Binders-premixture for all species of animals

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2 Binders-premixture for all species of animals Technological additives/kg: Kieselgur, diatomaceous earth, purified Bentonite Kaolinitic clays, free of asbestos Natural mixtures of steatites and chlorite (E551c) mg (1m558i) mg (E559) mg (E560) mg Dosage: For all species of animals: 0,1 0,4 % (1 4 kg per ton of feed) Turkeys and chickens for fattening: 2kg/ton of chick starter, following 1 kg/ton of broiler starter and finisher Chickens reared for laying: 1 kg/ton of chick starter during the first four weeks of life, following 1 kg/ton of feed Laying hens: 1 kg/ton of layer feed Weaning piglets, pigs for fattening, sows, boars: 2 kg/ton of complete feedingstuffs Dairy cows, cattle for fattening: g/head/day or 4 kg/ton of total dry matter Exclusively for the manufacture of feedingstuffs. May be used in organic production in accordance with Regulations (EC) 834/2007 and 889/2008. Control body: AT-BIO-301 Manufactured by: Alvetra & Werfft Animal Nutrition GmbH, A-2491 Neufeld/Leitha, Landeggerstraße 7 Approval Nr. α AT 1002 is a registered trademark of Alvetra & Werfft Animal Nutrition GmbH page 2 of 7

3 Structural elements of FIX-A-TOX Basic mechanism of action: NOT ONLY simply absorption (based on electrically charged cations/anions; electrostatic forces are rather weak; binding is reversible) Countless number of micropores of the crystals exert a capillary force like a sponge Incorporated substances enter into a chemical linkage with impurities (free radicals, hydroxy-groups, uncoordinated metals) of the crystals which now is stable and irreversible Impurities and spatial structure of the carefully chosen blend of crystals in FIX-A.TOX are designed to obtain optimum mycotoxin binding features Clinical Trials with Fix-A-Tox: The efficacy of mycotoxin binding agents can essentially only be demonstrated in animal experiments: Trial results exist from studies in pigs (and also for selected parameters in boar sperm) investigating the influence of FIX-A-TOX on reproduction and growth rates. page 3 of 7

4 The performance of Fix-A-Tox Relative amount of mycotoxins absorbed by FIX-A-TOX Mycotoxin Absorbed amount of mycotoxins, % 1. ph2 ph7 ph9 2. 6h 12h 24h 6h 12h 24h 6h 12h 24h Aflatoxin G Aflatoxin G Aflatoxin B Aflatoxin B Ochratoxin A Zearalenon DON * Sulfadimidin- Na Origin: Dipl. Ing. Margit Werther and Prof. Dr. sc. Achim Strey, Berlin, 1996 Published in Die Mühle und Mischfuttertechnik, 133. Jahrgang, Heft 48, 28. November 1996 (* at that time without DON. The DON binding rate has been investigated 1998 by the same institute) Adsorption characteristics of FIX-A-TOX with regard to vitamins and trace elements For methodological reasons, it was not possible to quantitatively investigate vitamin bonding in the above experiment. Nonetheless, over a decade of experience gathered through the use of FIX-A-TOX in large-scale animal husbandry operations with massive mycotoxins problems can be regarded as a long-term study. Since there has never been even the faintest evidence of avitaminosis, one can draw a scientifically valid conclusion that no significant nutritional-physiological binding of vitamins occurs. Due to its aluminosilicate structure, any irreversible adsorption of trace elements can be excluded. These remain freely available to animal at any time. Conclusion of Prof. Dr. sc. Achim Strey First of all, the results generally confirm the good capability of binding mycotoxines conceded to FIX-A-TOX. The strongest absorption has been observed with the Aflatoxins. Regardless the ph-values and how long they were exposed to FIX-A-TOX, the absorption of all the Aflatoxins was nearly quantitative (between 89 and 100%). All in all it can be concluded from this pilot test that FIX-A-TOX has a good to excellent potential for absorption of mycotoxines. Especially the effect on Aflatoxines is of eminent importance. page 4 of 7

5 General Information: European Legislation Commission Directive 2003/100 on undesirable substances in animal feed Undesirable Products intended for animal feed Maximum content in Substances mg/kg (ppm) relative to a feedingstuff with a moisture content of 12% Aflatoxin B1 All feed materials 0,02 Complete feedingstuffs for cattle, sheep and goats with the exception of: 0,02 - complete feedingstuffs for dairy animals 0,005 - complete feedingstuffs for calves and lambs 0,01 Complete feedingstuffs for pigs and poultry (except young animals) 0,02 Other complete feedingstuffs 0,01 Complementary feedingstuffs for cattle, sheep and goats (except com- 0,02 plementary feedingstuffs for dairy animals, calves and lambs) Complementary feedingstuffs for pigs and poultry (except young animals 0,02 Other complementary feedingstuffs 0,005 page 5 of 7

6 Commission Recommendation 2006/576/EC on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 and fumonisins in products intended for animal feeding GUIDANCE VALUES Mycotoxin Products intended for animal feed Guidance value in mg/kg (ppm) relative to a feedingstuff with a moisture content of 12% Deoxynivalenol Feed materials (*) - Cereals and cereal products (**) with the exception of maize by-products 8 - Maize by-products 12 Complementary and complete feedingstuffs with the exception of 5 - complementary and complete feedingstuffs for pigs 0,9 - complementary and complete feedingstuffs for calves (< 4 months), 2 lambs and kids Zearalenon Feed materials (*) - Cereals and cereal products (**) with the exception of maize by-products 2 - Maize by-products 3 Complementary and complete feedingstuffs - Complementary and complete feedingstuffs for piglets and gilts (young sows) 0,1 - Complementary and complete feedingstuffs for sows and fattening pigs 0,25 - Complementary and complete feedingstuffs for calves, dairy cattle, sheep 0,5 (including lamb) and goats (including kids) Ochratoxin A Feed materials (*) - Cereals and cereal products (**) 0,25 Complementary and complete feedingstuffs - Complementary and complete fedingstuffs for pigs 0,05 - Complementary and complete feedingstuffs for poultry 0,1 Fumonisin Feed materials (*) B1 + B2 - maize and maize products (***) 60 - Complementary and complete feedingstuffs for: - pigs, horses (Equidae), rabbits and pet animals 5 - fish 10 - poultry, calves (< 4 months), lambs and kids 20 - adult ruminants (> 4 months) and mink 50 (*), (**), (***).. please see the corresponding footnotes hereto of the Commission Recommendation itself page 6 of 7

7 Commission Regulation (EC) No 466/2001 setting maximum levels for certain contaminants in foodstuffs Section 2: Mycotoxins Product Maximum level (µg/kg) B1 B1 + B2 + G1 + G2 M1 2.1 AFLATOXINS Milk (raw milk, milk for the 0,05 manufacture of milk-based products and heat-treated milk as defined by Council Directive 92/46/EEC, as last amended by Council Directive 94/71/EC) Lactating animals that eat feed contaminated by Aflatoxin B1 (AFB1) excrete in milk the Aflatoxin M1 (AFM1), a metabolite of AFB1 considered a potential carcinogenic compound for humans and included in the class 2B of active carcinogenic compounds by IARC (1993). The relationship between the amount of AFB1 ingested and quantity of AFM1 in milk is quite variable. In some cases, the intake by dairy cows of AFB1 below the tolerance level led to excretion of AFM1 in milk above the EC tolerance limit. taken from: J. Dairy Sci. 86: American Dairy Science Association, 2003 page 7 of 7

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