Table of Contents No. Title Page The Role of Choline Esterase on Vesicle Brain Development of Chicken Embryo of Exposure by Carbofuran Insecticide

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2 Table of Contents No. Title Page 1 Cecal Goblet Cell Response on Intracellular Development of Eimeria tenella in 2 Protein Characterization of Snail Mucin (Achasin) Local Isolate as an Antibacterial Factor 3 Trypsin and Pronase Treatment on the Development of Mouse Embryos Contaminated by K99 Escherichia coli 4 The Optimation of Tyrosine Kinase Activity from Sperm Isolation of Friesian Holstein Cow 5 Insulinlike Growth Factor I (IGFI) Complex Seminal Plasma of GoatRatio Glicoprotein : Protein : Carbohydrate 6 The Role of Choline Esterase on Vesicle Brain Development of Chicken Embryo of Exposure by Carbofuran Insecticide 7 MarkerSets For Paternity And Identity Test in Pigs by MeansMicrosatellite Analysis 8 BCG Vaccination Increased ROI Secretion in Dogs Macrophage Infected with Mycobacterium tuberculosis 9 Antifertility Effect of Aqoueus Extract Betel Nut of Areca catechu: as an apoptosis agent on Testis Connective Cells of Rattus norvegicus 10 The Morphology of Mandibular and Lingual Glands of Chicken (Gallus sp) and Quail (Coturnix coturnix): with Special Refference to the Distributionand Carbohydrate Content 11 Detection Of Rabies Virus In Saliva And Brain Using Antibody From G Protein As Material Diagnostic By Indirect Double Antibody Sandwich Elisa Technique 12 Study to Rice Brand in Drying Ensilage Product from Edible Snail for Decrease Higroscopies as Feed Stuff 13 Potency Rice Straw was Deammoniated and Fermented by Celulolytic Bacteria on the Dry Matter Consumption, Body Weight Gain and Feed Conversion of Sheep 14 Cecal Goblet Cell Response on Intracellular Development of Eimeria tenella in 15 Protein Characterization of Snail Mucin (Achasin) Local Isolate as an Antibacterial Factor 16 The Role of Choline Esterase on Vesicle Brain Development of Chicken Embryo of Exposure by Carbofuran Insecticide 17 The Optimation of Tyrosine Kinase Activity from Sperm Isolation of Friesian Holstein Cow 18 Insulinlike Growth Factor I (IGFI) Complex Seminal Plasma of Goat Ratio Glicoprotein : Protein : Carbohydrate 19 MarkerSets For Paternity And Identity Test in Pigs by Means Microsatellite Analysis 20 BCG Vaccination Increased ROI Secretion in Dogs Macrophage Infected with Mycobacterium tuberculosis 21 Antifertility Effect of Aqoueus Extract Betel Nut of Areca catechu: as an apoptosis agent on Testis Connective Cells of Rattus norvegicus

3 No. Title Page 22 Trypsin and Pronase Treatment on the Development of Mouse Embryos Contaminated by K99 Escherichia coli 23 The Morphology of Mandibular and Lingual Glands of Chicken (Gallus sp) and Quail (Coturnix coturnix): with Special Refference to the Distribution and Carbohydrate Content 24 Detection Of Rabies Virus In Saliva And Brain Using Antibody From G Protein As Material Diagnostic By Indirect Double Antibody Sandwich Elisa Technique 25 Study to Rice Brand in Drying Ensilage Product from Edible Snail for Decrease Higroscopies as Feed Stuff 26 Potency Rice Straw was Deammoniated and Fermented by Celulolytic Bacteria on the Dry Matter Consumption, Body Weight Gain and Feed Conversion of Sheep 27 Cecal Goblet Cell Response on Intracellular Development of Eimeria tenella in

4 Vol. 23 No. 3 / TOC :, and page : The Role of Choline Esterase on Vesicle Brain Development of Chicken Embryo of Exposure by Carbofuran Insecticide Peranan Choline Esterase (ChE) pada Pembentukan Vesikel Otak Embrio Ayam yang Terpapar Insektisida Karbofuran Abstract 1. Epy Muhammad Luqman > Bagian Anatomi Veteriner,Fakultas Kedokteran Hewan Universitas Airlangga Jl. Muyorejo Surabaya Telp Fax / epy_fkh@unair.ac.id 2. Bambang Poernomo Soenardirahardjo > Bagian Anatomi, Fakultas Kedokteran Hewan Universitas Airlangga Jl. Muyorejo Surabaya Telp Fax Laba Mahaputra > Bagian Reproduksi Fakultas Kedokteran Hewan Universitas Airlangga Jl. Muyorejo Surabaya Telp Fax Carbofuran exposure during embryonic development inhibit ChE and lead to abnormal brain development. This research was aimed to identify the effect of carbofuran exposure to chicken embryo on brain vesicle development during embryonal stage. This research used Randomized Complete design with 3 treatments and repetition with 10 fertile eggs each. Each egg in the control group were injected 0.1 ml NaCl physiologic 0.09%. All of eggs were stored into incubator in 38oC with 60 â 80 % humidity. Observation to brain vesicle were carried out in 72 hr incubation using whole mounts technique. In 72 hr after incubation, the measurement of ChE concentration were carried out by grinding the brain vesicle and the other eggs adding 0.2 ml sterile NaCl physiologic 0.9% solution.the exposure of carbofuran in degraded dose resulted in the increase ChE which was significantly difference and no significant differences in the formation of brain vesicle in those groups. Increasing ChE concentration caused brain vesicle have responsed to carbofuran as toxic agent and subserve growth regulatory and morphogenetic functions in developing embryo. Although carbofuran can promote cholinergic activity evoke neurodevelopmental damage, but brain vesicle resistant to carbofuran as good as adult brain. Keyword : Choline, Esterase,, Chicken, Embryo,,,, Daftar Pustaka : 1. AP Alvares, (1992). Pharmacology and toxicology of carbamates. In: Clinical and Experimental Toxicology of Organophosphat and Carbamates.. Oxford : Ballantyne B. and T. C. Marrs (ed). ButterworthHeinemann Ltd 2. B Ballantyne, (1992). Overview of the biological and clinical aspects of organophosphat and carbamates. In: Clinical and experimental toxicology of organophosphat and carbamates. Oxford : Ballantyne B. and T. C. Marrs (ed). ButterworthHeinemann Ltd 3. JW Bigbee, (1999). Morphogenic role for acetylcholinesterase in axonal outgrowth during neural development. Environ : Health Perspect. 107 (S1) 4. S Brimijoin, (1999). Cholinesterases in neural development: new findings and toxicologic implications. Environ : Health Perspect. 107 (S1) 5. C Brodie, (1991). Growth factors attenuate the cholinotoxic effects of ethanol during early neuroembryogenesis in the chick embryo. : Int. J. Dev Neurosci. 9 (3) 6. California Environmental Protection Agency, (2000). Carbofuran. Public Health Goals for Chemicals in Drinking Water. California : California Environmental Protection Agency. Sacramento 7. A Dajan, (1986). Pengantar Metode Statistik. Jakarta : Jilid II. LP3S 8. MD Faiman, (1991). Covalent binding of chick embryo proteins by the alkylthiocarbamate molinate. : Toxicologist. 11(1) 9. M FaregeElawar, (1991). Development of esterase activities in the chick embryo and chicken. : Toxicologist. 11(1) 10. WHO FAO, (2000). Thiodicarb. Pesticide residues in food. Toxicology evaluations. FAO and WHO working groups. Food and Agriculture Organization and World Health Organization.. : United Nation Organization 11. KA Funk, (1994). Avian embryonic brain reaggregate culture system: Characterization for organophosphorus compound toxicity studies. : Toxicol. Appl. Pharmacol. 124 (1). 12. S Gilbert, (1988). Developmental Biology. 2nd ed.. : Sinauer Associates. Massachuset 13. O Jin, (1996). Teratogenic synergy between a thiocarbamate herbicide and an organophosphorus insecticide. : Faseb. J. 10(3) 14. KBS Kumar, (1992). Teratogenic effects of methyl parathion in developing chick embryos. : Vet and Human

5 Powered by TCPDF ( Toxicol. 34(5) 15. PG Layer, (1991). Choline esterase during development of the avian nervous system. : Cell Mol Neurobiol JM Lauder, (1999). Morphogenetic roles of acetylcholine. : Environ. Health Perspect. 107 (S1) 17. TL Lassiter, (1999). Is the fetal brain protected from gestational exposure to chlorpyrifos?. : Neurotoxicology. 20(1) 18. M Lotti, (1992). Central neurotoxicity and behavioural effects of anticholinesterases. In: Clinical and Experimental Toxicology of Organophosphat and Carbamates. Ballantyne B. and T. C. Marrs (ed). Oxford : ButterworthHeinemann Ltd 19. FC Lu, (1995). Toksikologi Dasar. Jakarta : Universitas Indonesia 20. AH Martin, (1993). Magnetic field effects on 5'nucleotidase: acetylcholinesterase and alkaline phosphatase in developing chick embryos. : Teratology 48(2) 21. A Meyer, (2003). Developmental neurotoxicity elicited by gestational exposure to chlorpyrifos: When is aenylyl cyclase a target?. : Environ Health Perspect M Misawa, (1981). Teratogenic effects of cholinergic insecticides in chick embryos.. Diazinon treatment on acetylcholinesterase and choline acetyltransferase activities. : Toxicol. Appl. Pharmacol. 57(1) 23. S Siegel, (1988). Nonparametric Statistics for The Behavioral Science. : McDrwaaHill Book Company 24. TA Slotkin, (1999). Developmental cholinotoxicants: nicotine and chlorpyrifos. : Environ. Health Perspect. 107 (S1): 25. RGD Steel, (1993). Prinsip dan Prosedur Statistika. : PT Gramedia. Pustaka Utama 26. RW Tyl, (1992). Development and reproductive toxicity of anticholinesterases. In: Clinical and Experimental Toxicology of Organophosphat and Carbamates. Ballantyne B. and T. C. Marrs (ed). Oxford : ButterworthHeinemann Ltd 27. ER Weiss, (1998). Why do neurotransmitters act like growth factors?. : Perspect. Dev. Neurobiol. 5(4)

Table of Contents No. Title Page Trypsin and Pronase Treatment on the Development of Mouse Embryos Contaminated by K99 Escherichia coli

Table of Contents No. Title Page 1 Cecal Goblet Cell Response on Intracellular Development of Eimeria tenella in 2 Protein Characterization of Snail Mucin (Achasin) Local Isolate as an Antibacterial Factor