FSS-i 3. DNA Profiling. DNA Profiling. DNA expert system suite. Sample e.g. hair, skin. Sample e.g. hair, skin. Process sample to obtain raw data
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1 FSS-i 3 DNA expert system suite Sample e.g. hair, skin DNA Profiling Sample e.g. hair, skin DNA Profiling Process sample to obtain raw data 1
2 Sample e.g. hair, skin DNA Profiling Process sample to obtain raw data Produce a profile of the DNA Sample e.g. hair, skin DNA Profiling Process sample to obtain raw data Produce a profile of the DNA Store profile in NDIS (National DNA Index System) or match to existing profile Expert System profiling motivation Sample & data processing already heavily automated 2
3 Expert System profiling motivation Sample & data processing already heavily automated Sample data analysis; DNA profiling is the bottle neck. Expert System profiling motivation Sample & data processing already heavily automated Sample data analysis; DNA profiling is the bottle neck. Two independent data reviews required to upload a profile to NDIS Expert System profiling motivation Sample & data processing already heavily automated Sample data analysis; DNA profiling is the bottle neck. Two independent data reviews required to upload a profile to NDIS Save enormous amounts of review time 3
4 FSS-i 3 Expert System FSS: Forensic Science Service of the UK FSS-i 3 Expert System FSS: Forensic Science Service of the UK Developed a software suit called FSS-i 3 6 years of R&D and validation FSS-i 3 Expert System FSS: Forensic Science Service of the UK Developed a software suit called FSS-i 3 6 years of R&D and validation Designed by forensic scientists for forensic scientists 4
5 FSS-i 3 Expert System FSS: Forensic Science Service of the UK Developed a software suit called FSS-i 3 6 years of R&D and validation Designed by forensic scientists for forensic scientists Made available to forensic community USA, Canada, Europe, Australia, New Zealand FSS-i 3 Expert System FSS: Forensic Science Service of the UK Developed a software suit called FSS-i 3 6 years of R&D and validation Designed by forensic scientists for forensic scientists Made available to forensic community USA, Canada, Europe, Australia, New Zealand Full Audit system i-stress The core DNA interpretation tool 5
6 i-stress The core DNA interpretation tool Produces single source DNA profiles using configurable rules & filters i-stress The core DNA interpretation tool Produces single source DNA profiles using configurable rules & filters Detect multi-person DNA mixtures within a profile & export to i-stream i-stream Unique functionality to resolve two person DNA mixtures 6
7 i-stream Unique functionality to resolve two person DNA mixtures Allows previously unusable DNA profiles to be uploaded to DNA databases i-ntegrity DNA samples/data can become contaminated: accidental DNA mixture i-ntegrity DNA samples/data can become contaminated: accidental DNA mixture System flags possible profile contaminations for further review 7
8 i-ntegrity DNA samples/data can become contaminated: accidental DNA mixture System flags possible profile contaminations for further review Saves time: no more manual reviews of profiles FSS-i 3 Validation FSS-i 3 developed from internal FSS expert systems 6 years of testing FSS-i 3 Validation FSS-i 3 developed from internal FSS expert systems 6 years of testing 1 million+ profiles uploaded to UK National DNA DB 8
9 FSS-i 3 Validation FSS-i 3 developed from internal FSS expert systems 6 years of testing 1 million+ profiles uploaded to UK National DNA DB FSS-i 3 validation project: previously analyzed samples Minor discrepancies in 0.03% of samples & each of these samples were flagged by the system for further review Technology used in FSS-i 3 Strategies for implementing rules: unknown Technology used in FSS-i 3 Strategies for implementing rules: unknown User optimization: 20+ rules settings 9
10 Technology used in FSS-i 3 Strategies for implementing rules: unknown User optimization: 20+ rules settings Designed to run on stand-alone Pentium 3 PC s Design & Development of FSS-i 3 Development time: six years Design & Development of FSS-i 3 Development time: six years The FSS developers who designed the FSS-i3 suite are, first and foremost, DNA scientists who understand the problems forensic scientists face (1) 10
11 Design & Development of FSS-i 3 Development time: six years The FSS developers who designed the FSS-i3 suite are, first and foremost, DNA scientists who understand the problems forensic scientists face (1) i-stream is based on scientific theory developed by Dr Peter Gill and Dr Tim Clayton (1) Conclusions: FSS-i 3 Consistent profiling of samples Conclusions: FSS-i 3 Consistent profiling of samples Ability to interpret mixed DNA profiles 11
12 Conclusions: FSS-i 3 Consistent profiling of samples Ability to interpret mixed DNA profiles Check for contamination Conclusions: FSS-i 3 Consistent profiling of samples Ability to interpret mixed DNA profiles Check for contamination Significant reduction in time spent on the above, due to automation References (1) (2) (3) rtsys.pdf (4) (5) (6) 12
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