Physical Properties. Silica

Transcription

1 InertSustain InertSustain InertSustain is an ultra performance sugar analysis column using a weak ion-exchanger, which can be used in a ILIC mode as well. The introduction of primary amine groups prevents anomer resolution at low temperature analysis offering easy analysis for sugar methods. In addition, the newly developed ES (Evolved Surface) silica delivers highly reproducible results with exceptional stability and durability that will maintain performance over the lifetime of the method. Physical Properties Silica : ewly Developed Silica Gel (ES Silica) Particle Size : 3 μm, 5 μm Bonded Phase : Aminopropyl Groups End-Capping : one Surface Area : 350 m /g Carbon Loading : 7.0 % Pore Size : 00 A Pore Volume : 0.85 ml/g USP Code : L8 p Range : p = ~7.5

2 Analysis of Reducing Sugars InertSustain Glucose and Maltose are known to be hard-to-elute reducing sugars. Commercially available columns often show adsorption of peaks due to the residual silanols in the packing material. Adsorption of reducing sugars will not be observed on InertSustain due to the introduction ES (Evolved Surface) silica, which has less silanols in the packing. InertSustain 4 3 Column Size: 50 mm x 4.6 mm I.D. Eluent : A)C 3 C B) A / B = 75 / 5,v / v Flow Rate :.0 ml/min Col. Temp. : 40 Detection : RI Sample :. Fructose ( mg/ml). Glucose (4 mg/ml) 3. Sucrose ( mg/ml) 4. Maltose (50 mg/ml) C Fructose C Glucose C C Sucrose C C C Maltose

3 Potassium Guaiacolsulfonates Potassium Guaiacosulfonates are used as expectorants and often contained in cold medicines Column Size : 5 μm, mm I.D. Eluent : A) C 3 B) 50 mm K P4 A / B = / 0, v / v Flow Rate :0.95 ml / min Col. Temp. :30 Detection :UV 79 nm Sample :. Guaiacol. Guaiacolsulfonate C 3 Guaiacol C 3 S K Guaiacolsulfonate InertSustain Maltooligosaccharides Column Size : 5 μm, mm I.D. Eluent : A) C 3 C B) A / B = 60 / 40, v / v Flow Rate :.0 ml / min Detection :RI Sample :. Maltose (n=0). Maltotriose (n=) 3. Maltotetraose (n=) 4. Maltopentaose (n=3) 5. Maltohexaose (n=4) 6. Maltoheptaose (n=5) C C C n

4 Ascorbic Acids omocysteine reduction method is employed in the Japanese Food Sanitation Inspection Guidline, Food Additive Test. Column Size : 5 μm, mm I.D. Eluent : A) C 3 C B) 0 mm a P 4 C) 0.03 % omocysteine D) C 3 A / B / C / D = 60 / 0 /3 / 3, v / v Flow Rate :.0 ml / min Detection :UV 70 nm Sample :. Erythorbic acid. L-Ascorbic acid InertSustain Erythorbic Acid Ascorbic Acid Glucosamine Column Size : 5 μm, mm I.D. Eluent : A) C 3 C B) A / B = 75 / 5, v / v Flow Rate :.0 ml / min Detection :RI Sample :. Xylitol. Glucosamine Glucosamine

5 InertSustain R Tocopherols (Vitamin E) 3 4 Column Size : 5 μm, mm I.D. Eluent : A) Ethyl acetate B) exane A / B = 30 / 70, v / v Flow Rate :.0 ml / min Detection :UV 90 nm R 3 R Sample :. α-tocopherol R,R,R3;C 3. β-tocopherol R,R3;C 3 R; 3. γ-tocopherol R,R3;C 3 R; 4. δ-tocopherol R3;C 3 R,R; Tetrasaccharides Acorbose, a tetrasaccharide is often used for controlling the blood sugar level for diabetes. Column Size : 5 μm, mm I.D. Eluent : A) C 3 C B) A / B = 70 / 30, v / v Flow Rate :.0 ml / min Detection :RI Sample :. Acarbose. Stachyose C C C C C C 3 C C C Stachyose Acarbose

6 Acesulfame-K Acesulfame potassium, a synthetic sweetener was analyzed under a condition based on the Japanese Food Sanitation testing method. Column Size : 5 μm, mm I.D. Eluent : A) C 3 C B) % 3 P 4 A / B = 60 / 40, v / v Flow Rate :.0 ml / min Detection :UV 30 nm Sample :. Saccharin. Acesulfame potassium InertSustain S Saccharin S K Acesulfame potassium Allantoin Allantoin is a compound often used in hand cream and eye-drop. Column Size : 5 μm, mm I.D. Eluent : A) C 3 C B) A / B = 85 / 5, v / v Flow Rate :.0 ml / min Detection :UV 0 nm Sample :. Allantoin 0 0 0