Balancing Amino Acids An Example of a Reformulated Western Dairy Ration Brian Sloan, Ph.D.

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1 Balancing Amino Acids An Example of a Reformulated Western Dairy Ration Brian Sloan, Ph.D. To illustrate how to reduce nitrogen (N) excretion and still improve performance, a typical ration was formulated through CPM to meet the requirements of a cow producing 100 lbs. of milk 3.50% fat and 3.30% Crude Protein (CP) eating 54.1 lbs. of Dry Matter (DM) daily. Ingredient costs were California spot prices for the second week of November According to CPM the reformulated ration would Reduce urinary N excretion by more than 18% Reduce N in manure by more than 7% Increase the efficiency Feed N into milk by more than 7% Decrease CP from 17.7% to 17.1% and still Improve milk protein and fat yields by ~.15% While reducing ration cost by 16 per cow per day. The Conventional Western Dairy Ration contained typical forages, corn as the principal grain, canola and distillers as the principal protein sources with one pound of an animal protein blend to provide additional Rumen Undegradable Protein (RUP) to meet metabolizable protein (MP) requirements. Conventional Western Dairy Ration for High Producing Dairy Cow Ration Formulated to Meet Nutrient Requirements of 2 nd Lactation Cows Producing 100 lbs. with 3.50% Fat and 3.30% CP Ingredients Lbs. as Fed Ingredient Cost per Ton Alfalfa Hay Corn Silage Cane Molasses Flaked Corn Canola Meal Whole Cottonseed DDGS Animal Protein Blend Soybean Hulls Tallow Basemix Premix Total DMI = 54.1 lbs Cost per cow per day = $6.54

2 This Conventional Western Dairy Ration was slightly positive in Metabolizable Energy (ME) balance, balanced for MP, and had a reasonable Metabolizable Lysine (LYS) level of 6.48% of MP. However the LYS to Metabolizable Methionine (MET) ratio was 3.33 to 1 compared to the optimum 2.89 to 1 determined recently by Whitehouse et al. This indicates that MET levels need to be improved to exploit efficiently the LYS in the ration and all the other amino acids (AA). Conventional Dairy Ration for High Producing Dairy Cows Nutrient Balance Evaluated by CPM Version ME Balance (mcal) 3.00 MP Balance (g) 7.20 LYS (% of MP) 6.48 LYS (g) MET (% of MP) 1.95 MET (g) LYS:MET Ratio 3.33 CP % Amino Acid Balancing Leads to Improved Metabolizable Protein To fully exploit the potential of AA balancing of dairy rations, not only is it important to formulate to target levels of MET and LYS as a % of Metabolizable Protein (MP), but it is also important to take advantage of the improvement in MP utilization. Within CPM this can be done by judiciously changing the lactation protein efficiency (GO TO CONSTANTS ICON => METABOLIC EFFICIENCY => LACTATION PROTEIN). The default value within CPM is 0.65.

3 The table below shows some suggested LACTATION PROTEIN EFFICIENCY values you can use depending on the opportunities to achieve different levels of LYS and MET as a % of MP. NOTE that it is not the ratio of LYS to MET that is the primary driver to improving AA balance and, consequentially, the improvement in utilization of all the other amino acids (MP usually in excess), but the level of both MET and LYS within MP. Estimated Efficiencies of Utilization of MP at Different Levels of Amino Acid Balancing (as Calculated for CPM) LYS/MET % of MP 5.9/ / / /2.35 LYS:MET 2.89:1 2.89:1 2.89:1 2.89:1 Efficiency of Utilization of MP Amino Acid Balancing Allows Lower CP rations to be formulated In the Reformulated AA Balanced Ration, the ration was balanced to achieve a LYS to MET ratio (2.89 to 1) while still maintaining the same level of LYS as a % of MP. The lactation protein efficiency was improved by 2.5 points from 0.65 to This is a scenario where targeting higher LYS and MET as a % of MP may not be financially justified due to the minimal milk protein premium being paid. The ration was reformulated and an amino acid balanced ration was achieved at a savings of 16 cents per cow per day. The major changes were that the % of forage in the ration increased from 47.6% to 49%, whole cottonseed dropped from 3.50 lbs. to 2.5 lbs. and the inclusion of soybean hulls increased from 4 to just over 5.5 lbs. The animal protein blend dropped out of the ration in preference for protected soybean meal. Liquid MetaSmart was formulated in to supply the extra MET needed to bring the ration into balance.

4 Amino Acid Balancing Leads to Better Ration Lower CP Ration Formulated to Meet Nutrient Requirements of 2 nd Lactation Cows Producing 100 lbs. with 3.50% Fat and 3.30% CP Ingredients Conventional Amino Acid Balanced (lbs. as Fed) Alfalfa Hay Corn Silage Cane Molasses Flaked Corn Canola Meal Whole Cottonseed DDGS Animal Protein Blend / Protected SBM Soybean Hulls Tallow Basemix Premix Liquid MetaSmart 0.05 Cost per cow per day $6.54 $6.38 Not only was the amino acid balanced ration less expensive than the conventional ration, it also had a better nutrient profile and lower CP. MP balance is still close to zero when the 2.5 point improvement in MP utilization (lactation protein efficiency) is taken into consideration. This allows a savings of over 70 g in MP supply, as you need less MP to meet amino acid requirements when the MP has an improved amino acid balance. The LYS to MET ratio is improved to meet the target ratio of 2.89 / 1.0. The lysine supply was decreased slightly but this has no negative consequence as LYS was in excess in the conventional ration and could not be fully exploited because of the deficit in MET. The CP content of the amino acid balanced ration could be decreased by over half a point of protein because of the need for less MP supply. What stands out, however, is that the amino acid balanced ration will supply 7.5 g more MET. This extra MET will give enhanced performance at a lower cost.

5 Nutrient Balance Evaluated by CPM Version Conventional Amino Acid Balanced ME Balance (mcal) MP Balance (g) * LYS (% of MP) LYS (g) MET (% of MP) MET (g) LYS:MET Ratio CP % *Assumes a 2.5% point improvement in MP utilization It is important to note that when balancing rations for amino acids, we should always be striving to maximize the microbial protein contribution, first and foremost. In this example, there was actually a small numeric increase in microbial protein supply; therefore, the reduction in MP supply was all in terms of economizing on the level of supplemental RUP needed. Nutrient Balance Evaluated by CPM Version Conventional Amino Acid Balanced* Forage % Crude Protein % Microbial Protein (g) LYS (% of MP) LYS (g) MET (% of MP) MET (g) LYS:MET ratio *Assumes a 2.5% point improvement in MP utilization

6 The Reformulated AA Balanced Ration Improves Estimated Yield of Milk Components The ration was formulated for 100 lbs. of milk which would equate to the production level of the high producing group. Assuming this was a one group total mixed ration (TMR) and the herd average was 85 lbs. of milk, how will the supply of an extra 7.5 g of MET as part of an amino acid balanced ration impact milk composition? As a rule of thumb, for every one gram of supplementary MET the estimated response should be 7 g of milk protein. In this example, we could thus expect an extra 52.5 g (0.115 lbs.) of milk protein. If we assume that milk yield will not change, then an increase in milk protein % of between +0.12% to 0.15% should be observed. Increasing MET by at least 5 g also shows, on average, an increase of +0.1% points in milk fat. As we used MetaSmart as the supplementary source of MET in this example, we can also anticipate a further response in milk fat due to MetaSmart s contribution to the rumen pool of HMB, for a total increase of +0.15% in milk fat. Performance Benefits to Amino Acid Balancing The g of extra metabolizable methionine will give: 1) At least the same lbs. of milk (85lbs.) 2) An increase in milk protein (~+0.12% to 0.15%) 3) An increase in milk fat (~ +0.15%) Nitrogen Excretion is Reduced when Amino Acid Balancing In this example, by reducing the CP level in the ration, improving the efficiency of MP utilization and increasing milk protein output, there is a large impact on N economy in the animal and thus on its environment. The efficiency of Feed N into milk increases by 7% from g/d to g/d. There will be a reduction in manure N output of 7%, due uniquely to a reduction in urinary N excretion of 18%. This benefits the animal as MUN s will be lower by 1.5 to 2 points, indicating less of a N load on the animal which will favor reproduction. The reduction in N excretion can have a large positive impact on the decision making process of a producer faced with tighter controls on achieving a balance of N inputs and outputs for his operation.

7 Nitrogen Utilization Comparison of the Conventional Western Dairy Ration vs. the Reformulated AA Balanced Ration Conventional Ration AA Balanced Ration Change g/d % of intake (g/d) % of intake g/d % Change N intake % % % N for Growth 1.5.2% 1.5.2% N in Milk % % 8.5 7% N in Urine % % % N in Feces % % 1.2 N in Manure % % %

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