VertiSep TM PRP. HPLC Columns. VertiSep TM PRP HPLC Columns
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1 VertiSep TM PRP ph stable range 1-14 Excellent analyte recovery Very rugged, long lasting materials Great peak shape for all types on compounds Good reproducibility from column to column VertiSep TM PRP-L21 HPLC columns are 100% Polystyrene Divinylbenzene (PS-DVB) Copolymer for compatibility with equivalent high efficiency as silica-based C18 columns and ph stable range VertiSep TM PRP-L21 columns comply with USP L21 specification and ideal for reversed phase separation of biopharmaceutical and pharmaceutical applications. VertiSep TM PRP-AQ HPLC columns are Hydoxylated Polystyrene Divinylbenzene (PS-DVB-OH) Copolymer for difference selectivity by reversed phase or normal phase chromatography. VertiSep TM PRP-AQ columns can be used with 100% water with minimum shrinkage. VertiSep TM PRP-AQ columns are ideal for polar compounds. VertiSep TM PRP-C18 HPLC columns are Polyvinylaclohol-based C18 for replacement of silica-based C18 columns due to the same separation efficiency and logical elution order. VertiSep TM PRP-C18 columns are stable in ph range 2-13 solutions not possible on silica. Polyvinylalcohol offers minimum shrinkage and swelling. VertiSep TM PRP-C18 columns are ideal for small analytes, peptides and small proteins. Specifications Packing Particle Carbon Pore Surface Size Load Size Area ph (μm) (%) (Å) (m 2 /g) Range PRP-L21 5, PRP-AQ PRP-C PRP-C PRP-NH VertiSep TM PRP-C8 HPLC columns are polyvinylaclohol-based C8 for replacement of silica-based C8 columns due to the same separation efficiency and logical elution order. VertiSep TM PRP-C8 columns are stable in ph range 2-13 solutions not possible on silica. Polyvinylalcohol offers minimum shrinkage and swelling. VertiSep TM PRP-C8 columns are ideal for small analytes, peptides and small proteins. VertiSep TM PRP-NH2 HPLC columns are polyvinylalcohol-based NH2 for replacement of silica-based NH2 columns due to the same separation efficiency and logical elution order. VertiSep TM PRP-NH2 columns are stable in ph range 2-13 solutions not possible on silica. Polyvinylalcohol offers minimum shrinkage and swelling. VertiSep TM PRP-NH2 columns are ideal for analysis of saccharides in normal phase like silica-based NH2 columns but longer life-time. VertiSep TM PRP are manufactured by statistic process control of silica synthesis, bonding and column packing. The reproducible column packing method control provides exceptional efficiency, symmetry and reproducible capacity factor. 90
2 Phenoylpropanolamine Vitamin B Complex 1. Phenoylpropanolamine 2. Chlorpheniramine 3. Psudoephedrine 1. Niacinamide 10 mg 2. Pyridoxine (B6) 10 mg 3. Thiamine (B1) 10 mg 4. Riboflavin (B2) 10 mg 5. Cyanocobalamine (B12) 10 mg ACN:Water (35:65) (with 0.1% TFA) Promethazine (in Syrup) 1. Promethazine FlowH 2 O Rate: Erythromycin A: 0.1 N Perchloric Acid B: ACN Time/%B: 0/5, 10/50 2.0mL/min UV210 nm 1. EANO, erythromycin A N-oxide 2. EF, erythromycin F 3. NdMeEA, N-demethylerythromycin A 4. EC, erythromycin C 5. ED, erythromycin D 6. EE, erythromycin E 7. EA, erythromycin A 8. AEA, anhydroerythromycin A 9. PSEAEN, pseudoerythromycin A enol ether 10. EB, erythromycin B 11. EAEN, erythromycin A enol ether ACN:Water (35:65) (with 0.1% TFA) VertiSep TM PRP-L21 5μm 4.6x250mm A:ACN, B:0.2M K 2 HPO 4, ph9.0, C:H 2 O Time/%B/%C: 0/6/64, 50/6/44 50 o C UV210nm Promethazine, Codeine, Ethedrine Pyridinoline 1. Promethazine 2. Codeine 3. Ethedrine 1. Pyridinoline,3 mg/ml ACN:Water (35:65) (with 0.1% TFA) Acetonitrile:Deionized Water (1:9) with 0.1% Heptafluorobutyric Acid 2.0mL/min Ambient UV280nm 91
3 Fat-soluble Vitamins Hydrochlorothiazide 1. Vitamin K3 2. Vitamin A 3. Vitamin A acetate 4. Vitamin D2 5. Vitamin D3 6. Vitamin E acetate 7. Vitamin E 8. Vitamin K1 1. Hydrochlorothiazide VertiSep TM PRP-C18 5μm 4.6x250mm CH 3 CN:CH 3 OH (50:50) UV280nm Steroids 1. Estriol Epiestriol Estradiol 4. Esterone 5. Progesterone 25mM Sodium Phosphate buffer, ph7.0:ch3cn (60:40) Drugs 1. 5-Fluorouracil 2. Propranolol 3. Diphenhydramine 4. Hydrocortisone ACN:H 2 O (65:35) 0.7mL/min 25 o C UV215nm 25mM Sodium Phosphate buffer, ph3.0:ch 3 CN (70:30) Short Chain Fatty Acids 1. Propionic Acid 2. Valeric Acid 3. Caproic Acid 4. Enanthic Acid 5. Caprylic Acid Erythromycins 1. EANO, erythromycin A N-oxide 2. EF, erythromycin F 3. NdMeEA, N-demethylerythromycin A 4. EC, erythromycin C 5. ED, erythromycin D 6. EE, erythromycin E 7. EA, erythromycin A 8. AEA, anhydroerythromycin A 9. PSEAEN, pseudoerythromycin A enol ether 10. EB, erythromycin B 11. EAEN, erythromycin A enol ether ACN&H 2 O:H 3 PO 4 (50:50) 0.7mL/min 25 o C UV210nm VertiSep TM PRP-L21 5μm 4.6x250mm A:ACN, B:0.2M K 2 HPO 4, ph9.0, C:H 2 O Time/%B/%C: 0/6/64, 50/6/44 50 o C UV210nm 92
4 Glycerol monoesters Reverse Phase Standard Mix 1. Glyceryl Monoricinoleate 2. Glyceryl Monomyristate 3. Glyceryl Monorpalmitate 4. Glyceryl Monooleate 5. Glyceryl Monostearate 6. Glyceryl Monoricinoleate 7. Glyceryl Monoricinoleate 8. Glyceryl Monolaurate VertiSep TM PRP-AQ 5μm 4.6x250mm A: ACN, B: H2O Time/%B: 0/22, 30/0 80 o C UV200nm Slip Agents 1. Oleamide 2. Stearamide 3. Erucamide 1. Uracil 2. Methylparaben 3. Acetophenone 4. Butylparaben 5. Anisole 6. Toluene VertiSep TM PRP-AQ 5μm 4.6x250mm THF:ACN:H 2 O (30:30:40) Ambient Antiulcerative Omeprazole 1. Antiulcerative Omeprazole VertiSep TM PRP-AQ 5μm 4.6x250mm A: ACN, B: H2O Time/%B: 0/22, 30/0 80 o C ELSD VertiSep TM PRP-AQ 5μm 4.6x150mm MeoH:ACN:THF:H 2 O (10:20:20:50) 50 o C Peptides Gastrin 1. Substance P 2. ß-Endorphin 3. Insulin B chain 4. Insulin 1. ß-Endorphine 2. Insulin 3. Big gastrin VertiSep TM PRP-C8 5μm 4.6x150mm 0.5% TFA:CH 3 CN (73:27) VertiSep TM PRP-C8 5μm 4.6x150mm 0.5% TFA:CH 3 CN (72:28) 93
5 Corn Syrup Solids 1. Dextrose 2. Maltose 3. Maltotriose 4. Maltotetraose 5. Maltopentaose 6. Maltohexaose 7. Maltoheptaose 8. Maltooctaose 9. Maltononaose 10. Maltodecaose 11. Maltoundecaose 12. Maltododecaose 13. Maltotridecaose Sugar and Sugar Alcohols 1. L-Rhamnose 2. D(+)-Arabitol 3. Sorbitol 4. d-galactose 5. Sucrose 6. Lactose A: ACN, B: H 2 O Time/%B, 0/35, 15/50 Beer Standards Modified Mobile Phase 1. Fructose 2. Glucose 3. Sucrose 4. Maltose 5. Maltotriose 6. Maltotetraose 7. Maltopentaose CH 3 CN:H 2 O (75:25) 0.9mL/min Separation of Mono to Trisaccharide 1. Ribose 2. Fructose 3. Glucose 4. Sucrose 5. Maltose 6. Maltotriose VertiSep TM PRP-NH2 5μm 4.6x150mm A: Acetonitrile:Acetone (75:25), B: Water Time/%B, 0/24, 18/50 ELSD CH 3 CN:H 2 O (75:25) 0.9mL/min Sugar Alcohols OTC Antihistamine and Excipients 1. Iso-erythritol 2. Xylitol 3. Mannitol 4. Maltitol 1. Diphenhydramine 2. PEG, Gelatin 3. Sorbitan 4. Glycerol 5. Sorbitol ACN:H 2 O (70:30) ACN:0.1% Ammonium Carbonate, ph6.5 (80:20) 94
6 Ordering Information Particle I.D. Size Length Phase (μm) (mm) QTY Part No. VertiSep TM PRP L x LN-E x LN-E x LN-E x LN-E531 AQ x LO-E x LO-E521 C x LA-E x LA-E521 C x LB-E x LB-E521 NH x LF-E x LF-E521 Ordering Information Particle I.D. Size Length Phase (μm) (mm) QTY Part No. VertiSep TM PRP Guard Cartridge* PRP-L x LN-E421 PRP-AQ x LB-E421 PRP-C x LB-E521 PRP-C x LF-E421 PRP-NH x LF-E521 *Guard holder required Ordering Information Description QTY Part No. Guard Ordering Holder Information with Coupler For column I.D mm
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