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1 Name :Eman Mohammad Khalaf, Ph.D. Current job : Post-doctoral visiting scholar in Raizada Lab, department of plant agriculture, Crop science, 50 Stone Road, Guelph, Ontario, Canada N1G 2W1. Cell phone : (Canada) : (Egypt) E. mail : ekhalaf@uoguelph.ca : eimanpharmacist@gmail.com Date of birth : 1 st of February 1977 Citizenship : Egyptian Education & Certificates Degree of doctor of philosophy in Microbiology, September, 2011, Faculty of Pharmacy, Mansoura University, Egypt. - PhD Dissertation title: Microbiological and molecular studies on clinical isolates of Pseudomonas aeruginosa resistant to carbapenems Master of Science in Microbiology, May 2007, Faculty of Pharmacy, Tanta University, Egypt. - Master Dissertation title: Microbiological and Molecular Studies on Bacterial Septicemia among Children in Delta Area. Bachelor of pharmaceutical sciences, May 1999, Grade: Very good, Faculty of Pharmacy, Mansoura University. Lab experience and research activities: Techniques used in research: - Isolation of endophytes from plant tissue. Identification of isolates using both 16S rdna and 18S rdna through matching sequences against bacterial 16S and fungal 18S Genbank using BLAST search
2 - Isolation of DNA from plant tissue. - Antimicrobial assay of isolated endophytes. - In-vitro Endophytes phenotyping. - BACTEC brand fluorescent series instrument: Blood culture system. - Microscan WalkAway instrumentation, (DADE BEHRING, USA): System used for identification and susceptibility testing of bacteria. - Vertical polyacrylamide gel electrophoresis apparatus: for analysis of outer membrane protein. - Isoelectrofocusing apparatus: for determination of pi of β-lactamases. - Schimadzu spectrofluorophotometer: for studying hydrophobic antibiotic uptake, self-promoted uptake and efflux mechanism in Gramnegative bacilli. - HALO DB-20 UV/Visible spectrophotometer: to perform kinetic studies of β-lactamases. - PCR (Polymerase Chain Reaction) using both PCR machine FPROGO2D and Rotor gene Q thermocycler: For detection of genes responsible for antibiotic resistance. M. Sc. (Master Degree): Practical work aimed to study Gram-negative sepsis in children in Delta area through cases admitted to Mansoura University Children Hospital (MUCH). The study correlates three aspects. First aspect was clinical analysis of cases including departments, age, gender, body temperature and underlying conditions. Second aspect was microbiological and molecular studies on the isolated organisms including manual and automatic identification, susceptibility testing and different mechanisms of resistance including β-lactamases production, total protein profile and efflux mechanism in Gram-negative bacilli. Finally, the third aspect was detection of endotoxin in patients serums using gel clot LAL assay and its relation to the previous two aspects. Ph. D. degree: Practical work aimed to determine mechanisms of carbapenems resistance in Ps. aeruginosa isolated from clinical sources through: microbiological detection of serine carbapenemases, metallo-βlactamases and AmpC β-lactamases producing isolates, kinetic studies on β-lactamases. Determination of the role of outer membrane proteins (OprD and OprM) in carbapenems resistance. Detection and amplification of the genes; bla VIM, bla IMP and inti-1 on plasmid DNA which encode VIM, IMP β-lactamases and class-1-integron, respectively and oprd, mexb and ampc on chromosomal DNA which encode outer membrane protein OprD, efflux pump protein MexB and AmpC β-lactamases, respectively. Determination of nucleotide sequences of bla VIM gene to show diversity of VIM enzymes, oprd gene to show inactivation mutation by comparing sequences with that of PAO1 as reference strain and ampc - 2 -
3 gene to show diversity of AmpC enzymes and carbapenemase activity of some of them. Employment 03/ present Visiting scholar in Raizada Lab, dept. of Plant agriculture, University of Guelph. 10/ /2014 Lecturer of Microbiology in Faculty of Pharmacy, Damanhour University : Lecturer of Microbiology and deputy director of quality assurance unit in Faculty of Pharmacy, Delta University. Pharmacist in Mansoura University Children Hospital, : Pharmacist in medicaments paid by government program pharmacy : Emergency pharmacist : Pharmacist in out clinics pharmacy : Pharmacist in El-Tarshoby pharmacies - Member of technical committee for purchasing medications and medical supplies in Mansoura University Children Hospital. Courses taught Introduction to Environmental health programs and basic principles of epidemiology course for undergraduate students of Faculty of Pharmacy, Damanhour University Chemotherapeutic antimicrobial agents course for undergraduate students of Faculty of Pharmacy, Damanhour University Neoplasia course for undergraduate students of Faculty of Pharmacy, Damanhour University Microbial contamination of pharmaceutical products course for undergraduate students of Faculty of Pharmacy, Damanhour University - 3 -
4 Introduction to Pharmaceutical Biotechnology course for undergraduate students of Faculty of Pharmacy, Damanhour University Parasitology course for undergraduate students of Faculty of Pharmacy, Damanhour University Technical report writing course for undergraduate students of Faculty of Pharmacy, Delta University. Publications - Accepted abstract titled" Gram Negative Sepsis in Children in Delta Area, Egypt" in Neonatal Infections-Related Mortality & Morbidity in Egypt (NIRMM) Conference held on April 15-18, The George Washington University and the National Research Centre - Wael El-Naggar, Ahmed Abdelaziz, Maysaa E. Zaki, Khaled H. Abd El Galil, Shady Shokralla, Rasha Barwa and Eman Khalaf (2011): Biochemical characterization of carbapenemases extracted from Pseudomonas aeruginosa resistant to carbapenems. N. Egypt. J. Microbiol. (29): Presentations : Postgraduate seminar entitled Pyrogen test, department of microbiology, Faculty of Pharmacy, Tanta University : Postgraduate seminar entitled Production of streptokinase, department of microbiology, Faculty of Pharmacy, Tanta University : Postgraduate seminar entitled Proteins, department of biochemistry, Faculty of Pharmacy, Tanta University : Postgraduate seminar entitled Atomic absorption, department of analytical chemistry, Faculty of Pharmacy, Tanta University : Undergraduate seminar entitled Common cold, department of pharmaceutics, Faculty of Pharmacy, Mansoura University
5 Training courses & Workshops Dec.,2013 : Participating in the module entitled Interactive Lectures in DAAD Kairo Akademie Nov.,2013 : Participating in the module entitled Proposal writing Post Doc in DAAD Kairo Akademie. November, 2013 : workshop for training on SPSS software, Damanhour University November, 2013 : workshop for training on Frontpage, Damanhour University Apr.,2013 : Participating in the module entitled How to present scientific data in DAAD Kairo Akademie / 03 / 2013 : Competing for research funds, Damanhour University / 01 / 2012 : E-learning, Mansoura University / 01 / 2012 : The university lecturer preparation course, Mansoura University, including: 1- Rights and responsibilities of faculty members. 2- Intellectual property rights. 3- Use of databases. 4- Credit hours. 5- Writing for grants. 6- Profession ethics. 7- Quality assurance and performance assessment. 8- E-learning : Attending First Berlin Science Slam in Cairo in DAAD Kairo Akademie : Participating in the module entitled Managing Conflicts using Constructive Negotiation Techniques in DAAD Kairo Akademie / 12 / 2011 : Ethics of scientific research, Mansoura University / 12 / 2011 : Professional ethics, Mansoura University / 12 / 2011 : Effective presentation skills, Mansoura University / 11 / 2011 : Quality standards in teaching, Mansoura University : Participate in training program Purchasing and stores 2000 : Participate in work shop of birth control services. Mansoura, Egypt. September, 1999 : Franco Pharma company as medical representative : In Al-Helal El-Tarshoby pharmacy : In Al-Helal El-Tarshoby pharmacy
6 Conferences Apr.,2013 : Participating in Neonatal Infections-Related Mortality & Morbidity in Egypt (NIRMM) Conference. The George Washington University and the National Research Centre Dec.,2012 : Attending First Winter School on Industrial Biotechnology in National Research Centre, Cairo, Egypt : Attending Fourth annual conference of the EgSPEN Nutrition for Better Life". Cairo Semiramis Hotel, Cairo, Egypt : Attending conference of hematology, bone marrow transplant & workshop in clinical pharmacy in Naser institute. Cairo, Egypt. Professional membership - Pharmacy Syndicate, Cairo, Egypt. - Member of Egyptian Association of Hospital Pharmacy. Computer skills UNESCO certified International Computer Driving Licence certificate (ICDL) which involve the following: 1- Basic Concepts of Information Technology 2- Using the Computer and Managing Files 3- Word Processing 4- Spread Sheets (Excel) 5- Database (Access) 6- Presentation (PowerPoint) 7- Information and Communication (Internet). Computer manipulation and statistical analysis techniques (Minitab). November April 2002: Oracle developer 6 track including: - Introduction to information system. - Introduction to programming concepts. - Introduction to Database. - Introduction to network. - Introduction to Oracle SQL& PL/SQL. - Developer PL/SQL program unit. - Developer/2000 build form I. - Developer/2000 build form II. - Developer/2000 build reports. - Graduation project. 1999: Internet. 1998: Operating system (DOS). 1989: Basic (beginner)
7 Languages Arabic: Native. English: Excellent spoken and written English. French: Fair. Honors and awards - General syndicate of pharmacists (31 st pharmacist day, 2012) honor for awarded PhD degree - Syndicate of pharmacists in Dakahleya (pharmacist day, 2012) honor for awarded PhD degree - Faculty of Pharmacy, Tanta University honor (2008) on the occasion of Activities getting master degree - Practicing sports and travelling. - ( ): Participant in Royal Gate company for computer software & hardware maintenance. - Bachelor of Quran Kareem sciences from Quran Kareem institute in Mansoura, Egypt. Original certificates and recommendation letters will be submitted upon request References - Associate Prof. Dr: Manish N. Raizada Department of Plant Agriculture, University of Guelph, 50 Stone Road, Guelph, Ontario, Canada N1G 2W1 Raizada@uoguelph.ca x53396 Website: Prof. Dr: Ahmad A. Abdelaziz (Professor of Microbiology, Faculty of Pharmacy, Tanta University. Tel: E. mail: dr_ahmed_abdelaziz@yahoo.com - Prof. Dr. Wael A. El-Naggar (Professor of Microbiology, Faculty of Pharmacy, Mansoura University). E. mail: elnaggar.wael@gmail.com - 7 -
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