer Mira9 (CASA) Specifications System Components 1. PC include Mira9 CASA software. 2. Mira9 special slide. 3. Tempreature controller. 4. Soft key. Database The in-built database enables the user to store, backup and restores any kind of obtained information. The in-built database enables the user to generate a historical report for any patient. The in-built database is capable of helping researcher by generating many statistics reports. Other features of Mira 9 Software Mira 9 software is able to automatically add appropriate comments according to the patient results. Mira 9 software has the ability to generate a custom reports like urine, stool, CBC and bone marrow with any captured image using the system digital camera and microscope. Mira 9 software allows the user to record and print the manual test result of semen. System Requirments 1. Trinoculer Microscope with plan objective lens. 2. CCD camera ( Minimum48TV line). 3. Color printer. Standard Methods Mira 9 CASA includes two standard methods, Dynamic and Morphology, a facility for automatic report generation, and an in-built database. The analysis of the parameters is performed according to the recommendations given by the Worldwide Health Organization (WHO) for the 4th edition (latest 5th edition is available as well). The parameters that can be determined with help of the Mira 9 CASA are the matin ones for estimation of the sperm fertility. Dynamic Standard Method The method is designed to estimate the spermatozoa concentration and their motility in a native specimen. Determining spermatozoa concentration in ( Million/ ML) Measuring spermatozoa movement parameters and classifying spermatozoa into motility classes in accordance with WHO recommendations. Measuring spermatozoa dynamic parameters ( VCL, VSL, VAP, MAD, ALH, BCF, LIN, WOB and STR) Morphology Standard Method The method is designed to determine the morphological parameters of spermatozoa. The software of 5th edition morphology can automatically calculate TZI, SDI and MAI parameters.

3 MiraLab Tel / sales@mira-lab.com The system follows WHO (21) strict criteria for motility patterns & morphometric assessment of human semen. Case no Name Date 212/2/21 Lab ID 33 Ref. by Age 3 Years Collection Masturbation at lab Abestination 4 Days Physical properties Volume ph Color Odor Viscosity Liquefaction time Liquefaction state Test result Test Concentration (million/ml) Total sperm no. (million) Progressive motility (PR) Total Motility (PR+NP) Morphology index Vitality Agglutination & Aggregation Agglutination Nil Agglutination grade - Cells other than sperms Grey Opalescent Normal Normal 45 min. Complete > 1.5 ml > 7.2 > 58 _ _ 2 _ Result Status Reference Value White Blood Cells - 1 < 5 /H.P.F Red Blood Cells - 1 <5 /H.P.F Spermatogenic cells 3-5 /H.P.F Epithelial cells - 1 /H.P.F > 32 > 4 > 15 (million/ml) Non-specific Aggregation Nil 61. PR > 39 (million/ejaculate) > 4 normal is accepted Pinheads NP 25.2 Immotile million/ml Pinheads sperms are not counted as spermatozoa or head defects because they possess no chromatin or head structure anterior to the basal plate. Computer Assisted Semen Analysis - Mira-9 CASA - Welli ISO91 - ISO13485 Comment Normal seminal profile The semen passed the W.H.O. strict criteria for count, motility and morphology.

4 MiraLab Tel / sales@mira-lab.com Mr. Tamer Ragab Ibrahim Case no Dynamic Analysis Report (CASA - WHO 21) Dynamic Parameters Report ( I ) Classification Conc. (million/ml) Total number (million) Percentage Tested sperm Total motility (PR+NP) Progressive motility (PR) Non progressive (NP) Immotile (IM) * Progressive motility (PR) spermatozoa moving actively, either linearly or in a large circle, regardless of speed. * Non-progressive motility (NP) all other patterns of motility with an absence of progression, i.e. swimming in small circles, the flagellar force hardly displacing the head, or when only a flagellar beat can be observed. * Immotile (IM) no movement. Dynamic Parameters Report ( II ) VCL [µm/s] MAD['] LIN 62.5 VSL [µm/s] 29.7 ALH [µm] 4.54 WOB VAP [µm/s] BCF [Hz] 4.7 STR VCL Curvilinear velocity MAD Mean Angular Degree LIN Linearity (VSL/VCL) VSL Straight line Velocity ALH Amplitude of Lateral head Disp. WOB Wobble (VAP/VCL) VAP Average Path Velocity BCF Beat-cross Frequency STR Straightness (VSL/VAP) Sperm Tracks & Velocities Computer Assisted Semen Analysis - Mira-9 CASA - Welli - ISO91 - ISO13485 Name

5 MiraLab Tel / sales@mira-lab.com Name Mr. Tamer Ragab Ibrahim Case no Semen Analysis Charts (CASA - WHO 21) Progressive Velocity (VSL) Track Speed (VCL) 1 _ 1 _ 8 _ 8 _ 6 _ 6 _ 4 _ _ _ >6 µm/s Path Velocity (VAP) µm/s >6 Sperm Distribution 1 _ 1 _ 8 _ 8 _ 6 _ 6 _ 2 _ _ _ _ µm/s 13.8 >6 Sperm Track Image PR NP Immotile Computer Assisted Semen Analysis - Mira-9 CASA - Welli - ISO91 - ISO _

6 MiraLab Tel / sales@mira-lab.com Name Mr. Tamer Ragab Ibrahim Case no Normal Sperms (Morphology Index) 2. Terato Sperms 8. A. Head Abnormality _ > 4 is accepted ( ) C. Principal piece (Tail) Abnormality Big Head Short Tail 5 Small Head 5 Bent Tail 5 Tapered Head 15 Multi-Tail Pyriform Head 1 Tail Break Down Coiled Tail Irregular Tail Round Head Amorphous 4 Vacuolated Small Acrosome Double Head Thin Neck Sharply Bent Class Multiple Anomalies Index (MAI) The mean number of anomalies per abnormal spermatozoon. TZI 1.23 TeratoZoospermic Index (TZI) Total number of defects divided by the number of abnormal sperms. 1 Asymmetrical insertion E.R.C. MAI B. Neck & Midpiece Abnormality Thick or Irregular D. Excess Residual Cytoplasm (E.R.C.) SDI.98 Sperm Deformity Index (SDI) Total number of defects divided by the number of sperms counted. 5 Head Length (um) Head Width (um) Length/Width () Head Area (um ) Head Perimeter (um) Acrosome () Mean of Normal Sperm Mean of Terato Sperm Reference values Computer Assisted Semen Analysis - Mira-9 CASA - Welli - ISO91 - ISO13485 Morphology Analysis Report (CASA - WHO 21)

7 MiraLab Tel / sales@mira-lab.com Mr. Tamer Ragab Ibrahim Case no Computer Assisted Semen Analysis - Mira-9 CASA - ISO91 - ISO13485 Name Normal sperm Abnormal sperm

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