DOGRISK. Anna Hielm-Björkman, DVM, PhD, PI, Docent
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1 DOGRISK Anna Hielm-Björkman, DVM, PhD, PI, Docent
2 DOGRISK Post docs of the group: Liisa Uusitalo, PhD (human nutrition), Sara Zaldiv DVM, Phd (genetics), Ingrid Hang (DVM; GI-diseases) Ranno Viitmaa (DVM, Neurology) PhD students of the group: Johanna Roine, MSc(animal breeding and nutrition), Riitta Seppänen (DVM, dermatology), Maritsa Palmunen (DVM ; epidemiology), Liisa Korkalo (Human nutrition, epidemiology). Leader: Anna Hielm-Björkman, PhD
3 DOGRISK: Doing clinical studies, diet intervention studies, epidemiology, FFQ, validating outcome measures, and analyzing biological samples of phenotyped dogs Dept. of Equine and Small Animal Medicine - Fac. Vet Med anna.hielm-bjorkman@helsinki.fi
4 On the edge clinical studies with or without, human relevance Artificial joint implants Feacal transplantation Epileptic, Neuropathic pain, Osteoarthritis medication/treatments New animal sedative pharmacology Horse, cow, dog, cat, rabbit, elephant to humans!
5 Food frequency questionnaire Principle: List of foods provided, frequencies asked Web-based, 47 foods, specifications in drop boxes 5 frequency options (never---daily or almost daily) Is validated against an other method Use in: Case control setup, logistic regression Pros Long-term intake Large study populations Diet-disease associations Cons Memory-dependent Less detailed Amounts crude or unknown
6 Pet dogs as an animal model for studying dietenvironment-disease associations Nutritional epidemiology in canine population combines some of the advantages of two research traditions: Animal experiments Human epidemiology Short follow-up times Ethical considerations More monotonous diets Shared environment Feeding in human control Similar health problems
7 Phenotyping with numbers Diagnostic tools: Clinical examination, lameness exam, force plate, carpet, scales, x-ray, MRI, CT, thermography Validated indexes: HCPI, F-CSI, neuro (FinFun), CADESI, CIBDAI, VAS
8 Taking and using biological samples Blood, urine, feces, biopsies, (saliva), hair Creating reference values Creating a sample bank, - mostly for own use Phenotyped samples Time for anamnesis
9 Metabolomics Analyses were done in FIMM Metabolomics Unit Serum samples from 21 dogs and urine samples from 8 dogs (unpublished)
10
11 Fe Trace elements, vitamins and fatty acids Analyses were done in Mineraalilaboratorio MILA Oy Trace elements from whole blood (Li-He) Vitamins A, E, D from serum samples Fatty acids from EDTA plasma Would need: lab for Vit B12, K2, different A,D and E vitamins/provitamins Mg K R K R
12 16 RNA samples from skin biopsies have been sent to FIMM Sequencing Unit RNA sequencing qpcr in YES RNA from skin biopsies To validate the results from RNA sequencing RNA from blood samples Evaluation of immune response using a candidate gene approach
13 Diet intervention studies in dogs Gut microbiota: BEFORE diet intervention (DI): Firmic: p=0.070 Fusobact:p=0.086 Bacterioidetes: p=0.520 (n=26) (n=16) AFTER DI: Firmic: p<0.001** Fusobact:p<0.001** Bacterioidetes: p=0.012* Dogs given an only 100% raw or dry food diet for 4-5 months, before and after DI (unpublished)
14 Diseases we work with now Osteoarthritis Atopy / allergy/ (food sensitivity) IBD (~Chron s disease, Ulcerative colitis) Epilepsy Hypothyroidism (in dogs) Hyperthyroidism (in cats) Cancer..(diabetes, heart diseases, lever and kidney diseases, eye diseases.48)
15 DOGRISK goes cancer Sniffing dogs sniffing cancer from urine samples Together with Wise nose sniffer dog association, East Finland University, HUS Dept of Oncology and Cancer clinic, Cancer society of Finland, Small innovation firms and start-ups. Validating the dogs Training for different diseases
16 Ideas towards innovations New pharmacological or nutraceutical products New registered validated outcome measures New quick-tests in analyzing New apps for canine and human health Diagnosing via currents of the body Treating via light or a sound Mobilizing the inner healing in the body anna.hielm-bjorkman@helsinki.fi Tel: +358-(0)
17 Thank you!
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