FEATURES. 26 Coda the unsung hero

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4 CONTENTS Fertility Magazine The First Magazine In Fertility TM FEATURED ON THE COVER The Ultrastructure of a Human Giant MII Oocyte: A Case Report by Ana S. Lopesa, Elsa Oliveira, Mariana Cunha, Joaquina Silva, Alberto Barros, Mário Sousa Patient s Corner Comparative Growth Behaviour and Characterization of Stem Cells from Human Wharton s Jelly by CY Fong, M Richards, N Manasi, A Biswas, A Bongso S 3 Vitrification System: a Novel Approach to Blastocyst Freezing by James J. Stachecki, PhD and Jacques Cohen, PhD Successful vitrification in a closed carrier device of blastocysts originating from infertile patients, egg donors, or in-vitro maturation by J Lopez, NH Zech, P Frias, P Vanderzwalmen Patient s Corner by Michele Brown, MD, FACOG FEATURES 26 Coda the unsung hero 37 Using an IVF Database to Influence Real Outcomes by John Moschella, MS ELD 44 A single medium and sequential media are equally effective for the culture of human embryos to the blastocyst stage: a pilot study by Danielle T Schneider, Sidney Verza Jr. & Sandro C Esteves 48 Somatic Cell Nuclear Transfer in Its First and Second Decades: Successes, Setbacks, Paradoxes and Perspectives by Dr. Gábor Vajti 4 FERTILITY MAGAZINE VOLUME 11

5 59 A Prospective Randomized Comparison of global Medium with Sequential Media for Culture of Human Embryos to the Blastocyst Stage by Don Rieger, PhD 62 Ovarian Tissue Cryobanking: Perspectives for Assisted Reproduction by Vladimir Isachenko 68 Guidelines for Handling Infectious Patients in the IVF Laboratory by Sangita Jindal, PhD, HCLD 70 The Contribution of Rescue Spontaneous Maturation in Controlled Ovarian Stimulation Cycles of Poor Responder Patients by Daniela Paes de Almeida Ferreira Braga, DVM, MSc, Rita de Cássia Sávio Figueira, MSc, Camila Madaschi. BSc, Renata Cristina Ferreira, MSc, Assumpto Iaconelli Jr., MD, Edson Borges Jr. MD, PhD 62 Ovarian Tissue Cryobanking: Perspectives for Assisted Reproduction 74 Is the Laparoscopy and Dye Test More Useful in Low-risk Infertile Women? by Jitendra G Jadhav MRCOG and Mohamed Hefni FRCOG 88 New Products 90 Books 92 Resources 94 Conferences Instructions to Contributors To submit an Article/Abstract us at: editor@ivfonline.com Note: Articles and Abstracts must be accompanied by a photo of the author(s). To submit an Ad us at: advertise@ivfonline.com Editor in Chief: Monica Mezezi, MBA Editor(s): Don Rieger, PhD Assistant Editor: Michael West Design: Debrah Frank Editorial Office: IVFonline, 24 Norwich St. E. Guelph, Ontario, Canada, N1H 2G6 US/Canada: International: Fax: editor@ivfonline.com Fertility Magazine and all its associates 2009, All Rights Reserved. Covers, contents, images, ads in print or web form are copryight protected and reprinting or reproduction of any kind is expressly prohibited without the written permission of Fertility Magazine. Fertility Magazine does not knowingly accept false or misleading advertising, articles, opinions or editorial, nor does the publisher assume any responsibility for the consequences that occur should any such material appear, and assumes no responsibility for content, text, opinions or artwork of advertisements appearing in Fertility Magazine in print or web form. Some of the views expressed by contributors may not be the representative views of the publisher. VOLUME 11 FERTILITY MAGAZINE 5

6 zenith biotech Stem Cells & Reagents for Research & Drug Discovery StemQ Zenith Biotech announces the launch of a new line of stem cell qualified products. Our StemQ product line includes stem cells (from embryonic and adult sources), media, matrix and reagents tested and qualified for a wide variety of cell types and applications. Our proprietary media formulations are designed to support specific lineage stages such as growth of the stem cells, differentiation into a specific lineage stage and maintenance of stem cells, progenitors and differentiated cell types. StemQ products are rigorously tested and qualified for each application in an ISO certified facilty. Investigators can order with confidence knowing the products were qualified for their application and do not require additional extensive screening of lots in their laboratories. Complete instructions accompany each product as well as a thorough description of the test results for each lot. Buy StemQ products with confidence. Our StemQ products are designed and qualified to simplify using stem cell technology. Media DMEM 4.5gm/L Glucose, with NaPyruvate, w/o L-Glutamine, Osmolarity optimized for ES Cells DMEM/F12 4.5gm/L Glucose, with NaPyruvate, w/o L-glutamine, Osmolarity optimized for ES Cells Medium for neural stem cells serum free base medium and supplement for the expansion of neural progenitors Serum Free Media and Supplements Coming Soon Cell type and developmental stage specific base medium and supplements for expansion, differentiation and maintenance of stem cells and mature cell types Hepatocytes Expansion of immature diploid liver cells Maintenance of mature hepatocytes Mesenchymal Stem Cells (MSCs from bone marrow, adipose tissue and umbilical cord matrix) Expansion Medium for the MSCs Adipocyte Differentiation Medium Adipocyte Maintenance Medium Human Embryonic Stem Cell Medium Human Neural Stem Cells Mouse Neural Stem Cells Rat Neural Stem Cells Murine Embryonic Stem Cell Lines Germline competent, Karyotyped, Mycoplasma and MAP Tested C57BL6 DBA/1 129 SvEv Primary Murine Embryo Fibroblasts For use as feeder layer for Murine and Human ES Cells CF-1 PMEF growth arrested with Mitomycin-C passage 3, 5 X 10 6 cells per vial CF-1 PMEF untreated, passage 3, 5 X 10 6 cells per vial Neomycin resistant growth arrested with Mitomycin-C passage 3, 5 X 10 6 cells per vial Neomycin resistant untreated, passage 3, 5 X 10 6 cells per vial US/Canada: International: Fax: sales@zenithbiotech

7 zenith biotech Custom Services Scientists at Zenith have extensive experience in all aspects of stem cell technology from formulating media for embryo and stem cell culture to isolating stem cells and developing a cell-based assay for a high throughput screen (HTS). Our products and services are designed to make it possible for stem cells to become a platform technology throughout the research community and pharmaceutical industry. If you don t find what you need in our catalog, we will partner with you to develop and produce a custom media, a new cell line or cell type, or a cellular model for your specific applications, including optimizing a product already in our catalog. If you need it, we will develop and produce it. Services currently offered at Zenith Custom Media Formulate media for your specific cell type Produce media from your formulation Optimize a media for your application Embryonic Stem Cells Develop a new ES cell Line from mouse and rabbit Expand your ES cell line Sub-clone your ES cell line Adult Stem Cells Isolation of stem cells from adult tissues Stem Cell Assay and Model Development Develop an assay or model system optimized for your specific applications using your stem cells or ours Consulting Services We provide consulting services with the products we offer We offer consulting services for stem cell technology

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