Preparation of extracellular polysaccharide from fermentation liquor of marine microalgae Schizochytrium and study on the bioactivities

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1 Journal of Fuzhou University Natural Science Edition Vol 39 No 5 Oct 2011 DOI CNKI /N Schizochytrium sp TIO DP- PH Physalospora piricola Pythium aphanidermatum Botrytis cinerea Fusarium pathogens Botrytis cinerea TS209 A Preparation of extracellular polysaccharide from fermentation liquor of marine microalgae Schizochytrium and study on the bioactivities WANG Shao - yun 1 JIANG Yong 1 MENG Chun 1 OUYANG Yan - hua 1 LIN Xiang - zhi 2 1 College of Biological Science and Technology Fuzhou University Fuzhou Fujian China 2 Third Institute of Oceanography State Oceanic Administration Xiamen Fujian China Abstract The ethanol precipitation was executed to extract exopolysaccharide from the fermentation solution of marine microalgae Schizochytrium sp TIO1101 The extracted exopolysaccharide solution was then applied to 732 cationic exchange resin for further separation the elution was isolated with into three major polysaccharide components acidic polysaccharide weak alkaline polysaccharides and alkaline polysaccharides And the polysaccharides were assessed for antioxidant activity against reducing power activity DPPH radical scavenging activity and hydroxyl radical scavenging activity assays respectively The results indicated that weak alkaline polysaccharides showed significant antioxidant activity Antifungal activity experiment showed all three polysaccharide components demonstrated antifungal activity to Physalospora piricola Pythium aphanidermatum Botrytis cinerea and Fusarium pathogens to some extent particularly showing high effect on Botrytis cinerea Keywords marine microalgae exopolysaccharide antioxidant activity antifungal activity 1 2 Schizochytrium sp TIO E - mail lxz8848@ 263 net JA10012

2 Schizochytrium sp TIO DPPH WAKO Pythium aphanidermatum Physalospora piricola Botrytis cinerea Fusarium pathogens 1 2 CF15RX Hitachi Koki BSZ Thermo Forma YXQ - LS - SII ORIN ph Orion Research TCA TCA TCA 8% r min min 95% 80% 4 12 h r min min 0 2 mol L - 1 ph 7 0 Na 2 HPO mol L - 1 ph 7 0 Na 2 HPO φ1 6 cm 20 cm 0 2 mol L - 1 Na 2 HPO 4 - ph mol L - 1 Na 2 HPO 4 - ph 7 0 NaCl NaCl 0 ~ 2 0 mol L mol L - 1 ph 7 0 Na 2 HPO ml min ml / ph 6 6 1% 2 5 ml μg /ml min 10% TCA 2 5 ml 2 5 ml 1 0% FeCl ml 10 min 700 nm DPPH 10 μmol L - 1 DPP 2 ml 0 1 mmol L - 1 DPPH 2 ml 30 min 517 nm 4 DPPH % = 1 - A 1 - A 2 /A 0 100% A 0 2 ml 0 1 mmol L - 1 DPPH + 2 ml A 1 2 ml 0 1 mmol L - 1 DPPH + 2 ml A 2 2 ml + 2 ml

3 Fe % = A - A - A - A / A - A 100% A A A A A 536 nm 0 5 ml 0 75 mmol L ml 0 15 mol L - 1 ph ml 0 5 ml 0 75 mmol L - 1 FeSO ml 0 01% H 2 O min A 0 5 ml H 2 O 2 A 0 5 ml 0 75 mmol L ml 0 15 mol L - 1 ph ml 0 5 ml 0 75 mmol L - 1 FeSO ml 0 01% H 2 O min A 1 ml 0 15 mol L - 1 ph ml 1 5 ml A 1 ml 0 15 mol L - 1 ph ml A PDA 10 ml 100 mm 15 mm ~ 20 h 5 cm 0 5 cm φ6 25 mm 8 μl μg ml TCA ~ 2 0 mol L - 1 NaCl 4 1 Fig The separation result of the crude polysaccharide by 732 cationic exchange resin nm ~ 0 5 mol L - 1 NaCl 0 5 ~ 1 0 mol L - 1 NaCl 1 0 ~ 2 0 mol L - 1 NaCl 44 6% 32 3% 22 1% 0 95% 1 0 ~ 2 0 mol L - 1 NaCl

4 5 Tab The polysaccharide contents of the isolated components from 732 cationic exchange resin /% / 0 mol L - 1 NaCl ~ 0 5 mol L - 1 NaCl mol L - 1 NaCl mol L - 1 NaCl ~ 120 μg ml a a Fig 2 2 b DPPH The activity of the major components in polysaccharide c DPPH DPPH 2 b DPPH DPPH DPPH IC μg ml μg ml c DPPH IC 50 IC μg ml - 1 IC μg ml DPPH IC μg ml A 60 μg ml - 1 B

5 μg ml - 1 C 3 B A Fig 3 3 Antifungal activity of the crude polysaccharides A 60 μg ml - 1 B 120 μg ml - 1 C 4 B A Fig 4 4 Antifungal activity of the acidic polysaccharides 2 5 A 60 μg ml - 1 B 120 μg ml - 1 C A B B A Fig 5 5 Antifungal activity of the weak basic polysaccharides

6 A 60 μg ml - 1 B 120 μg ml - 1 C B A Fig 6 6 Antifungal activity of the basic polysaccharides Schizochytrium sp TIO Physalospora piricola Pythium aphanidermatum Botrytis cinerea Fusarium pathogens 1 J J Messina M J Charles L Loprinzi C L Soy for breast cancer survivors a critical review of the literaturel C / / American Institute for Cancer Research 11th Annual Research Conference on Diet Nutrition and Cancer J Alcalase J Gerhardt P M J J Wang Shao - yun Ye Xiu - yun Ng T B A chitinase with antifungal activity from the mung bean J Protein Expression and Purification Wang Shao - yun Rao Ping - fan A leguminous trypsin - chymotrypsin inhibitor Limenin with antifungal activity from Phaseolus limensis J European Food Research and Technology