hplc applications nutraceuticals vitamins Ginsenosides in Korean Ginseng Ginseneoside ww.discoverysciences.com Ginseng
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1 Ginseng Ginsenosides in Korean Ginseng Rh. Rh. Rg. Rg. Rg. Rf 7. Re 8. Rd 9. Rc. Rb. Rb Min. Column: Alltech Prevail Carbohydrate ES, µm, x.mm (Part No. ) Mobile Phase: A: Acetonitrile:Water:Isopropyl Alcohol (8::) B: Acetonitrile:Water:Isopropyl Alcohol (7::) Gradient: Time: 8 7 %B: Flow Rate:.8mL/min Min. Competitor C8 Competitor C8 Competitor C8 Ginseneoside. Rg. Re. Rb 7 Competitor C8 Competitor C8 GraceSmart C8 Column: GraceSmart C8, µm, x.mm (Part No. 88) Mobile Phase: A: Water B: Acetonitrile Gradient: Time: 7 %B: Ginkgo Biloba Folium Ginkgo Terpene Lactones High Throughput Ginkgolide C. Bilobalide. Ginkgolide A. Ginkgolide B. Bilobalide. Ginkgolide J. Ginkgolide C. Ginkgolide A. Ginkgolide B Min. Column: GraceSmart C8, µm, x.mm (Par t No. 88) Mobile Phase: Methanol:Tetrahydrofuran:Water (::) Column: Alltech Alltima C8, µm, x 7mm Rocket (Part No. ) Mobile Phase: A:.% Trifluoroacetic Acid in Water B:.% Trifluoroacetic Acid in Methanol Gradient: Time: %B: Flow Rate:.mL/min Ginkgo Biloba Flavonol Glycoside Standards Ginkgo Biloba Terpene Lactone Standards Quercetin. Kaempferol. Isorhamnetin. Bilobalide. Ginkgolide J. Ginkgolide C. Ginkgolide A. Ginkgolide B Column: Alltech Platinum C8, µm, x.mm (Part No. ) Mobile Phase: A:.% Trifluoroacetic Acid in Water:Methanol (9:) B:.% Trifluoroacetic Acid in Methanol Gradient: Time: %B: 9 Column: Alltech Alltima C8, µm, x.mm (Part No. 88) Mobile Phase: A:.% Trifluoroacetic Acid in Water B:.% Trifluoroacetic Acid in Methanol Gradient: Time: %B: Flow Rate:.mL/min more applications View our complete searchable chromatogram database at ww.discoverysciences.com
2 Liquid Ginkgo Biloba Extract Prep Min.. Bilobalide. Ginkgolide J. Ginkgolide C. Ginkgolide A. Ginkgolide B Column: Alltech Alltima C8, µm, x mm (Part No. 8) Mobile Phase: A:.% Trifluoroacetic Acid in Water B:.% Trifluoroacetic Acid in Methanol Gradient: Time: %B: Flow Rate:.mL/min Flavonoids Polyphenolic Flavonoids. Myricetin. Quercetin. Naringenin. Hesperetin. Apigenin. Kaempferol 997 Column: Alltech Prevail C8, µm, x.mm (Part No. 998) Mobile Phase: A:.M KHPO, ph. B: Acetonitrile Gradient: Time: %B: Detector: UV at 8 Flavonoid Mix. Myricetin. Quercetin. Apigenin. Kaempferol Flavonoid Mix Naringin. Neohesperidin. Myricetin. Quercetin. Naringenin. Kaempferol Column: Mobile Phase: Flow Rate: Detector: Min. Alltech Alltima C8 µm, x 7mm Rocket (Part No. ).% Trifluoroacetic Acid in Water:.% Trifluoroacetic Acid in Acetonitrile (:).ml/min ELSD Column: Alltech Alltima C8, µm, x.mm (Part No. 88) Mobile Phase: A:.M KHPO, ph. B: Acetonitrile Gradient: Time: %B: Detector: UV at 8nm St. John s Wort Echinacea Hypericins. Pseudohypericin. Hypericin 97 9 Phenolic Acids. Protocatechuic Acid. Chlorogenic Acid. Caffeic Acid Column: Alltech Alltima C8, µm, x 7mm Rocket (Part No. ) Mobile Phase:.% Phosphoric Acid:Methanol (:9) Flow Rate:.mL/min Detector: Vis at 8nm Column: Alltech Alltima C8, µm, x 7mm Rocket (Part No. ) Mobile Phase:.M KHPO, ph.:acetonitrile (8:) Flow Rate:.mL/min Detector: UV at nm
3 Chinese Pharmacopeia Fructus Liquidambaris Fructus Kochiae 7 7. Liguidanbaric Acid. Momordin lc 8 8 Min Min. Column: GraceSmart C8, µm, x.mm (Par t No. 88) Column: GraceSmart C8, µm, x.mm (Part No. 88) Mobile Phase: Methanol:Water:Acetic Acid (87::.) Mobile Phase: Methanol:Water:Acetic Acid (8::.) Bulbus Fritillariae Thunbergii 7 Rhizoma Anemarrhenae 7. Peimine. Peininine. Sarsasapogenin 8 Min. Column: GraceSmart C8, µm, x.mm (Part No. 88) Mobile Phase: Acetonitrile:Water:Diethylamine (7::.) 8 Min. Column: GraceSmart C8, µm, x.mm (Part No. 88) Mobile Phase: Methanol:Water (9:) Radix Astragali 77. Astragaloside IV 8 Min. Column: GraceSmart C8, µm, x.mm (Part No. 88) Mobile Phase: Acetonitrile:Water (:) more applications View our complete searchable chromatogram database at ww.discoverysciences.com
4 mau Puerarin Min. Competitor C8 Competitor C8 Competitor C8 Puerarin. Puerarin 77 Competitor C8 Competitor C8 GraceSmart C8 Column: GraceSmart C8, µm, x.mm (Part No. 88) Mobile Phase: Methanol:.% Citric Acid (:7) Detector: UV at nm mau Tanshinone II Min. Competitor C8 Competitor C8 Competitor C8 Tanshinone II. Tanshinone II 79 Competitor C8 Competitor C8 GraceSmart C8 Column: GraceSmart C8, µm, x.mm (Part No. 88) Mobile Phase: Methanol:Water (7:) Detector: UV at 7nm Green Tea Green Tea Extract - Analytical Green Tea Extract - Preparative 9. Caffeine. Epicatechin. Epigallocatechin Gallate. Caffeine. Epicatechin. Epigallocatechin Gallate 8 8 Min. Column: Alltech Alltima C8, µm, x.mm (Part No. 88) Mobile Phase:.% Trifluoroacetic Acid:Acetonitrile (87:) Column: Alltech Alltima C8, µm, x mm (Part No. 8) Mobile Phase:.% Trifluoroacetic Acid:Acetonitrile (87:) Flow Rate: ml/min Catechins Gallic Acid. Epigallocatechin. Catechin. Epicatechin. Epigallocatechin Gallate. Caffeine 7. Gallocatechin 8. Epicatechin Gallate Catechins and Gallic Acid in Green Tea. Gallic Acid. Gallocatechin. Epigallocatechin. Catechin. Epicatechin. Epigallocatechin Gallate 7. Gallocatechin Gallate 8. Epicatechin Gallate 9. Catechin Gallate Column: Alltech Platinum EPS C8,.µm, x 7mm Rocket (Part No. 77) Mobile Phase:.% Trifluoroacetic Acid:Acetonitrile (87:) Flow Rate:.mL/min Detector: UV at nm Min. Column: Vydac Denali, µm, x.mm (Part No. 8DE) Mobile Phase: A: Acetonitrile:mM Phosphoric Acid (:9) B: Acetonitrile:mM Phosphoric Acid (9:) Gradient: Time: B%: Detector: UV at 8nm
5 Vitamins Water- and Fat-Soluble Vitamins 9. Thiamine. Ascorbic Acid. Nicotinic Acid. Niacinamide. Pantothenic Acid. Riboflavin 7. Cyanocobalamin 8. Biotin 9. Vitamin A. -Tocopherol. -Tocopherol. -Tocopherol. Vitamin D. Vitamin D. Vitamin K Column: Alltech Prevail C8, µm, x.mm (Part No. 998) Mobile Phase: A: Water ph. w/formic Acid B: Acetonitrile:Methanol (8:7) Gradient: Time:. %B: Flow Rate:.mL/min Fat-Soluble Vitamins and Tocopherols 99. Vitamin A. -Tocopherol. -Tocopherol. -Tocopherol (Vitamin E). Vitamin D. Vitamin D 7. Vitamin K Water-Soluble Vitamins 7. Ascorbic Acid. Nicotinic Acid. Pyridoxamine. Niacinamide. Folic Acid. Vitamin B 7. Riboflavin 8 Min. Column: GraceSmart C8, µm, x.mm (Part No. 88) Mobile Phase: A:.M Ammonium Dihydrogen Orthophosphate Buffer, ph. B: Acetonitrile Gradient: Time: B%: Detector: UV at nm Fat-Soluble Vitamins. Vitamin A (ng on column). Vitamin E (ng on column). Vitamin K (ng on column) 7 79 Column: Alltech Prevail C8, µm, x.mm (Part No. 998) Mobile Phase: Acetonitrile:Methanol (7:) Flow Rate:.mL/min Detector: UV at nm Min. Column: Alltech Alltima C8, µm, x mm (Part No. 888) Mobile Phase: Methanol:Chloroform (9:) Flow Rate:.mL/min Water-Soluble Vitamins from Multi-Vitamin Tablet 7. Thiamine. Ascorbic Acid. Unknown. Fumaric Acid. Pyridoxine. Niacinamide 7. Pantothenic Acid 9 Water-Soluble Vitamins 7. Ascorbic Acid. Nicotinic Acid. Pyridoxamine. Niacinamide. Folic Acid. Riboflavin 7. Vitamin B 8 8 Min. Column: Alltech Prevail C8, µm, x.mm (Part No. 998) Mobile Phase:.M KHPO, ph.:acetonitrile (97:) Detector: UV at 8 Min. Column: Alltech Platinum C8, µm, x.mm (Part No. ) Mobile Phase: A:.M Ammonium Phosphate, ph. B: Acetonitrile Gradient: Time: 8 B%: Detector: UV at nm more applications View our complete searchable chromatogram database at ww.discoverysciences.com
6 Vitamins Analysis of Tocopherols Vitamin E. -Tocopherol. -Tocopherol. -Tocopherol. -Tocopherol Vitamin E Min. Column: Grom Sil Amino- PA, µm, x mm Column: Jones Genesis Si, µm, x.mm (Part No. ) (Part No. FM9E) Mobile Phase: Hexane:Ethyl Acetate (7:) Mobile Phase: Hexane:,-Dioxane (9:) Flow Rate:.8mL/min Flow Rate:.mL/min Detector: UV at 9nm Detector: Fluorescence at 9nm (exc.), nm (em.) Vitamin E Soft Gel Tablet. -Tocopherol. -Tocopherol. -Tocopherol 98 Column: Alltech Prevail C8, µm, x.mm (Part No. 998) Mobile Phase: Acetonitrile:Methanol (7:) Flow Rate:.mL/min Column: Vydac C, µm, x.mm (Part No. TP) Mobile Phase: A: Buffer:Methanol:n-Butanol (:7:) B: Buffer:Methanol:n-Butanol (:88:) Buffer:Aqueous.M Ammonium Acetate, ph. Gradient: Time: 8 %B: Flow Rate:.mL/min Detector: Absorbance, programmed wavelength. -Tocopherol. -Tocotrienol. -Tocopherol. -Tocopherol. -Tocotrienol. -Tocotrienol 7. -Tocotrienol 8. -Tocotrienol Determination of Retinol, -Tocopherol, and -Carotene in Serum. All-trans-Retinol. Tocol. -Tocopherol. -Tocopherol. Lutein. Zeaxanthin 7. Cryptoxanthin 8. -Carotene 9. All-trans--Carotene. cis--carotene First Step Separation of Tocopherol -Isomer Preparative Purification of Tocopheral Rechromatography of -Isomer-Enriched Tocopherol 8 Min. 8 Min. Shaded area indicates collected fraction. Shaded area indicates collected fractions. Sample is g tocopherol isomer mixture in ml hexane. Sample is pooled fraction from first step. Silica: Davisil LCÅ -µm, (Part No. 9) Silica: Davisil LCÅ -µm, (Part No. 9) Column: x mm Column: x mm Mobile Phase: Hexane:.% Tetrahydrofuran Flow Rate: ml/min Flow Rate: ml/min
7 Carotenes Coenzyme Q. -Carotene. cis--carotene. -Carotene. -cis--carotene. 9-cis--Carotene Column: Vydac C, µm, x.mm (Part No. TP) Mobile Phase: Methanol:Chloroform (9:) mau 8 Min. Competitor C8 Competitor C8 Competitor C8. Coenzyme Q 77 Competitor C8 Competitor C8 GraceSmart C8 Column: GraceSmart C8, µm, x.mm (Par t No. 88) Mobile Phase: Methanol:Ethanol (:) Detector: UV at 7nm Devil s Claw Iridoid Compounds 99 Saponins HPLC Analysis of Saikosides. 8-para-Coumaroyl Harpagoside. Harpagoside. Saikosaponin c. Saikosaponin f. Saikosaponin a. Saikosaponin b. Saikosaponin b. Saikosaponin d Column: Alltech Alltima C8, µm, x 7mm Rocket (Part No. ) Mobile Phase:.% Trifluoroacetic Acid:Methanol:Dioxane (7::) Flow Rate:.mL/min Detector: UV at 8nm 8 8 Min. Column: Grom Sil ODS- CP, µm, x mm Mobile Phase: Water:Methanol:Acetonitrile (::) Flow Rate:.8mL/min Detector: UV at nm more applications View our complete searchable chromatogram database at ww.discoverysciences.com 7
8 Goldenseal Root Alkaloids 9 Black Cohosh Black Cohosh Extract 99. Hydrastine. Berberine Column: Alltech Alltima C8, µm, x 7mm Rocket (Part No. ) Mobile Phase:.M KHPO, ph.:methanol (:8) Flow Rate:.mL/min Detector: UV at nm. Cimiracemoside A. Actein. 7-deoxyactein Column: Alltech Prevail C8, µm, x.mm (Part No. 99) Mobile Phase: A: % Formic Acid B: Acetonitrile Gradient: Time: 7 8 %B: Glucosamine Glucosamine Evening Primrose Triglycerides UV ELSD RI. Glucosamine 7 8. LGG. LGL. LLL. OGL. PGL. OLL 7. OGO + PLL 8. OOO 7 Min. Min. Column: Alltech Prevail Carbohydrate ES, µm, x.mm (Part No. ) Mobile Phase: Acetonitrile:Water (:) Column: Alltech Alltima C8, µm, x.mm (Part No. 88) Mobile Phase: A: Acetonitrile B: Dichloromethane Gradient: Time: %B: 8
Column: Alltech Prevail Carbohydrate ES, 5µm, 250 x 4.6mm. Mono-, Di-, Tri- Saccharides
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