Profiling intact steroid sulfates and unconjugated steroids in biological fluids by liquid chromatography-tandem mass spectrometry (LC-MS-MS)

Size: px

Start display at page:

Download "Profiling intact steroid sulfates and unconjugated steroids in biological fluids by liquid chromatography-tandem mass spectrometry (LC-MS-MS)"

Julian Taylor
6 years ago
Views:

1 S-1 SUPPORTING INFORMATION for Profiling intact steroid sulfates and unconjugated steroids in biological fluids by liquid chromatography-tandem mass spectrometry (LC-MS-MS) Christina E. Galuska, a Michaela F. Hartmann,* a Alberto Sánchez-Guijo, a Katharina Bakhaus, b Joachim Geyer, b Gerhard Schuler, c Klaus-Peter Zimmer c and Stefan A. Wudy a a Steroid Research & Mass Spectrometry Unit, Department of General Pediatrics and Neonatology, Center of Child and Adolescent Medicine, Justus-Liebig-University, Feulgenstrasse 12, Giessen, Germany. Michaela.Hartmann@uni-giessen.de b Institute of Pharmacology and Toxicology, Justus-Liebig-University, Biomedical Research Center (BFS), Schubertstrasse 81, Giessen, Germany c Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals, Justus-Liebig-University, Giessen, Frankfurter Strasse 106, Germany

2 S-2 Table S-1: Intra- and inter-assay precision of the LC-MS-MS method for the steroid sulfates in aqueous solution, cell lysate and serum. intra-assay precision inter-assay precision analyte S S 16-OH- S AS S S S S 16-OH- S AS S S in aqueous solution mean SD CV in cell lysate mean SD CV in serum mean SD CV Data are shown for the concentration level 10 ng/ml: mean in ng/ml, SD: standard deviation, CV in %, n=5

3 S-3 Table S-2a: Intra-assay precision of the LC-MS-MS method for the unconjugated steroids in aqueous solution, cell lysate and serum. 4 A T 17-OH- in aqueous solution mean SD CV in cell lysate mean SD CV in serum mean SD CV Data are shown for the concentration level 10 ng/ml: mean in ng/ml, SD: standard deviation, CV in %, n=5 Table S-2b: Inter-assay precision of the LC-MS-MS method for the unconjugated steroids in aqueous solution, cell lysate and serum. 4 A T 17-OH- in aqueous solution mean SD CV in cell lysate mean SD CV in serum mean SD CV Data are shown for the concentration level 10 ng/ml: mean in ng/ml, SD: standard deviation, CV in %, n=5

4 S-4 Table S-3: Recovery of the sample preparation procedures (lysate Chromabond C18ec extraction and serum protein precipitation/seppak C18 extraction) for the sulfated steroids lysate Chromabond C18ec extraction serum protein prec./seppak C18 extraction analyte S S 16-OH- S AS S S S S 16-OH- S AS S S exp. m mean SD CV recov mean SD CV recov exp. m mean SD CV recov exp. m: expected mean in ng/ml, mean: measured mean in ng/ml, SD: standard deviation, CV in %, recov.: recovery in %

5 S-5 Table S-4a: Recovery of the sample preparation procedures: lysate Chromabond C18ec extraction for the unconjugated steroids 4 A T 17-OH- exp. m <LoQ <LoQ <LoQ <LoQ 1.00 <LoQ <LoQ mean SD CV recov mean SD CV recov exp. m mean SD CV recov exp. m: expected mean in ng/ml, mean: measured mean in ng/ml, SD: standard deviation, CV in %, recov.: recovery in %, <LoQ: below the limit of quantification. Table S-4b: Recovery of the sample preparation procedures: serum protein precipitation/seppak C18 extraction for the unconjugated steroids 4 A T 17-OH- exp. m <LoQ <LoQ <LoQ <LoQ 1.00 <LoQ <LoQ mean SD CV recov mean SD CV recov exp. m mean SD CV recov exp. m: expected mean in ng/ml, mean: measured mean in ng/ml, SD: standard deviation, CV in %, recov.: recovery in %, <LoQ: below the limit of quantification.

6 S-6 Table S-5: Matrix effects in lysate and serum samples for the sulfated steroids matrix effect lysate matrix effect serum analyte S S 16-OH- S AS S S S S 16-OH- S AS S S mean SD CV rel. err exp. m: expected mean in ng/ml, mean: measured mean in ng/ml, SD: standard deviation, CV in %, rel. err: relative error in %.

7 S-7 Table S-6a: Matrix effects in lysate samples for the unconjugated steroids 4 A T 17-OH- mean SD CV rel. err exp. m: expected mean in ng/ml, mean: measured mean in ng/ml, SD: standard deviation, CV in %, rel. err: relative error in %. Table S-6b: Matrix effects in serum samples for the unconjugated steroids 4 A T 17-OH- mean SD CV rel. err exp. m: expected mean in ng/ml, mean: measured mean in ng/ml, SD: standard deviation, CV in %, rel. err: relative error in %.

DETERMINATION OF CANNABINOIDS, THC AND THC-COOH, IN ORAL FLUID USING AN AGILENT 6490 TRIPLE QUADRUPOLE LC/MS

FORENSICS AND TOXICOLOGY ANALYSIS DETERMINATION OF CANNABINOIDS, THC AND THC-COOH, IN ORAL FLUID USING AN AGILENT 6490 TRIPLE QUADRUPOLE LC/MS Solutions for Your Analytical Business Markets and Applications