REBATEN. Great Taste Improvement of Stevia Sweetener DAEPYUNG

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1 REBATEN Great Taste Improvement of Stevia Sweetener DAEPYUNG

2 Product description The sweetening properties of Stevia rebaudiana have been known for centuries, especially in its native land of Paraguay. The dried leaves of Stevia rebaudiana contain about 42 per cent water-soluble constituents. The sweet compounds represent about 14 per cent constituents of dried leaves and are diterpene glycosides based on the kaurene skeleton. These are mainly comprised of stevioside; steviobioside; rebaudioside A, B, D, E; dulcoside A; and Dulcoside B. These stevia sweeteners are similar in structure in that a steviol aglycone is connected at C-4 and C-13 to mono-, di- or trisaccharides consisting of glucose and/or rhamnose residues, as shown in Figure 1. The most abundant glycoside is stevioside followed by Rebaudioside. The rest of the sweet components are present in much smaller amounts. Sweetening potencies of various diterpenes found in Stevia rebaudiana are listed in Table 1.

3 Characteristic Rebaudioside A is considered to have a taste superior to steviosides. Normally, it represents a much smaller percentage of the total weight of leaves. To alleviate this shortcoming processes aimed at producing higher yields of Rebaudioside A have been reported and new cultivars with higher concentration of Rebaudioside A have been developed using genetic engineering techniques. Rebaudioside-A, is one of the many glycosides in the stevia leaf. Rebaudioside-A has been identified as good-tasting and lacking in bitterness. DAEPYUNG has isolated the rebaudioside-a glycoside and sells it under the brand. process removes almost all of the Bitter tasting Steviosides that make up the bulk of other Stevia extracts. The result is a nearly pure Rebaudioside -A product. Typical values of other Stevia products have steviosides ranging from 50 to 80%. The Food and Drug Administration has declared a natural zero-calorie sweetener derived from th e herb stevia safe for use in foods and beverag es, clearing a path for Coca-Cola Co., PepsiCo Inc. and other companies to market it in a variety of products. The FDA's decision applies only to a highly purified form of stevia known as rebaudioside A, which have further developed it for tabletop, food and beverage use.

4 Stevioside Rebaudioside-A Molecular structure Formula C 38 H 60 O 18 C 44 H 70 O 23 Chemical Name 13-[(2-O-β-Dglucopyranosyl-β-Dglucopyranosyl)oxy] kaur- 16-en-18-oic acid, β- D-gluco pyranosyl ester 13-[(2-O-β-D- glucopyranosyl-3-o-β- D-glucopyranosylβ-Dglucopyranosyl)oxy]ka ur-6-en-8-oic acid, β- D-glucopyranosyl ester MP( ) 196~ ~244 CAS.NO Specific rotation M.W Sweetness Sweetness Quality

5 Characteristic : Use as Sweetener Benefits, Application The use of and its sweet contents (steviol glycosides) as a sweetener is simple and has various advantages over other types of sweeteners: Benefits of as sweetener 1. Sugarless and almost zero calories at normal sweetness. 2. No effect on blood sugar levels, recommended for diabetics % Natural to 250 times sweeter than sugar. 5. Physically & Chemically Stable in Food Processing 6. Non-fermentable 7. Flavor enhancer 8. No Cavities of tooth 9. Non-toxic properties in animals clinical trials, and widely used for food, medicine and cosmetics without adverse effects. 10.Safe Application for Food There are two distinct markets for high potency, low calorie sweeteners: one is specifically for the low calorie/dietetic segment, while the other is as an alternative to sugar and other nutritive sweeteners (i.e. corn syrup, fructose, glucose). Soft drinks, Cordials, Fruit juices, Ice-creams, Yoghurts, Sherbets, Cakes, Biscuits, Pastries, Pies, Jams, Sauces, Pickles, Jellies, Desserts Chewing gum, Candies, Confectioneries, Sea-foods, Vegetables, Weight-watcher diets, Diabetic diets.

6 Stevia Extract (Stevioside) Purification Rebaudioside-A Separation Purification, filtration and extraction To isolate the desired Rebaudioside-A. (meets KFDA food grade processing standards) High purity Rebaudioside-A is purified selectively from the stevia extract using high level of refining technology. Quality of rebaudioside-a depends on its purity (i.e. 73% 97% pure Rebaudioside-A) and the ratio of Rebaudioside A over Stevioside. The higher the ratio, the better the product taste. DAEPYUNG has set up its own analysis and quality control systems.

7 Analytical item Rebaudioside-A (%) min Total steviol glycoside(%)-min 97% Specification 95% 80% 80% 97% 95% 80% 60% - 95% 95% 95% Loss on drying (%) max 6% 6% 6% 6% Arsenic (%) max 1 ppm 1 ppm 1 ppm 1 ppm Lead (%) max 1 ppm 1 ppm 1 ppm 1 ppm 97% Total sweet steviol glycosides 98%, With Rebaudioside-A 97%, sweetness >300 times 95% Total sweet steviol glycosides 95%, With Rebaudioside-A 95%, sweetness >290 times 80% Total sweet steviol glycosides 95%, With Rebaudioside-A 80%, sweetness >280 times 60% Total sweet steviol glycosides 95%, With Rebaudioside-A 80%, sweetness >280 times 10 kgs /bag daepyung@daepyung.co.kr International Sales Team: Seoul office: Leaders B/D 604 #274-4 Seohyeon dong, Bundang Gu, Seongnam si, Gyeonggi Do Rep of Korea Tel:~ , Fax: ~ Factory and R&D Center: #417 Odong ri Hamchang Sangju si, Gyeongsang Bukdo Rep of Korea Tel: ~ , Fax: ~

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