HPLC Applications. Analgesics Anti-Inflammatories. 1. Fenoprofen 2. Flurbiprofen 3. Ketoprofen. 1. Naproxen 2. Acemetacin 3. Indomethacin 4.

Transcription

1 HPLC Applications P H A R M A C E U T I C A L S Analgesics Anti-Inflammatories. Naproxen. Acemetacin. Indomethacin. p-xylene 8 Anti-Inflammatories. Fenoprofen. Flurbiprofen. Ketoprofen 08 Column: Platinum C8, µm, 0 x.mm (Part No. 0) Mobile Phase: 0.0M KHPO:Methanol:Acetonitrile: ph. (0:0:0) Detector: UV at nm Column: Inertsil Diol, µm, 0 x.mm (Part No. GL-0) Mobile Phase: Hexane:Ethanol (90:0) Detector: UV at Analgesics 0 Analgesics 98. Aspirin. Acetaminophen. Naproxen. Fenprofen. Ibuprofen. Diclofenac. Aspirin. Acetaminophen. Naproxen. Fenoprofen Column: Alltima C8, µm, 0 x.mm (Part No. 880) Mobile Phase: A: 0.0M KHPO ph. B: Methanol C: Acetonitrile Gradient: Time: 0 %B: %C: 0 0 Flow Rate:.0mL/min Detector: UV at 80nm Column: Prevail Amide, µm, 0 x.mm (Part No. 880) Mobile Phase: 0.0M KHPO ph.0:acetonitrile (:) Detector: UV at 0nm Basic Drugs 0 Naproxen 89. Procainimide. Acetaminophen. Theophylline. Caffeine CH CHCOH H CO O Min. Column: Alltima HP C8, µm, 0 x.mm (Part No. 8) Mobile Phase: 0.0M Phosphate Buffer ph.0:methanol (80:0) Detector: UV at nm Copyright 00 Alltech Associates, Inc. Column: Chirobiotic V, 0 x.mm (Part No. 0) Mobile Phase: 0.% Triethylammonium Acetate (TEAA), ph:thf (90:0) Detector: UV at nm Contact your Alltech office or distributor for current or local prices.

2 P H A R M A C E U T I C A L S HPLC Applications Anti-Depressants Tricyclic Amines 90 Alprazolam and Triazolam by the USP XXII Method 8. Fluphenazine HCL. Chlorpromazine HCL. Phenothiazine HCL. Triazolam. Alprazolam 0 Column: Alltima CN, µm, 0 x.mm (Part No. 889) Mobile Phase: 0.0M KHPO ph.:acetonitrile (0:0) Detector: UV at nm 8 Min. Basic Drugs. Diphenhydramine. Nortriptyline. Amitriptyline Column: Alltima Silica, µm, 00 x.mm (Custom) Mobile Phase: CHCN:CHCl:i-BuOH:Water:Glacial HOAc (8:8:::0.0) Flow Rate:.0mL/min Detector: UV at nm Tricyclic Antidepressants 0 0. Desipramine. Nortryptyline. Diphenhydramine. Amitriptyline Column: Alltima HP C8 Amide, µm, 0 x.mm (Part No. 8) Mobile Phase: 0.0M Phosphate Buffer ph:methanol (:8) Flow Rate: ml/min Detector: UV at nm Min. Column: Alltima HP C8, µm, 0 x.mm (Part No. 8) Mobile Phase: A: 0.0M Phosphate Buffer ph B: Methanol Gradient: Time %B 0 Detector: UV at nm Benzodiazepines and Metabolites. Chlordiazepoxide degradant. Nitrazepam. Norchlordiazepoxide. Nordiazepam 89. Chlordiazepoxide. Chlordiazepoxide degradant. Diazepam Universal Combinatorial High Speed Gradient Application 89. Theophylline. Acetylsalicylic Acid. Propranolol. Nortriptyline. Trimipramine Column: Platinum EPS C8, µm, x mm Rocket (Part No. 0) Mobile Phase: 0.0M Ammonium Acetate ph.:acetonitrile (:) Flow Rate:.0mL/min Detector: UV at nm 0 Column: Alltima C8, µm, x mm Rocket (Part No. 00) Mobile Phase: A: 0.% TFA in Water B: 0.% TFA in Acetonitrile Gradient: Time: 0 %B: 0 90 Flow Rate:.mL/min Copyright 00 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. Min. 8

3 HPLC Applications P H A R M A C E U T I C A L S Anti-Asthmatics Methylxanthines Theophylline & Metabolites Theobromine. Xanthine. Theophylline. Theobromine. 8-Chlorotheophylline. Theophylline. Caffeine. Caffeine. -(-Chloroethyl). 8-Chlorotheophylline theophylline. -(-chloroethyl)theophylline Min. 0 Min. Column: Platinum EPS C8, 00Å, µm, 0 x.mm (Part No. ) Mobile Phase: Water:Acetonitrile:Methanol: Glacial Acetic Acid (0:0:0:) Detector: UV at nm Column: Platinum EPS C8, µm, x mm Rocket (Part No. 0) Mobile Phase: 0.00M Sodium Acetate ph.0:methanol (0:0) Flow Rate:.0mL/min Detector: UV at nm Barbiturates Barbiturate Mix 0 Barbiturate Mix 88. Barbital. Phenobarbital. Talbutal. Amobarbital. Mephobarbital 8. Barbital. Butalbarbital. Aprobarbital. Talbutal. Butabarbital. Phenobarbital. Amobarbital 8. Mephobarbital Min. 0 Min Column: Alltima HP C8, µm, 0 x.mm (Part No. 8) Mobile Phase: 0.0M Ammonium Acetate:Acetonitrile (8:) Detector: UV at 0nm Column: Platinum EPS C8, µm, x mm Rocket (Part No. 0) Mobile Phase: 0.0M Sodium Acetate ph.0:acetonitrile (:) Flow Rate:.0mL/min Detector: UV at 0nm Sedatives Sedatives/Hypnotics Anesthetic Anesthetic Mix Barbital. Phenobarbital. Butalbital. Oxazepam. Triazolam. p-aminobenzoic Acid. Benzocaine. Tetracaine. Dibucaine. Butacaine 8 Column: Platinum C8, µm, 0 x.mm (Part No. 0) Mobile Phase: 0.0M NHHPO ph. Methanol:Acetonitrile (0:0:0) Detector: UV at 0nm Copyright 00 Alltech Associates, Inc. Column: Alltima C8, µm, 0 x.mm (Part No. 880) Mobile Phase: A: Water B: Methanol C: Acetonitrile Gradient: Time: 0 %B: %C: Flow Rate:.mL/min Detector: UV at 0nm Contact your Alltech office or distributor for current or local prices.

4 P H A R M A C E U T I C A L S HPLC Applications Antihistamines Antihistamines. Tripelennamine. Triprolidine. Cyclizine. Chlorcyclizine. Meclizine Antihistamines 0. Tripelennamine. Triprolidine. Cyclizine. Pyrrobutamine Phosphate. Meclizine Min. Column: Alltima C8, µm, 0 x.mm (Part No. 880) Mobile Phase: A: 0.0M Potassium Phosphate, ph. B: Acetonitrile Gradient: Time: 0 0 %B: 0 0 Flow Rate:.mL/min Detector: UV at 0nm Column: ZirChrom PS, µm, 0 x.mm (Part No. ZR09-0) Mobile Phase: A: Acetonitrile B: mm Hydrochloric Acid Gradient: Time: %B: Detector: UV at Very Polar Bases 09. Maleate. Phenylephrine. Phenylpropanolamine. Pyrilamine. Chlorpheniramine Basic Antihistamines at High ph 0. Maleate. Scopolamine. Pseudoephedrine. Doxylamine. Chlorpheniramine. Triprolidine. Diphenhydramine Column: ZORBAX SB-Aq, µm, 0 x.mm (Part No ) Mobile Phase: 0.% TFA:Acetonitrile (90:0) Flow Rate:. ml/min Column Temp: C Detector: UV at nm 0 0 Min. Column: ZORBAX Extend-C8, µm, 0 x.mm (Part No. 0-90) Mobile Phase: 0.0M Triethylamine:Methanol (0:0) Detector: UV at nm Basic Compounds in Mid ph Range 09. Doxylamine. Chlorpheniramine. Triprolidine OTC Antihistamine and Excipients. Diphenhydramine. PEG, Gelatin. Sorbitan. Glycerol. Sorbitol 0 Column: ZORBAX Bonus-RP, µm, 0 x.mm Column: Prevail Carbohydrate ES, µm, 0 x.mm (Part No ) (Part No. 0) Mobile Phase: 0.0M Ammonium Acetate ph.:acetonitrile (80:0) Mobile Phase: Acetonitrile:0.% Ammonium Carbonate Flow Rate:.mL/min ph. (80:0) Column Temp: 0 C Detector: UV at nm Copyright 00 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 8

5 HPLC Applications P H A R M A C E U T I C A L S Anticonvulsants Anticonvulsants Flavonoids Ethotoin. Mephenytoin. Carbamazepine. Diazepam. Primidone. Metharbital. Mephenytoin. Phenobarbital. Phenytoin 0 8 Min. Column: Prevail Phenyl, µm, 0 x.mm (Part No. 999) Mobile Phase: A: Water B: Acetonitrile Gradient: Time: 0 %B: 0 Flow Rate:.mL/min Detector: UV at Column: ZirChrom DiamondBond -C8, µm, 00 x.mm (Part No. DB0-0 ) Mobile Phase: 0.0M Ammonium Phosphate ph.0:thf (:) Detector: UV at 0 Anticonvulsants. Ethotoin. Phenytoin. Mephenytoin. Carbamazepine Anticonvulsants. Ethotoin. Phenytoin. Mephenytoin. Carbamazepine Column: Alltima HP EPS C8, µm, 0 x.mm (Part No. 8) Mobile Phase: 0.0M Ammonium Acetate:Acetonitrile (0:0) Column: Alltima HP C8 HiLoad, µm, 0 x.mm (Part No. 89) Mobile Phase: 0.0M Ammonium Acetate:Acetonitrile (0:0) Amphetamines MDA & MDMA Analogs.,-MDA.,-MDA.,-MDMA.,-MDMA MDA and MDMA Analogs 00 0.,-MDA.,-MDA.,-MDMA.,-MDMA 8 Column: Alltima Silica, µm, 00 x.mm (Part No. 80) Mobile Phase: %NHOH in Methanol:Acetonitrile (:) Flow Rate:.0mL/min Detector: UV at 80nm Copyright 00 Alltech Associates, Inc Min. Column: Alltima HP Silica, µm, 0 x.mm (Part No. 88) Mobile Phase: %NHOH in Methanol:Acetonitrile(:) Column Temp: C Detector: UV at 80nm Contact your Alltech office or distributor for current or local prices.

6 P H A R M A C E U T I C A L S HPLC Applications Anti-Hypertensives Antihypertensives. Metoprolol. Oxprenolol. Propranolol 99 Basic Drugs. Labetalol. Acebutolol. Oxprenolol. Lidocaine. Alprenolol. Propranolol 0 0 Min. Column: Prevail C8, µm, x mm Part No. (999) Mobile Phase: 0.0M KHPO, ph.0:acetonitrile (0:0) Flow Rate:.0mL/min Detector: UV at 0 Column: ZirChrom PBD, µm, 0 x.mm (Part No. ZR0-) Mobile Phase: 0.0M Triethylamine, 0.0M Tetramethylammonium Hydroxide ph.:acetonitrile (:) Detection: UV at Calcium Channel Blockers 0 Drug Mix 0. Diltiazem. Verapamil. Nicardipine. Nifedipine. Codeine. Strychnine. Quinine. Quinidine Min. Column: Alltima HP CN, µm, 0 x.mm (Part No. 89) Mobile Phase 0.0M KHPO ph.0:acetonitrile (0:0) Detector: UV at 8nm Column: Alltima HP C8 Amide, µm, 0 x.mm (Part No. 8) Mobile Phase: 0.0M Potassium Phosphate ph.8:methanol (90:0) Detector: UV at nm Opioids. Naloxone. Codeine. Ethylmorphine. Oxycodone 0 Morphines & Metabolites 90. Normorphine. Morphine--Glucuronide. Morphine--Glucuronide. Morphine. Codeine Column: ZirChrom DiamondBond-C8, µm, 00 x.mm Column: Hypercarb, µm, 00 x.mm (Part No. DB0-0) (Part No. 980) Mobile Phase: 0.0M Ammonium Phosphate ph.0: Mobile Phase: Ammonium Acetate ph 9.0:Methanol (0:0) Tetrahydrofuran (.:.) Detector: UV at 0 Detector: UV at 0 Copyright 00 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 8

7 HPLC Applications P H A R M A C E U T I C A L S Steroids Steroids Steroids Hydrocortisone. Testosterone. Progesterone. Progesterone. Corticosterone. Cortisone. Prednisone. Prednisolone 0 Min. Column: Prevail C8, µm, x mm Rocket (Part No. 999) Mobile Phase: Water:Methanol (0:90) Flow Rate:.mL/min Detector: UV at Column: Inertsil Diol, µm, 0 x.mm (Part No. GL-0) Mobile Phase: Hexane:Ethanol (8:) Column Temp: 0 C Detector: UV at Hormones 0 Corticosteroids 09. β-trenbolone. α-trenbolone. β-zearalenol. Testosterone. Zeranol. α-zearalenol. α-estradiol 8. Zearalenone 9. Diethylstilbestrol. Deoxycorticosterone Acetate. Hydrocortisone Acetate. Cortisone Acetate. Cortisone. Prednisone. Hydrocortisone. Prednisolone Min. Column: Inertsil ODS-, µm, 0 x.mm (Part No. GL-0) Mobile Phase: Water:Acetonitrile:THF(::8) Column Temp: 0 C Detector: UV at 0 Column: Alltima HP CN, µm, 0 x.mm (Part No. 89) Mobile Phase: Hexane:Isopropanol (9.:.) Flow Rate:.0 ml/min Column Temp: C Detector: UV at nm Hydrocortisone Acetate Cream 9 Estropipate Sample 0. Methyl Paraben. Propyl Paraben. Hydrocortisone Acetate. Estrone Sulfate. Piperazine 88 Column: Prevail C8, µm, 0 x.mm (Part No. 9908) Mobile Phase: Water:Acetonitrile (0:90) Detector: UV at Copyright 00 Alltech Associates, Inc. Column: Alltima CN, µm, 0 x.mm (Part No. 8889) Mobile Phase: 0.% HNO:Acetonitrile (:9) Flow Rate: 0.mL/min Contact your Alltech office or distributor for current or local prices.

8 P H A R M A C E U T I C A L S Diuretics 0 Column: Alltima C8, µm, 0 x.mm (Part No. 880) Mobile Phase: A: 0.% TFA in 0.0M Ammonium Acetate B: 0.% TFA in Acetonitrile Gradient: Time 0 0 %B Detector: UV at nm Min. 8 9 HPLC Applications Diuretics and Uricosuric Agents. Amiloride HCl Hydrate. Acetazolamide. Hydrochlorothiazide. Triamterene. Chlorthalidone. Furosemide. Hydroflumethiazide 8. Clopamide 9. Ethacrynic Acid 0. Trichloromethiazide. Probenecid. Indapamide. Bumetanide. Bendroflumethiazide. Spironolactone Doping Agents. Amiloride HCl Hydrate. Acetazolamide. Hydrochlorothiazide. Chlorthalidone. Hydroflumethiazide. Clopamide. Trichloromethiazide 8. Indapamide 9. Bendroflumethiazide 0. Bumetanide. Probenecid. Spironolactone Min. Column: Alltima C8, µm, 0 x.mm (Part No. 880) Mobile Phase: A: 0.% TFA in 0.0M Ammonium Acetate B: 0.% TFA in Acetonitrile Gradient: Time 0 0 %B 9 9 Flow Rate:.0 ml/min 8 Diuretics - High Throughput 9. Acetazolamide. Hydrochlorothiazide. Triamterene. Hydroflumethiazide. Clopamide. Trichloromethiazide. Indapamide 8. Bumetanide 9. Spironolactone 0 0 Min. Column: Alltima C8, µm, x mm Rocket (Part No. 00) Mobile Phase: A: 0.% TFA in 0.0M Ammonium Acetate B: 0.% TFA in Acetonitrile Gradient: Time 0 %B 0 9 Flow Rate:.mL/min Detector: UV at nm Copyright 00 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 89

9 HPLC Applications P H A R M A C E U T I C A L S Antibiotics Antibacterials Antibacterials 8 8. Sulfanilimide. Oxytetracycline. Cefaclor Dihydrate. Cefatrizine. Tetracycline HCL. Thiamphenicol. Meclocycline. Cefotaxime Sulfosalicylate Salt Min. Column: Platinum EPS C8, µm, 0 x.mm (Part No. 0) Mobile Phase: 0.0M KHPO ph.0:acetonitrile (90:0) Detector: UV at 0nm Column: Alltima C8, µm, 00 x.mm (Part No. 88) Mobile Phase: A: 0.0M KHPO ph.0 B: Acetonitrile Gradient Time: 0 0 %B: 0 Detector: UV at nm Aminoglycoside Antibiotics. Streptomycin. Amikacin. Tobramycin. Neomycin Antibacterials Min Neomycin. Amoxicillin. Vancomycin. Cefatrizine. Metampacillin. Cefotaxime. Unknown 8. Bacitracin 9. Pipericillin 0. Bacampacillin. Oxacillin 99 Column: Alltima C8, µm, 0 x.mm (Part No. 880) Mobile Phase: 0.% Pentafluoropropionic Acid in.mm Ammonium Formate ph.:0.% Pentafluoropropionic Acid in Methanol (:) Column: Prevail C8, µm, 0 x.mm (Part No. 9908) Mobile Phase: A: 0.0M KHPO ph.0 B: Acetonitrile Gradient: Time: 0 %B: Detector: UV at 0 Tylosin and Monensin Sulfa Drugs Tylosin (00ppm). Monensin (80ppm). Sulfanilamide. Sulfamerazine. Sulfamethazine. Sulfamethizole 90 Column: Prevail Select C8, µm, 0 x.mm (Part No. 9900) Mobile Phase: Water ph9 w/nhoh:acetonitrile (:9) Copyright 00 Alltech Associates, Inc. Column: Alltima HP C8 HiLoad, µm, 0 x.mm (Part No. 89) Mobile Phase: 0.% Phosphoric Acid:Acetonitrile (8:) Detector: UV at nm Contact your Alltech office or distributor for current or local prices.

10 P H A R M A C E U T I C A L S HPLC Applications mau 0 0 Cephalosporins. Cephalexin. Cephaclor. Cephuroxime. Cephoxtin Min Uracil 0. Minocycline. Tetracycline. Chlortetracycline. Doxycycline Tetracyclines Column: ZORBAX Bonus-RP, µm, 0 x.mm (Part No ) Mobile Phase: 0.0M Sodium Citrate ph:methanol (:) Detector: UV at nm Column: ZORBAX Rx-C8, µm, 0 x.mm (Part No ) Mobile Phase: 0.% TFA:Acetonitrile (:) Flow Rate:.0 ml/min Detector: UV nm Antibiotics: High Speed Separation 08 Antiseptics Highly Polar Cosmetic Ingredients 0. Ceftazidime. Cefotaxime. Ciprofloxacin. Ceftazolin. Allantoin. Bronopol Column: ZORBAX SB-C8,.µm, x. mm (Part No ) Mobile Phase: 0.% TFA:Acetonitrile (9:8) Flow Rate:.0mL/min Column Temp.: 0 C Detector: UV 0nm Taxols. 0-Deacetylbaccatin III. Baccatin III. -Xylosyl-0-deacetyltaxol B Taxol and Related Taxanes in Yew. Taxinine M. -Xylosyl-0-deacetyltaxol. -Xylosyl-0-deacetyltaxol C. 0-Deacetyltaxol 8. -Xylosyltaxol 9. Cephalomannine (Taxol B) 0. -Epi-0-deacetyltaxol. Taxol. Taxol C. -Epitaxol Column: ZirChrom CARB, µm, 00 x.mm (Part No. ZR0-0) Mobile Phase: Water:Acetonitrile (80:0) Detector: UV at Deacetylbaccatin III 9. Baccatin III. -Xylosyl-0-deacetyltaxol B Taxol and Related Taxanes in Yew Taxinine M. -Xylosyl-0-deacetyltaxol. -Xylosyl-0-deacetyltaxol C. 0-Deacetyltaxol 8. -Xylosyltaxol 9. Cephalomannine (Taxol B) 0. -Epi-0-deacetyltaxol. Taxol. Taxol C. -Epitaxol Column: Alltima Phenyl µm, x mm Rocket (Part No. 8) Mobile Phase: 0.0% TFA in Water:Methanol: Acetonitrile (0:9:) Flow Rate:.mL/min Detector: UV at 0nm 0 Min. Column: Alltima CN, µm, x mm Rocket (Part No. 80) Mobile Phase: A: -Methoxy--propanol B: Acetonitrile C: Water Gradient: Time: 0 8 %B: 0 %C: Flow Rate:.mL/min Detector: UV at nm Copyright 00 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 9

11 HPLC Applications N U T R A C E U T I C A L S Ginseng Ginsenosides in Korean Ginseng. Rh. Rh. Rg. Rg. Rg. Rf. Re 8. Rd 9. Rc 0. Rb. Rb Black Cohosh 0. Cimiracemoside A 99. Actein. -deoxyactein Black Cohosh Extract Column: Prevail Carbohydrate ES, µm, 0 x.mm (Part No. 0) Mobile Phase: A: Acetonitrile:Water:Isopropyl Alcohol (80::) B: Acetonitrile:Water:Isopropyl Alcohol (::) Gradient: Time: %B: Flow Rate: 0.8mL/min Min. Column: Prevail C8, µm, 0 x.mm (Part No. 990) Mobile Phase: A: % Formic Acid B: Acetonitrile Gradient: Time: %B: Ginkgo Biloba Ginkgo Biloba Flavonol Glycoside Standards Ginkgo Biloba Terpene Lactone Standards Quercetin. Kaempferol. Isorhamnetin. Bilobalide. Ginkgolide J. Ginkgolide C. Ginkgolide A. Ginkgolide B Min. 0 Column: Platinum C8, µm, 0 x.mm (Part No. 0) Mobile Phase: A: 0.0% TFA in Water:Methanol (9:) B: 0.0% TFA in Methanol Gradient: Time 0 0 %B 0 90 Terpene Lactones High Throughput Min. Column: Alltima C8, µm, 00 x.mm (Part No. 88) Mobile Phase: A: 0.% TFA in Water B: 0.% TFA in Methanol Gradient: Time 0 %B 0 Flow Rate:.mL/min Liquid Ginkgo Biloba Extract Prep Bilobalide. Ginkgolide J. Ginkgolide C. Ginkgolide A. Ginkgolide B. Bilobalide. Ginkgolide J. Ginkgolide C. Ginkgolide A. Ginkgolide B 9 0 Column: Min. Alltima C8, µm, x mm Rocket Column: Alltima C8, µm, 0 x 0mm (Part No. 00) (Part No. 80) Mobile Phase: A: 0.% TFA in Water B: 0.% TFA in Methanol Gradient: Time 0 %B 0 Flow Rate:.0mL/min Copyright 00 Alltech Associates, Inc. Mobile Phase: A: 0.% TFA in Water B: 0.% TFA in Methanol Gradient: Time 0 %B Flow Rate:.0mL/min Contact your Alltech office or distributor for current or local prices.

12 N U T R A C E U T I C A L S HPLC Applications Flavonoids Polyphenolic Flavonoids Flavonoid Mix Myricetin. Quercetin. Naringenin. Hesperetin. Apigenin. Kaempferol. Myricetin. Quercetin. Apigenin. Kaempferol Min. 0 Min. Column: Prevail C8, µm, 0 x.mm (Part No. 9908) Mobile Phase: A: 0.0M KHPO ph. B: Acetonitrile Gradient: Time: 0 %B: Detector: UV at 80 Column: Alltima C8 µm, x mm Rocket (Part No. 00) Mobile Phase: 0.% TFA in Water:0.% TFA in Acetonitrile (:) Flow Rate:.mL/min Flavonoid Mix Flavonoid Derivatives 888. Naringin. Neohesperidin. Myricetin. Quercetin. Naringenin. Kaempferol 8. Troxerutin. Rutin. Fisetin. Morin. Luteolin. Quercetin. Kaempferol 8. Chrysin 0 Column: Alltima C8, µm, 00 x.mm (Part No. 88) Mobile Phase: A: 0.0M KHPO ph. B: Acetonitrile Gradient: Time: 0 %B: 0 0 Flow Rate: Detector:.0mL/min UV at 80nm Min. Column: Inertsil ODS-, µm, 0 x.mm (Part No. GL-0) Mobile Phase: 0.0M NaHPO ph.0:methanol:tetrahydrofuran (0:0:0) Column Temp: 0 C Detector: UV at St. John s Wort Echinacea Hypericins Phenolic Acids Pseudohypericin. Hypericin. Protocatechuic Acid. Chlorogenic Acid. Caffeic Acid 0 Min. 0 Min. Column: Alltima C8, µm, x mm Rocket Column: Alltima C8, µm, x mm Rocket (Part No. 00) (Part No. 00) Mobile Phase: 0.% Phosphoric Acid:Methanol (:9) Mobile Phase: 0.0M KHPO ph.:acetonitrile (80:0) Flow Rate:.0mL/min Flow Rate:.mL/min Detector: Vis at 8nm Detector: UV at 0nm Copyright 00 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 9

13 HPLC Applications N U T R A C E U T I C A L S Green Tea Green Tea Extract - Analytical 09 Green Tea Extract - Preparative 00. Caffeine. Epicatechin. Epigallocatechin Gallate. Caffeine. Epicatechin. Epigallocatechin Gallate Column: Alltima C8, µm, 0 x.mm (Part No. 880) Mobile Phase: 0.0% TFA:Acetonitrile (8:) Column: Alltima C8, µm, 0 x mm (Part No. 80) Mobile Phase: 0.0% TFA:Acetonitrile (8:) Flow Rate: ml/min Catechins 88. Gallic Acid. Epigallocatechin Catechin. Epicatechin. Epigallocatechin Gallate. Caffeine. Gallocatechin 8. Epicatechin Gallate Devil s Claw Iridoid Compounds para-Coumaroyl Harpagoside. Harpagoside Column: Platinum EPS C8,.µm, x mm Rocket (Part No. 0) Mobile Phase: 0.0% v/v TFA in 8: Water:Acetonitrile Flow Rate:.mL/min Detector: UV at 0nm 0 Min. Column: Alltima C8, µm, x mm Rocket (Part No. 00) Mobile Phase: 0.0% TFA:Methanol:Dioxane (::) Flow Rate:.0mL/min Detector: UV at 8nm Goldenseal Root Alkaloids 9 Evening Primrose Triglycerides 00. Hydrastine. Berberine. LGG. LGL. LLL. OGL. PGL. OLL. OGO + PLL 8. OOO 0 Min. 9 Column: Alltima C8, µm, x mm Rocket (Part No. 00) Mobile Phase: 0.0M KHPO ph.:methanol (:8) Flow Rate:.0mL/min Detector: UV at nm Copyright 00 Alltech Associates, Inc. Column: Alltima C8, µm, 0 x.mm (Part No. 880) Mobile Phase: A: Acetonitrile B: Dichloromethane Gradient: Time: 0 0 %B: 0 0 Contact your Alltech office or distributor for current or local prices.

14 N U T R A C E U T I C A L S HPLC Applications Vitamins Min. Column: Prevail C8, µm, 0 x.mm (Part No. 9908) Mobile Phase: A: Water ph. w/formic Acid B: Acetonitrile:Methanol (8:) Gradient: Time %B Flow Rate: Detector: 8 Water- and Fat-Soluble Vitamins 9.mL/min ELSD 0 Fat-Soluble Vitamins and Tocopherols 9. Thiamine. Ascorbic acid. Nicotinic acid. Niacinamide. Pantothenic acid. Riboflavin. Cyanocobalamin 8. Biotin 9. Vitamin A 0. Δ-Tocopherol. γ-tocopherol. α-tocopherol. Vitamin D. Vitamin D. Vitamin K 990. Vitamin A. Δ-Tocopherol. γ-tocopherol. α-tocopherol (Vitamin E). Vitamin D. Vitamin D. Vitamin K. Vitamin A (00ng on column). Vitamin E (0ng on column). Vitamin K (0ng on column) Column: Alltima C8, µm, 0 x mm (Part No. 888) Mobile Phase: Methanol:Chloroform (90:0) Flow Rate: 0.0mL/min Fat-Soluble Vitamins Vitamin E Soft Gel Tablet. Δ-Tocopherol. γ-tocopherol. α-tocopherol Min. 0 9 Min. Column: Prevail C8, µm, 0 x.mm (Part No. 9908) Mobile Phase: Acetonitrile:Methanol (:) Flow Rate:.mL/min Detector: UV at 0 Column: Prevail C8, µm, 0 x.mm (Part No. 9908) Mobile Phase: Acetonitrile:Methanol (:) Flow Rate:.mL/min Water-Soluble Vitamins from Multi-Vitamin Tablet 9 Water-Soluble Vitamins 8. Thiamine. Ascorbic Acid. Unknown. Fumaric Acid. Pyridoxine. Niacinamide. Pantothenic Acid. Ascorbic Acid. Nicotinic Acid. Pyridoxamine. Niacinamide. Folic Acid. Riboflavin. Vitamin B Column: Prevail C8, µm, 0 x.mm Column: Platinum C8, µm, 0 x.mm (Part No. 0) (Part No. 9908) Mobile Phase: A: 0.M Ammonium Phosphate ph. Mobile Phase: 0.0M KHPO ph.0:acetonitrile (9:) B: Acetonitrile Gradient: Time: 0 8 Detector: UV at B%: 0 0 Detector: UV at nm Copyright 00 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 9

15 HPLC Applications F O O D & B E V E R A G E Carbohydrates Corn Syrup Solids 9 Corn Syrup Oligomers 90. Dextrose. Maltose. Maltotriose. Maltotetraose Maltopentaose. Maltohexaose. Maltoheptaose 8. Maltooctaose 9. Maltononaose 0. Maltodecaose. Maltoundecaose. Maltododecaose. Maltotridecaose 8. Dextrose. Maltose. Maltotriose. Maltotetraose. Maltopentaose. Maltohexaose. Maltoheptaose 8. Maltooctaose Min. Column: Prevail Carbohydrate ES, µm, 0 x.mm (Part No. 0) Mobile Phase: A: Acetonitrile B: Water Gradient: Time 0 %B Min. Column: Prevail Carbohydrate ES, µm, x mm Rocket (Part No. 0) Mobile Phase: A: Acetonitrile B: Water Gradient: Time: 0 %B:, 0 Flow Rate:.0mL/min. iso-erythritol. Fructose. Sorbitol. Mannitol. Glucose. Inositol. Sucrose 8. Maltitol Maltose 0. Raffinose. DP. DP. DP. DP. DP. DP8. DP9 8. DP0 9. DP 0. DP. DP. DP Column: Prevail Carbohydrate ES, µm, 0 x.mm (Part No. 0) Mobile Phase: A: Acetonitrile B: 0.0% Ammonium Hydroxide in Water Gradient: Time: %B: Mono-, Di-, Tri- Saccharides Beer Standards Unmodified Mobile Phase. Fructose. Glucose. Sucrose. Maltose. Maltotriose. Maltotetraose. Maltopentaose. Fructose. Glucose. Sucrose Column: Prevail Carbohydrate ES, µm, x mm Rocket (Part No. 0) Mobile Phase: Acetonitrile:Water (:) Flow Rate:.0mL/min Column Temp: 0 C. Fructose. Glucose. Sucrose. Maltose. Maltotriose. Maltotetraose. Maltopentaose Sugars Beer Standards Modified Mobile Phase Min Min. 9 Column: Prevail Carbohydrate ES, 0 x.mm (Part No. 0) Mobile Phase: A: Acetonitrile B: Water Gradient: Time: 0 0 %B: 0 Copyright 00 Alltech Associates, Inc. Column: Prevail Carbohydrate ES, 0 x.mm (Part No. 0) Mobile Phase: A: Acetonitrile:Acetone (:) B: Water Gradient: Time: 0 8 %B: 0 Contact your Alltech office or distributor for current or local prices.

16 F O O D & B E V E R A G E HPLC Applications Sugar Alcohols. Iso-erythritol. Xylitol. Mannitol. Maltitol 9 Microbore Analysis of Glucopyranosides. n-octyl Glucopyranoside (0ng on column). n-decyl Glucopyranoside (0ng on column). n-dodecyl Glucopyranoside (0ng on column) Min. Column: Prevail Carbohydrate ES, 0 x.mm (Part No. 0) Mobile Phase: Acetonitrile:Water (0:0) Column: Alltima C8, µm, 0 x mm (Part No. 888) Mobile Phase: Methanol:Water (90:0) Flow Rate: 0.0mL/min Imported Ale. Fructose. Glucose. Maltose. Maltotriose. Maltopentaose. Maltohexaose. Maltoheptaose 9 Wine Analysis Sipaska Rosé. Fructose. Sorbitol. Glucose. Maltose Min. Column: Prevail Carbohydrate ES, µm, 0 x.mm (Part No. 0) Mobile Phase: A: Acetonitrile B:Water Gradient: Time: 0 0 %B: 0 Wine Analysis by Ion Exclusion Column: Prevail Carbohydrate ES, µm, 0 x.mm (Part No. 0) Mobile Phase: A: Acetonitrile B: Water Gradient: Time: 0 0 %B: Beverage Additives 9. Citric Acid. Ascorbic Acid. Tartaric Acid. Saccharin. Glucose. Benzoic Acid. Malic Acid. Aspartame. Fructose. Vanillin. Acetic Acid. Glycerol 8. Lactic Acid 9. Methanol 0. Ethanol Column: IOA-000, 9mm, 00 x.8mm Column: Prevail C8, µm, 0 x.mm (Part No.88) (Part No. 9) Mobile Phase: Acetonitrile:0.0M Ammonium Acetate ph., (0:80) Mobile Phase: 0.00N Sulfuric Acid Flow Rate: 0. ml/min Flow Rate: 0.mL/min Detector: UV at 0 Column Temp: 0 C Detector: RI Copyright 00 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 9

17 HPLC Applications F O O D & B E V E R A G E Organic Acids Organic Acid Mix 98. Oxalic Acid. Tartaric Acid. Formic Acid. Malic Acid. Lactic Acid. Acetic Acid. Diglycollic Acid 8. Maleic Acid 9. Citric Acid 0. Succinic Acid. Fumaric Acid Rye Bread Extract 98. Tartaric Acid. Malic Acid. Lactic Acid. Citric Acid. Fumaric Acid Column: Prevail Organic Acid, µm, 0 x.mm (Part No. 880) Mobile Phase: 0.0M KHPO ph. Flow Rate:.mL/min Detector: UV at 0nm Column: Prevail Organic Acid, µm, 0 x.mm (Part No. 880) Mobile Phase: 0.0M KHPO ph. Detector: UV at 0nm Underivatized Fatty Acids. Lauric Acid. Myristic Acid. Palmitic Acid. Stearic Acid. Arachidic Acid. Behenic Acid 99 Organic Acid Mix. Tartaric Acid. Malic Acid. Lactic Acid. Diglycolic Acid. Maleic Acid. Succinic Acid. Fumaric Acid Min Min. 98 Column: Prevail Organic Acid, µm, 0 x.mm (Part No. 880) Mobile Phase: Acetonitrile:Methanol (:) Short-Chain Aromatic Carboxylic Acids Column: OA-000,.µm, 00 x.mm (Part No. 908) Mobile Phase: 0.0N Sulfuric Acid Column Temp: C Flow Rate: 0.8mL/min Detector: UV at 8nm Copyright 00 Alltech Associates, Inc. 0. Oxalic Acid. Citric Acid. Shikimic Acid. Fumaric Acid. Butyric Acid. Homoprotocatechuic Acid. Gallic Acid 8. Protocatechuic Acid 9. Gentisic Acid 0. p-hydroxybenzoic Acid. Benzoic Acid. Salicylic Acid Column: Platinum EPS C8, µm, 0 x.mm (Part No. 0) Mobile Phase: 0.0M KHPO ph.:methanol (9:) Flow Rate: 0.mL/min Detector: UV at 0nm Fatty Acids Using UV and ELSD in Tandem. Caproic Acid. Caprylic Acid. Capric Acid. Lauric Acid. Linolenic Acid Column: Alltima HP C8 HiLoad, µm, 0 x.mm (Part No. 88) 00. Myristic Acid. Linoleic Acid 8. Palmitic Acid 9. Oleic Acid 0. Stearic Acid Mobile Phase: A: 0.% TFA in Water B: 0.% TFA in Acetonitrile Gradient: Time: %B: Column Temp: C Contact your Alltech office or distributor for current or local prices.

18 F O O D & B E V E R A G E HPLC Applications Long Chain Fatty Acids. Capric Acid. Lauric Acid. Myristic Acid. Palmitic Acid. Stearic Acid Triglycerides Olive Oil LLO. LLP. OOL. POL. PPL. OOO. OOP 8. PPO 9. OOS Column: Prevail C8, µm, x mm Rocket (Part No. 999) Mobile Phase: Acetonitrile:Methanol (:) Flow Rate:.mL/min Column: Alltima C8, µm, 0 x.mm (Part No. 88) Mobile Phase: A: Dichloromethane B: Acetonitrile Gradient: Time: %B: Flow Rate:.mL/min Sesame Seed Oil Walnut Oil. LLL. LLO. LLP. OOL. POL. PPL. OOO 8. OOP 9. PPO 0. OOS 9. Trilaurin (LaLaLa). Trilinolein (LLL).,-Dilinoleyl--Oleyl-rac glycerol (LLO).,-Dilinoleyl--Palmitinrac-glycerol (LLP). -Palmitin--Olein-- Linolein rac-glycerol (POL).,-Dipalmytyl--Linoleinrac glycerol (PPL) 9 Column: Alltima C8, µm, 0 x.mm (Part No. 88) Mobile Phase: A:Dichloromethane B:Acetonitrile Gradient: Time: %B: Flow Rate:.mL/min Evening Primrose Oil Column: Alltima C8, µm, 0 x.mm (Part No. 88) Mobile Phase: A: Dichloromethane B: Acetonitrile Gradient: Time: %B: Flow Rate:.mL/min Additives Oxygen Heterocycles in Confectionary Products. LGG. LGL. LLL. OGL. PGL. OLL. OGO + PLL 8. OOO. DHHM Furaneol. Cyclotene. HMF. HMP. Maltol Reference: Dr. A. Lea and Dr. G. Ford, Reading Scientific Services Ltd., Reading, UK. Column: Alltima C8, µm, 0 x.mm (Part No. 880) Column: Hypercarb, µm, 00 x.mm (Part No. 980) Mobile Phase: A: Acetonitrile B: Dichloromethane Mobile Phase: A: Perchloric Acid ph.0 Gradient: Time: 0 0 B: Acetonitrile %B: 0 0 Gradient: Time: 0 0 % B: 0 Detector: UV at 80nm Copyright 00 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 99

19 HPLC Applications L I F E S C I E N C E Proteins & Peptides/Reversed Phase Protein Mix. Ribonuclease A. Cytochrome C. Lysozyme Impurity. Lysozyme. Myoglobin Impurity. Myoglobin Column: ProSphere C, 00Å, µm, 0 x.mm (Part No. 989) Mobile Phase: A: 0.% TFA B: 0.% TFA in Acetonitrile:Water (9:) Gradient: Time 0 %B 0 0 Detector: UV at 0nm Enzymes α-chymotrypsinogen. Carbonic Anhydrase Column: ProSphere C, 00Å, µm, 0 x.mm (Part No. 989) Mobile Phase: A: 0.% TFA in Water B: 0.% TFA in Acetonitrile Gradient: Time 0 %B 0 Detector: UV at 0nm Albumins. BSA. Ovalbumin 0 Protein Mix. α-lactalbumin. α-chymotrypsinogen. Carbonic Anhydrase. β-lactoglobulin 0 Column: ProSphere P-HR, µm, 0 x.mm (Part No. 08) Mobile Phase: A: 0.08% TFA in Water B: 0.0% TFA in Acetonitrile:Water (9:) Gradient: Time 0 0 %B 8 Detector: UV at 0nm Column: ProSphere P-HR, µm, 00 x.mm (Custom) Mobile Phase: A: 0.% TFA in Water B: 0.08% TFA in Acetonitrile Gradient: Time: 0 %B: 0 Detector: UV at 0nm Insulins 908. Bovine Insulin. Bovine Insulin Impurity. Porcine Insulin. Porcine Insulin Impurity Bradykinins. des-pro -bradykinin. Bradykinin Min. Column: ProSphere C8, 00Å, µm, 0 x.mm (Part No. 99) Mobile Phase: A: 0.% TFA in Water B: 0.0% TFA in Acetonitrile Gradient: Time: 0 %B: 8 8 Detector: UV at 0nm Copyright 00 Alltech Associates, Inc. Column: ProSphere C8, 00Å, µm, 0 x.mm (Part No. 99 ) Mobile Phase: A: 0.% TFA in Water B: 0.% TFA in Acetonitrile Gradient: Time: 0 %B: Detector: UV at 0nm Contact your Alltech office or distributor for current or local prices.

20 L I F E S C I E N C E HPLC Applications Bioactive Peptides 80 Peptides 90. Methionine Enkephalin. Bradykinin. Leucine Enkephalin. Physalaemin. Substance P. GLY-TYR. VAL-TYR-VAL. Methionine Enkephalin. Leucine Enkephalin. Angiotensin II Column: ProSphere C8, 00Å, µm, 0 x.mm (Part No. 99) Mobile Phase: A: 0.% TFA in Water B: 0.08% TFA in Acetonitrile:Water (9:) Gradient: Time 0 0 %B 0 0 Detector: UV at 0nm Min. Column: ProSphere C8, 00Å, µm, 0 x.mm (Part No. 99) Mobile Phase: A: 0.% TFA in Water B: 0.% TFA in Acetonitrile Gradient: Time: 0 %B: 0 0 Detector: UV at 0nm Peptide Mix. Oxytocin. Angiotensin II. Angiotensin I. Eledosin 099. Arg8-vasopressin. Bradykinin Peptide (P9) Mix. Oxytocin. Met-Enkephalin 0. Bradykinin. Luteinizing releasing hormone. Leu-enkephalin 8. Bombesin 9. Substance P Column: ProSphere C8, 00Å, µm, 0 x.mm (Part No. 08) Mobile Phase: A: 0.% TFA in Water B: 0.% TFA in Acetonitrile:Water (90:0) Gradient: Time 0 0 %B 0 0 Detector: UV at 0nm β-lactoglobulin A Tryptic Digest Column: ProSphere P-HR, µm, 0 x.mm (Part No. 08) Mobile Phase: A: 0.08% TFA in Water B: 0.0% TFA in Acetonitrile:Water (9:) Gradient: Time 0 0 %B 8 Detector: UV at 0nm Monoclonal Antibody Tryptic Digest Min. Column: ProSphere C8, 00Å, µm, 0 x.mm (Part No. 99) Column: ProSphere C8 00Å, µm, 0 x.mm (Part No. 08) Mobile Phase: A: 0.% TFA in Water, Mobile Phase: A: 0.% TFA in Water B: 0.08% TFA in Acetonitrile B: 0.% TFA in Acetonitrile Gradient: Time 0 0 Gradient: Time: 0 %B 0 %B: 0 Flow Rate: 0.mL/min Detector: UV at 0nm Detector: UV at 0nm Copyright 00 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 0

21 HPLC Applications L I F E S C I E N C E Proteins & Peptides/Reversed Phase Peptide Mix on 00Å 0 Hydrophobic Amyloid Peptides TFA Modifier at C 0. Methionine Enkephalin. Leucine Enkephalin. Angiotensin II. Neurotensin. Angiotensin I. Substance P Aβ(-8) A - 0.% TFA in Water B % TFA in 80% Acetonitrile Gradient -% B in 0min Aβ(-0) Aβ(-/) Min. Column: ProSphere C8, 00Å, µm, 0 x.mm (Part No. 99) Mobile Phase: A: 0.% TFA in Water B: 0.08% TFA in Acetonitrile Gradient: Time: 0 0 %B: 0 0 Detector: UV at 0nm Peptide Mix on 90Å 09. Methionine Enkephalin. Leucine Enkephalin. Angiotensin II. Neurotensin. Angiotensin I. Substance P Column: Flow Rate: Detector: ZORBAX 00 Extend-C8,.µm, 0 x.mm (Part No. 0-90) 0.mL/min UV at 0nm Hydrophobic Amyloid Peptides TFA Modifier at 80 C Aβ(-8) Aβ(-0) Aβ(-) Aβ(-) 0 A - 0.% TFA in Water B % TFA in 80% Acetonitrile Gradient 9-% B in 0min Column: Alltima HP C8, 90Å, µm, 0 x.mm (Part No. 89) Mobile Phase: A: 0.% TFA in Water B: 0.08% TFA in Acetonitrile Gradient: Time 0 0 %B 0 0 Detector: UV at 0nm Min. Column: ZORBAX 00 Extend-C8,.µm, 0 x.mm (Part No. 0-90) Flow Rate: 0.mL/min Detector: UV at 0nm Peptide Mix on 00Å 00. Methionine Enkephalin. Leucine Enkephalin. Angiotensin II. Neurotensin. Angiotensin I. Substance P Aβ(-8) Hydrophobic Amyloid Peptides NHOH Modifier at C Aβ(-) Aβ(-0) A - 0mM NHOH in Water B - 0mM NHOH in 80% Acetonitrile Gradient -8% B in 0min Aβ(-) 0 0 Column: ProSphere C8, 00Å, µm, 0 x.mm (Part No. 09) Mobile Phase: A: 0.% TFA in Water B: 0.08% TFA in Acetonitrile Gradient: Time 0 0 %B 0 0 Detector: UV at 0nm Copyright 00 Alltech Associates, Inc Min. Column: Flow Rate: Detector: ZORBAX 00 Extend-C8,.µm, 0 x.mm (Part No. 0-90) 0.mL/min UV at 0nm Contact your Alltech office or distributor for current or local prices.

22 L I F E S C I E N C E HPLC Applications Proteins & Peptides/Ion Exchange Protein on a Weak Anion Exchanger 08 Proteins on a Weak Cation Exchanger 0. Myoglobin. Conalbumin. Bovine Serum Albumin. β-lactoglobin A. Myoglobin. Ribonuclease A. α-chymotrypsinogen. Cytochrome C. Lysozyme Column: ProSphere P-WAX, 0µm, x.mm (Part No. P0) Mobile Phase: A: 0.0M Tris ph8 B: 0.0M Tris, M Sodium Acetate ph8 Gradient: Time 0 0 %B Flow Rate: ml/min Detector: UV at 80nm Column: ProSphere P-WCX, 0µm, x.mm (Part No. P0) Mobile Phase: A: 0.0M Potassium Phosphate ph B: 0.0M Potassium Phosphate, 0.M Sodium Chloride ph Gradient: Time 0 0 %B 0 0 Detector: UV at 80nm Proteins on a Strong Anion Exchanger. Myoglobin. Conalbumin. Bovine Serum Albumin. β-lactoglobin A 08 Proteins on a Strong Cation Exchanger 0. Myoglobin. Ribonuclease A. α-chymotrypsinogen. Cytochrome C. Lysozyme Column: ProSphere P-SAX, 0µm, x.mm (Part No. P0) Mobile Phase: A: 0.0M Tris ph8 B: 0.0M Tris,.0M Sodium Acetate ph8 Gradient: Time 0 0 %B Detector: UV at 80nm Strong Anion Exchange of Egg White 00 Column: ProSphere P-SCX, 0µm, x.mm (Part No. P0) Mobile Phase: A: 0.0M Potassium Phosphate ph B: 0.0M Potassium Phosphate, 0.M Sodium Chloride ph Gradient: Time 0 0 %B 0 0 Detector: UV at 80nm High Speed Human Hemoglobin Analysis 09 Column: ProSphere P-SAX-HR, µm, 0 x.mm (Part No. P0) Mobile Phase: A: 0.0M Tris-HCl ph 8.0 Column: ProSphere P-SCX-NP,.µm, x.mm (Part No. P0) B: 0.0M Tris-HCl,M NaCl ph 8.0 Mobile Phase: A: 0.0M NaCl, 0.0M bis-tris-hcl ph.0 Gradient: Time 0 B: 0.M NaCl, 0.0M bis-tris-hcl ph.0 %B 0 00 Gradient: Time 0 %B 0 00 Detector: UV at 80nm Flow Rate:.mL/min Column Temp: C Detector: Vis at nm Copyright 00 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 0

23 HPLC Applications L I F E S C I E N C E Proteins & Peptides/Size Exclusion Proteins (<0,000 DA) by SEC 0. Ferritin. Conalbumin. Ovalbumin. Carbonic anhydrase. Myoglobin. Cytochrome C. GLY-TYR Proteins/Hydrophobic Interaction Proteins by HIC 088. Myoglobin. Ribonuclease A. Lysozyme. α-chymotrypsin. α-chymotrypsinogen Column: ProSphere SEC, µm, 00 x.8mm (Part No. P0) Mobile Phase: 0.M Sodium Chloride, 0.0M Potassium Phosphate ph.0 Detector: UV at nm Proteins (<0,000 DA) by SEC. Thyroglobulin. IgG. Phosphorylase b. Ovalbumin. Ribonuclease A. GLY-TYR Min. Column: ProSphere P-HIC Ether, 0µm, x.mm (Part No. P0) Mobile Phase: A:.8M Ammonium Sulfate, 0.M Potassium Phosphate ph.0 B: 0.M Potassium Phosphate ph.0 Gradient: Time 0 0 %B 0 00 Detector: UV at 80nm Proteins by HIC 089. Myoglobin. Ribonuclease A. Lysozyme. α-chymotrypsin. α-chymotrypsinogen Column: ProSphere SEC 0, µm, 00 x.8mm (Part No. P0) Mobile Phase: 0.M Sodium Chloride, 0.0M Potassium Phosphate ph.0 Detector: UV at nm Purified Human Immunoglobulin M on Size Exclusion 000 Column: ProSphere P-HIC Phenyl, 0µm, x.mm (Part No. P00) Mobile Phase: A:.8M Ammonium Sulfate, 0.M Potassium Phosphate ph.0 B: 0.M Potassium Phosphate ph.0 Gradient: Time 0 0 %B 0 00 Detector: UV at 80nm Proteins by HIC 090. Myoglobin. Ribonuclease A. Lysozyme. a-chymotrypsin. a-chymotrypsinogen 0 Column: ProSphere SEC 0, µm, 00 x.8mm (Part No. P0) Mobile Phase: 0.M NaCl, 0.M Phosphate ph.0 Flow Rate: 0.8mL/min Detector: UV at 80nm Copyright 00 Alltech Associates, Inc Min. Column: ProSphere P-HIC NP-Butyl,.µm, x.mm (Part No. P0) Mobile Phase: A:.M Ammonium Sulfate, 0.M Potassium Phosphate ph.0 B: 0.M Potassium Phosphate ph.0 Gradient: Time 0 %B 0 00 Detector: UV at 80nm Contact your Alltech office or distributor for current or local prices.

24 L I F E S C I E N C E HPLC Applications Peptides/Ion Exchange Angiotensins by Cation Exchange Proteins /Affinity Affinity purification of trypsin Unretained Impurities. Purified Trypsin Column: ProSphere P-SCX-HR, µm, 0 x.mm (Part No. P0) Mobile Phase: A: 0.0M Sodium Acetate ph.0 B: M NaCl, 0.0M Sodium Acetate ph.0 Gradient: Time: 0 0 %B 0 00 Detector: UV at 80nm Amino Acids Amino Acids without Derivatization. Gly. Ser/Asn. Asp. Gln Ala/Thr. Glu. Cys/Lys 8. His 9. Pro 0. Arg. Val. Met. Tyr. Ile. Leu. Phe. Trp Column: ProSphere ABA, 0µm, x.mm (Part No. P0) Mobile Phase: A: 0.M NaCl, mm CaCl, 0.0M Tris-HCl ph 8.0 B: 0.M NaCl, mm CaCl, 0.0M Glycine-HCl ph.8 Gradient: Time %B Detector: UV at 80nm Thyroid Chemicals 9. DL-Tyrosine. -Iodo-Tyrosine 88.,- Diiodo-D-Tyrosine. DL-Thyroxine.,,-Triiodo-L-Thyronine Min. Column: Prevail C8, µm, 0 x.mm (Part No. 990) Mobile Phase: A: mm Heptafluorobutyric Acid, 0.% TFA B: Acetonitrile Gradient: Time: 0 8 %B: 0 0 Phenylalanine O Min. Column: Platinum EPS C8, µm, 0 x.mm (Part No. ) Mobile Phase: 0.0M KHPO, ph.:acetonitrile (:) Detector: UV at nm Catechols Catecholamines. VMA. -HIAA. HVA 08 NH OH 8 0 Min. Column: Chirobiotic T, 0 x.mm (Part No. 0) Column: Inertsil CN-, µm, 0 x.mm Mobile Phase: Ethanol:Water (80:0) (Part No. GL-090) Mobile Phase: 0.0M NaHPO ph.0:acetonitrile (99:) Detector: UV at 0nm Column Temp: 0 C Detector: ECD, 00 mv vs. Ag/AgCl Copyright 00 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 0

25 HPLC Applications L I F E S C I E N C E Nucleic Acids Nucleosides. Cytidine. Uridine. Xanthosine. Guanosine. Adenosine 0. Cytidine. Uridine. Xanthosine. Guanosine. Adenosine Nucleosides 8 Column: Alltima HP EPS C8, µm, 0 x.mm (Part No. 8) Mobile Phase: A: 0.0M Potassium Phosphate ph. B: Acetonitrile Gradient: Time 0 0 %B 0 Detector: UV at 0nm Column: Platinum EPS C8, µm, 0 x.mm (Part No. ) Mobile Phase: A: 0.0M KHPO ph. B: Acetonitrile Gradient: Time: 0 0 %B 0 Flow Rate: 0.mL/min Detector: UV at 0nm Nucleosides 90 Nucleic Acid Bases 9. Cytidine. Uridine. Xanthosine. Guanosine. Adenosine. Cytosine. Uracil. Guanine. Adenine. Thymine Column: Prevail C8, µm, 0 x.mm (Part No. 9908) Mobile Phase: 0.0M KHPO ph.0:acetonitrile:(9:) Flow Rate: ml/min Detector: UV at Column: Prevail C8, µm, 0 x.mm (Part No. 9908) Mobile Phase: 0.0M KHPO ph.0:acetonitrile:(98:) Detector: UV at Nucleosides by Affinity. Cytidine. Uridine. Guanosine. Adenosine Fast High Resolution Separation of DNA Fragment Digest 09. base pairs 0. base pairs. base pairs. base pairs 09. base pairs. 9 base pairs. 80 base pairs. base pairs. 89 base pairs. base pairs. 0 base pairs. 0 base pairs. base pairs. 0 base pairs 8. base pairs. 8 base pairs 9. 8 base pairs 8. 8 base pairs 0 Column: ProSphere Boronate, 0µm, x.mm (Part No. P0) Mobile Phase: 0.M Phosphate, ph 8.0 Detector: UV at 80nm Copyright 00 Alltech Associates, Inc. Column: ProSphere P-WAX-NP,.µm, x.mm (Part No. P09) Mobile Phase: A: 0.0M Tris-HCl ph 9.0 B: M NaCl, 0.0M Tris-HCl ph 9.0 Gradient: Time 0 %B 8 Flow Rate:.mL/min Detector: UV at 0nm Contact your Alltech office or distributor for current or local prices.

26 L I F E S C I E N C E HPLC Applications Total E.coli RNA by Anion Exchange 00 Miscellaneous Phospholipids.,-Phosphatidyl-ethanolamine. Phosphatidyl-DL-glycerol. Phosphatidylinositol.,-Phosphatidyl-L-serine. Phosphatidylcholine. Sphingomyelin 0 Column: ProSphere P-WAX, 0µm, x.mm () (Part No. P0) Mobile Phase: A: 0.M NaCl, 0.M Tris-HCl ph. B:.0M NaCl, 0.M Tris-HCl ph. Gradient: Time 0 90 %B 0 0 Detector: UV at 0nm Column: Prevail Silica, µm, 0 x.mm (Part No. 99) Mobile Phase: A: Isopropanol B: Hexane C: Water Gradient: Time 0 %B %C 8 8 Benzyl Acids 98 Aromatic Acids. -Amino--hydroxybenzoic Acid. -Hydroxybenzoic Acid. Benzoic Acid. -Phenylbutyric Acid. -Phenylbutyric Acid. DL--Hydroxy--methoxy-mandelic Acid. Homovanillic Acid. Vanillic Acid. Salicylic Acid. Benzoic Acid Min. Column: Prevail Organic Acid, µm, 0 x.mm (Part No. 880) Mobile Phase: 0.0M KHPO ph.:acetonitrile (0:0) Detector: UV at nm Min. Column: Alltima C8, µm, 0 x.mm (Part No. 880) Mobile Phase: 0.0M KHPO ph.:methanol (0:0) Detector: UV at nm Monoaromatics Alcohols Uracil. Glycerol. Benzamide. Methanol. Benzyl Alcohol. Ethanol. Phenol. iso-propanol. Methylbenzoate. tert-butanol. Acetophenone. Propanol. N,N-diethyl-m-toluamide. sec-butanol 8. Toluene 8. iso-butanol 9. Benzophenone 9. tert-pentanol 8 0. Biphenyl 0. Butanol. iso-pentanol. Pentanol 0 Min. Column: Platinum EPS C8, µm, x mm Rocket Column: OA-000, 9µm, 00 x.mm (Part No. 90) (Part No. 0) Mobile Phase: 0.0N Sulfuric Acid Mobile Phase: 0.0M KHPO ph :Methanol (0:0) Flow Rate: 0.8mL/min Flow Rate:.mL/min Column Temp: C Detector: UV at nm Detector: RI Copyright 00 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 0

27 HPLC Applications E N V I R O N M E N TA L Pesticides Organophosphates 0. Methyl parathion. Dichlorvos. Ethoprophos. Azinphos Organochlorines. Methoxychlor. Dieldrin. Heptachlor.,-DDT Min. Column: Alltima HP EPS C8, µm, 0 x.mm (Part No. 8) Mobile Phase: Water:Methanol (0:0) Detector: UV at nm Triazine Herbicides 8 Column: Platinum C8, µm, 0 x.mm (Part No. 0) Mobile Phase: 0.0M KHPO ph:methanol (:) Detector: UV at 0nm Herbicides 0. Simazine. Simetryn. Prometon. Atrazine. Ametryn. Prometryn. Terbutryn. Fenuron. Monuron. Diuron. Linuron Column: Platinum EPS C8,.µm, x mm Rocket (Part No. 0) Mobile Phase: 0.0M KHPO ph:acetonitrile (:) Flow Rate:.0mL/min Detector: UV at nm 0 8 Min. Column: Alltima HP CN, µm, 0 x.mm (Part No. 89) Mobile Phase: Water:Acetonitrile (0:0) Flow Rate:.0 ml/min Detector: UV at nm Herbicides & Fungicides Bipyridylium Herbicides Asulam. MCPP. Oxine-copper. Thiuram. Iprodione. Bensulide. Pencycuron. Diquat. Paraquat 08 Column: Inertsil ODS-, µm, 0 x.mm (Part No. GL-0) Mobile Phase: 0.0M KHPO ph:acetonitrile (0:0) Column Temp: 0 C Detector: UV at 0nm Copyright 00 Alltech Associates, Inc. 0 8 Min. Column: Platinum Silica 00Å µm, 0mm x.mm (Custom) Mobile Phase: 0.0M Tetramethylammonium Hydroxide and 0.M Ammonium Sulfate in Water ph.0 with M Sulfuric Acid Flow Rate: 0.mL/min Detector: UV at 0nm until minutes UV at 0nm after minutes Contact your Alltech office or distributor for current or local prices.

28 E N V I R O N M E N TA L HPLC Applications PAHs Polyaromatic Hydrocarbons Benzene. Naphthalene. Acenaphthylene. Fluorene. Acenaphthene. Phenanthrene. Anthracene 8. Fluoranthene 9. Pyrene 0. Benz[a]anthracene. Chrysene. Benzo[b]fluoranthene. Benzo[k]fluoranthene. Benzo[a]pyrene. Dibenz[a,h]anthracene. Benzo[g,h,i]perylene. Indeno[,,,c,d]pyrene Min. Column: Prevail C8, µm, 0 x.mm (Part No. 9908) Mobile Phase: A: Water B: Acetonitrile Gradient: Time: 0 %B: 00 Flow Rate:.mL/min Detector: UV at Polynuclear Aromatic Hydrocarbons 0. Naphthalene. Acenaphthylene. Acenaphthene. Fluorene. Phenanthrene. Anthracene. Fluoranthene 8. Pyrene 9. Benz[a]anthracene 0. Chrysene. Benzo[b]fluoranthene. Benzo[k]fluoranthene. Benzo[a]pyrene. Dibenz[a,h]anthracene. Benzo[g,h,i]perylene. Indeno[,,,c,d]pyrene Column: Inertsil ODS-P, µm, 0 x.mm (Part No. GL-000) Mobile Phase: A: Water:Methanol (0:0) B: Methanol Gradient: Time: 0 0 %B: Column Temp: 0 C Detector: UV at PAH (EPA 0) 0. Naphthalene. Acenaphthylene. Acenaphthene. Fluorene. Phenanthrene. Anthracene. Fluoranthene 8. Pyrene 9. Benz[a]anthracene 0. Chrysene. Benzo[b]fluoranthene. Benzo[k]fluoranthene. Benzo[a]pyrene. Dibenz[a,h]anthracene. Benzo[g,h,i]perylene. Indeno[,,,c,d]pyrene Min. Column: Hypersil Green PAH, µm, 00 x.mm (Part No. 98) Mobile Phase: A: Water:Acetonitrile (99:) B: Water:Acetonitrile(:99) Gradient: Time: 0 %B: Flow Rate:.0mL/min Detector: UV at nm Copyright 00 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 09

29 HPLC Applications E N V I R O N M E N TA L Water Pollutants Semipolar & Highly Hydrophilic Wastewater Contaminants 8 Column: Platinum EPS C8, µm, 0 x.mm (Part No. ) Mobile Phase: A: 0.0M NaHPO ph. B: Acetonitrile:Methanol:Water (0:0:0) Gradient: Time: %B: Detector: UV at 0nm. -Amino--hydroxy-,-naphthalene-disulfonic Acid. Hydroquinone. Benzylamine. '-Aminoacetophenone. Phenol. -Hydroxyphenylacetic Acid. -Naphthalene Sulfonic Acid 8.,-Dihydroxybenzoic Acid 9. -Naphthalene Sulfonic Acid Degradant 0. -Hydroxy--methoxybenzoic Acid. Phenylacetic Acid. m-cresol. -Hydroxycinnamic Acid. trans-cinnamaldehyde. trans-cinnamaldehyde Degradant. trans-cinnamaldehyde Degradant. -Naphthalenethiol Degradant 8. -Naphthalenethiol 9. -Naphthalenethiol Degradant Phthalates Phthalic Acid Dialkyl Esters 09. diethylester. di-isopropylester. di-n-butylester. di-isobutylester. dicyclohexylester. di-n-heptylester. di--ethylhexylester 8. di-n-decylester 9. Phthalic Acid di-n-dodecylester Phenols Endocrine Disruptors. Phenol. Diethylphthalate. Bisphenol-A.,-Dichlorophenol. -Octylphenol. -Nonylphenol Column: Inertsil C8-, µm, 0 x.mm (Part No. GL-0900) Mobile Phase: A: Water B: Acetonitrile Gradient: Time: 0 0 %B: 00 Column Temp: 0 C Detector: UV at Column: Inertsil CN-, µm, 0 x.mm (Part No. GL-09) Mobile Phase: 0.M KHPO ph.0:acetonitrile (:) Column Temp: 0 C Detector: UV at 80 Nitroaromatics Explosives Nitroaromatics (Normal phase) Nitroaromatic and Nitroamine Explosives. Nitrobenzene.,-Dinitrotoluene.,-Dinitrobenzene. -Nitrophenol 08. HMX. RDX. TNB. DNB. Tetryl. NB. TNT 8. -A-DNT 9.,DNT 0.,DNT. -NT. -NT -NT Min. Column: Alltima HP CN, µm, 0 x.mm (Part No. 89) Mobile Phase: Hexane:Isopropanol (8:) Detector: UV at nm Copyright 00 Alltech Associates, Inc. Column: Platinum C8, µm, 0 x.mm (Part No. 0) Mobile Phase: Water:Methanol (0:0) Flow Rate: 0.mL/min Column Temp: 0 C Detector: UV at nm Contact your Alltech office or distributor for current or local prices.

30 E N V I R O N M E N TA L / M I S C E L L A N E O U S HPLC Applications Aflatoxins Aflatoxins. Aflatoxin G. Aflatoxin G. Aflatoxin B. Aflatoxin B 0 Aflatoxins. Aflatoxin B. Aflatoxin G. Aflatoxin B. Aflatoxin G 0 Column: Inertsil ODS-V, µm, 0 x.mm (Part No. GL-080) Mobile Phase: Water:Methanol (0:0) Column Temp: 0 C Detector: UV at Paraffins Paraffin Analysis Paraffin C0-C0 09 Column: Alltima HP Silica, µm, 0 x.mm (Part No. 89) Mobile Phase: Methylene Chloride:Methanol:Water (99.:0.:0.) Flow Rate: 0.8mL/min Fire Retardants Brominated Flame Retardants 0. Hexabromo cyclododecane. Tetrabromo bisphenol A (allyl ether) Column: Alltima HP C8 HiLoad, µm, 00 x.mm (Part No. 88) Mobile Phase: Acetonitrile:Dichloromethane (0:0) Min. Column: Alltima C8, µm, 0 x.mm (Part No. 880) Mobile Phase: Water:Acetonitrile (:9) Cooking Oil Used Cooking Oil Bucky Balls Bucky Balls 0. Polymerized. C0. Polymerized. C0. Triglycerides. MW Triglycerides MW 90. Diglycerides MW 0. Monoglycerides MW Free Fatty Acids MW 00 Column: Jordi DVB, 0 Å, 00Å, 00mm x 0mm () Column: Jordi DVB, 00Å, 00 x 0mm () (Part No.s 00, 00) (Part No. 00) Mobile Phase: THF Mobile Phase: Trichlorobenzene Flow Rate:.0mL/min Flow Rate:.mL/min Column Temp: 0 C Column Temp: C Detector: UV at nm Detector: RI Copyright 00 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.

31 HPLC Applications M I S C E L L A N E O U S Polymers Polyurethane Curatives. Polytetramethylene Glycol. Ethecure 00 Polystyrene. 8.M..M. 9K. K. 98.9K. K. 0.K 8..K 9. 0K 0. 0K Column: Jordi DVB, 00Å, 00mm x 0mm () (Part No. 00) Mobile Phase: Chloroform Flow Rate:.mL/min Column Temp: 0 C Detector: RI Column: Jordi DVB, Mixed Bed Linear, 00mm x 0mm (Part No. 008) Mobile Phase: THF Flow Rate:.mL/min Column Temp: 0 C Detector: RI Polyethylene and Hydrocarbon Standards. PE-000 MW. PE- MW. C8. C. C. C. C0 8. C8 9. C 0. C. C. C0. C8. C. C. C. C0 8. C8 9. C 0. C. C. C0. C8. C. C. C. C0 8. C9 9. C8 0. C. C. C 0 Column: Jordi DVB, 00Å, 00mm x 0mm () (Part No. 00) Mobile Phase: Trichlorobenzene Flow Rate:.mL/min Column Temp: C Detector: RI Tween Dimethicone 8 Column: Alltima C8, µm, 0 x.mm (Part No. 880) Mobile Phase: Water:Methanol (0:0) Copyright 00 Alltech Associates, Inc. Column: Alltima C8, µm, 0 x.mm (Part No. 880) Mobile Phase: A: Acetonitrile B: Chloroform Gradient: Time: 0 0 %B: Contact your Alltech office or distributor for current or local prices.