Control Arm A Arm B. either: - Placebo surgery (chemical restraint only) - Non-survival surgery, or - Survival surgery with post op analgesia
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- Sharleen Stafford
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1 How to apply the IAU s lassification of Animal Use The example study** involves the following: Animals will be bred for a specific trait, only females will be used in the research Females will be randomized to 3 groups ontrol group, Arm A and Arm ontrol Arm A Arm IM injections, venipuncture, will be before tissue is collected either: - Placebo surgery (chemical restraint only) - Non-survival surgery, or - Survival surgery with post op analgesia either: - Non-survival surgery, or - Survival surgery without post op analgesia ** Note: this is a fictitious study intended only as an example of how to apply the classification of animal use.
2 Accounting for the breeding pairs used to produce offspring. Answer: reeding pairs will not be used for research. N/A Pain or istress ategory reeding to obtain females xplanation: reeding pairs will not used for research, nor will any procedures be performed on them (e.g., genotyping, venipuncture, etc.). ount the breeding pairs in ategory. [If procedures will be performed on the breeding pairs - they are not counted under ategory ]
3 Accounting for the male offspring that will be after birth. Answer: Males will not be used for research. N/A Pain or istress ategory Males are Note: females highest pain category determined by the intended use xplanation: Males will be bred and not used for research, count the males in ategory. [Females will be bred and used in research they are not counted under ategory ]
4 ontrol group: Animals receive IM injections, blood from venipuncture and will be before tissue is collected. Answer: All procedures only involve brief pain or distress. N/A Pain or istress ategory IM Injections venipuncture before tissue collection xplanation: Since all of the procedures only involve brief pain or distress with no need for use of pain relieving drugs, count these animals in ategory
5 Arm A Placebo Surgery group: Animals will be chemically restrained and sedatives will be administered. Answer: hemical restraint. N/A Pain or istress ategory hemical Restraint IM injection of sedatives xplanation: Since the procedure involves potential for more than brief distress and there are provisions to relieve distress, count these animals in ategory 0
6 Arm A non-survival surgery group: Animals receive IM injections, venipuncture, surgery with, and will be before recovery from. Answer: surgical procedure with. No Pain or istress ategory IM Injections venipuncture Surgical procedure with prior to recovery from xplanation: Since the procedure involves potential for more than brief pain and there are provisions to relieve pain AN none of the procedures involve survival surgery, count these animals in ategory 1
7 Arm A survival surgery group: Animals receive IM injections, venipuncture, surgery with, post-op analgesia and will be before tissues are collected. Answer: surgical procedure with. YS Pain or istress ategory IM Injections venipuncture Surgical procedure with IM injection of post op analgesia before tissue collection xplanation: Since the procedure involves potential for more than brief pain and there are provisions to relieve pain AN one of the procedures involves survival surgery, count these animals in ategory 2
8 Arm non-survival surgery group: Animals receive IM injections, venipuncture, prolonged restraint without sedation, surgery with, and will be before recovery from. Answer: prolonged restraint without sedation. No Pain or istress ategory IM Injections venipuncture prolonged restraint without sedation or training Surgical procedure with prior to recovery from xplanation: Since the procedure involves unrelieved distress AN none of the procedures involve survival surgery, count these animals in ategory 0
9 Arm survival surgery group: Animals receive IM injections, venipuncture, prolonged restraint without sedation, surgery with, and will be before recovery from. Answer: prolonged restraint without sedation. Yes Pain or istress ategory IM Injections venipuncture prolonged restraint without sedation or training Surgical procedure with IM injection of post op analgesia before tissue collection xplanation: Since the procedure involves unrelieved distress AN one of the procedures involves survival surgery, count these animals in ategory 2
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